FastQCFastQC Report
Fri 27 May 2016
SRR940107_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940107_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2366233
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50250.060.582781
TCAACGC62650.047.5298354
ATCAACG63200.047.11823
CAACGCA66500.044.9192125
AACGCAG67400.044.3175286
TATCAAC69650.042.8420942
GGTATCA45400.037.79211
ACGCAGA82600.036.0128447
CGCAGAG86450.034.133768
GTACATG86500.033.678841
TACATGG91450.031.5221442
ACATGGG92550.030.1165453
GCAGAGT104400.028.094029
AGAGTAC87350.025.20033310-11
GAGTACA71300.024.9830841
CATGGGG61400.024.7337064
TAAGGTG10800.024.2425885
AGTACAT69400.024.201582
GAGTACT50000.024.0352612-13
CAGAGTA92600.023.7073410-11
Produced by FastQC (version 0.11.5)