FastQCFastQC Report
Fri 27 May 2016
SRR940103_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940103_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561183
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21350.067.748051
GTATCAA41750.064.157061
ATCAACG52850.049.2180983
TCAACGC52900.049.0590634
TATCAAC55550.047.6830862
CAACGCA55150.047.056055
AACGCAG56900.045.5941936
GTACATG127400.041.255291
TACATGG132200.038.901892
ACGCAGA69450.037.2646187
ACATGGG135900.036.4411663
CGCAGAG70700.036.188498
CATGGGA84500.034.1643684
CATGGGG52350.030.6997934
GCAGAGT83050.030.0189729
ATGGGAG33500.029.849635
CGACCGT603.698172E-529.74982818-19
ATGGGAT34500.029.5020035
GTATAGA4850.028.2276341
ATGGGGG25850.026.479625
Produced by FastQC (version 0.11.5)