##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940097_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 167533 Sequences flagged as poor quality 0 Sequence length 125 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.53921913891592 34.0 31.0 34.0 31.0 34.0 2 32.65661093635284 34.0 31.0 34.0 31.0 34.0 3 32.70285854130231 34.0 31.0 34.0 31.0 34.0 4 36.20645484770165 37.0 35.0 37.0 35.0 37.0 5 36.20072463335582 37.0 37.0 37.0 35.0 37.0 6 36.28911915861352 37.0 37.0 37.0 35.0 37.0 7 36.241630007222454 37.0 37.0 37.0 35.0 37.0 8 36.256647943987154 37.0 37.0 37.0 35.0 37.0 9 38.038470032769666 39.0 39.0 39.0 35.0 39.0 10-11 38.02447279043532 39.0 39.0 39.0 35.0 39.0 12-13 38.03673306154609 39.0 39.0 39.0 35.0 39.0 14-15 39.34237433819008 41.0 39.0 41.0 36.0 41.0 16-17 39.3514561310309 41.0 39.0 41.0 36.0 41.0 18-19 39.49879128291143 41.0 39.5 41.0 36.0 41.0 20-21 39.49647532127999 41.0 39.0 41.0 36.0 41.0 22-23 39.36798421803466 41.0 39.0 41.0 35.0 41.0 24-25 39.30963750425289 41.0 39.0 41.0 35.0 41.0 26-27 39.0788471525013 41.0 39.0 41.0 35.0 41.0 28-29 38.85294538986349 41.0 38.5 41.0 35.0 41.0 30-31 38.42924379077555 40.0 37.5 41.0 35.0 41.0 32-33 37.898145440002864 40.0 35.5 41.0 34.5 41.0 34-35 37.57393767198104 40.0 35.0 41.0 33.5 41.0 36-37 37.34688688198743 40.0 35.0 41.0 33.0 41.0 38-39 37.08124369527198 40.0 35.0 41.0 33.0 41.0 40-41 36.91858022001635 40.0 35.0 41.0 33.0 41.0 42-43 36.62706451863215 40.0 35.0 41.0 32.5 41.0 44-45 36.37116568079125 40.0 35.0 41.0 30.5 41.0 46-47 35.619430201810985 40.0 35.0 41.0 25.0 41.0 48-49 34.960897256062985 40.0 35.0 41.0 19.0 41.0 50-51 34.746778246673784 40.0 35.0 41.0 15.0 41.0 52-53 34.7247139369557 40.0 35.0 41.0 10.5 41.0 54-55 34.62383530408934 40.0 35.0 41.0 7.5 41.0 56-57 34.50702548154692 40.0 35.0 41.0 4.5 41.0 58-59 34.30921967612351 39.5 35.0 41.0 2.0 41.0 60-61 34.0377507714898 39.0 35.0 41.0 2.0 41.0 62-63 33.757880536968834 39.0 35.0 41.0 2.0 41.0 64-65 33.45228701211104 39.0 35.0 41.0 2.0 41.0 66-67 33.084601242740234 38.0 35.0 41.0 2.0 41.0 68-69 32.65639306882823 37.0 34.5 40.0 2.0 41.0 70-71 32.27481749864206 37.0 34.0 39.5 2.0 41.0 72-73 31.95935129198427 36.5 34.0 39.0 2.0 41.0 74-75 31.600356347704633 36.0 34.0 39.0 2.0 41.0 76-77 31.236463860851295 35.5 34.0 38.5 2.0 40.5 78-79 30.87600651811882 35.0 33.5 37.0 2.0 39.0 80-81 30.537288176060834 35.0 33.0 37.0 2.0 39.0 82-83 30.206848799937923 35.0 33.0 36.5 2.0 39.0 84-85 29.88584338607916 35.0 33.0 36.0 2.0 37.0 86-87 29.644144735664018 35.0 33.0 36.0 2.0 37.0 88-89 29.408185253054622 35.0 33.0 35.5 2.0 36.5 90-91 29.222206968179403 35.0 33.0 35.0 2.0 36.0 92-93 29.041278434696448 35.0 32.0 35.0 2.0 36.0 94-95 28.89263906215492 35.0 32.0 35.0 2.0 36.0 96-97 28.753773883354327 35.0 32.0 35.0 2.0 36.0 98-99 28.6641676565214 35.0 32.0 35.0 2.0 35.5 100-101 28.596575003133708 35.0 32.0 35.0 2.0 35.0 102-103 27.851450162057624 34.0 29.5 35.0 2.0 35.0 104-105 28.347582267374186 35.0 31.0 35.0 2.0 35.0 106-107 28.31234741812061 35.0 31.0 35.0 2.0 35.0 108-109 28.240397414240775 35.0 31.0 35.0 2.0 35.0 110-111 28.11852888684617 35.0 31.0 35.0 2.0 35.0 112-113 27.98439411936753 35.0 31.0 35.0 2.0 35.0 114-115 27.831221311622187 35.0 30.5 35.0 2.0 35.0 116-117 27.658216590164326 35.0 30.0 35.0 2.0 35.0 118-119 27.49079882769365 35.0 29.5 35.0 2.0 35.0 120-121 27.300316952481005 35.0 29.0 35.0 2.0 35.0 122-123 27.06825222493479 35.0 29.0 35.0 2.0 35.0 124-125 25.6065163281264 33.0 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3467215097412861 1101 2 0.3578369873872731 1101 3 0.23680589950200925 1101 4 0.2669893163144508 1101 5 0.23900725452291027 1101 6 0.33593030166780835 1101 7 0.36287580095586236 1101 8 0.3419834110687958 1101 9 0.3743862192165679 1101 10-11 0.21087130193976122 1101 12-13 0.09500659146534929 1101 14-15 0.20819165905260917 1101 16-17 0.29649636375321364 1101 18-19 0.37785569987488543 1101 20-21 0.47400305334686266 1101 22-23 0.41498378366130595 1101 24-25 0.4091448472814392 1101 26-27 0.4522164131215618 1101 28-29 0.47001703459956445 1101 30-31 0.22062142616189107 