FastQCFastQC Report
Fri 27 May 2016
SRR940104_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940104_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998912
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22800.22824833418759613No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34050.059.7934881
GGTATCA23700.054.50751
TCAACGC44100.045.0871434
ATCAACG44100.044.9521523
CAACGCA45750.043.5889935
AACGCAG47200.041.978766
TATCAAC49350.041.0144232
GTACATG92450.040.052381
AGGTAAG2600.038.9242672
TACATGG99900.036.7079472
ACATGGG99200.034.8065643
ACGCAGA58050.034.024997
CGCAGAG58350.033.7514728
CTCCCGC2153.6379788E-1233.2051127
CATGGGA71750.033.1881454
ATGGGAG28150.031.7202015
GTAAGGT4150.030.1242394
CATGGGG25500.030.1157994
TAAGGTG4550.030.0912045
GGAACGA1403.0944993E-629.7492268
Produced by FastQC (version 0.11.5)