##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940099_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 207424 Sequences flagged as poor quality 0 Sequence length 125 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.514231718605366 33.0 31.0 34.0 31.0 34.0 2 32.61233994137612 34.0 31.0 34.0 31.0 34.0 3 32.64474699166924 34.0 31.0 34.0 31.0 34.0 4 36.16351531163221 37.0 35.0 37.0 35.0 37.0 5 36.183508176488736 37.0 35.0 37.0 35.0 37.0 6 36.287015967294046 37.0 37.0 37.0 35.0 37.0 7 36.236496258870716 37.0 37.0 37.0 35.0 37.0 8 36.24810050910213 37.0 37.0 37.0 35.0 37.0 9 38.00637341869793 39.0 39.0 39.0 35.0 39.0 10-11 37.990075883215056 39.0 39.0 39.0 35.0 39.0 12-13 38.00513682119716 39.0 39.0 39.0 35.0 39.0 14-15 39.25933112850972 41.0 39.0 41.0 36.0 41.0 16-17 39.27761011261956 41.0 39.0 41.0 36.0 41.0 18-19 39.48887062249305 41.0 39.5 41.0 36.0 41.0 20-21 39.48027470302376 41.0 39.0 41.0 36.0 41.0 22-23 39.366570406510334 41.0 39.0 41.0 35.0 41.0 24-25 39.29721970456649 41.0 39.0 41.0 35.0 41.0 26-27 39.081012804689905 41.0 39.0 41.0 35.0 41.0 28-29 38.89431068728787 41.0 38.5 41.0 35.0 41.0 30-31 38.39670674560321 40.0 37.5 41.0 35.0 41.0 32-33 37.83247117016353 40.0 35.0 41.0 34.0 41.0 34-35 37.51367006710892 40.0 35.0 41.0 33.5 41.0 36-37 37.238754917463744 40.0 35.0 41.0 33.0 41.0 38-39 36.95746875964208 40.0 35.0 41.0 33.0 41.0 40-41 36.71393136763345 40.0 35.0 41.0 33.0 41.0 42-43 36.32028357374267 40.0 35.0 41.0 31.5 41.0 44-45 35.954612291730946 40.0 35.0 41.0 29.5 41.0 46-47 35.03707140928726 40.0 35.0 41.0 19.0 41.0 48-49 34.200473426411605 40.0 35.0 41.0 4.5 41.0 50-51 33.9065730098735 40.0 35.0 41.0 2.0 41.0 52-53 33.832124055075596 40.0 35.0 41.0 2.0 41.0 54-55 33.718349853440294 40.0 35.0 41.0 2.0 41.0 56-57 33.58587964748534 39.0 35.0 41.0 2.0 41.0 58-59 33.39054786331379 39.0 35.0 41.0 2.0 41.0 60-61 33.15579682196853 39.0 35.0 41.0 2.0 41.0 62-63 32.9027547439062 38.5 34.5 41.0 2.0 41.0 64-65 32.58456591329836 38.0 34.0 41.0 2.0 41.0 66-67 32.242059742363466 37.0 34.0 40.0 2.0 41.0 68-69 31.845868848349276 37.0 33.5 40.0 2.0 41.0 70-71 31.505011474082075 36.0 33.0 39.5 2.0 41.0 72-73 31.2460226396174 36.0 33.0 39.0 2.0 41.0 74-75 30.918382154427647 35.5 33.0 39.0 2.0 41.0 76-77 30.58737176025918 35.0 33.0 37.5 2.0 40.0 78-79 30.243486771058315 35.0 33.0 37.0 2.0 39.0 80-81 29.922897061092257 35.0 33.0 37.0 2.0 39.0 82-83 29.61572913452638 35.0 32.5 36.0 2.0 38.5 84-85 29.324077734495525 35.0 32.0 36.0 2.0 37.0 86-87 29.1054795973465 35.0 32.0 36.0 2.0 37.0 88-89 28.88801681579759 35.0 32.0 35.0 2.0 36.5 90-91 28.709336913761184 35.0 31.5 35.0 2.0 36.0 92-93 28.55302906124653 35.0 31.0 35.0 2.0 36.0 94-95 28.40957893011416 35.0 31.0 35.0 2.0 36.0 96-97 28.290173750385684 35.0 31.0 35.0 2.0 36.0 98-99 28.20099168852206 35.0 31.0 35.0 2.0 35.0 100-101 28.122476184048132 35.0 31.0 35.0 2.0 35.0 102-103 27.38662835544585 34.0 28.0 35.0 2.0 35.0 104-105 27.888862908824436 35.0 30.0 35.0 2.0 35.0 106-107 27.883441646096884 35.0 30.5 35.0 2.0 35.0 108-109 27.823547419777846 35.0 30.0 35.0 2.0 35.0 110-111 27.71982750308547 35.0 30.0 35.0 2.0 35.0 112-113 27.586274973002162 35.0 29.5 35.0 2.0 35.0 114-115 27.434983415612464 35.0 29.0 35.0 2.0 35.0 116-117 27.263566414686824 35.0 29.0 35.0 2.0 35.0 118-119 27.11777807775378 35.0 29.0 35.0 2.0 35.0 120-121 26.924637457574825 35.0 27.0 35.0 2.0 35.0 122-123 26.709276650725084 35.0 26.0 35.0 2.0 35.0 124-125 25.187251716291268 33.0 13.