FastQCFastQC Report
Fri 27 May 2016
SRR940096_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940096_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344090
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15610.45366037955186145No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC9110.2647563137551222No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG8680.2522595832485687No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT8430.2449940422563864No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA8050.23395041994826934No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA7160.20808509401610045No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG7090.2060507425382894No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT7060.2051788776192275No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC6790.19733209334767068No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG5760.1673980644598797No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG5230.15199511755645326No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG5130.14908890115958034No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG5060.14705454968176931No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT5040.14647330640239473No Hit
ACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTT5020.14589206312302014No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG5010.14560144148333284No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC4940.1435670900055218No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC4920.14298584672614723No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT4810.13978900868958702No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT4760.13833590049115058No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA4720.1371734139324014No Hit
ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC4680.13601092737365222No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA4650.13513906245459037No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA4480.13019849457990643No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT4470.12990787294021913No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC4430.12874538638146998No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA4330.12583916998459704No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC4200.12206108866866228No Hit
TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA4200.12206108866866228No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC4110.11944549391147664No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA4070.1182830073527275No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA4050.1177017640733529No Hit
TATATAATCCCAACAAACGACCTAAAACCTGGTGAACTACGACTGCTAGA4040.11741114243366561No Hit
CTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCT4010.11653927751460373No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT3950.11479554767648No Hit
CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC3900.11334243947804354No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG3890.11305181783835626No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT3780.10985497980179604No Hit
GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT3780.10985497980179604No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT3670.10665814176523584No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3660.10636752012554854No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG3590.10433316864773752No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA3550.10317068208898834No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG3550.10317068208898834No Hit
GCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTG3550.10317068208898834No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT3520.10229881716992646No Hit
CCCTAGGACTTAAAACTGATGCCATCCCAGGCCGACTAAATCAAGCAACA3500.1017175738905519No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5000.077.4341051
AGTTGAC250.002055060871.4048848
GCATCGA250.002055060871.4048848
GGTATAT453.9573206E-666.182991
GTATCAA10300.058.4080961
AGATAGT350.007780574751.0183266
GTTGACA350.007789526651.003499
GATGACC1207.2759576E-1249.5867239
ATCAACG13850.040.8566473
TCAACGC13950.040.563774
GTACAGG600.001300267439.7097971
CAACGCA14400.039.296155
GGGATGA2000.038.6720247
GTACATG24950.037.7203251
GTAGATA957.438646E-637.592456
AACGCAG15200.036.8092736
GTACAAG1503.6616257E-935.7388151
TGGGTAT854.4176522E-735.0125776
TACAAGG1209.1848597E-734.746072
TATCAAC17150.034.73162
Produced by FastQC (version 0.11.5)