##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940104_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 998912 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.79748866767043 34.0 31.0 34.0 31.0 34.0 2 32.8591007015633 34.0 31.0 34.0 31.0 34.0 3 32.91926516049462 34.0 31.0 34.0 31.0 34.0 4 36.32467524666838 37.0 37.0 37.0 35.0 37.0 5 36.309627875128136 37.0 37.0 37.0 35.0 37.0 6 36.36595716139159 37.0 37.0 37.0 35.0 37.0 7 36.32814101742696 37.0 37.0 37.0 35.0 37.0 8 36.333577932790874 37.0 37.0 37.0 35.0 37.0 9 38.154160726870835 39.0 39.0 39.0 37.0 39.0 10-11 38.12618729177345 39.0 39.0 39.0 36.0 39.0 12-13 38.12958749119042 39.0 39.0 39.0 37.0 39.0 14-15 39.60336395998847 41.0 39.5 41.0 37.0 41.0 16-17 39.603542654408 41.0 39.5 41.0 37.0 41.0 18-19 39.67818536567786 41.0 40.0 41.0 37.0 41.0 20-21 39.664141586045616 41.0 40.0 41.0 37.0 41.0 22-23 39.608714781682465 41.0 40.0 41.0 36.5 41.0 24-25 39.57247685481804 41.0 40.0 41.0 36.0 41.0 26-27 39.43921586686315 41.0 39.0 41.0 36.0 41.0 28-29 39.32414567048949 41.0 39.0 41.0 35.5 41.0 30-31 39.104972710308814 41.0 39.0 41.0 35.0 41.0 32-33 38.842579226198104 41.0 38.0 41.0 35.0 41.0 34-35 38.67419101982958 41.0 38.0 41.0 35.0 41.0 36-37 38.51658654616223 41.0 38.0 41.0 35.0 41.0 38-39 38.32025343573808 40.0 38.0 41.0 35.0 41.0 40-41 38.165422980202464 40.0 38.0 41.0 34.0 41.0 42-43 37.92713672475654 40.0 38.0 41.0 33.5 41.0 44-45 37.85612946886212 40.5 38.0 41.0 33.5 41.0 46-47 37.44969827171963 41.0 38.0 41.0 33.0 41.0 48-49 37.03565729513711 40.5 37.5 41.0 31.5 41.0 50-51 36.7995704326307 40.0 37.0 41.0 31.0 41.0 52-53 36.66096062516017 40.0 37.0 41.0 31.0 41.0 54-55 36.486353152229626 40.0 36.5 41.0 30.5 41.0 56-57 36.30728682806894 40.0 36.0 41.0 30.0 41.0 58-59 36.092705363435414 40.0 35.0 41.0 30.0 41.0 60-61 35.85017198712199 39.0 35.0 41.0 29.0 41.0 62-63 35.59071219486802 39.0 35.0 41.0 29.0 41.0 64-65 35.29329710725269 38.5 35.0 41.0 28.5 41.0 66-67 34.97871934664916 37.5 35.0 40.5 28.0 41.0 68-69 34.622356123462325 37.0 35.0 40.0 28.0 41.0 70-71 34.34002544768708 37.0 35.0 39.5 27.5 41.0 72-73 34.133043251057146 36.0 35.0 39.0 28.0 41.0 74-75 33.81846549045361 36.0 35.0 39.0 28.0 41.0 76-77 33.48666649314454 35.0 35.0 37.5 27.5 40.0 78-79 33.165220259642496 35.0 35.0 37.0 27.0 39.0 80-81 32.87372811619042 35.0 34.5 37.0 27.0 39.0 82-83 32.596255225685546 35.0 34.0 36.0 26.0 38.0 84-85 32.35681221168632 35.0 34.0 36.0 26.0 37.0 86-87 32.17079232204639 35.0 34.0 35.5 26.0 37.0 88-89 32.002208402742184 35.0 34.0 35.0 25.5 36.5 90-91 31.874701675422862 35.0 34.0 35.0 25.0 36.0 92-93 31.748799694067145 35.0 34.0 35.0 25.0 36.0 94-95 31.644663393772426 35.0 34.0 35.0 25.0 36.0 96-97 31.568558591747824 35.0 34.0 35.0 24.5 36.0 98-99 31.51113211173757 35.0 34.0 35.0 24.0 35.0 100-101 31.47303015681061 35.0 34.0 35.0 24.0 35.0 102-103 30.73755546034085 34.5 32.5 35.0 21.5 35.0 104-105 31.313800414851357 35.0 34.0 35.0 23.5 35.0 106-107 31.359779440030753 35.0 34.0 35.0 23.0 35.0 108-109 31.359060157451307 35.0 34.0 35.0 23.0 35.0 110-111 31.301965538505893 35.0 34.0 35.0 21.5 35.0 112-113 31.237703621540234 35.0 34.0 35.0 20.0 35.0 114-115 31.153402401813175 35.0 34.0 35.0 19.0 35.0 116-117 31.058644305003845 35.0 34.0 35.0 17.5 35.0 118-119 30.967396026877243 35.0 34.0 35.0 11.0 35.0 120-121 30.838897220175554 35.0 34.0 35.0 2.0 35.0 122-123 30.66180053898642 35.0 34.0 35.0 2.0 35.0 124-125 28.983444988147106 33.5 29.