FastQCFastQC Report
Fri 27 May 2016
SRR940097_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940097_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167533
Sequences flagged as poor quality0
Sequence length125
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48382.887789271367432No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36932.2043418311616216No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28311.6898163346922697No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13910.8302841828177134No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12680.7568658115117618No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11950.7132923065903434No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT11420.6816567482227383No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9430.562874180012296No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8000.47751786215253117No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT6390.38141739239433425TruSeq Adapter, Index 10 (95% over 21bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG5830.34799114204365705TruSeq Adapter, Index 10 (96% over 28bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5030.30023935582840394No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG4740.28292933332537473No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT4380.26144102952851084RNA PCR Primer, Index 10 (95% over 23bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4320.2578596455623668No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT3790.2262240871947616No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3770.22503029253938028No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT3550.2118985513301857No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC3520.2101078593471137No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA3200.19100714486101245No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT2950.17608471166874587No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC2910.1736971223579832No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA2870.17130953304722055No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG2830.16892194373645789No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA2720.1623560731318606No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT2590.15459640787188195No Hit
TATATAATCCCAACAAACGACCTAAAACCTGGTGAACTACGACTGCTAGA2510.14982122925035662No Hit
ACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTT2430.14504605062883133No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG2410.14385225597345No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG2360.1408677693349967No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG2350.14027087200730604No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT2350.14027087200730604No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC2330.13907707735192468No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2320.138480180024234No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT2320.138480180024234No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC2310.13788328269654337No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA2310.13788328269654337No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG2310.13788328269654337No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC2290.13668948804116204No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA2210.13191430941963672No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA2210.13191430941963672No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT2070.12355774683196744No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC2060.12296084950427677No Hit
GAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGA2040.12176705484889544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2040.12176705484889544No Hit
TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA2040.12176705484889544No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT2020.12057326019351412No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT2010.11997636286582346No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA1960.11699187622737012RNA PCR Primer, Index 10 (96% over 28bp)
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA1870.11161980027815416No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT1870.11161980027815416No Hit
GCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTG1830.10923221096739151No Hit
ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC1810.10803841631201017No Hit
TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA1810.10803841631201017No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC1740.10386013501817552No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT1740.10386013501817552TruSeq Adapter, Index 10 (96% over 26bp)
CCCTAGGACTTAAAACTGATGCCATCCCAGGCCGACTAAATCAAGCAACA1720.10266634036279419No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG1710.10206944303510354No Hit
GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT1700.10147254570741288No Hit
TTCATATATAATCCCAACAAACGACCTAAAACCTGGTGAACTACGACTGC1700.10147254570741288No Hit
GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG1690.10087564837972221No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA1680.10027875105203155No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9700.095.8152541
GTGGTAT1550.076.874111
TGGGTAT250.002052912371.4075246
GTATCAA20500.061.9024051
TATCTCG150.004232168759.50626824-25
TACGTCA150.004232168759.50626890-91
GGGTATG401.7692818E-459.4885067
GGGATGC300.004233482359.4885067
GCAGATC551.3073521E-554.0804638
CATGGGT350.00774476251.0663644
GGTATGC350.00779064750.9901478
AATAGTC505.316718E-447.605025
GAGTGGG255.3283124E-447.59790818-19
TGCTTGG255.332236E-447.5908114-15
GGAGTGG255.332236E-447.5908116-17
ATCAACG27700.046.027333
TCAACGC27950.045.615634
CAACGCA28250.044.8666765
TATCAAC28800.044.269342
AACGCAG28800.044.0098466
Produced by FastQC (version 0.11.5)