##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940104_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 998912 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.449651220527933 34.0 31.0 34.0 28.0 34.0 2 31.489751850012812 34.0 31.0 34.0 28.0 34.0 3 31.5848272920938 34.0 31.0 34.0 28.0 34.0 4 34.66070284469503 37.0 35.0 37.0 32.0 37.0 5 34.85793743593029 37.0 35.0 37.0 32.0 37.0 6 34.82467524666838 37.0 36.0 37.0 32.0 37.0 7 34.8089951867632 37.0 36.0 37.0 32.0 37.0 8 34.73104337519221 37.0 36.0 37.0 32.0 37.0 9 36.23625804875705 39.0 38.0 39.0 32.0 39.0 10-11 36.28253690014736 39.0 38.0 39.0 32.0 39.0 12-13 36.208539390857254 39.0 38.0 39.0 31.5 39.0 14-15 37.65404810433752 41.0 39.0 41.0 31.0 41.0 16-17 37.74819453565479 41.0 39.0 41.0 32.0 41.0 18-19 37.842079682694774 41.0 39.0 41.0 32.0 41.0 20-21 37.93795149122245 41.0 39.0 41.0 32.0 41.0 22-23 37.9997927745387 41.0 39.0 41.0 32.0 41.0 24-25 38.090952456272426 41.0 39.0 41.0 32.0 41.0 26-27 38.11194079158124 41.0 39.0 41.0 32.0 41.0 28-29 38.06105743048437 41.0 38.5 41.0 32.0 41.0 30-31 37.988135090978986 41.0 38.0 41.0 32.0 41.0 32-33 37.90621846569067 41.0 38.0 41.0 32.0 41.0 34-35 37.81457876169272 40.5 38.0 41.0 31.0 41.0 36-37 37.6760950914595 40.0 38.0 41.0 31.0 41.0 38-39 37.53064584267683 40.0 38.0 41.0 30.0 41.0 40-41 37.42126083178498 40.0 38.0 41.0 30.0 41.0 42-43 37.37852984046643 40.0 38.0 41.0 30.0 41.0 44-45 37.448329282259095 40.0 38.0 41.0 30.0 41.0 46-47 37.37033192113019 40.0 38.0 41.0 30.0 41.0 48-49 37.25794164050487 40.0 37.5 41.0 30.0 41.0 50-51 37.129838264031264 40.0 37.0 41.0 30.0 41.0 52-53 36.974588352127114 40.0 37.0 41.0 30.0 41.0 54-55 36.80627122309072 40.0 36.0 41.0 30.0 41.0 56-57 36.60148091123142 40.0 36.0 41.0 30.0 41.0 58-59 36.357363311282676 40.0 35.0 41.0 30.0 41.0 60-61 36.11372072815223 39.0 35.0 41.0 30.0 41.0 62-63 35.815869666196825 39.0 35.0 41.0 30.0 41.0 64-65 35.49317907883777 38.5 35.0 41.0 29.0 41.0 66-67 35.14985103792927 37.5 35.0 40.5 29.0 41.0 68-69 34.79149564726423 37.0 35.0 40.0 28.0 41.0 70-71 34.65318716763839 37.0 35.0 39.5 29.0 41.0 72-73 34.393917081784984 36.0 35.0 39.0 29.0 41.0 74-75 34.04122435209508 36.0 35.0 39.0 28.5 41.0 76-77 33.678607324769345 35.0 35.0 37.5 28.0 40.0 78-79 33.336916565222964 35.0 34.0 37.0 28.0 39.0 80-81 33.01594384690544 35.0 34.0 37.0 27.5 39.0 82-83 32.7143687331817 35.0 34.0 36.0 27.0 38.0 84-85 32.434471204670686 35.0 34.0 36.0 27.0 37.0 86-87 32.22711009578421 35.0 34.0 36.0 26.0 37.0 88-89 32.04033238163122 35.0 34.0 35.0 26.0 36.5 90-91 31.878426728280367 35.0 34.0 35.0 26.0 36.0 92-93 31.72880594086366 35.0 34.0 35.0 25.0 36.0 94-95 31.61117295617632 35.0 34.0 35.0 25.0 36.0 96-97 31.47812570076243 35.0 34.0 35.0 25.0 36.0 98-99 31.38944371476166 35.0 34.0 35.0 24.0 35.0 100-101 31.315866162384673 35.0 33.5 35.0 24.0 35.0 102-103 30.57798785078165 34.5 31.5 35.0 21.5 35.0 104-105 31.105352123109945 35.0 33.0 35.0 23.0 35.0 106-107 31.150693955023065 35.0 33.0 35.0 23.0 35.0 108-109 31.10147140088416 35.0 33.0 35.0 21.5 35.0 110-111 30.99455607701179 35.0 33.0 35.0 20.0 35.0 112-113 30.87915702284085 35.0 33.0 35.0 19.0 35.0 114-115 30.737830259322145 35.0 33.0 35.0 16.0 35.0 116-117 30.646058411551767 35.0 33.0 35.0 11.5 35.0 118-119 30.49399596761276 35.0 33.0 35.0 2.0 35.0 120-121 30.304291068682726 35.0 33.0 35.0 2.0 35.0 122-123 30.042031229978218 35.0 33.0 35.0 2.0 35.0 124-125 28.02458875256279 33.0 28.