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19087607383724503 1101 2 0.1754052176141272 1101 3 0.15160444498497583 1101 4 0.1209848795049453 1101 5 0.08716233485026237 1101 6 0.09287687338111539 1101 7 0.12149423331061371 1101 8 0.09826890172255531 1101 9 0.17600548596522714 1101 10-11 0.14020121335175162 1101 12-13 0.19291645279470515 1101 14-15 0.16674893487083153 1101 16-17 0.20052903811448175 1101 18-19 0.20043153511147693 1101 20-21 0.21300541325345534 1101 22-23 0.24231739733004076 1101 24-25 0.1441936321751669 1101 26-27 0.08484434948867658 1101 28-29 0.15894175054639703 1101 30-31 0.14297548362503676 1101 32-33 0.25286584044646077 1101 34-35 0.3202851013324448 1101 36-37 0.21727294107507333 1101 38-39 0.1825093926405117 1101 40-41 0.15145380076550907 1101 42-43 0.17477632448817104 1101 44-45 0.07183158667522349 1101 46-47 0.09856888242681805 1101 48-49 0.17425241263766367 1101 50-51 0.1328003295184388 1101 52-53 0.16383807072681122 1101 54-55 0.18503692915807335 1101 56-57 0.09818319232538641 1101 58-59 0.18458281575733082 1101 60-61 0.13755160990963589 1101 62-63 0.1828942646897005 1101 64-65 0.039296068436186715 1101 66-67 -0.004218357630506375 1101 68-69 0.047486309001016025 1101 70-71 -0.05041596634283252 1101 72-73 -0.050896243318106826 1101 74-75 -0.10593046406994233 1101 76-77 0.06642719049938961 1101 78-79 0.02306703191604953 1101 80-81 -0.045689839339999594 1101 82-83 0.033688814161699554 1101 84-85 -0.04124771372905656 1101 86-87 -0.06511520415136118 1101 88-89 -0.0028711652875728078 1101 90-91 0.03375645710179853 1101 92-93 0.04491843667511475 1101 94-95 0.074274664834725 1101 96-97 0.07199696676568124 1101 98-99 0.12996346401152437 1101 100-101 0.015733809286931688 1101 102-103 -0.05689612174685266 1101 104-105 -0.031011061769628867 1101 106-107 0.014231872892707997 1101 108-109 0.007793828670568104 1101 110-111 -0.05617742614856169 1101 112-113 -0.08476842239135962 1101 114-115 0.010844478253591916 1101 116-117 -0.005170078152740842 1101 118-119 0.07448343426625215 1101 120-121 -0.04915843283698251 1101 122-123 -0.13887005146424158 1101 124-125 -0.14764486471641902 1102 1 0.09050182035350218 1102 2 0.14689028728897568 1102 3 0.1426117442550492 1102 4 0.15571347937223123 1102 5 -0.005217227193533347 1102 6 0.07881449780049365 1102 7 0.1452633109483017 1102 8 0.1810426243502974 1102 9 0.09985684628373548 1102 10-11 0.16318860552162562 1102 12-13 0.19749906726053723 1102 14-15 0.140978530093129 1102 16-17 0.20082499033743773 1102 18-19 0.18336186026078138 1102 20-21 0.1832296999157066 1102 22-23 0.2580453336273223 1102 24-25 0.2418830814120625 1102 26-27 0.22325310861276648 1102 28-29 0.17711958730817656 1102 30-31 0.2031002347862767 1102 32-33 0.08877028636550222 1102 34-35 0.12969186974651592 1102 36-37 0.08833863450572466 1102 38-39 0.07510395136645798 1102 40-41 0.060660171037575594 1102 42-43 0.1156628540170388 1102 44-45 0.21260265502294118 1102 46-47 -0.12764478711532234 1102 48-49 0.08814358715658699 1102 50-51 -0.03954605402502409 1102 52-53 0.22567217822515317 1102 54-55 0.24708736047857371 1102 56-57 0.2931374059949263 1102 58-59 0.20038374475533516 1102 60-61 0.191531702809435 1102 62-63 0.10966798731742955 1102 64-65 0.24546328807785756 1102 66-67 0.18180818516975705 1102 68-69 0.15204901636664658 1102 70-71 0.1463909347852379 1102 72-73 0.10890999423995851 1102 74-75 0.14214252863078514 1102 76-77 0.19653468884045822 1102 78-79 0.21817784810716745 1102 80-81 0.1783154692200526 1102 82-83 0.123552118740335 1102 84-85 0.0817303884607341 1102 86-87 0.07356429153542621 1102 88-89 -0.0016024194348887022 1102 90-91 0.22589580680319088 1102 92-93 0.08183416328427029 1102 