FastQCFastQC Report
Fri 27 May 2016
SRR940102_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940102_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483745
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22400.076.5379641
GTATCAA47050.058.833651
TCAACGC56900.046.9733854
ATCAACG57500.046.4832273
CAACGCA58950.045.338345
AACGCAG60750.044.0825656
TATCAAC62750.043.92232
GTACATG133100.037.6140751
TACATGG132950.037.1627242
ACGCAGA76650.034.931177
CGCAGAG77100.034.6536258
ACATGGG140350.034.0157243
CATGGGG38400.031.7790154
CATGGGA101650.030.276174
GCAGAGT84250.030.2294739
ATGGGAG42350.027.830115
GTCGGGA5350.026.7039342
ATGGGGA21300.024.8721625
GAGTACA97500.024.7276521
GTATAGT2752.1063897E-923.8118111
Produced by FastQC (version 0.11.5)