FastQCFastQC Report
Fri 27 May 2016
SRR940100_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940100_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697026
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20400.067.978351
GTATCAA40150.062.7046361
ATCAACG51550.046.9905553
TCAACGC52200.046.4054264
CAACGCA53800.045.2465935
TATCAAC56900.043.5124132
AACGCAG55550.043.4895026
GTACATG116700.039.7293471
TACATGG121800.037.429462
TATTCCG2550.037.344335
ACGCAGA67000.035.6968357
ACATGGG123750.035.1574253
CGCAGAG68150.034.663088
CCGTTGC2950.034.2901469
GTACCGT1501.4782381E-731.7351956
CATGGGA78450.031.4088674
CATGGGG49300.030.3020254
GCAGAGT78200.029.447389
TCCGTTG3450.027.5958218
ATGGGAG34800.027.0223265
Produced by FastQC (version 0.11.5)