94-95 0.13037818175575921 1102 96-97 0.115651910362228 1102 98-99 0.12598469825173808 1102 100-101 0.05663361021592905 1102 102-103 -0.10940308923192532 1102 104-105 0.08228820830336403 1102 106-107 -0.01976016426721472 1102 108-109 -0.042904246976409155 1102 110-111 0.007749581150708451 1102 112-113 0.02533244024965242 1102 114-115 0.004876329613910713 1102 116-117 -0.07036848558472286 1102 118-119 -0.029605484114636482 1102 120-121 -0.0632433697978314 1102 122-123 -0.14835512113909388 1102 124-125 -0.14340597951402856 1103 1 -0.02056052112293827 1103 2 0.035414649857393954 1103 3 -0.044002058975493696 1103 4 -0.05405397490623898 1103 5 0.01538189174920035 1103 6 0.05230214964937829 1103 7 0.041250495569848056 1103 8 0.1162555465235684 1103 9 0.10605468368862603 1103 10-11 -0.04832467708416743 1103 12-13 0.011358632071640784 1103 14-15 0.052428002793703854 1103 16-17 0.1825766923798895 1103 18-19 0.1282239614488745 1103 20-21 0.15361229235996632 1103 22-23 0.1751357087441221 1103 24-25 0.16172902988355276 1103 26-27 0.16325898232788916 1103 28-29 0.2966133798592381 1103 30-31 0.21318513666854244 1103 32-33 0.12108186025416501 1103 34-35 -0.008565772757819445 1103 36-37 0.003290176355946528 1103 38-39 0.09278249895690038 1103 40-41 0.10772838622913383 1103 42-43 5.836923563791174E-4 1103 44-45 -0.09086764266762515 1103 46-47 -0.20475705517031884 1103 48-49 -0.15706960200374454 1103 50-51 -0.0540851008812524 1103 52-53 -0.03538001774334276 1103 54-55 -0.06467742392938192 1103 56-57 0.04377844190468494 1103 58-59 0.007151065540284662 1103 60-61 0.09859698114343018 1103 62-63 0.05418487424841345 1103 64-65 0.09343124963172045 1103 66-67 0.08927768189916918 1103 68-69 0.19099735437225718 1103 70-71 0.16876525063091918 1103 72-73 0.10198648603009985 1103 74-75 0.10019245520934561 1103 76-77 0.07337543106446276 1103 78-79 -0.04781681215181521 1103 80-81 -0.05215950074390463 1103 82-83 0.038094636428226636 1103 84-85 0.018259022821958126 1103 86-87 -0.014964609812857077 1103 88-89 0.01174613216035425 1103 90-91 -0.030712389699278475 1103 92-93 -0.13769140072587405 1103 94-95 -0.04380737424477843 1103 96-97 0.0955352370208864 1103 98-99 0.15202901810222258 1103 100-101 0.016042500884729805 1103 102-103 0.03885668916882423 1103 104-105 -0.04258110153510586 1103 106-107 -0.06830668676087015 1103 108-109 -0.08909621066617035 1103 110-111 -0.12808595482579577 1103 112-113 -0.08383002137096085 1103 114-115 -0.07364162526527807 1103 116-117 -0.05103168141972603 1103 118-119 -0.19901464177048922 1103 120-121 -0.0921196212996307 1103 122-123 0.02439511247457915 1103 124-125 -0.015137924514696977 1104 1 0.08376056161224454 1104 2 -0.00955726515857691 1104 3 0.09329705894036522 1104 4 -0.05032847866972645 1104 5 -0.07525918523548825 1104 6 0.025024288010278894 1104 7 -0.12242899674400576 1104 8 0.09041325372092501 1104 9 0.06939530782219805 1104 10-11 0.18341238174539853 1104 12-13 0.07621235397381554 1104 14-15 0.11380370491830405 1104 16-17 0.1319158994283498 1104 18-19 0.1608164057153303 1104 20-21 0.040768161454167284 1104 22-23 0.038478900060880505 1104 24-25 0.06718378071276021 1104 26-27 0.11473562609528187 1104 28-29 0.19490979709838996 1104 30-31 0.04406526975131442 1104 32-33 0.1402248318200492 1104 34-35 0.20399956205420722 1104 36-37 0.131667305834398 1104 38-39 0.2624466087091193 1104 40-41 0.23145737383477893 1104 42-43 0.07066984702402834 1104 44-45 0.08946978789007431 1104 46-47 0.037711856241323005 1104 48-49 -0.013017252004253521 1104 50-51 0.04558681310784607 1104 52-53 0.17486098941396477 1104 