FastQCFastQC Report
Fri 27 May 2016
SRR940098_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940098_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1892273
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46880.24774437937866262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36880.1948978820709274No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG24700.13053084835010592No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21780.11509967113624725No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG21650.1144126666712467No Hit
GTCTATGTCTTGTTATTTTGACCGAGATGATGTGGCTCTGAAGAACTTTG21010.11103049084355164No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG19780.10453037167470021No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG19370.10236366528508307No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG19120.10104250285238969No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC19070.10077827036585102No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32950.074.6750951
GTATCAA72000.063.300931
ATCAACG89850.050.381753
TCAACGC90850.049.827194
TATCAAC92300.049.239322
CAACGCA93100.048.581865
AACGCAG96450.046.954936
CGCAGAG112500.040.1385548
ACGCAGA113750.039.749787
GTACATG96150.036.310271
GCAGAGT125100.035.9055989
TACATGG101100.033.9359932
ACATGGG101350.032.5584533
TGGTATC15900.032.2174032
GTGGTAT17250.031.4292071
CATGGGG53250.029.4180474
GAGTACT74450.028.36848412-13
AGAGTAC119800.027.85983710-11
CAGAGTA121500.027.61692610-11
AGTACTT76650.026.70046212-13
Produced by FastQC (version 0.11.5)