1101 32-33 0.07564187229881725 1101 34-35 0.059663316644922304 1101 36-37 0.14090930256273992 1101 38-39 0.06940074133655827 1101 40-41 0.3124523055535704 1101 42-43 0.20580460150533497 1101 44-45 0.24601404962923823 1101 46-47 0.1256468574684817 1101 48-49 0.02181536782054394 1101 50-51 0.3527533103428482 1101 52-53 0.47984368034375535 1101 54-55 0.4874224053234215 1101 56-57 0.5996145758682516 1101 58-59 0.7900390305688489 1101 60-61 0.8366605759897254 1101 62-63 0.818573884766252 1101 64-65 0.7380335110754075 1101 66-67 0.9608113040932693 1101 68-69 0.8195905546996443 1101 70-71 0.7471917694674914 1101 72-73 0.8202096625531894 1101 74-75 0.5112784223322109 1101 76-77 0.6612543605308794 1101 78-79 0.835634845792196 1101 80-81 0.8506074605620668 1101 82-83 0.7844495484369993 1101 84-85 0.9288685549806388 1101 86-87 0.8806188227168796 1101 88-89 0.9899784792540771 1101 90-91 0.9614886809784338 1101 92-93 0.9931004621668578 1101 94-95 0.8726841872887476 1101 96-97 0.7978128265550986 1101 98-99 0.9309945862088327 1101 100-101 0.9676842662445324 1101 102-103 0.9083043408169083 1101 104-105 0.7695105378926002 1101 106-107 0.6903177333884294 1101 108-109 0.9432706914650417 1101 110-111 0.7586894022339976 1101 112-113 0.817483692508489 1101 114-115 1.0232812178580488 1101 116-117 0.9100726389724372 1101 118-119 0.4535689964809606 1101 120-121 0.18380686061980356 1101 122-123 -0.014456066007298318 1101 124-125 -0.06061715878200147 1102 1 0.0532871663069443 1102 2 0.19354017309046156 1102 3 0.1412348039309137 1102 4 -0.026794677469546002 1102 5 0.09669956221521403 1102 6 0.19902276476027225 1102 7 0.2011430892231516 1102 8 0.3522786713640542 1102 9 0.3189083637387071 1102 10-11 0.3851331512016074 1102 12-13 0.2417431882019443 1102 14-15 0.4872126380735864 1102 16-17 0.40954997680682226 1102 18-19 0.33869486071404253 1102 20-21 0.32270157704538605 1102 22-23 0.48596280464033015 1102 24-25 0.4331930213296076 1102 26-27 0.6714471823523311 1102 28-29 0.38138067096320327 1102 30-31 0.5759050314454939 1102 32-33 0.24786409452104863 1102 34-35 0.320385927367532 1102 36-37 0.5024477641012055 1102 38-39 0.4246454965813129 1102 40-41 0.14065743375869744 1102 42-43 -0.633725313024577 1102 44-45 -0.8200504414352494 1102 46-47 -0.6326087757871548 1102 48-49 -0.5100805140753479 1102 50-51 -1.1390687564792188 1102 52-53 -1.349293848793785 1102 54-55 -1.1687547508537364 1102 56-57 -1.1944025181488414 1102 58-59 -1.4429640774342616 1102 60-61 -1.7644078000786507 1102 62-63 -1.705940489748123 1102 64-65 -1.5964264733845752 1102 66-67 -1.5480270797451148 1102 68-69 -1.5076821725730838 1102 70-71 -1.6028470805713653 1102 72-73 -1.573788006444481 1102 74-75 -1.4495413134875257 1102 76-77 -1.5693788951023784 1102 78-79 -1.7239999638426156 1102 80-81 -1.7674578075031988 1102 82-83 -1.5760982371107843 1102 84-85 -1.5475494714373923 1102 86-87 -1.6275396854416257 1102 88-89 -1.624085234809641 1102 90-91 -1.4119767424869885 1102 92-93 -1.6200308509644543 1102 94-95 -1.2860959214913592 1102 96-97 -1.5529058991636298 1102 98-99 -1.359254054039809 1102 100-101 -1.2079567593964917 1102 102-103 -1.2234749909624192 1102 104-105 -1.3408818474997872 1102 106-107 -1.2674328493621552 1102 108-109 -1.5776889044945541 1102 110-111 -1.622934529389937 1102 112-113 -1.5747462997215038 1102 114-115 -1.2605571659803338 1102 116-117 -1.2968426679428688 1102 118-119 -1.2612328683209029 1102 120-121 -1.3387261619132218 1102 122-123 -1.3947202462714827 1102 124-125 -1.0182123413771826 1103 1 0.019099132118910234 1103 2 -0.01586153631124887 1103 3 -0.059619896923784665 1103 4 -0.013974164649035004 1103 5 0.17362263913829423 1103 6 0.18192874766624811 1103 7 0.2524251405052027 1103 8 0.15569747478285478 1103 9 -0.018698473868127508 1103 10-11 0.12444939051785298 1103 12-13 0.08362353008229206 1103 14-15 -0.014924114063163074 1103 16-17 0.06553288278973213 1103 18-19 0.06519058720977 1103 20-21 0.05774431208812558 1103 22-23 0.11416793284545435 1103 24-25 0.010116098252687777 1103 26-27 -0.07000580910065679 1103 28-29 -0.054516764934227524 1103 30-31 -0.4775137719732996 1103 32-33 -0.555554708897759 1103 34-35 -0.38687902989742895 1103 36-37 -0.4975522358987945 1103 38-39 -0.33603826410244864 1103 40-41 -0.4661801730789037 1103 42-43 -0.5888535181527814 1103 44-45 -0.9696230910079038 1103 46-47 -1.1561130492914273 1103 48-49 -0.8263198303146595 1103 50-51 -0.6219747393851947 1103 52-53 -0.5565588060587388 1103 54-55 -0.6901222722212594 1103 56-57 -0.6089324326787633 1103 58-59 -0.2634768979470863 1103 60-61 -0.4759462616171106 1103 62-63 -0.9516669854746169 1103 64-65 -0.8101016870597917 1103 66-67 -1.0202493019673327 1103 68-69 -0.8987078135987261 1103 70-71 -0.7631034908277741 1103 72-73 -0.887890570547043 1103 74-75 -0.8191994331456449 1103 76-77 -0.9689515446750256 1103 78-79 -0.9248546646973175 1103 80-81 -0.602927892973284 1103 82-83 -0.7684059294184777 1103 84-85 -0.7462674201553412 1103 86-87 -0.6296764375783823 1103 88-89 -0.7266493373737433 1103 90-91 -0.7752246057348522 1103 92-93 -0.7525094834430881 1103 94-95 -0.9655831009785416 1103 96-97 -0.8798289760867029 1103 98-99 -0.7588267036124563 1103 100-101 -0.5626576140973469 1103 102-103 -0.6850134525008826 1103 104-105 -1.0609673175852592 1103 106-107 -0.9533302852595895 1103 108-109 -1.009312836118486 1103 110-111 -1.0737892302446355 1103 112-113 -1.0042334792086791 1103 114-115 -0.8567110121341805 1103 116-117 -0.8288939499941534 1103 118-119 -0.7697798768679149 1103 120-121 -1.0566748798619408 1103 122-123 -0.7259168274680619 1103 124-125 -0.7254772986421436 1104 1 0.25442095315501234 1104 2 0.16683513209970613 1104 3 0.2618522816912474 1104 4 0.1361915426595317 1104 5 -0.08038050057913182 1104 6 0.11496555213162907 1104 7 -0.26486074820925865 1104 8 -0.006346830118594937 1104 9 -0.14268242642350515 1104 10-11 0.18577853756128349 1104 12-13 0.16699174916479365 1104 14-15 0.2157055737093856 1104 16-17 0.07998430438400561 1104 18-19 0.132493918798815 1104 20-21 0.16653552087932866 1104 22-23 0.2095106853310611 1104 24-25 0.12741942984172994 1104 26-27 0.45731845393788717 1104 28-29 0.2558704668815679 1104 30-31 0.33852495120827797 1104 32-33 0.519519423319224 1104 34-35 0.6399045040289622 1104 36-37 0.8245827719504959 1104 38-39 0.6937977728764437 1104 40-41 0.775717611676022 1104 42-43 0.6991719484441177 1104 44-45 1.2713851071431534 1104 46-47 1.4101088240733048 1104 48-49 1.784616852050597 1104 50-51 1.5914824383576942 1104 52-53 1.704055183126684 1104 54-55 1.6994817745256512 1104 56-57 1.6036962085213062 1104 58-59 1.5598442253740359 1104 60-61 1.1963544535407422 1104 62-63 1.1455683189035426 1104 64-65 1.2661411177544437 1104 66-67 1.3865534191210926 1104 68-69 1.4096647662024253 1104 70-71 1.402664867797725 1104 72-73 1.5235955623676602 1104 74-75 1.660026103222748 1104 76-77 1.728833210252585 1104 78-79 1.7378611908756803 1104 80-81 1.7170267555527943 1104 82-83 1.7438651328525836 1104 84-85 1.5464937868915811 1104 86-87 1.405479676149163 1104 88-89 1.3722604829646485 1104 90-91 1.319280888770642 1104 92-93 1.1317832079924628 1104 94-95 1.341237062984483 1104 96-97 1.2766161660727242 1104 98-99 1.501124456338701 1104 100-101 1.4666638580812688 1104 102-103 1.4852987749542024 1104 104-105 1.4631561779668125 1104 106-107 1.72505799312869 1104 108-109 1.7102929549158752 1104 110-111 1.6107302185605263 1104 112-113 1.6340885162561705 1104 114-115 1.7413702716613884 1104 116-117 1.9802025091023054 1104 118-119 1.939839868466116 1104 120-121 1.8071834839964254 1104 122-123 1.979699778148543 1104 124-125 1.8185572382495394 1105 1 -0.009997857847650948 1105 2 0.060801414264744835 1105 3 0.021390879739165314 1105 4 -0.21917669159068964 1105 5 -0.1370797019988288 1105 6 0.07308445186543366 1105 7 -0.20372498955796914 1105 8 0.10660791699764616 1105 9 -0.2572343341431207 1105 10-11 0.15800568929154224 1105 12-13 0.16872200648511182 1105 14-15 -0.052085020789292 1105 16-17 -0.022278339804103098 1105 18-19 -0.0027767111923111543 1105 20-21 0.0982125395128719 1105 22-23 -0.037783014757664546 1105 24-25 0.1443227128940876 1105 26-27 -0.09343576079147908 1105 28-29 -0.1403584594715781 1105 30-31 -0.16870663707921807 1105 32-33 0.014772307081429403 1105 34-35 0.050374779095513134 1105 36-37 0.04743104169318002 1105 38-39 0.11110034607963826 1105 40-41 0.20593056642314167 1105 42-43 0.02645677541838154 1105 44-45 0.025954761091689704 1105 46-47 -0.05706065241293601 1105 48-49 0.10640034805769716 1105 50-51 0.1692552866274326 1105 52-53 0.2679859728338698 1105 54-55 0.544734658287851 1105 56-57 0.5648319671725943 1105 58-59 0.3915212440075777 1105 60-61 0.6420953585984179 1105 62-63 0.8187715132247533 1105 64-65 0.7878358826169105 1105 66-67 0.5978666400616461 1105 68-69 0.7031873926443097 1105 70-71 0.8347411765821136 1105 72-73 1.1068697416045765 1105 74-75 0.9090056950594843 1105 76-77 1.0352662182383874 1105 78-79 1.2000617232625537 1105 80-81 1.1415165514711596 1105 82-83 1.2128092322314643 1105 84-85 1.0189871320557344 1105 86-87 1.2029508385271548 1105 88-89 1.2959073330090156 1105 90-91 1.034808839081201 1105 92-93 1.0913218060403764 1105 94-95 0.9197197604112759 1105 96-97 0.8413899016539119 1105 98-99 0.5778325308728647 1105 100-101 0.6459451358097503 1105 102-103 0.6193715344927995 1105 104-105 0.6868030280111768 1105 106-107 0.858598365799498 1105 108-109 0.910958438500618 1105 110-111 0.6956459239731245 1105 112-113 0.5315153130618526 1105 114-115 0.6483800320872994 1105 116-117 0.7916931923321222 1105 118-119 0.6533713680224622 1105 120-121 0.44724559579766776 1105 122-123 0.37131468498084175 1105 124-125 0.5509441060401343 1106 1 -0.16778245102823774 1106 2 -0.1387114848873381 1106 3 -0.07458602807248838 1106 4 0.005088146944402183 1106 5 0.058716798091374756 1106 6 0.039023474055582597 1106 7 7.671627061469621E-4 1106 8 -0.3338936164679893 1106 9 0.054997309371223935 1106 10-11 0.03417382928792989 1106 12-13 -0.16119861411745262 1106 14-15 -0.22049562876330953 1106 16-17 -0.3168570877745154 1106 18-19 -0.29847398703572026 1106 20-21 0.02646438869201262 1106 22-23 -0.31566502810783703 1106 24-25 -0.2840464013040034 1106 26-27 -0.1247545412352764 1106 28-29 -0.37899277302019385 1106 30-31 -0.14160037693127947 1106 32-33 -0.25444111525304436 1106 34-35 -0.6041805158711142 1106 36-37 -0.6790077098853899 1106 38-39 -0.6320892624356063 1106 40-41 -0.645224042994144 1106 42-43 -0.3810211246357369 1106 44-45 -0.5068433626680076 1106 46-47 -0.05857607727312342 1106 48-49 0.37785082613815746 1106 50-51 0.18209129600863605 1106 52-53 0.5201869470355618 1106 54-55 0.6354797421774379 1106 56-57 0.6319797210964637 1106 58-59 0.6799390682904658 1106 60-61 0.741328625782252 1106 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52-53 0.4598374960518541 2216 54-55 0.46376748881136365 2216 56-57 0.39621321532403186 2216 58-59 0.26800749068016216 2216 60-61 0.23410955558156132 2216 62-63 0.40373158420966604 2216 64-65 0.4777057436047869 2216 66-67 0.14471668442721608 2216 68-69 0.3460593240255534 2216 70-71 0.5172907181378648 2216 72-73 0.8487656303948725 2216 74-75 0.8192097766921336 2216 76-77 0.3540032782797944 2216 78-79 0.18922173509336915 2216 80-81 0.18845532698136225 2216 82-83 0.3431848607437402 2216 84-85 0.2992148753269568 2216 86-87 0.5384728734280735 2216 88-89 0.42362102718233885 2216 90-91 0.5066958547570373 2216 92-93 0.34028660935300437 2216 94-95 0.3684479473382254 2216 96-97 0.08716038375979807 2216 98-99 0.19874350395775053 2216 100-101 0.15952100093841182 2216 102-103 -0.07490530667844908 2216 104-105 0.06553713034776365 2216 106-107 0.139683843468827 2216 108-109 0.11165349913356337 2216 110-111 0.02297511651971007 2216 112-113 -0.09890468102273786 2216 114-115 -0.16985421813453172 2216 116-117 -0.09938932763238739 2216 118-119 -0.12648666214612803 2216 120-121 -0.031251890153235706 2216 122-123 0.3831011386927585 2216 124-125 -0.24640874814501856 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 11.0 5 33.0 6 94.0 7 182.0 8 339.0 9 610.0 10 1092.0 11 1386.0 12 1520.0 13 2324.0 14 4273.0 15 4286.0 16 1381.0 17 1109.0 18 1213.0 19 1381.0 20 1422.0 21 1694.0 22 1103.0 23 888.0 24 949.0 25 1081.0 26 1237.0 27 1433.0 28 1625.0 29 1809.0 30 2261.0 31 2629.0 32 3369.0 33 4121.0 34 5878.0 35 12965.0 36 12856.0 37 49878.0 38 39037.0 39 63.