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1203256771010821 1101 2 0.055723964384956304 1101 3 0.03963499367961276 1101 4 0.02445708072035302 1101 5 -0.00579706704093752 1101 6 0.1093028038634003 1101 7 0.15290214408850034 1101 8 0.14063114149579548 1101 9 0.13953445355119953 1101 10-11 0.13529971493288429 1101 12-13 0.11372466049584773 1101 14-15 0.11891239739492931 1101 16-17 0.1673396586952265 1101 18-19 0.1893058536449317 1101 20-21 0.16400528470905584 1101 22-23 0.1356512146194504 1101 24-25 0.16396918730011123 1101 26-27 0.29178422585994923 1101 28-29 0.36539644654543935 1101 30-31 0.3685572676703899 1101 32-33 0.4130243952560235 1101 34-35 0.37705417544295017 1101 36-37 0.36829155334407915 1101 38-39 0.4670724238232893 1101 40-41 0.48014762776971764 1101 42-43 0.4618903382743298 1101 44-45 0.3034078445887758 1101 46-47 0.34679247009641045 1101 48-49 0.39459732802001923 1101 50-51 0.37474347174043743 1101 52-53 0.2919145324146939 1101 54-55 0.24410086833957223 1101 56-57 0.18977967115991135 1101 58-59 0.1543860775064161 1101 60-61 0.23241757292759502 1101 62-63 0.2275306389311922 1101 64-65 0.2204344758605643 1101 66-67 0.13445195950165356 1101 68-69 0.3026662663481119 1101 70-71 0.3100660554607586 1101 72-73 0.2178190680052694 1101 74-75 0.2464055308164177 1101 76-77 0.2941382338623413 1101 78-79 0.27459516816526985 1101 80-81 0.322335347879644 1101 82-83 0.2563736608410636 1101 84-85 0.30313461551980225 1101 86-87 0.216111795079577 1101 88-89 0.21907118300106276 1101 90-91 0.1868693506991832 1101 92-93 0.17667742022724653 1101 94-95 0.2546818547616745 1101 96-97 0.2273459844283643 1101 98-99 0.1485076193947883 1101 100-101 0.1862062660512933 1101 102-103 0.18251228184071877 1101 104-105 0.11590457201803872 1101 106-107 0.21210348456038375 1101 108-109 0.09899557645953294 1101 110-111 0.18519587735295673 1101 112-113 0.2917585875122022 1101 114-115 0.30996093083436094 1101 116-117 0.3805687276428138 1101 118-119 0.37900912517572394 1101 120-121 0.4294577020874115 1101 122-123 0.25271506587302994 1101 124-125 0.1473581770625536 1102 1 -0.023661051498784502 1102 2 0.23547711634248714 1102 3 0.048893122412192014 1102 4 0.020366835199453703 1102 5 0.03900187124705212 1102 6 0.2250214501805914 1102 7 0.1492817061322924 1102 8 0.19694169225889624 1102 9 0.1437892644337495 1102 10-11 0.2147038290669272 1102 12-13 0.11932651849186726 1102 14-15 0.2304452441102569 1102 16-17 0.17946971841652726 1102 18-19 0.00939211774579718 1102 20-21 -0.06736963234469329 1102 22-23 0.0823413274263487 1102 24-25 -0.03597374567932121 1102 26-27 -0.014510399222999126 1102 28-29 0.15757959186727533 1102 30-31 0.19476430151245694 1102 32-33 0.024817361413951744 1102 34-35 -0.00628490883043753 1102 36-37 -0.03780532787688884 1102 38-39 0.05025490557511603 1102 40-41 0.08514430991968425 1102 42-43 0.05289100184432982 1102 44-45 -0.011068598675990415 1102 46-47 0.1436869624653596 1102 48-49 0.0019072152131229814 1102 50-51 -0.0025226198322272353 1102 52-53 0.18785746539411718 1102 54-55 0.11461181724468616 1102 56-57 0.11620435596415746 1102 58-59 -0.018495143462395447 1102 60-61 0.07901478593355904 1102 62-63 -0.036408312628189776 1102 64-65 0.05007482091696147 1102 66-67 0.13616264297876057 1102 68-69 0.038529570926748136 1102 70-71 0.11004747550057203 1102 72-73 0.017273613459806825 1102 74-75 0.08922835762464842 1102 76-77 0.08970956763805304 1102 78-79 0.15146709915398304 1102 80-81 0.13221723241846917 1102 82-83 -0.03747371805741295 1102 84-85 -0.07314142960296266 1102 86-87 -0.05581123079965522 1102 88-89 -0.014764251637295445 