FastQCFastQC Report
Fri 27 May 2016
SRR940102_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940102_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483745
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67220.45304280722091733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55010.37075103875665966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30240.20380860592622047No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39100.086.24891
GTATCAA86500.064.196851
ATCAACG115100.047.8715823
TCAACGC115950.047.519044
TATCAAC117900.046.8357242
CAACGCA119450.046.1345255
AACGCAG122900.044.8379446
TGGTATC10250.043.0050282
ACGCAGA143750.038.282777
CGCAGAG144550.038.029748
GTACATG130900.035.6398621
TACATGG130100.035.117972
GCAGAGT155000.034.8516779
ACATGGG132800.033.1031723
CATGGGA93850.029.9574184
GAGTACT83750.029.19621312-13
CATGGGG38400.029.1624034
GTAAGGT6200.028.8221444
AGAGTAC150450.028.59009410-11
AGTACTT85700.028.39304512-13
Produced by FastQC (version 0.11.5)