54-55 0.17547896887018055 1104 56-57 0.007794748652266037 1104 58-59 0.11235577272736208 1104 60-61 -0.05778298250525182 1104 62-63 0.0449686866181338 1104 64-65 0.2982185328331042 1104 66-67 0.1733606327222077 1104 68-69 0.12006300238063261 1104 70-71 0.1686846410789471 1104 72-73 0.24022467955464322 1104 74-75 0.22148518597344236 1104 76-77 0.20612909443486416 1104 78-79 0.22049952642884563 1104 80-81 0.12010567901026548 1104 82-83 0.18036330755152363 1104 84-85 0.1574506681810064 1104 86-87 0.1787391167102541 1104 88-89 0.2565234546909849 1104 90-91 0.2643853172927031 1104 92-93 0.12996003741014306 1104 94-95 0.2398047551823339 1104 96-97 0.3071763858867058 1104 98-99 0.31622246048949876 1104 100-101 0.356186057768376 1104 102-103 0.2869046030757687 1104 104-105 0.2126518446670005 1104 106-107 0.15768039517334742 1104 108-109 0.2535852635130986 1104 110-111 0.20906426646539344 1104 112-113 0.22443733535454413 1104 114-115 0.1877854205229994 1104 116-117 0.22540773819149962 1104 118-119 0.2570238865147303 1104 120-121 0.30300837845391726 1104 122-123 0.23455396976999765 1104 124-125 0.33727933517128506 1105 1 -0.13012948922362 1105 2 -0.20187972102782936 1105 3 -0.13743329445009067 1105 4 -0.1238647945705793 1105 5 -0.28836430671499613 1105 6 -5.959046049852645E-4 1105 7 -0.12314945854821246 1105 8 0.01290750823488196 1105 9 -0.03819906824508479 1105 10-11 0.031332934099452814 1105 12-13 -0.015618967414148699 1105 14-15 -0.12679781179159022 1105 16-17 -0.006191067496352787 1105 18-19 0.10887475131061564 1105 20-21 0.08436052967707752 1105 22-23 0.007542102867930112 1105 24-25 0.13433756289116872 1105 26-27 0.0785690832784951 1105 28-29 0.0586209627799974 1105 30-31 -0.2120916275385838 1105 32-33 -0.03984362189372348 1105 34-35 -0.10981347220217685 1105 36-37 -0.02910541897774266 1105 38-39 0.23679418231777305 1105 40-41 0.2817981660474871 1105 42-43 0.21561627990150356 1105 44-45 0.03355870390016946 1105 46-47 -0.12173140985218822 1105 48-49 0.016774384930236863 1105 50-51 -0.10594564458224909 1105 52-53 -0.014681733872599523 1105 54-55 0.13820884982666826 1105 56-57 -0.008338379303516774 1105 58-59 0.08142168218736145 1105 60-61 0.06061339943792632 1105 62-63 -0.03751287123288449 1105 64-65 -0.008279402728874174 1105 66-67 0.054417999001252326 1105 68-69 -0.15554537938538004 1105 70-71 -0.0938946515496113 1105 72-73 -0.028600337792539676 1105 74-75 -0.1976097584909624 1105 76-77 -0.0832155510674184 1105 78-79 -0.16304894480435905 1105 80-81 -0.11031021051892509 1105 82-83 -0.13454985810055575 1105 84-85 -0.22663273609943957 1105 86-87 -0.15365853489921477 1105 88-89 -0.14017295235024818 1105 90-91 -0.08626157276305335 1105 92-93 -0.01613500055681527 1105 94-95 0.04728791241918273 1105 96-97 0.026297166583841403 1105 98-99 0.048751507796868765 1105 100-101 0.09688772063752538 1105 102-103 0.11536973400395567 1105 104-105 0.028469770583448906 1105 106-107 0.08471862787660811 1105 108-109 0.13971465136662786 1105 110-111 0.08117393020067354 1105 112-113 0.10007647454048296 1105 114-115 0.08733759248896433 1105 116-117 0.11896245933242966 1105 118-119 0.20654037987359075 1105 120-121 0.05320522594140442 1105 122-123 0.04267245227271843 1105 124-125 0.29243280239371927 1106 1 0.058068097651620576 1106 2 -0.04217827543321562 1106 3 0.04020392302599873 1106 4 -0.11915295028749995 1106 5 -0.1677758864114054 1106 6 -0.06771216548035852 1106 7 -0.0019256174417421335 1106 8 -0.2097674315156297 1106 9 0.03485024394752401 1106 10-11 -0.01745588406700449 1106 12-13 -0.05992322832097585 1106 14-15 -0.13826880357878935 