FastQCFastQC Report
Fri 27 May 2016
SRR940100_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940100_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697026
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45140.26599474610288826No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34670.20429857880786742No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20310.11967995776140143No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32900.085.4525451
GTATCAA70650.062.7248541
ATCAACG95450.046.4831123
TCAACGC96650.045.9675984
CAACGCA97900.045.4053545
TATCAAC98850.044.7651252
AACGCAG101550.043.770776
GTACATG117100.037.5894931
ACGCAGA119900.036.866887
CGCAGAG122050.036.119958
TGGTATC10750.036.010782
TACATGG120800.035.891542
TATTCCG2650.033.6832925
ACATGGG124300.033.202973
GCAGAGT133300.032.669899
GTGGTAT13050.031.949671
CATGGGA78850.029.8339144
CCGTTGC3100.028.7869959
CGTATAG1254.8486763E-528.5903931
GAGTACT66200.028.39852712-13
Produced by FastQC (version 0.11.5)