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21357146966114726 1101 2 0.267457592206533 1101 3 0.22265302954082955 1101 4 0.14055560161066438 1101 5 0.07063532484985302 1101 6 0.29451189434194447 1101 7 0.05195563837512651 1101 8 0.1800180687840225 1101 9 0.2719890466001402 1101 10-11 0.2956238289992683 1101 12-13 0.04584599367733233 1101 14-15 0.2069787541915531 1101 16-17 0.3860149037950791 1101 18-19 0.4564329685419537 1101 20-21 0.2845611179410099 1101 22-23 0.3122408440131963 1101 24-25 0.12754436910311995 1101 26-27 0.3813079136452018 1101 28-29 0.6449011275133216 1101 30-31 0.6291394163178197 1101 32-33 0.5842324264051513 1101 34-35 0.543123757101533 1101 36-37 0.37193406004937657 1101 38-39 0.497679316317047 1101 40-41 0.5729529389653507 1101 42-43 0.5709203197921013 1101 44-45 0.38461910399600185 1101 46-47 0.7295815174903382 1101 48-49 0.8620463546709729 1101 50-51 0.8363770723104693 1101 52-53 1.180860170108481 1101 54-55 0.8438337429984273 1101 56-57 0.8404317938267241 1101 58-59 0.8244423743701006 1101 60-61 0.8015516099287296 1101 62-63 0.5022021202436235 1101 64-65 0.6502388051857793 1101 66-67 0.4880170779158277 1101 68-69 0.38855483002439684 1101 70-71 0.4949170707037105 1101 72-73 0.7293570741573774 1101 74-75 0.5886171645289515 1101 76-77 0.38642481914341076 1101 78-79 0.367155873830022 1101 80-81 0.5269536872118614 1101 82-83 0.3162106480978082 1101 84-85 0.34470235409041905 1101 86-87 0.425804918218315 1101 88-89 0.46810898881610186 1101 90-91 0.391067045795392 1101 92-93 0.42463397943917514 1101 94-95 0.5666344297457009 1101 96-97 0.48901809043970346 1101 98-99 0.5460221125929614 1101 100-101 0.4256103860186613 1101 102-103 0.396517964890613 1101 104-105 0.5894024872100765 1101 106-107 0.6960438322137215 1101 108-109 0.6639415206115942 1101 110-111 0.8074256766388181 1101 112-113 0.9728874375336396 1101 114-115 0.827449309856231 1101 116-117 0.9470624247907331 1101 118-119 1.0885132713930332 1101 120-121 1.031742285136584 1101 122-123 0.8788244809899055 1101 124-125 0.971789659817631 1102 1 0.4641122570475815 1102 2 0.42152190985302695 1102 3 0.46962462017679485 1102 4 0.11738586647631166 1102 5 0.1661023248017841 1102 6 0.13397014555571474 1102 7 0.3472736213583474 1102 8 0.3788595820273031 1102 9 0.4139397267904954 1102 10-11 0.42083413520510504 1102 12-13 0.3952967940426646 1102 14-15 0.4962639534416624 1102 16-17 0.4735683816588505 1102 18-19 0.6054619547458628 1102 20-21 0.5402754379909993 1102 22-23 0.47796814296046364 1102 24-25 0.2669293135822812 1102 26-27 0.5248388947536924 1102 28-29 0.5708192643205052 1102 30-31 0.7541995038052036 1102 32-33 0.9206742857523551 1102 34-35 0.4872183588416661 1102 36-37 0.7787864371103836 1102 38-39 0.7642394278394278 1102 40-41 0.813254818501953 1102 42-43 0.9289456046067954 1102 44-45 0.6399548728754922 1102 46-47 0.5979478588834084 1102 48-49 1.2448637367793225 1102 50-51 1.0073560176548924 1102 52-53 1.1586698610184456 1102 54-55 1.175510664596274 1102 56-57 1.2576973324509595 1102 58-59 1.0456664766630368 1102 60-61 1.1882386262244609 1102 62-63 1.1004091096201591 1102 64-65 1.16660663674854 1102 66-67 1.0351557117667198 1102 68-69 1.0675013761956151 1102 70-71 1.1301860222972273 1102 72-73 1.142837101076573 1102 74-75 1.200728398485598 1102 76-77 1.197461688825669 1102 78-79 1.035803424664035 1102 80-81 0.9273767031230271 1102 82-83 0.901474792692337 1102 84-85 0.8790303356315441 1102 86-87 0.957579181545718 1102 88-89 1.0484940294352434 1102 90-91 1.1567138275095665 1102 92-93 1.1836651287926294 1102 94-95 1.1531483940777711 1102 96-97 1.2607156821332062 1102 98-99 0.9575132436798235 1102 100-101 0.8330816641996925 1102 102-103 0.9317172149987698 1102 104-105 1.2708546816051154 1102 106-107 0.9968478027948855 1102 108-109 1.235908304248568 1102 110-111 1.182011072975886 1102 112-113 1.2508281191660977 1102 114-115 1.3193615340546607 1102 116-117 1.4143507966601767 1102 118-119 1.2111458243901971 1102 120-121 1.436635810108946 1102 122-123 1.3922360081734872 1102 124-125 1.4885120075559364 1103 1 0.1838798061666651 1103 2 0.2759749123435711 1103 3 0.2118993180828923 1103 4 0.16853428451892682 1103 5 0.23717510406569176 1103 6 0.1400581448177718 1103 7 0.1117672105106422 1103 8 0.20371430022672854 