1102 90-91 -0.01495812109909167 1102 92-93 -0.09333452403287623 1102 94-95 0.031714369691997035 1102 96-97 -0.08705613899565634 1102 98-99 0.0067226160769351395 1102 100-101 -0.05326022366336858 1102 102-103 -0.042663564211039784 1102 104-105 -0.1567523622885325 1102 106-107 -0.2650723614913737 1102 108-109 -0.28488232665924684 1102 110-111 -0.33349954401399984 1102 112-113 -0.2515645710810297 1102 114-115 -0.17293621581460883 1102 116-117 -0.05022357494511098 1102 118-119 -0.13602604403462593 1102 120-121 -0.084316684110334 1102 122-123 -0.1343612446777378 1102 124-125 -0.15165044934753524 1103 1 0.1539312592741453 1103 2 0.2073708554961371 1103 3 0.37680982245224115 1103 4 0.3179949222372329 1103 5 0.2556143395423405 1103 6 0.262620702616843 1103 7 0.15067377661687686 1103 8 0.15261116228960248 1103 9 0.33148703509186817 1103 10-11 0.3201095158911187 1103 12-13 0.37391255506909005 1103 14-15 0.46885934102655824 1103 16-17 0.2522984462232287 1103 18-19 0.29364522147026406 1103 20-21 0.2759912006549001 1103 22-23 0.21662166383003267 1103 24-25 0.26292252985131626 1103 26-27 0.24475645433460613 1103 28-29 0.22413145410195767 1103 30-31 0.2985459061046356 1103 32-33 0.32774941320943896 1103 34-35 0.2252524026099536 1103 36-37 0.2439252821885276 1103 38-39 0.05679235050903486 1103 40-41 0.12692244368020056 1103 42-43 0.2390551988807772 1103 44-45 0.16482353855535337 1103 46-47 0.03954732823761731 1103 48-49 0.11928032961706947 1103 50-51 0.12943172538202674 1103 52-53 0.05918732789578485 1103 54-55 0.08211548831663862 1103 56-57 0.06581909364938099 1103 58-59 0.10874634632535418 1103 60-61 0.015153352213097548 1103 62-63 -0.01720820583337712 1103 64-65 0.01778487297944764 1103 66-67 0.05488991570603474 1103 68-69 0.09642597995091506 1103 70-71 0.11516214643903311 1103 72-73 -0.0026655134925306356 1103 74-75 0.07613371071501973 1103 76-77 0.031394522917722156 1103 78-79 0.16944226935823536 1103 80-81 0.06723979282428871 1103 82-83 0.10076737124975921 1103 84-85 0.2368404152775625 1103 86-87 0.21084796022957875 1103 88-89 0.4066174063522965 1103 90-91 0.32403560470520176 1103 92-93 0.33058804972229794 1103 94-95 0.2743797014234097 1103 96-97 0.2189646859943295 1103 98-99 0.25293273488617274 1103 100-101 0.12374391463592005 1103 102-103 0.197260344479389 1103 104-105 0.2842197375646265 1103 106-107 0.3445461140125623 1103 108-109 0.3424841130684264 1103 110-111 0.3283162349207238 1103 112-113 0.4501366704087566 1103 114-115 0.37513987549496264 1103 116-117 0.4286913896791056 1103 118-119 0.5258353896858381 1103 120-121 0.3511197062247362 1103 122-123 0.267963144589384 1103 124-125 0.29480236069117893 1104 1 0.18341587157813777 1104 2 0.16126732613269823 1104 3 0.1982078077268774 1104 4 0.3164227792553973 1104 5 0.21041445865963482 1104 6 0.19766480682394416 1104 7 0.15868030753089357 1104 8 0.16519344051064877 1104 9 0.10750954415402703 1104 10-11 0.2434590738221729 1104 12-13 0.18500484017018692 1104 14-15 0.12436132802633892 1104 16-17 0.28632286526967476 1104 18-19 0.13225925061293253 1104 20-21 0.23205694108187913 1104 22-23 0.29466300574802773 1104 24-25 0.20728499557942115 1104 26-27 0.2139091811965841 1104 28-29 0.15806910235678373 1104 30-31 0.14157549032365324 1104 32-33 0.1277684103650003 1104 34-35 0.15461019606466664 1104 36-37 0.26682404275248217 1104 38-39 0.1468003601205652 1104 40-41 0.19514430991968368 1104 42-43 0.271688204641535 1104 44-45 0.3115467859393988 1104 46-47 0.1462743750527693 1104 48-49 0.31285826416416995 1104 