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.867815714533045 23.236876197754043 16.885869338093503 19.009438749619406 2 14.018468444269349 25.20518835544891 41.804702469423205 18.97164073085854 3 13.42454142267906 35.08347808439136 33.95113681810314 17.540843674826448 4 8.755496081055247 22.438945708277576 42.563563372204165 26.241994838463008 5 8.073229112571555 40.64311253574008 37.164908762065075 14.118749589623288 6 21.270777607948034 46.246895299961785 18.266383263278563 14.215943828811618 7 19.485116365134033 38.87711674714832 26.462846125837896 15.174920761879749 8 23.009795085147402 34.85761014247939 23.577444443781225 18.555150328591978 9 25.557949777058848 15.370703085362287 23.60609551551038 35.465251622068486 10-11 19.614046187915214 30.375508108850198 30.273140217151244 19.73730548608334 12-13 23.65384730172563 29.64281663910991 30.616057731909535 16.08727832725493 14-15 21.225370524016164 28.958175404248713 31.68718998645043 18.129264085284692 16-17 15.288331254140974 31.88535989924373 33.10153820441346 19.724770642201836 18-19 14.84752257764142 26.722496463383333 41.261721571272524 17.168259387702722 20-21 16.104886798421802 26.60072940853444 42.08752902413256 15.2068547689112 22-23 16.365432481958777 25.8966890105233 42.576387935511214 15.16149057200671 24-25 15.91262486532303 26.165981716762023 42.86562745291156 15.055765965003387 26-27 14.944816842054998 27.145696668716013 42.65249234479177 15.256994144437217 28-29 15.551609389222987 24.248727858773673 43.96341008461045 16.236252667392893 30-31 15.96999823905303 25.898754503099564 43.19334302351611 14.937904234331304 32-33 15.892689798427773 26.241397217264655 43.10374672452591 14.762166259781656 34-35 15.05792888565238 25.21831519760286 44.2491926963643 15.474563220380464 36-37 15.854535687105486 25.296882694402576 41.678181845313595 17.17039977317834 38-39 15.128109471296614 25.815289570680317 42.93704206646471 16.119558891558356 40-41 14.600152209272826 25.290913703311297 43.78165430588095 16.327279781534926 42-43 15.254009657798761 25.229656246828984 42.174079136647705 17.342254958724553 44-45 15.028189233473707 25.67503439713243 41.516219628302046 17.780556741091814 46-47 15.7467319286098 25.197875007461352 40.36441234405778 18.690980719871067 48-49 16.052956730912715 26.002936734852238 38.779225585407055 19.164880948827992 50-51 16.246795378779513 26.405183502803954 37.37923912816395 19.96878199025258 52-53 15.605582183808565 26.88186804987674 38.30409531256528 19.20845445374941 54-55 16.222620820562103 27.110424009813077 37.16883093627168 19.49812423335313 56-57 16.94651203046564 27.140921490094488 35.881587508132725 20.030978971307146 58-59 15.437281195252178 27.134027141488517 37.89959380288248 19.52909786037683 60-61 16.987101048748602 29.152465484412026 35.15038828171166 18.710045185127704 62-63 16.943932095338717 29.239116809626992 36.2465602970232 17.57039079801109 64-65 17.428818719035398 27.339580970572435 36.91547782486719 18.31612248552498 66-67 17.313357109814515 28.374196051512396 35.82021398833062 18.49223285034247 68-69 17.28445908841296 27.710825394551485 36.805207363369384 18.199508153666166 70-71 17.643775760849774 26.613103001614668 37.18963865418307 18.553482583352483 72-73 16.94224249080751 26.882968817152953 36.31154195119622 19.863246740843323 74-75 16.37289369855491 27.574865847325604 36.15944321417273 19.89279723994676 76-77 16.392190102130353 26.78519199431747 37.63930257683653 19.183315326715654 78-79 15.785044051285723 26.69102022300217 37.525666738294774 19.99826898741733 80-81 16.797636279527854 27.978451942160476 35.82797367674929 19.395938101562383 82-83 17.17164482009407 26.39973258840102 36.413640379151445 20.01498221235346 84-85 16.343347280834227 26.02651417929602 36.66262766141596 20.967510878453798 86-87 16.70591465725909 27.111556817062578 36.33617769341201 19.846350832266324 88-89 16.988509177734667 25.838233099537383 37.110282047455605 20.062975675272348 90-91 16.65602148932995 25.932547380987913 36.79391135651395 20.617519773168183 92-93 