1103 9 0.22397800739192064 1103 10-11 0.12641941331597906 1103 12-13 -0.01208605809274843 1103 14-15 0.23067498563426625 1103 16-17 0.18062377322789303 1103 18-19 0.0438901440273014 1103 20-21 0.16203542323221853 1103 22-23 0.3344620088399992 1103 24-25 0.18707044447425858 1103 26-27 0.29427898330641256 1103 28-29 0.429446236002093 1103 30-31 0.34430881106467837 1103 32-33 0.30699127680484395 1103 34-35 0.36235672246125716 1103 36-37 0.5693788640082644 1103 38-39 0.6135672112542352 1103 40-41 0.4773828970924896 1103 42-43 0.2957014389394246 1103 44-45 0.06226946562524205 1103 46-47 0.7562992563559661 1103 48-49 0.8937602869130785 1103 50-51 0.04683577044522025 1103 52-53 0.5992080954986605 1103 54-55 0.2962271575397324 1103 56-57 0.44632730201095683 1103 58-59 0.4294813922612306 1103 60-61 0.5455799692739944 1103 62-63 0.6402635972135542 1103 64-65 0.6617062585546449 1103 66-67 0.5282721216919981 1103 68-69 0.48621851483596856 1103 70-71 0.6903396059149749 1103 72-73 0.4248509835597396 1103 74-75 0.48207928862488103 1103 76-77 0.49232032732765063 1103 78-79 0.6702582720028403 1103 80-81 0.45465163163515854 1103 82-83 0.59935206416176 1103 84-85 0.8230045999982991 1103 86-87 0.6225835630603385 1103 88-89 0.5950360919756008 1103 90-91 0.6025963567965356 1103 92-93 0.4129048207029733 1103 94-95 0.3570560133010865 1103 96-97 0.56945680380095 1103 98-99 0.6893414883067024 1103 100-101 0.6734215774918262 1103 102-103 0.45592508327660397 1103 104-105 0.7410488518846101 1103 106-107 0.569402225818596 1103 108-109 0.6243526359522882 1103 110-111 0.47917577180440674 1103 112-113 0.6465884272557538 1103 114-115 0.6028109390910998 1103 116-117 0.7726952759517545 1103 118-119 0.7537445237721734 1103 120-121 0.5126377933208275 1103 122-123 0.653282037137604 1103 124-125 0.4122160016524212 1104 1 0.2748278971147542 1104 2 0.3116784730471309 1104 3 0.28834719453076474 1104 4 0.06879168477632902 1104 5 -0.09122472433413975 1104 6 0.2521130568726875 1104 7 0.04079981454324866 1104 8 0.29468384119627444 1104 9 0.36946277787669146 1104 10-11 0.3179466548432188 1104 12-13 0.40018871668203104 1104 14-15 0.4327556377149193 1104 16-17 0.3998590799631998 1104 18-19 0.4113290114661652 1104 20-21 0.3888479500447417 1104 22-23 0.35520417958216655 1104 24-25 0.3764097838135996 1104 26-27 0.547689536716959 1104 28-29 0.6585217400775889 1104 30-31 0.5608575026133735 1104 32-33 0.5420459743595387 1104 34-35 0.8722988074033466 1104 36-37 1.0966043037337059 1104 38-39 1.236009296196329 1104 40-41 1.3516696838792797 1104 42-43 1.1869498301878139 1104 44-45 1.006740722596497 1104 46-47 1.3235397110964229 1104 48-49 2.045513826166612 1104 50-51 1.6788821024915563 1104 52-53 1.6219343932249544 1104 54-55 1.7385855873981626 1104 56-57 1.7914098845935484 1104 58-59 1.5725476728275112 1104 60-61 1.607822569016598 1104 62-63 1.908909028358984 1104 64-65 1.6148486866970728 1104 66-67 1.5480383164582037 1104 68-69 1.5047352458527001 1104 70-71 1.470101510676873 1104 72-73 1.5634449346536883 1104 74-75 1.5178257493713438 1104 76-77 1.5520854495927807 1104 78-79 1.5194753462199166 1104 80-81 1.1264394909230155 1104 82-83 1.2380639903736856 1104 84-85 1.324173626167326 1104 86-87 1.1983289513057258 1104 88-89 1.164453723893235 1104 90-91 1.0980757647759418 1104 92-93 0.8511877464858948 1104 94-95 0.9815573003023736 1104 96-97 1.0850777819219282 1104 98-99 1.2867889357541493 1104 100-101 1.0545562836265283 1104 102-103 1.0297506946022175 1104 104-105 1.0649334507692103 1104 106-107 1.12380484272121 1104 108-109 0.7116220482216988 1104 110-111 0.7149597700884058 1104 112-113 0.9579086760760056 1104 114-115 1.1070044182845784 1104 116-117 1.1217997375562128 1104 118-119 1.0269159094435594 1104 120-121 0.9958156139986443 1104 122-123 1.0583013421569092 1104 124-125 1.2304806949171159 1105 1 0.04805608435568587 1105 2 0.0630079645040098 