50-51 0.22202283471322914 1104 52-53 0.14335396889062224 1104 54-55 0.25349293612580226 1104 56-57 0.28593862169842055 1104 58-59 0.21100136003411052 1104 60-61 0.2735742264930039 1104 62-63 0.1691441349242524 1104 64-65 0.20859230343445034 1104 66-67 0.3083864192025416 1104 68-69 0.3584316688288496 1104 70-71 0.2706908321439343 1104 72-73 0.23600088618708526 1104 74-75 0.1831724135686983 1104 76-77 -0.06640232047384131 1104 78-79 0.08885171453859897 1104 80-81 0.0034899596911941444 1104 82-83 0.18086194627825591 1104 84-85 0.19959982913830032 1104 86-87 0.3051118461234168 1104 88-89 0.25138959451655296 1104 90-91 0.2888600607190881 1104 92-93 0.23772841303005876 1104 94-95 0.19316891514654344 1104 96-97 0.28179700785748807 1104 98-99 0.3586806580349773 1104 100-101 0.35669781829466984 1104 102-103 0.44167210012462377 1104 104-105 0.3415413440051758 1104 106-107 0.25098358256456876 1104 108-109 0.2755792118022953 1104 110-111 0.3771717846573317 1104 112-113 0.3541137505972891 1104 114-115 0.4303784695000772 1104 116-117 0.47876943204789413 1104 118-119 0.48209983009124713 1104 120-121 0.49499800120435467 1104 122-123 0.5280583357418429 1104 124-125 0.47592997023288675 1105 1 -0.14316050770759858 1105 2 -0.18633236481674942 1105 3 -0.1696223916304156 1105 4 -0.14230273168686125 1105 5 -0.2653993826714469 1105 6 -0.00388930408976762 1105 7 -0.058059602805073496 1105 8 -0.04068098063471126 1105 9 -0.0961631378437744 1105 10-11 -0.10865334577793107 1105 12-13 -0.10466657216132091 1105 14-15 -0.15114518516489284 1105 16-17 -0.19133445278173156 1105 18-19 -0.24587370018011967 1105 20-21 -0.13967748213038078 1105 22-23 -0.04638338628358696 1105 24-25 -0.08515089365449313 1105 26-27 0.02754628301102713 1105 28-29 0.007950009626860322 1105 30-31 -0.16208759535914652 1105 32-33 0.035681925421378935 1105 34-35 0.07013604952155106 1105 36-37 -0.08062382957223946 1105 38-39 -0.06865290582531003 1105 40-41 -0.01930454172128293 1105 42-43 0.015644248597581623 1105 44-45 0.027663920036822276 1105 46-47 -0.09722916574141038 1105 48-49 -0.1484560372979189 1105 50-51 -0.06259427231767489 1105 52-53 -0.1106895245617352 1105 54-55 -0.2534951496800133 1105 56-57 -0.2465284186503851 1105 58-59 -0.30702076562348424 1105 60-61 -0.2772052895574504 1105 62-63 -0.35321798571373364 1105 64-65 -0.31559237887830704 1105 66-67 -0.5649200772566658 1105 68-69 -0.546395769742432 1105 70-71 -0.297084249916864 1105 72-73 -0.5180359636625766 1105 74-75 -0.38027276194543447 1105 76-77 -0.3419715788017186 1105 78-79 -0.23150699079547365 1105 80-81 -0.2061703307572742 1105 82-83 -0.25869283391691766 1105 84-85 -0.2901599185032211 1105 86-87 -0.22120026541036708 1105 88-89 -0.38370289919663136 1105 90-91 -0.21195562605718976 1105 92-93 -0.24318008733656526 1105 94-95 -0.2613061664221341 1105 96-97 -0.08355463557297327 1105 98-99 -0.014852459094200299 1105 100-101 -0.1003648234970349 1105 102-103 -0.08173208190024539 1105 104-105 -0.03878473385784886 1105 106-107 0.03821802300097943 1105 108-109 0.007800930969821707 1105 110-111 -0.010302563654459362 1105 112-113 -0.03892916707616578 1105 114-115 -0.03341168123588645 1105 116-117 0.014304094429849812 1105 118-119 -0.03736543370898815 1105 120-121 0.08801803535739339 1105 122-123 0.05592037518023929 1105 124-125 0.048064732022819356 1106 1 -0.022812397359626146 1106 2 -0.09106986181901533 1106 3 -0.08993826407841254 1106 4 -0.0348576442317281 1106 5 -0.0027091495757005646 1106 6 -0.14530409578182457 1106 7 -0.08643337766658732 1106 8 