17.59772451193706 26.82891159203579 35.46615569662945 20.1072081993977 94-95 16.772570309771886 25.661611745007505 36.24560890366599 21.32020904155462 96-97 17.402736945783403 26.02157919085496 35.647450342491304 20.928233520870332 98-99 16.70725810784191 26.428311336353815 35.784638527247004 21.07979202855727 100-101 16.739243982151653 25.998298735990687 35.9193541166112 21.34310316524646 102-103 17.54904330853188 25.678010869662536 35.10286186694363 21.67008395486195 104-105 16.835642890612643 26.709485251763134 34.97046771146402 21.484404146160205 106-107 17.272119522720896 25.49467866032364 35.207391976506116 22.025809840449345 108-109 17.594123651645486 25.73648362275324 33.98151851431778 22.687874211283496 110-111 17.360134903224843 26.643088448165226 33.59806598916596 22.39871065944397 112-113 17.17857878352848 26.623708032508485 34.827832180772475 21.369881003190557 114-115 17.15930592778736 26.790244310076222 33.94674482042344 22.103704941712977 116-117 17.133639342696664 26.74577545916327 33.89988838019972 22.220696817940347 118-119 17.432490509275837 25.749110617673043 33.93471098058878 22.883687892462333 120-121 17.107972757605964 26.416885031605712 33.83751260945605 22.637629601332275 122-123 16.949456059452924 26.68069421438271 33.998895703690444 22.37095402247392 124-125 17.04610938101821 26.68330740682131 33.574484729234655 22.696098482925827 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1540.0 1 1055.0 2 901.5 3 1067.0 4 551.0 5 322.0 6 790.0 7 1823.0 8 2924.0 9 3023.0 10 1783.0 11 665.5 12 450.0 13 402.0 14 388.5 15 407.5 16 410.0 17 416.0 18 436.5 19 432.0 20 427.5 21 464.5 22 486.5 23 501.5 24 512.0 25 565.5 26 705.5 27 845.0 28 970.5 29 1410.0 30 2162.5 31 2765.5 32 4089.5 33 5792.5 34 6640.0 35 7602.5 36 10117.5 37 11825.5 38 11263.0 39 10812.0 40 10053.5 41 8438.0 42 6638.0 43 6395.5 44 6819.5 45 6274.0 46 5099.0 47 4026.0 48 3538.5 49 2940.5 50 2416.0 51 1571.0 52 924.5 53 701.5 54 475.0 55 379.0 56 323.0 57 308.5 58 311.0 59 250.0 60 153.0 61 82.5 62 64.0 63 57.0 64 53.5 65 44.5 66 42.0 67 36.0 68 21.5 69 13.5 70 8.5 71 12.0 72 14.0 73 20.5 74 20.5 75 11.5 76 6.0 77 4.0 78 4.0 79 1.0 80 0.5 81 1.5 82 1.5 83 0.5 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019100714486101247 2 0.002387589310762656 3 0.002387589310762656 4 0.08416252320438361 5 0.002387589310762656 6 0.026860379746079875 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0014922433192266597 26-27 0.0 28-29 2.98448663845332E-4 30-31 0.005670524613061308 32-33 0.0 34-35 0.0 36-37 2.98448663845332E-4 38-39 2.98448663845332E-4 40-41 2.98448663845332E-4 42-43 0.0 44-45 0.0026860379746079877 46-47 0.0017906919830719917 48-49 0.0 50-51 8.953459915359959E-4 52-53 0.0 54-55 8.953459915359959E-4 56-57 0.0 58-59 0.0020891406469173237 60-61 0.0 62-63 0.002387589310762656 64-65 0.0017906919830719917 66-67 2.98448663845332E-4 68-69 5.96897327690664E-4 70-71 0.0038798326299893156 72-73 0.0029844866384533195 74-75 0.0 76-77 0.001193794655381328 78-79 5.96897327690664E-4 80-81 2.98448663845332E-4 82-83 5.96897327690664E-4 84-85 0.0 86-87 0.005073627285370644 88-89 0.004775178621525312 90-91 0.004775178621525312 92-93 0.005073627285370644 94-95 0.0038798326299893156 96-97 0.0062674219407519716 98-99 0.005968973276906639 100-101 0.0062674219407519716 102-103 0.0014922433192266597 104-105 0.0026860379746079877 106-107 0.0 108-109 0.008356562587669295 110-111 0.0032829353022986517 112-113 0.004476729957679979 114-115 0.0 116-117 0.0 118-119 5.96897327690664E-4 120-121 0.0 122-123 0.0032829353022986517 124-125 0.043275056257573136 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 167533.