1105 3 0.23074937833422382 1105 4 0.0854735167447842 1105 5 -0.3521690171255685 1105 6 0.1614043317690559 1105 7 0.04482367461805836 1105 8 0.21993912377639901 1105 9 0.13177194782917212 1105 10-11 0.2567344417201767 1105 12-13 0.15931822835931087 1105 14-15 0.2182373888017608 1105 16-17 0.18381924577050057 1105 18-19 0.2548989235074117 1105 20-21 0.32940502213048006 1105 22-23 0.13451365284068828 1105 24-25 0.2479242919523159 1105 26-27 0.23432890584040678 1105 28-29 0.26691826216838876 1105 30-31 0.25963416826901664 1105 32-33 0.1147787617420093 1105 34-35 0.1569211913887898 1105 36-37 -0.040934873295917384 1105 38-39 0.06139601139195605 1105 40-41 -0.1371484336012614 1105 42-43 0.09110512287814032 1105 44-45 -0.15133227305611996 1105 46-47 -0.12465056289003229 1105 48-49 -0.11018832730623984 1105 50-51 -0.09359287476048905 1105 52-53 0.12935528090061865 1105 54-55 0.17909210847793133 1105 56-57 0.33999445642652404 1105 58-59 -0.021962487924689356 1105 60-61 0.2978286351373143 1105 62-63 0.26626419972689774 1105 64-65 0.4925506379658984 1105 66-67 0.3304971173883331 1105 68-69 0.2588450426376525 1105 70-71 0.03826954222070711 1105 72-73 0.03561660586994719 1105 74-75 -0.06373324365261723 1105 76-77 -0.0941080596580619 1105 78-79 -0.1358830604123753 1105 80-81 0.08028642247695572 1105 82-83 0.087785959840879 1105 84-85 0.042771341261858 1105 86-87 -0.1383154729183147 1105 88-89 0.016440378427660107 1105 90-91 0.12763293796368558 1105 92-93 0.26004933783156403 1105 94-95 0.24230949927113343 1105 96-97 0.415848179252837 1105 98-99 0.46180662860623656 1105 100-101 0.30978325764331416 1105 102-103 0.24432454962193262 1105 104-105 0.06302767784432817 1105 106-107 0.2956782630022765 1105 108-109 0.18768109179666936 1105 110-111 0.14451188817555405 1105 112-113 0.3973738464328953 1105 114-115 0.29167735327598265 1105 116-117 0.17864939886447573 1105 118-119 0.04319838846489077 1105 120-121 0.234330425443396 1105 122-123 0.1816862375163275 1105 124-125 0.15703008324994272 1106 1 0.21494333723019565 1106 2 0.02084153221019136 1106 3 0.16951602569960045 1106 4 0.09368345966809954 1106 5 -0.021599904709319162 1106 6 0.04958780434743204 1106 7 0.1604246341680664 1106 8 -0.06015021363778317 1106 9 0.04983795825187087 1106 10-11 0.11325690015346623 1106 12-13 0.10170386819717692 1106 14-15 0.044732549691829604 1106 16-17 -0.008762853658737413 1106 18-19 0.1001818253189839 1106 20-21 0.27470653590332716 1106 22-23 -0.07524315086516253 1106 24-25 0.056893189297014146 1106 26-27 -0.031098342070919216 1106 28-29 -0.08415502759917359 1106 30-31 -0.22676876001288804 1106 32-33 -0.45027004295647544 1106 34-35 -0.09653236142782617 1106 36-37 -0.10083436870495888 1106 38-39 -0.2600945999075748 1106 40-41 -0.45002584281624536 1106 42-43 -0.677227658989672 1106 44-45 -0.6180789022231323 1106 46-47 -1.168740231183527 1106 48-49 -1.6841398534870677 1106 50-51 -1.669819196209744 1106 52-53 -1.4078996616091004 1106 54-55 -1.564228265415693 1106 56-57 -1.5307041125204535 1106 58-59 -1.2772358769560412 1106 60-61 -1.122264011069987 1106 62-63 -1.1447706253206675 1106 64-65 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-1.4630132728230194 2216 78-79 -1.6282985600905775 2216 80-81 -1.618430814922899 2216 82-83 -1.518885293404864 2216 84-85 -1.4235228507974433 2216 86-87 -1.4263322953067359 2216 88-89 -1.185139772041726 2216 90-91 -1.4685909018907246 2216 92-93 -1.4738606468551758 2216 94-95 -1.2500338727522085 2216 96-97 -1.4161998022824847 2216 98-99 -1.7541324734599435 2216 100-101 -1.9107553694900012 2216 102-103 -1.9370321160830315 2216 104-105 -2.2251555929783713 2216 106-107 -2.114483968738334 2216 108-109 -2.218652825955612 2216 110-111 -2.5041265636320773 2216 112-113 -2.4331095198202384 2216 114-115 -2.551028876334083 2216 116-117 -2.6993771884987616 2216 118-119 -2.4098243151015453 2216 120-121 -2.5842463294779314 2216 122-123 -2.827412943557377 2216 124-125 -3.235643966329498 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 11.0 5 40.0 6 82.0 7 219.0 8 423.0 9 743.0 10 1484.0 11 2001.0 12 2351.0 13 3583.0 14 6529.0 15 6594.0 16 2017.0 17 1346.0 18 1230.0 19 1445.0 20 1434.0 21 1644.0 22 1665.0 23 1036.0 24 1084.0 25 1273.0 26 1502.0 27 1595.0 28 1902.0 29 2256.0 30 2730.0 31 3296.0 32 4203.0 33 5270.0 34 7427.0 35 15843.0 36 19553.0 37 59930.0 38 43583.0 39 98.