0.012345450511816125 1106 9 -0.13326964872013747 1106 10-11 -0.09882029485384436 1106 12-13 -0.05861355261021828 1106 14-15 -0.08176145980035443 1106 16-17 0.01874854015344596 1106 18-19 0.052972615460369354 1106 20-21 0.15077739660400624 1106 22-23 0.17724787897535776 1106 24-25 0.055291109577154884 1106 26-27 0.04721484201620996 1106 28-29 0.04460219217200034 1106 30-31 0.11061727256375065 1106 32-33 0.07215154120064682 1106 34-35 0.15985348629398288 1106 36-37 0.12125815612514401 1106 38-39 0.02151468211143026 1106 40-41 0.10067376649662663 1106 42-43 0.10941918874123502 1106 44-45 0.042637343426598306 1106 46-47 0.035864463735038044 1106 48-49 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-0.6890303207981958 2216 58-59 -0.6328099946628569 2216 60-61 -0.6591474798449397 2216 62-63 -0.7953225791734582 2216 64-65 -0.8284179188156244 2216 66-67 -0.6025955877204225 2216 68-69 -0.6417251853870454 2216 70-71 -0.5289917730737344 2216 72-73 -0.5028187812303742 2216 74-75 -0.4716238971316642 2216 76-77 -0.4970712825081165 2216 78-79 -0.5379696191092975 2216 80-81 -0.5329982753210203 2216 82-83 -0.5297261740884665 2216 84-85 -0.5078331437928298 2216 86-87 -0.6182817344657323 2216 88-89 -0.45125056974723066 2216 90-91 -0.5350844449597361 2216 92-93 -0.6092377204674371 2216 94-95 -0.5185193965417696 2216 96-97 -0.5211135502191198 2216 98-99 -0.5385728264878793 2216 100-101 -0.5689761387445991 2216 102-103 -0.586021977015946 2216 104-105 -0.6228440860629121 2216 106-107 -0.7963886024459939 2216 108-109 -0.7467635745626353 2216 110-111 -0.662001772412907 2216 112-113 -0.5488891505605906 2216 114-115 -0.5729958314757013 2216 116-117 -0.5847841730186225 2216 118-119 -0.6394789601222293 2216 120-121 -0.6779502682383836 2216 122-123 -0.791214457885193 2216 124-125 -0.943219146333206 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13320.0 3 2372.0 4 1890.0 5 1699.0 6 2678.0 7 2861.0 8 2701.0 9 2827.0 10 3446.0 11 4016.0 12 4445.0 13 4344.0 14 3961.0 15 3606.0 16 3232.0 17 2890.0 18 2604.0 19 2362.0 20 2151.0 21 2184.0 22 2472.0 23 3485.0 24 5606.0 25 7433.0 26 9905.0 27 8262.0 28 8913.0 29 11713.0 30 12680.0 31 14150.0 32 19354.0 33 26408.0 34 38958.0 35 65243.0 36 141232.0 37 347360.0 38 205378.0 39 771.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.02443176128968 20.563632722539303 13.50455592269951 21.907379593471514 2 14.78068023669113 22.59809565765892 40.22187290367152 22.399351201978433 3 17.429354660429798 30.292993936675504 29.64751419715016 22.63013720574454 4 11.587768783417975 18.416527545073894 38.74336898251124 31.252334688996893 5 12.041170709431649 41.917374175574366 33.44939884274976 12.59205627224422 6 28.51897282872635 38.91785533687473 19.09429087832256 13.468880956076362 7 27.176003564223606 35.16156645625066 22.30673347917282 15.35569650035292 8 26.289065393802396 35.542125253528376 20.99285014946411 17.175959203205117 9 28.351406742311003 17.08295078980013 20.0279936763927 34.53764879149617 10-11 23.52442986633938 29.382962737902947 27.654629719078937 19.43797767667874 12-13 24.65295923209534 27.166289241686137 27.828534930331557 20.35221659588697 14-15 22.614566442504138 27.851589154291673 25.57099867885451 23.962845724349677 16-17 21.22552992120821 31.177091566360343 26.37975730266889 21.21762120976255 18-19 21.538594002328747 29.944104098732403 27.957261611439886 20.560040287498964 20-21 23.408205486041624 28.768756623039494 27.303316062331778 20.519721828587105 22-23 23.12336603065643 28.70280040384039 27.032729191182987 