FastQCFastQC Report
Fri 27 May 2016
SRR940104_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940104_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998912
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73320.7339985904664275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55610.5567056957970271No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35570.35608742311635055No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT11120.11132111737570476No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11110.11122100845720143No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10350.10361273065094823No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36800.074.306961
GTATCAA68800.066.156051
ATCAACG91350.049.5494843
TCAACGC92750.048.8015674
TATCAAC92800.048.7752722
CAACGCA95450.047.3807565
AACGCAG96600.046.812016
ACGCAGA112650.040.2439967
CGCAGAG113550.039.8202328
GTACATG79700.038.122071
TCGACCG401.19843084E-437.18410518-19
GCAGAGT120200.037.0727429
ACATGGG79800.035.823863
TACATGG85650.035.0459022
ATGGGAG21950.034.971925
CATGGGA59000.034.321084
GTGGTAT11000.034.1202771
ATCCGCG452.3942016E-433.0541952-53
GTCCGTA750.003891900431.7638262
GAGTACT74000.030.7924612-13
Produced by FastQC (version 0.11.5)