FastQCFastQC Report
Fri 27 May 2016
SRR940105_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940105_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2589427
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34750.047.4464451
GGTATCA25950.043.122031
ATCAACG39850.040.6274223
TCAACGC40750.039.730134
CAACGCA44600.036.566725
AACGCAG45250.036.161816
GTACATG92600.034.581991
TATCAAC47550.034.1736072
TACATGG95850.033.0989152
ACATGGG97950.031.2347013
ACGCAGA54100.029.8022737
CATGGGG69850.029.3137044
CGCAGAG57450.027.859498
GAGTACA75000.024.2056771
ATGGGGA41000.023.8084035
TCCGTTG4100.023.2191898
AGTACAT73700.022.8558622
GTAAGGT13100.022.2640134
TATTCCG4750.021.3022585
GTGTAGC16800.021.2579151
Produced by FastQC (version 0.11.5)