FastQCFastQC Report
Fri 27 May 2016
SRR940106_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940106_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1750679
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37870.21631606936508638No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28800.16450759962277495No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG24360.13914601134759713No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG19890.11361306098947893No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28950.079.221251
GTATCAA59150.062.944071
TCAACGC78950.046.995224
ATCAACG79400.046.8038833
TATCAAC80000.046.603072
CAACGCA82050.045.3285335
AACGCAG83350.044.547616
ACGCAGA97300.038.030867
CGCAGAG98750.037.472438
GTACATG94450.035.445731
TACATGG97500.033.657142
GCAGAGT109400.033.443869
ACATGGG97350.032.8515663
CATGGGG49900.031.9854164
TGGTATC12600.031.1963372
GAGTACT59100.028.03472512-13
AGAGTAC103100.026.88969210-11
CAGAGTA106650.026.74769410-11
GTACTTT61900.026.67048614-15
GTGGTAT16350.026.2327771
Produced by FastQC (version 0.11.5)