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.526671617464096 22.158997752852336 16.836247552730814 17.478083076952753 2 14.884364498912827 24.55223484830223 41.90937185118046 18.654028801604483 3 14.688412993800078 34.650133544175645 33.74617928667162 16.915274175352664 4 9.1554697865155 21.723796888192016 41.45024725606079 27.670486069231696 5 8.083038443366664 40.82209215994446 35.99521747934163 15.099651917347243 6 21.52817960884157 44.65243220306292 18.453689773560157 15.365698414535355 7 20.064216291268128 39.37538568343104 25.164397562480715 15.396000462820117 8 24.229115242209197 33.91748302992904 22.466059858068498 19.387341869793275 9 27.10631363776612 14.521945387226165 23.4394284171552 34.93231255785251 10-11 21.05927954335082 29.87262804689911 28.793196544276455 20.274895865473617 12-13 25.379657127429805 29.440421551990127 29.547448704103672 15.632472616476395 14-15 22.446775686516506 27.79596382289417 32.09850354828757 17.65875694230176 16-17 15.482538182659672 31.14803494291885 33.10561940759025 20.263807466831224 18-19 15.347307929651341 25.270942610305465 42.396733261339094 16.985016198704102 20-21 17.27379666769516 24.656018589941375 43.21486423943227 14.855320502931194 22-23 17.235469376735576 24.622994446158593 43.09698974082073 15.044546436285097 24-25 16.24421769199432 24.983909575429742 43.931559650277336 14.840313082298604 26-27 15.622830530700401 25.5811767201481 43.51111732489972 15.284875424251773 28-29 16.260895556926876 23.412430576982413 44.54740049367479 15.779273372415922 30-31 15.991630044091732 24.689682438280013 44.165499485315216 15.153188032313045 32-33 15.786504936747917 25.5888903887689 43.21076635297747 15.413838321505708 34-35 16.279456572045664 23.749662526997838 44.37528926257328 15.595591638383215 36-37 15.997946230779059 24.585208522660164 42.29607542057675 17.120769825984038 38-39 15.789638614625115 24.636734418389388 42.890167000925636 16.683459966059857 40-41 15.474824514038875 24.306010876272754 43.22113159518667 16.998033014501697 42-43 16.224737735266892 24.12690913298365 41.73215249922863 17.916200632520827 44-45 15.978680628878058 24.529570864490374 41.16441757425862 18.32733093237295 46-47 16.68004541542823 24.58772963838809 39.51648478800107 19.215740158182612 48-49 16.75866823511262 25.728459580376427 37.3073993366245 20.205472847886455 50-51 17.510082176062635 25.82170036230749 36.044653252691994 20.623564208937882 52-53 17.119041190990437 26.1734418389386 36.327522369639 20.379994600431967 54-55 17.22668312285007 26.31454612512324 35.9238162275003 20.534954524526384 56-57 17.639997396630566 26.573652455001483 34.692549301308674 21.093800847059278 58-59 16.808768618489403 25.93390752646182 36.54973086203696 20.707592993011815 60-61 18.037208809009563 27.767278617710584 34.12213630052453 20.073376272755322 62-63 18.588051519775522 27.661626059768917 35.00702695796601 18.74329546248954 64-65 18.769781866130554 26.467745162557826 35.88228629558115 18.880186675730467 66-67 18.626822354211665 27.119330453563716 34.9602746066029 19.29357258562172 68-69 18.721209126300185 27.108197037447724 35.24738155214598 18.92321228410611 70-71 18.819907818104678 26.322222007945385 35.62092606163459 19.236944112315346 72-73 18.322618281397272 26.80016488403879 34.73846354110169 20.13875329346225 74-75 17.59536022832459 27.451982412835545 34.932553609996916 20.02010374884295 