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.65861651137388 #Duplication Level Percentage of deduplicated Percentage of total 1 69.32389482808436 14.321357583282099 2 9.399017624963884 3.883414013955459 3 4.533371857844553 2.809595721439955 4 2.7997688529326785 2.313574042129013 5 1.8289511701820285 1.8891800421409513 6 1.3724357122219013 1.7011573839183922 7 0.9968217278243282 1.4415070463729533 8 0.9014735625541751 1.4898557299158972 9 0.7801213522103438 1.4504605062883134 >10 6.680150245593759 28.851032333928238 >50 0.9101415775787344 12.988485850548848 >100 0.4391794279110084 15.026293327284773 >500 0.014446691707598961 2.0700399324312224 >1k 0.020225368390638543 9.764046486363881 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4838 2.887789271367432 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3693 2.2043418311616216 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2831 1.6898163346922697 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1391 0.8302841828177134 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1268 0.7568658115117618 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1195 0.7132923065903434 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 1142 0.6816567482227383 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 943 0.562874180012296 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 800 0.47751786215253117 No Hit ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT 639 0.38141739239433425 TruSeq Adapter, Index 10 (95% over 21bp) TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG 583 0.34799114204365705 TruSeq Adapter, Index 10 (96% over 28bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 503 0.30023935582840394 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 474 0.28292933332537473 No Hit ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT 438 0.26144102952851084 RNA PCR Primer, Index 10 (95% over 23bp) GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 432 0.2578596455623668 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 379 0.2262240871947616 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 377 0.22503029253938028 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 355 0.2118985513301857 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 352 0.2101078593471137 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 320 0.19100714486101245 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 295 0.17608471166874587 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 291 0.1736971223579832 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 287 0.17130953304722055 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 283 0.16892194373645789 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 272 0.1623560731318606 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 259 0.15459640787188195 No Hit TATATAATCCCAACAAACGACCTAAAACCTGGTGAACTACGACTGCTAGA 251 0.14982122925035662 No Hit ACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTT 243 0.14504605062883133 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 241 0.14385225597345 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 236 0.1408677693349967 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 235 0.14027087200730604 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 235 0.14027087200730604 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 233 0.13907707735192468 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 232 0.138480180024234 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 232 0.138480180024234 No Hit CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC 231 0.13788328269654337 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 231 0.13788328269654337 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 231 0.13788328269654337 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 229 0.13668948804116204 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 221 0.13191430941963672 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 221 0.13191430941963672 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 207 0.12355774683196744 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 206 0.12296084950427677 No Hit GAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGA 204 0.12176705484889544 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 204 0.12176705484889544 No Hit TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA 204 0.12176705484889544 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 202 