FastQCFastQC Report
Fri 27 May 2016
SRR940101_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940101_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2035139
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25580.12569166037307525No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50750.065.1516951
GGTATCA31650.052.8933831
TCAACGC65800.048.8786354
ATCAACG67050.047.878573
CAACGCA69450.046.26545
AACGCAG70300.045.704886
TATCAAC70650.045.6929322
ACGCAGA83350.038.5422977
CGCAGAG87100.036.7462968
GTACATG82350.035.014711
ACATGGG80200.034.1613243
TACATGG84000.033.8221362
GCAGAGT100650.031.090059
CATGGGG61850.028.503834
GTGGTAT12050.025.2145881
GAGTACT55050.024.63922112-13
CAGAGTA95650.024.47423710-11
AGAGTAC95600.024.4248110-11
AGTACTT59400.022.98506212-13
GTACTTT58950.022.75685114-15
Produced by FastQC (version 0.11.5)