FastQCFastQC Report
Fri 27 May 2016
SRR940106_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940106_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1750679
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG19440.11104262974537307No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG19070.10892916405577492No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16000.064.359651
GTATCAA32450.061.44941
GTCGACG505.323052E-447.6187021
TCAACGC42000.046.20284
ATCAACG42550.045.6055833
CAACGCA43950.044.1515855
AACGCAG44150.043.936516
TATCAAC46600.042.151752
GTACATG103600.039.7014051
TACATGG108350.037.411562
ACATGGG108900.035.7478453
CATGGGG53700.034.9169464
ACGCAGA56800.033.830317
CGCAGAG59600.032.147588
ATGGGGA28500.028.8218445
CATGGGA60700.027.6541544
GTATAGA4100.027.5840021
ATGGGAG28500.026.7333055
GCAGAGT70000.026.5213249
GAGTACA78450.023.9762741
Produced by FastQC (version 0.11.5)