76-77 17.363079741587118 26.71367274129785 36.04763282229293 19.8756146948221 78-79 17.041063529752076 26.605358627921273 35.954392604466726 20.399185237859925 80-81 17.22413992594878 27.788250154273374 34.63196158593027 20.35564833384758 82-83 17.868773819366936 26.008759982740376 35.00883471016606 21.11363148772662 84-85 17.12844222462203 26.095099892008637 35.02535868559087 21.751099197778466 86-87 17.27060593203996 27.160392640876307 34.161540093338985 21.407461333744745 88-89 17.8558772693195 25.86548127291618 35.0343637227085 21.24427773505582 90-91 17.512842703102738 25.892480479814477 35.13970884248132 21.45496797460146 92-93 17.98171285861335 26.797677103949358 34.052267571782934 21.16834246565436 94-95 17.625122338091863 25.619886508820393 34.75848170593538 21.996509447152356 96-97 18.034857652532363 26.061278113926186 34.28271822192223 21.621146011619217 98-99 17.744816563853714 26.424736820536555 34.18259483982634 21.64785177578339 100-101 17.6764121651592 25.971014213256705 34.605037317030835 21.747536304553254 102-103 18.591129656279605 25.71695798169428 33.860038617018965 21.831873745007147 104-105 17.77917053357278 26.463618942014488 33.93749472694621 21.819715797466525 106-107 18.155324359765505 25.43799174637458 34.34366322122801 22.063020672631904 108-109 18.147410646699484 25.815184636969825 33.10351145332607 22.933893263004624 110-111 18.389981679683736 26.433082634268633 32.67476617491081 22.502169511136824 112-113 18.214931417689172 26.521225562278524 33.30978955234675 21.954053467685558 114-115 18.077464517124344 26.66976820425794 32.68932235421166 22.56344492440605 116-117 17.743125192841717 26.824523680962663 32.90723349274915 22.525117633446467 118-119 17.730166497525556 26.375647537249215 33.1100349290451 22.784151036180127 120-121 17.680933739586546 26.772215365627893 32.55891314409133 22.98793775069423 122-123 17.616274304668327 27.217371600480188 32.448570285267984 22.7177838095835 124-125 17.56949033188476 27.379407806840273 32.01667864429342 23.03442321698154 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1184.0 1 838.5 2 818.5 3 979.5 4 504.0 5 324.5 6 801.5 7 1842.0 8 3001.5 9 3134.5 10 1887.5 11 798.5 12 595.5 13 553.0 14 561.5 15 595.0 16 643.0 17 683.0 18 713.0 19 742.5 20 758.0 21 768.5 22 811.0 23 880.0 24 941.0 25 1005.5 26 1148.0 27 1379.5 28 1549.0 29 2036.5 30 3080.0 31 3866.5 32 5076.5 33 6825.5 34 8035.5 35 8955.5 36 10707.0 37 12172.5 38 12174.0 39 12078.5 40 11405.0 41 9992.0 42 8078.0 43 7441.0 44 7489.0 45 6773.0 46 5857.5 47 4966.5 48 4351.0 49 3739.0 50 3277.5 51 2374.0 52 1653.5 53 1487.0 54 1429.5 55 1415.5 56 1504.0 57 1367.0 58 1159.5 59 1170.0 60 1148.5 61 1158.0 62 1046.0 63 590.5 64 239.0 65 288.5 66 316.5 67 202.5 68 107.5 69 59.0 70 62.0 71 64.5 72 62.0 73 92.5 74 80.0 75 40.5 76 26.0 77 26.0 78 19.0 79 2.5 80 0.5 81 1.5 82 1.5 83 0.5 84 0.5 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024105214439987657 2 0.002410521443998766 3 9.642085775995063E-4 4 0.07183353903116323 5 9.642085775995063E-4 6 0.019284171551990126 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001205260721999383 26-27 0.0 28-29 0.0 30-31 0.007472616476396174 32-33 0.0 34-35 0.0 36-37 2.4105214439987659E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0033747300215982725 46-47 0.0026515735883986423 48-49 0.0 50-51 0.0016873650107991362 52-53 0.0 54-55 0.002169469299598889 56-57 2.4105214439987659E-4 58-59 0.001205260721999383 60-61 0.0 62-63 0.004097886454797902 64-65 0.0026515735883986423 66-67 0.0 68-69 7.231564331996298E-4 70-71 0.0038568343103980254 72-73 0.0026515735883986423 74-75 0.0 76-77 0.0019284171551990127 78-79 7.231564331996298E-4 80-81 0.0 82-83 0.0016873650107991362 84-85 0.0 86-87 0.0038568343103980254 88-89 0.0050620950323974085 90-91 0.0050620950323974085 92-93 0.0050620950323974085 94-95 0.0033747300215982725 96-97 0.004338938599197778 98-99 0.004097886454797902 100-101 0.005785251465597038 102-103 0.001205260721999383 104-105 7.231564331996298E-4 106-107 0.0 108-109 0.008195772909595804 110-111 0.0019284171551990127 112-113 0.004338938599197778 114-115 0.0 116-117 0.0 118-119 0.0016873650107991362 120-121 0.0 122-123 0.002410521443998766 124-125 0.04483569885837704 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 207424.