FastQCFastQC Report
Fri 27 May 2016
SRR940105_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940105_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2589427
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30570.11805700643424202No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33850.062.2901951
GTATCAA61850.058.8405341
TCAACGC77350.046.036094
ATCAACG77650.045.9358063
TATCAAC82550.043.7149432
CAACGCA81450.043.6925665
AACGCAG83400.042.669336
ACGCAGA95700.037.0507977
CGCAGAG99100.035.7796338
GTACATG92500.031.4877951
TACATGG95700.029.9300542
ACATGGG95800.029.338813
GCAGAGT119500.029.2733679
GTGGTAT17950.029.2007051
CATGGGG66050.026.0544914
AGAGTAC104400.025.6433810-11
GAGTACT64750.025.31308612-13
CAGAGTA110450.024.61578810-11
AGTACTT71000.023.62947312-13
TGGTATC20400.023.0606372
Produced by FastQC (version 0.11.5)