FastQCFastQC Report
Fri 27 May 2016
SRR940098_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940098_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1892273
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG23070.1219168692889451No Hit
GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG22670.1198030093966357No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG19960.10548160862623944No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG19780.10453037167470021No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG19770.10447752517739248No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18700.060.1574861
GTATCAA35050.055.8698351
ATCAACG42550.044.761893
TCAACGC42900.044.3966984
CAACGCA44050.043.371625
TATCAAC46850.041.7969252
AACGCAG47250.040.551676
GTACATG106200.039.0640951
TACATGG112450.036.3626252
ACATGGG114000.033.936463
ACGCAGA59450.032.224777
CGCAGAG59600.031.8467168
CATGGGG57500.031.5711174
TAAGGTG12000.026.2870085
GCAGAGT68900.026.2527399
CATGGGA59600.026.1645364
ATGGGGA29450.025.6663975
ACTTGAC12050.025.1827628
GAGTACA82400.025.0652331
GTAAGGT10950.024.4599974
Produced by FastQC (version 0.11.5)