21.141104374320197 24-25 22.159516118455468 29.206644310722595 27.303986276073385 21.329853294748556 26-27 21.64909080716142 29.297242588907086 27.325289215823677 21.728377388107813 28-29 22.010073544281745 28.806462205687716 27.567392231497294 21.616072018533238 30-31 22.012514709864657 28.967934372104644 27.229830279070722 21.78972063895998 32-33 21.594815094010958 29.36236328079051 27.096469664866184 21.946351960332347 34-35 22.15891998440302 29.01988814824937 27.361032483858683 21.46015938348893 36-37 22.281730229661754 28.671752240227022 26.828411199608343 22.21810633050288 38-39 21.96028553251534 28.96550274275194 27.09986079764302 21.9743509270897 40-41 21.764911720372673 28.67539759498151 27.63052964772752 21.929161036918302 42-43 21.634344399092154 28.770222066042145 27.157662785191327 22.437770749674375 44-45 21.739935669171757 28.942615820017842 27.112100422565245 22.20534808824516 46-47 22.097022796707872 28.744206210111766 27.078768510216943 22.08000248296342 48-49 21.48169505337462 29.082685359546613 26.944346835415157 22.491272751663608 50-51 21.942964641241307 28.99083817989045 26.997433969518003 22.06876320935024 52-53 21.86342592013231 28.80090342584004 27.138515784673494 22.19715486935415 54-55 21.730183480038946 28.955057276506498 27.075606990812233 22.239152252642327 56-57 21.69280597508047 29.12190946271331 26.82368606799057 22.361598494215645 58-59 21.59584597839484 28.39649439345257 27.280391452055238 22.727268176097354 60-61 21.67489410777731 29.197321434828353 26.949933576469153 22.177850880925185 62-63 21.610614286036085 29.0943767410778 27.301251198261987 21.99375777462413 64-65 21.503103936057215 28.97327341366893 26.993431728923916 22.530190921349934 66-67 21.547599323452754 29.435311301332323 26.57294982307959 22.444139552135333 68-69 21.67964829717147 29.015533408114734 27.164268306051138 22.14054998866266 70-71 21.872766190053802 28.789542816067215 27.08378802107215 22.253902972806834 72-73 21.757748582577886 29.136602601739796 26.593727053078435 22.511921762603883 74-75 21.54707526887103 29.50981250985506 26.691128069459708 22.25198415181421 76-77 21.588920866838844 29.236562461046166 27.18575740266736 21.98875926944763 78-79 21.72603986310853 28.84266440816764 27.021649455122095 22.40964627360174 80-81 21.64850044174586 29.526994050822047 26.697217626786067 22.127287880646023 82-83 22.12401960759777 28.975766807138065 26.688397269592457 22.21181631567171 84-85 21.77193786840082 28.884226037929267 26.92614564646335 22.41769044720656 86-87 21.733759271513566 29.270452390796613 26.915895896757714 22.07989244093211 88-89 22.070343981106518 28.785629260469154 27.051006994953262 22.093019763471066 90-91 21.873352812176662 28.836129933929776 26.966301133840876 22.324216120052682 92-93 22.003769646088806 28.933009934744575 26.815800474084313 22.247419945082314 94-95 21.787204478472578 28.394693426447976 27.17877050230651 22.639331592772937 96-97 22.08152406504106 28.45668893265893 26.998445802038727 22.463341200261286 98-99 21.58793816915135 28.723433543250408 26.918974277858393 22.769654009739845 100-101 21.468057246283955 28.877218413634033 27.089923837134805 22.564800502947207 102-103 21.760175070476677 28.668090882880577 26.893259866735008 22.67847417990774 104-105 21.151898664474746 29.225471443567685 27.04364798279571 22.578981909161858 106-107 21.312588095848284 29.070578789723218 26.834796258329064 22.782036856099435 108-109 21.516910398513584 29.292320044208097 26.361981836237824 22.82878772104049 110-111 