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.44523295279235 #Duplication Level Percentage of deduplicated Percentage of total 1 69.30860033726813 19.02190681888306 2 11.251053962900507 6.175755939524838 3 5.113476672287802 4.210216754088244 4 3.0424395727937044 3.34001851280469 5 1.9533445756042722 2.6804998457266276 6 1.4421725688589095 2.3748457266275844 7 1.0223440134907251 1.9640928725701945 8 0.8308740865654862 1.8242826288182659 9 0.6183249016301293 1.527306386917618 >10 4.640949971894322 25.460409595803764 >50 0.5006323777403036 9.519149182351127 >100 0.25470770095559303 11.924367479173096 >500 0.010539629005059021 2.037372724467757 >1k 0.008783024170882519 5.245776766430114 >5k 0.0017566048341765036 2.6939987658130207 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5588 2.6939987658130207 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4320 2.0826905276149335 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3057 1.4737928108608456 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1278 0.6161292810860846 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1189 0.5732219993829065 No Hit CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT 1037 0.499942147485344 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 932 0.44932119716137 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 834 0.4020749768589941 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 660 0.3181888306078371 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 654 0.31529620487503857 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 582 0.28058469608145636 No Hit ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT 564 0.2719068188830608 TruSeq Adapter, Index 3 (95% over 21bp) GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 445 0.21453640851589015 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 425 0.20489432273989508 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 425 0.20489432273989508 No Hit ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT 393 0.189466985498303 RNA PCR Primer, Index 3 (95% over 23bp) TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 392 0.18898488120950324 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 363 0.17500385683431038 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 356 0.1716291268127121 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 340 0.16391545819191608 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 319 0.15379126812712127 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 309 0.14897022523912373 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 307 0.14800601666152421 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 293 0.1412565566183277 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 281 0.13547130515273062 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 272 0.13113236655353286 No Hit TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG 264 0.12727553224313484 TruSeq Adapter, Index 3 (96% over 28bp) GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 263 0.12679342795433507 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 261 0.12582921937673558 No Hit CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC 255 0.12293659364393705 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 245 0.11811555075593952 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 239 0.115222925023141 No Hit ACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTT 239 0.115222925023141 No Hit TATATAATCCCAACAAACGACCTAAAACCTGGTGAACTACGACTGCTAGA 239 0.115222925023141 No Hit GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG 235 0.113294507867942 No Hit ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA 233 0.1123302992903425 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 226 0.10895556926874422 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 226 0.10895556926874422 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 222 0.10702715211354522 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 222 0.10702715211354522 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 219 0.10558083924714595 No Hit GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT 218 0.1050987349583462 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 215 0.10365242209194694 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 215 0.10365242209194694 No Hit TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA 213 0.10268821351434743 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.4105214439987659E-4 0.0 14-15 0.0 0.0 0.0 4.8210428879975317E-4 0.0 16-17 0.0 0.0 0.0 7.231564331996298E-4 0.0 18-19 0.0 0.0 0.0 0.001205260721999383 0.0 20-21 0.0 0.0 0.0 0.001687365010799136 0.0 22-23 0.0 0.0 0.0 0.005785251465597038 0.0 24-25 0.0 0.0 0.0 0.016632597963591483 0.0 26-27 0.0 0.0 0.0 0.027720996605985808 0.0 28-29 0.0 0.0 0.0 0.05303147176797285 0.0 30-31 0.0 0.0 0.0 0.1205260721999383 0.0 32-33 0.0 0.0 0.0 0.20634063560629434 0.0 34-35 0.0 0.0 0.0 0.2984225547670472 0.0 36-37 0.0 0.0 0.0 0.4230465134217834 0.0 38-39 0.0 0.0 0.0 0.6354134526380746 0.0 40-41 0.0 0.0 0.0 0.8971960814563407 0.0 42-43 0.0 0.0 0.0 1.1206514193150263 0.0 44-45 0.0 0.0 0.0 1.360980407281703 0.0 46-47 0.0 0.0 0.0 1.607335698858377 0.0 48-49 0.0 0.0 0.0 1.8852688213514348 0.0 50-51 0.0 0.0 0.0 2.2155102591792657 0.0 52-53 0.0 0.0 0.0 2.507665458191916 0.0 54-55 0.0 0.0 0.0 2.7759564949089786 0.0 56-57 0.0 0.0 0.0 3.0849853440296204 0.0 58-59 0.0 0.0 0.0 3.43788568343104 0.0 60-61 0.0 0.0 0.0 3.8570753625424254 0.0 62-63 0.0 0.0 0.0 4.255534557235421 0.0 64-65 0.0 0.0 0.0 4.625549598889232 0.0 66-67 0.0 0.0 0.0 5.022321428571429 0.0 68-69 0.0 0.0 0.0 5.457179497068806 0.0 70-71 0.0 0.0 0.0 5.963389000308547 0.0 72-73 0.0 0.0 0.0 6.484543736501079 0.0 74-75 0.0 0.0 0.0 6.959657513113237 0.0 76-77 0.0 0.0 0.0 7.471893319962975 0.0 78-79 0.0 0.0 0.0 8.039812172169084 0.0 80-81 0.0 0.0 0.0 8.695232952792349 0.0 82-83 0.0 0.0 0.0 9.373312634989201 0.0 84-85 0.0 0.0 0.0 10.016680808392472 0.0 86-87 0.0 0.0 0.0 10.66607528540574 0.0 88-89 0.0 0.0 0.0 11.323424483184201 0.0 90-91 0.0 0.0 0.0 12.100094492440604 0.0 92-93 0.0 0.0 0.0 12.966676951558162 0.0 94-95 0.0 0.0 0.0 13.78938792039494 0.0 96-97 0.0 0.0 0.0 14.60824205492132 0.0 98-99 0.0 0.0 0.0 15.443969839555692 0.0 100-101 0.0 0.0 0.0 16.379252159827214 0.0 102-103 0.0 0.0 0.0 17.397215365627893 0.0 104-105 0.0 0.0 0.0 18.407947007096574 0.0 106-107 0.0 0.0 0.0 19.36082613390929 0.0 108-109 0.0 0.0 0.0 20.330096806541192 0.0 110-111 0.0 0.0 0.0 21.336730561555076 0.0 112-113 0.0 0.0 0.0 22.38289686825054 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1300 0.0 94.00271 1 GTATCAC 20 8.408931E-4 89.417206 1 TCACGCA 20 8.4250886E-4 89.37402 4 TATCACG 20 8.4250886E-4 89.37402 2 ATCACGC 20 8.4250886E-4 89.37402 3 TAGTCAA 55 1.9826984E-9 75.722824 9 GGTATAT 50 8.7793524E-8 71.53377 1 AGTGTGC 25 0.002054943 71.395805 8 GATAGTC 60 3.949026E-9 69.41259 7 GTGGTAT 260 0.0 64.196976 1 AGATAGT 65 7.4342097E-9 64.07316 6 GTATCAA 2755 0.0 62.74892 1 CACGCAG 30 0.0042326497 59.496506 5 TAGATAG 80 3.8280632E-8 52.05944 5 GTTCGAC 45 9.708492E-8 46.27506 16-17 ATCAACG 3840 0.0 45.152504 3 TCAACGC 3900 0.0 44.457855 4 TATACTC 95 1.4803118E-7 43.83953 5 CAACGCA 3985 0.0 43.595932 5 TATCAAC 3980 0.0 43.414524 2 >>END_MODULE