1106 16-17 -0.09015113967779342 1106 18-19 -0.025432959444650294 1106 20-21 0.06700268112443553 1106 22-23 -0.1674163220354572 1106 24-25 -0.044070956170472186 1106 26-27 -0.051908902560420245 1106 28-29 -0.040477162310921244 1106 30-31 -0.10211738837268314 1106 32-33 -0.10804840332469468 1106 34-35 0.012802839782068531 1106 36-37 -0.11089041577360348 1106 38-39 -0.13157075660713247 1106 40-41 -0.14486700011529052 1106 42-43 -0.0599895075878365 1106 44-45 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-0.7452124970277083 2216 70-71 -0.6609904808470972 2216 72-73 -0.5518351056785775 2216 74-75 -0.5432111877635251 2216 76-77 -0.5906475606443777 2216 78-79 -0.6518749625508136 2216 80-81 -0.6809194950776387 2216 82-83 -0.6180563539677735 2216 84-85 -0.6697656579283056 2216 86-87 -0.6481273747318816 2216 88-89 -0.6659850331311574 2216 90-91 -0.7568628072764554 2216 92-93 -0.6316700299394817 2216 94-95 -0.5807584454546699 2216 96-97 -0.648723802485808 2216 98-99 -0.6693631715906001 2216 100-101 -0.6556928382433611 2216 102-103 -0.72962794187006 2216 104-105 -0.7392890188974803 2216 106-107 -0.5898777662224539 2216 108-109 -0.6395011703482432 2216 110-111 -0.6121492064085992 2216 112-113 -0.6243743695457233 2216 114-115 -0.7035118430989868 2216 116-117 -0.7825971720274332 2216 118-119 -0.702314929650651 2216 120-121 -0.7577823068755123 2216 122-123 -0.8163206168979471 2216 124-125 -1.135720543179147 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 22.0 5 60.0 6 150.0 7 368.0 8 768.0 9 1454.0 10 3073.0 11 4225.0 12 4924.0 13 7651.0 14 13612.0 15 14204.0 16 5141.0 17 3660.0 18 3485.0 19 3450.0 20 3312.0 21 3152.0 22 3189.0 23 2694.0 24 3120.0 25 3918.0 26 4915.0 27 5864.0 28 7383.0 29 9153.0 30 11338.0 31 14492.0 32 19113.0 33 25538.0 34 36128.0 35 62040.0 36 131005.0 37 355748.0 38 229645.0 39 915.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.69420921562914 19.98071440801944 14.312692943536073 21.012383432815344 2 15.203436628404043 22.24488079720809 40.57470021604067 21.9769823583472 3 17.1307952671797 30.102402950454348 31.141549128688943 21.625252653677006 4 11.40661210184765 18.00368507903683 39.46611440577349 31.12358841334203 5 11.273548921890361 40.417648601731145 34.229805842154896 14.078996634223598 6 27.703807544997122 39.57423586251784 18.31126242271009 14.41069416977495 7 25.68774827011789 35.64708402742183 22.70850685545874 15.956660847001539 8 26.41774250384418 33.832509770630445 21.160322430804715 18.58942529472066 9 28.765797187339825 14.755654151717067 20.76349067785751 35.71505798308559 10-11 23.625154167734493 28.073493961430035 27.897452428241927 20.40389944259354 12-13 25.833256583162477 26.00629484879549 28.709335757303943 19.451112810738085 14-15 22.97174325666325 26.058301431957968 28.088510299205538 22.881445012173245 16-17 20.046160222321884 29.813036583803175 28.499006919528448 21.64179627434649 18-19 20.32506366927217 26.824985584315737 32.851091988083034 19.998858758329064 20-21 21.90808599756535 25.80102151140441 32.88202564390056 19.408866847129676 22-23 21.607659133136853 25.816137958098412 32.63340514479754 19.942797763967196 24-25 20.690599313945686 26.26733093035793 32.964594571731624 20.07747518396477 26-27 20.32391241670938 26.397970992439774 32.883627386596615 20.394489204254228 28-29 20.601448285148095 25.97522988911363 33.06804297303398 20.355278852704284 30-31 20.663309106909463 26.033435768773895 32.823909322859855 20.479345801456788 32-33 20.294930884802664 26.213570364556638 32.706484655304976 20.785014095335725 34-35 20.863099051768323 