0.12057326019351412 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 201 0.11997636286582346 No Hit CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA 196 0.11699187622737012 RNA PCR Primer, Index 10 (96% over 28bp) CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 187 0.11161980027815416 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 187 0.11161980027815416 No Hit GCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTG 183 0.10923221096739151 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 181 0.10803841631201017 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 181 0.10803841631201017 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 174 0.10386013501817552 No Hit CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT 174 0.10386013501817552 TruSeq Adapter, Index 10 (96% over 26bp) CCCTAGGACTTAAAACTGATGCCATCCCAGGCCGACTAAATCAAGCAACA 172 0.10266634036279419 No Hit CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG 171 0.10206944303510354 No Hit GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT 170 0.10147254570741288 No Hit TTCATATATAATCCCAACAAACGACCTAAAACCTGGTGAACTACGACTGC 170 0.10147254570741288 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 169 0.10087564837972221 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 168 0.10027875105203155 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.98448663845332E-4 0.0 18-19 0.0 0.0 0.0 0.001193794655381328 0.0 20-21 0.0 0.0 0.0 0.00298448663845332 0.0 22-23 0.0 0.0 0.0 0.008356562587669295 0.0 24-25 0.0 0.0 0.0 0.03044176371222386 0.0 26-27 0.0 0.0 0.0 0.05193006750908776 0.0 28-29 0.0 0.0 0.0 0.08565476652361027 0.0 30-31 0.0 0.0 0.0 0.18563506891179649 0.0 32-33 0.0 0.0 0.0 0.29397193388765197 0.0 34-35 0.0 0.0 0.0 0.4205141673580728 0.0 36-37 0.0 0.0 0.0 0.5592927960461521 0.0 38-39 0.0 0.0 0.0 0.7774587693170898 0.0 40-41 0.0 0.0 0.0 1.0388997988456006 0.0 42-43 0.0 0.0 0.0 1.2645269887126716 0.0 44-45 0.0 0.0 0.0 1.4773208860343932 0.0 46-47 0.0 0.0 0.0 1.751296759444408 0.0 48-49 0.0 0.0 0.0 2.057803537213564 0.0 50-51 0.0 0.0 0.0 2.3458064978243094 0.0 52-53 0.0 0.0 0.0 2.611425808646655 0.0 54-55 0.0 0.0 0.0 2.883909438737443 0.0 56-57 0.0 0.0 0.0 3.1426644302913456 0.0 58-59 0.0 0.0 0.0 3.4590200139673977 0.0 60-61 0.0 0.0 0.0 3.791193376827252 0.0 62-63 0.0 0.0 0.0 4.114413279771746 0.0 64-65 0.0 0.0 0.0 4.444497501984683 0.0 66-67 0.0 0.0 0.0 4.764136020963035 0.0 68-69 0.0 0.0 0.0 5.133615466803555 0.0 70-71 0.0 0.0 0.0 5.565172234723905 0.0 72-73 0.0 0.0 0.0 5.993147618678112 0.0 74-75 0.0 0.0 0.0 6.441715960437645 0.0 76-77 0.0 0.0 0.0 6.871482036374923 0.0 78-79 0.0 0.0 0.0 7.3943640954319445 0.0 80-81 0.0 0.0 0.0 7.926796511732016 0.0 82-83 0.0 0.0 0.0 8.491759832391232 0.0 84-85 0.0 0.0 0.0 9.089552506073431 0.0 86-87 0.0 0.0 0.0 9.67898861716796 0.0 88-89 0.0 0.0 0.0 10.274095252875552 0.0 90-91 0.0 0.0 0.0 10.99037204610435 0.0 92-93 0.0 0.0 0.0 11.735896808389988 0.0 94-95 0.0 0.0 0.0 12.492165722574061 0.0 96-97 0.0 0.0 0.0 13.277981054478818 0.0 98-99 0.0 0.0 0.0 14.067377770349722 0.0 100-101 0.0 0.0 0.0 14.878262790017489 0.0 102-103 0.0 0.0 0.0 15.795693982678038 0.0 104-105 0.0 0.0 0.0 16.71879569995165 0.0 106-107 0.0 0.0 0.0 17.633540854637594 0.0 108-109 0.0 0.0 0.0 18.5059062990575 0.0 110-111 0.0 0.0 0.0 19.446019590170295 0.0 112-113 0.0 0.0 0.0 20.40284600645843 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 970 0.0 95.815254 1 GTGGTAT 155 0.0 76.87411 1 TGGGTAT 25 0.0020529123 71.407524 6 GTATCAA 2050 0.0 61.902405 1 TATCTCG 15 0.0042321687 59.506268 24-25 TACGTCA 15 0.0042321687 59.506268 90-91 GGGTATG 40 1.7692818E-4 59.488506 7 GGGATGC 30 0.0042334823 59.488506 7 GCAGATC 55 1.3073521E-5 54.080463 8 CATGGGT 35 0.007744762 51.066364 4 GGTATGC 35 0.007790647 50.990147 8 AATAGTC 50 5.316718E-4 47.60502 5 GAGTGGG 25 5.3283124E-4 47.597908 18-19 TGCTTGG 25 5.332236E-4 47.59081 14-15 GGAGTGG 25 5.332236E-4 47.59081 16-17 ATCAACG 2770 0.0 46.02733 3 TCAACGC 2795 0.0 45.61563 4 CAACGCA 2825 0.0 44.866676 5 TATCAAC 2880 0.0 44.26934 2 AACGCAG 2880 0.0 44.009846 6 >>END_MODULE