FastQCFastQC Report
Fri 27 May 2016
SRR940099_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940099_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207424
Sequences flagged as poor quality0
Sequence length125
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40721.9631286639925951No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10480.5052452946621413No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10080.48596112311015116No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT5280.2545510644862697No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG4960.23912372724467756No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4900.23623110151187904No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4610.2222500771366862No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT4230.2039301141622956No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC4060.19573434125269976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3800.18319962974390622No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG3520.1697007096575131No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG3470.16729018821351435No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3230.15571968528232027No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC3150.15186285097192226No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3040.14655970379512498No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT2780.13402499228633138No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG2740.13209657513113235No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG2710.1306502622647331No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA2630.12679342795433507No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA2600.1253471150879358No Hit
ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA2460.11859765504473928No Hit
TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA2450.11811555075593952No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT2420.11666923788954027No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC2390.115222925023141No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT2330.1123302992903425No Hit
GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG2320.11184819500154274No Hit
TATATAATCCCAACAAACGACCTAAAACCTGGTGAACTACGACTGCTAGA2210.10654504782474546No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT2190.10558083924714595No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2180.1050987349583462No Hit
ACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2160.10413452638074669No Hit
ACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTT2150.10365242209194694No Hit
TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA2110.10172400493674792No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4200.086.528871
CGCAGAA658.913048E-1173.325862
GTATCAA8750.061.2794651
GTACAAG1100.059.577261
GTATGGT603.1124546E-759.577261
AGATAGT507.415918E-659.5197756
CGCAAAG401.7677824E-459.5054248
GAACAAA4450.053.5525931
TGGGACA803.8162398E-852.0798076
GGGACCG350.007784491451.004659
TCACTAC909.574251E-846.3378682
ATATAGG2450.046.202772
TCAACGC11450.044.7479864
CACCCGA604.1654857E-1044.6290710-11
ATCAACG11700.044.301043
TCTAGAT951.4646139E-743.8990333
ACAACAC2050.043.5931175
TATAGGC2750.043.3289153
CAACGCA12050.043.0142825
TACAAGG705.3949876E-542.5551832
Produced by FastQC (version 0.11.5)