21.268114146591007 29.49589476914052 26.619304971376256 22.61668611289222 112-113 21.025057791402322 29.728407940723894 27.021898160986975 22.224636106886813 114-115 21.13877460920352 30.117145089622028 26.32703617367086 22.417044127503594 116-117 20.87764839204411 30.242925274399646 26.530921766569094 22.348504566987152 118-119 21.08884911028751 30.011102634798405 26.39668943257427 22.503358822339823 120-121 20.955397625171067 30.190721128289017 26.42019715825419 22.433684088285734 122-123 21.060774653210018 30.17554608191016 26.424162701100144 22.339516563779675 124-125 21.33259329315209 30.477967106950498 25.865957216593998 22.32348238330341 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1767.0 1 1550.5 2 1511.5 3 1474.0 4 1016.0 5 677.5 6 540.5 7 518.0 8 556.5 9 559.5 10 473.5 11 372.5 12 331.5 13 326.5 14 353.5 15 382.5 16 420.5 17 505.0 18 604.0 19 711.5 20 889.0 21 1181.0 22 1628.0 23 2233.5 24 2713.0 25 3540.0 26 4730.0 27 5996.0 28 7383.5 29 9215.5 30 12186.5 31 15258.0 32 19127.5 33 22859.5 34 26096.5 35 29588.0 36 35151.5 37 39879.5 38 39769.0 39 39229.0 40 37387.5 41 35652.0 42 34509.5 43 34809.0 44 36482.5 45 36002.5 46 34517.0 47 32572.5 48 30516.0 49 29144.5 50 28594.5 51 27546.5 52 26121.0 53 25983.0 54 26590.0 55 25447.5 56 23777.5 57 22302.5 58 21436.0 59 20279.0 60 17568.5 61 14839.0 62 12274.5 63 8745.0 64 5709.0 65 4443.5 66 3381.5 67 2551.0 68 1920.0 69 1280.0 70 905.5 71 658.5 72 527.0 73 597.0 74 478.0 75 289.5 76 198.5 77 116.0 78 95.0 79 57.5 80 31.5 81 25.0 82 18.5 83 8.0 84 8.5 85 10.0 86 5.5 87 6.0 88 6.0 89 5.5 90 4.0 91 1.0 92 5.5 93 8.5 94 5.0 95 4.5 96 4.5 97 3.0 98 3.0 99 4.0 100 9.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021223090722706305 2 0.014215466427473091 3 0.012013070220399796 4 0.03854193362378268 5 0.03383681445412609 6 0.024726902870322913 7 0.009710565094823168 8 0.001001089185033316 9 0.011512525627883137 10-11 1.5016337775499745E-4 12-13 0.003804138903126602 14-15 0.01576715466427473 16-17 0.0013514703997949769 18-19 0.009109911583803178 20-21 0.003153430932854946 22-23 2.50272296258329E-4 24-25 0.007257896591491543 26-27 4.0043567401332647E-4 28-29 0.005806317273193235 30-31 7.508168887749873E-4 32-33 0.0012513614812916453 34-35 5.005445925166581E-5 36-37 0.007358005509994874 38-39 6.507079702716555E-4 40-41 0.01256366927216812 42-43 0.006507079702716555 44-45 0.001301415940543311 46-47 0.010561490902101487 48-49 0.0029031586365966173 50-51 0.00931012942080984 52-53 0.0047551736289082516 54-55 0.002953213095848283 56-57 0.009710565094823168 58-59 0.016918407227063045 60-61 0.001902069451563301 62-63 0.006957569835981547 64-65 0.001902069451563301 66-67 0.001651797155304972 68-69 5.005445925166581E-5 70-71 0.005806317273193235 72-73 3.503812147616607E-4 74-75 0.005956480650948231 76-77 0.013364540620194773 78-79 0.001852014992311635 80-81 0.003453757688364941 82-83 0.0013514703997949769 84-85 0.0 86-87 0.001902069451563301 88-89 0.00490533700666325 90-91 0.005055500384418247 92-93 0.01446573872373142 94-95 0.0 96-97 4.504901332649923E-4 98-99 0.0025527774218349563 100-101 0.0 102-103 0.0 104-105 0.009159966043054845 106-107 0.0 108-109 0.0 110-111 3.503812147616607E-4 112-113 0.0061066440287032295 114-115 0.0022524506663249614 116-117 4.0043567401332647E-4 118-119 0.005005445925166581 120-121 0.001902069451563301 122-123 5.005445925166581E-5 124-125 0.0768836494105587 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 998912.