26.014303562275753 32.74302441055868 20.37957297539723 36-37 21.04806606023687 25.703506784669035 32.10876093007704 21.139666225017056 38-39 20.926768323936443 26.060253555868783 32.114190238980015 20.898787881214762 40-41 20.817409750513434 25.747726400186604 32.434855340037636 21.000008509262333 42-43 20.845379773193233 25.88456240389544 31.748141978472578 21.521915844438748 44-45 20.939013432712546 26.20362560599879 31.408920602582423 21.448440358706236 46-47 21.706280796807643 26.109536023129763 30.482607624894133 21.701575555168457 48-49 21.409193202203998 26.82798885187084 29.562463960789337 22.20035398513583 50-51 21.739857331138225 26.9619721559957 29.236454792411347 22.06171572045473 52-53 21.67463199961558 26.96473763454639 29.313292862634547 22.047337503203483 54-55 21.53560836430529 27.12142258262317 29.093316314399768 22.24965273867177 56-57 21.549982580978426 27.30787019425212 28.770917842283772 22.371229382485673 58-59 21.417137273785723 26.452191288904555 29.4199443981151 22.710727039194623 60-61 21.652862446497906 27.24290839160104 29.097731075738093 22.00649808616296 62-63 21.59022536443645 26.97320274590012 29.68506443775022 21.75150745191321 64-65 21.480794046950443 26.872266321481092 29.399589341294224 22.247350290274237 66-67 21.535462886815186 27.310955285783862 28.979122754240745 22.174459073160207 68-69 21.685954550005004 26.753328661527682 29.525828411252377 22.034888377214937 70-71 21.888968867914137 26.528547673227727 29.27579381963473 22.306689639223404 72-73 21.706698328921707 26.901186934113948 28.670425558327562 22.721689178636787 74-75 21.435471793311123 27.221617119425932 28.747527309712968 22.595383777549973 76-77 21.318442152346954 27.029125299957652 29.26430411326161 22.38812843443379 78-79 21.27975773350686 26.66247872659926 29.130243267594352 22.927520272299528 80-81 21.15694280306955 27.276472269482138 28.68440903245893 22.88217589498938 82-83 21.65145320433759 26.77375822908491 28.604529400458105 22.970259166119394 84-85 21.249018932598666 26.581520694515632 28.733411952203998 23.4360484206817 86-87 21.062042142304737 27.065080552920655 28.691556271915225 23.181321032859383 88-89 21.337510749722437 26.515848519766976 28.911772685543465 23.23486804496713 90-91 21.185844234115358 26.543162291336706 28.83117520208078 23.439818272467157 92-93 21.260596534523366 26.668602221048655 28.60497420790357 23.465827036524413 94-95 21.11277576745357 26.05740578242547 28.915731402888163 23.9140870472328 96-97 21.241517949210813 26.174672681439297 28.842226346866575 23.74158302248332 98-99 20.856639138535346 26.263919660171574 28.817137988721107 24.06230321257197 100-101 20.859423610322015 26.277524626935527 28.921250371049666 23.941801391692792 102-103 21.19531321172538 25.86593358848245 28.780760301653928 24.15799289813824 104-105 20.79878065567288 26.11469144732067 28.859467562988193 24.227060334018255 106-107 20.976693619351476 25.84134122058922 28.63903791228648 24.542927247772823 108-109 21.06225070882791 25.808193970557614 28.3183258045397 24.811229516074775 110-111 20.766362961976682 25.96479882108787 28.56939773968427 24.69944047725118 112-113 20.481628574946864 25.992054950588724 28.93150298189638 24.594813492568036 114-115 20.477929987826755 26.30527013390569 28.292382111737567 24.924417766529984 116-117 20.207055483421446 26.29723769184642 28.444676252438907 25.051030572293225 118-119 20.17778592008393 26.02092505941548 28.42081974335718 25.380469277143412 120-121 19.87414262667702 26.26987356699838 28.446449299410308 25.409534506914298 122-123 19.984212531165735 