FastQCFastQC Report
Fri 27 May 2016
SRR940101_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940101_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2035139
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29950.053.848191
ATCAACG37000.042.1403583
TCAACGC37050.041.7622454
GGTATCA24150.041.6455151
CAACGCA39350.039.4724855
AACGCAG40750.038.5478636
TATCAAC41000.038.1742552
GTACATG84500.036.199711
TACATGG85650.035.018852
ACATGGG86350.033.6322673
ACGCAGA48150.032.2488947
CATGGGG62400.029.7555984
CGCAGAG53050.029.0487638
GTATAAC4800.024.7963311
GCAGAGT63900.022.9059099
ATGGGGA34950.022.3060575
AGTACAT62200.021.910072
ATGGGGG35750.021.473975
GAGTACA70300.021.2479521
GTGGTAT12400.020.6369481
Produced by FastQC (version 0.11.5)