FastQCFastQC Report
Fri 27 May 2016
SRR940103_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940103_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561183
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69440.4447909053583084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53190.34070317188952226No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33880.2170149175336908No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37150.079.050831
GTATCAA75650.063.230251
TCAACGC101550.046.9140624
ATCAACG102600.046.4339453
TATCAAC103200.046.2808722
CAACGCA105150.045.332535
AACGCAG107400.044.379996
ACGCAGA123750.038.4597367
CGCAGAG124600.038.1018838
GTACATG113800.036.641571
GTGGTAT13750.035.0913931
TGGTATC13100.035.0046962
TACATGG119850.034.584232
ACATGGG118200.033.8063283
GCAGAGT139400.033.6725359
CATGGGG47300.030.720034
GAGTACT78200.029.09954812-13
CATGGGA74750.028.4413384
AGAGTAC131600.028.09614810-11
AGTACTT81100.027.5088212-13
Produced by FastQC (version 0.11.5)