26.365723673244446 28.254063120843213 25.396000674746606 124-125 19.954049880117058 26.391423311974073 27.86196736219471 25.792559445714158 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 864.0 1 582.0 2 523.5 3 685.0 4 375.0 5 248.0 6 635.5 7 1508.5 8 2598.5 9 2788.5 10 1795.0 11 971.0 12 900.5 13 1041.0 14 1198.0 15 1315.5 16 1473.5 17 1655.0 18 1769.0 19 1888.0 20 2128.5 21 2377.0 22 2735.0 23 3253.0 24 3690.5 25 4467.5 26 5462.0 27 6644.5 28 7967.0 29 9573.5 30 12481.5 31 15408.5 32 19147.0 33 22657.0 34 25496.0 35 28668.5 36 34045.5 37 38634.5 38 38539.5 39 37966.0 40 36178.5 41 34629.5 42 33805.0 43 34496.0 44 35937.5 45 35503.5 46 34560.5 47 32961.0 48 30988.5 49 29626.5 50 28812.5 51 27382.0 52 25948.0 53 25763.0 54 26129.0 55 25099.0 56 23182.0 57 21348.0 58 20363.0 59 19346.0 60 16632.0 61 13721.0 62 11360.0 63 8145.0 64 5433.5 65 4242.0 66 3207.5 67 2315.5 68 1642.0 69 1068.0 70 734.0 71 564.5 72 464.5 73 545.5 74 432.5 75 226.0 76 166.5 77 103.0 78 68.5 79 25.0 80 11.0 81 11.5 82 5.0 83 1.5 84 1.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02392603152229626 2 0.002602831881086622 3 0.0015016337775499745 4 0.08339072911327523 5 0.0034037032291132753 6 0.023725813685289594 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0011512525627883137 26-27 0.0 28-29 6.507079702716555E-4 30-31 0.007157787672988212 32-33 0.0 34-35 0.0 36-37 5.505990517683239E-4 38-39 0.0 40-41 5.005445925166581E-5 42-43 0.0 44-45 0.0044548468733982575 46-47 0.002602831881086622 48-49 0.0 50-51 0.0025527774218349563 52-53 0.0 54-55 0.002452668503331625 56-57 4.0043567401332647E-4 58-59 0.0025027229625832906 60-61 2.50272296258329E-4 62-63 0.0041044656586365965 64-65 0.0029031586365966173 66-67 1.5016337775499745E-4 68-69 0.0012013070220399796 70-71 0.0039042478216299334 72-73 0.0036039210661199385 74-75 0.0 76-77 0.002102287288569964 78-79 0.0012013070220399796 80-81 3.503812147616607E-4 82-83 0.0016017426960533059 84-85 0.0 86-87 0.003453757688364941 88-89 0.00490533700666325 90-91 0.0054058815991799075 92-93 0.005956480650948231 94-95 0.004054411199384931 96-97 0.003804138903126602 98-99 0.00550599051768324 100-101 0.005656153895438236 102-103 0.001902069451563301 104-105 0.0014515793182983087 106-107 1.0010891850333162E-4 108-109 0.008008713480266529 110-111 0.0025027229625832906 112-113 0.005105554843669913 114-115 0.0 116-117 1.0010891850333162E-4 118-119 0.0014015248590466428 120-121 3.503812147616607E-4 122-123 0.001852014992311635 124-125 0.04384770630445925 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 998912.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.529440557280342 #Duplication Level Percentage of deduplicated Percentage of total 1 61.68195830221577 18.83115679444141 2 14.47265009674279 8.83683821669653 3 7.0303176707107005 6.438949962802797 4 4.145011732224163 5.061795571526688 5 2.700943336631376 4.122914452213501 6 1.8207627035158553 3.335212003554021 7 1.3586054472978009 2.9034224970852835 8 1.0158000148771107 2.4809444937820184 9 0.7780692839525932 2.137861795848874 >10 4.4477962882298545 24.67060198042455 >50 0.34854325546406145 7.368718872850331 >100 0.1893678584141942 10.25182875173373 >500 0.008204846551420777 1.585096765463255 >1k 0.0013127754482273241 0.6828340364495628 >5k 6.563877241136621E-4 1.2918238051275441 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7332 0.7339985904664275 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5561 0.5567056957970271 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3557 0.35608742311635055 No Hit CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT 1112 0.11132111737570476 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1111 0.11122100845720143 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1035 0.10361273065094823 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.5016337775499745E-4 0.0 20-21 0.0 0.0 0.0 4.0043567401332647E-4 0.0 22-23 0.0 0.0 0.0 0.0013514703997949769 0.0 24-25 0.0 0.0 0.0 0.003003267555099949 0.0 26-27 0.0 0.0 0.0 0.005155609302921579 0.0 28-29 0.0 0.0 0.0 0.009960837391081497 0.0 30-31 0.0 0.0 0.0 0.027029407995899535 0.0 32-33 0.0 0.0 0.0 0.056461430035879036 0.0 34-35 0.0 0.0 0.0 0.09355178434136341 0.0 36-37 0.0 0.0 0.0 0.14756054587391082 0.0 38-39 0.0 0.0 0.0 0.24141265697078423 0.0 40-41 0.0 0.0 0.0 0.3594410718862122 0.0 42-43 0.0 0.0 0.0 0.46465554523321373 1.0010891850333163E-4 44-45 0.0 0.0 0.0 0.5840354305484368 1.0010891850333163E-4 46-47 0.0 0.0 0.0 0.7241378619938492 1.0010891850333163E-4 48-49 0.0 0.0 0.0 0.881459027421835 1.0010891850333163E-4 50-51 0.0 0.0 0.0 1.0617051351870836 1.0010891850333163E-4 52-53 0.0 0.0 0.0 1.2370959604049205 1.0010891850333163E-4 54-55 0.0 0.0 0.0 1.413638038185546 1.0010891850333163E-4 56-57 0.0 0.0 0.0 1.605797107252691 1.0010891850333163E-4 58-59 0.0 0.0 0.0 1.8258865645822655 1.0010891850333163E-4 60-61 0.0 0.0 0.0 2.0853188268836496 1.0010891850333163E-4 62-63 0.0 0.0 0.0 2.3396455343413636 1.0010891850333163E-4 64-65 0.0 0.0 0.0 2.5890168503331625 1.0010891850333163E-4 66-67 0.0 0.0 0.0 2.8607625096104563 1.0010891850333163E-4 68-69 0.0 0.0 0.0 3.1731523898001024 1.0010891850333163E-4 70-71 0.0 0.0 0.0 3.5294900852127116 1.0010891850333163E-4 72-73 0.0 0.0 0.0 3.9058495643259867 1.0010891850333163E-4 74-75 0.0 0.0 0.0 4.274700874551512 1.0010891850333163E-4 76-77 0.0 0.0 0.0 4.636694723859559 1.0010891850333163E-4 78-79 0.0 0.0 0.0 5.065861657483342 1.0010891850333163E-4 80-81 0.0 0.0 0.0 5.565855650948231 1.0010891850333163E-4 82-83 0.0 0.0 0.0 6.100036840082009 1.0010891850333163E-4 84-85 0.0 0.0 0.0 6.629713127883137 1.0010891850333163E-4 86-87 0.0 0.0 0.0 7.167298020246028 1.0010891850333163E-4 88-89 0.0 0.0 0.0 7.748530401076371 1.0010891850333163E-4 90-91 0.0 0.0 0.0 8.399989188236802 1.0010891850333163E-4 92-93 0.0 0.0 0.0 9.118871332009226 1.0010891850333163E-4 94-95 0.0 0.0 0.0 9.840206144284982 1.0010891850333163E-4 96-97 0.0 0.0 0.0 10.562942481419785 1.0010891850333163E-4 98-99 0.0 0.0 0.0 11.30770278062532 1.0010891850333163E-4 100-101 0.0 0.0 0.0 12.147866879164532 1.0010891850333163E-4 102-103 0.0 0.0 0.0 13.069769909661712 1.0010891850333163E-4 104-105 0.0 0.0 0.0 13.989570652870324 1.0010891850333163E-4 106-107 0.0 0.0 0.0 14.88819835981548 1.0010891850333163E-4 108-109 0.0 0.0 0.0 15.787376665812404 1.0010891850333163E-4 110-111 0.0 0.0 0.0 16.78275964249103 1.0010891850333163E-4 112-113 0.0 0.0 0.0 17.851372293054844 1.0010891850333163E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3680 0.0 74.30696 1 GTATCAA 6880 0.0 66.15605 1 ATCAACG 9135 0.0 49.549484 3 TCAACGC 9275 0.0 48.801567 4 TATCAAC 9280 0.0 48.775272 2 CAACGCA 9545 0.0 47.380756 5 AACGCAG 9660 0.0 46.81201 6 ACGCAGA 11265 0.0 40.243996 7 CGCAGAG 11355 0.0 39.820232 8 GTACATG 7970 0.0 38.12207 1 TCGACCG 40 1.19843084E-4 37.184105 18-19 GCAGAGT 12020 0.0 37.072742 9 ACATGGG 7980 0.0 35.82386 3 TACATGG 8565 0.0 35.045902 2 ATGGGAG 2195 0.0 34.97192 5 CATGGGA 5900 0.0 34.32108 4 GTGGTAT 1100 0.0 34.120277 1 ATCCGCG 45 2.3942016E-4 33.05419 52-53 GTCCGTA 75 0.0038919004 31.763826 2 GAGTACT 7400 0.0 30.79246 12-13 >>END_MODULE