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.63569066265417 #Duplication Level Percentage of deduplicated Percentage of total 1 65.02889458858544 21.87291782516006 2 13.37098311624387 8.994845039071011 3 6.360119945438397 6.417810811864285 4 3.7286923399506318 5.016685684911504 5 2.441942398356101 4.106820956356283 6 1.7011995919908187 3.4332613938982 7 1.2038366059711896 2.8344312980778503 8 0.9348945848855695 2.5156660047521204 9 0.7069443512494299 2.1400705362902905 >10 4.043561724855187 24.58899843741007 >50 0.31412246656532133 7.287728928529449 >100 0.1588477692400434 9.408110730636928 >500 0.005662490834666316 1.1540982049279824 >1k 2.980258334034903E-4 0.22855414811394076 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2280 0.22824833418759613 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.5016337775499745E-4 0.0 20-21 0.0 0.0 0.0 4.0043567401332647E-4 0.0 22-23 0.0 0.0 0.0 0.0013514703997949769 0.0 24-25 0.0 0.0 0.0 0.003003267555099949 0.0 26-27 0.0 0.0 0.0 0.005255718221424911 0.0 28-29 0.0 0.0 0.0 0.010060946309584828 0.0 30-31 0.0 0.0 0.0 0.027029407995899535 0.0 32-33 0.0 0.0 0.0 0.05631126665812404 0.0 34-35 0.0 0.0 0.0 0.09330151204510508 0.0 36-37 0.0 0.0 0.0 0.14741038249615582 0.0 38-39 0.0 0.0 0.0 0.2412624935930292 0.0 40-41 0.0 0.0 0.0 0.35919079958995387 0.0 42-43 0.0 0.0 0.0 0.4642050550999487 0.0 44-45 0.0 0.0 0.0 0.5833346681189133 0.0 46-47 0.0 0.0 0.0 0.7233870451050743 0.0 48-49 0.0 0.0 0.0 0.8807082105330599 0.0 50-51 0.0 0.0 0.0 1.0606039370835467 0.0 52-53 0.0 0.0 0.0 1.235243945412609 0.0 54-55 0.0 0.0 0.0 1.4112854786007176 0.0 56-57 0.0 0.0 0.0 1.6031942753716044 0.0 58-59 0.0 0.0 0.0 1.8222826435161457 0.0 60-61 0.0 0.0 0.0 2.081014143388006 0.0 62-63 0.0 0.0 0.0 2.3331384546386467 0.0 64-65 0.0 0.0 0.0 2.579656666453101 0.0 66-67 0.0 0.0 0.0 2.849199929523321 0.0 68-69 0.0 0.0 0.0 3.1591871956688875 0.0 70-71 0.0 0.0 0.0 3.512071133393132 0.0 72-73 0.0 0.0 0.0 3.8851270181957966 0.0 74-75 0.0 0.0 0.0 4.250624679651461 0.0 76-77 0.0 0.0 0.0 4.611767603152229 0.0 78-79 0.0 0.0 0.0 5.037530833546899 0.0 80-81 0.0 0.0 0.0 5.5306173116350585 0.0 82-83 0.0 0.0 0.0 6.061645069835981 0.0 84-85 0.0 0.0 0.0 6.589018852511533 0.0 86-87 0.0 0.0 0.0 7.123700586237827 0.0 88-89 0.0 0.0 0.0 7.70012773898001 0.0 90-91 0.0 0.0 0.0 8.344929283059969 0.0 92-93 0.0 0.0 0.0 9.05735440158893 0.0 94-95 0.0 0.0 0.0 9.772732733213736 0.0 96-97 0.0 0.0 0.0 10.488010955920041 0.0 98-99 0.0 0.0 0.0 11.226614556637621 0.0 100-101 0.0 1.0010891850333163E-4 0.0 12.059771030881599 0.0 102-103 0.0 1.0010891850333163E-4 0.0 12.972614204254228 0.0 104-105 0.0 1.0010891850333163E-4 0.0 13.883805580471552 0.0 106-107 0.0 1.0010891850333163E-4 0.0 14.77522544528447 0.0 108-109 0.0 1.0010891850333163E-4 0.0 15.665994602127114 0.0 110-111 0.0 1.0010891850333163E-4 0.0 16.653318810866224 0.0 112-113 0.0 1.0010891850333163E-4 0.0 17.711319916068682 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3405 0.0 59.793488 1 GGTATCA 2370 0.0 54.5075 1 TCAACGC 4410 0.0 45.087143 4 ATCAACG 4410 0.0 44.952152 3 CAACGCA 4575 0.0 43.588993 5 AACGCAG 4720 0.0 41.97876 6 TATCAAC 4935 0.0 41.014423 2 GTACATG 9245 0.0 40.05238 1 AGGTAAG 260 0.0 38.924267 2 TACATGG 9990 0.0 36.707947 2 ACATGGG 9920 0.0 34.806564 3 ACGCAGA 5805 0.0 34.02499 7 CGCAGAG 5835 0.0 33.751472 8 CTCCCGC 215 3.6379788E-12 33.205112 7 CATGGGA 7175 0.0 33.188145 4 ATGGGAG 2815 0.0 31.720201 5 GTAAGGT 415 0.0 30.124239 4 CATGGGG 2550 0.0 30.115799 4 TAAGGTG 455 0.0 30.091204 5 GGAACGA 140 3.0944993E-6 29.749226 8 >>END_MODULE