##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940096_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 344090 Sequences flagged as poor quality 0 Sequence length 125 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.740041849516114 33.0 31.0 34.0 26.0 34.0 2 30.82381934958877 34.0 31.0 34.0 26.0 34.0 3 31.01222935859804 34.0 31.0 34.0 26.0 34.0 4 33.90644017553547 37.0 35.0 37.0 27.0 37.0 5 34.202859716934526 37.0 35.0 37.0 30.0 37.0 6 33.91378999680316 37.0 35.0 37.0 28.0 37.0 7 33.907559068848265 37.0 35.0 37.0 29.0 37.0 8 33.773518556191696 37.0 35.0 37.0 27.0 37.0 9 35.15685431137202 39.0 37.0 39.0 22.0 39.0 10-11 35.331855037926125 39.0 37.0 39.0 27.0 39.0 12-13 35.26978406812171 39.0 37.0 39.0 26.0 39.0 14-15 36.6753756284693 41.0 38.0 41.0 26.5 41.0 16-17 36.82579267052225 41.0 38.0 41.0 27.0 41.0 18-19 36.94919061873347 41.0 38.5 41.0 28.0 41.0 20-21 37.064180882908545 41.0 38.5 41.0 28.5 41.0 22-23 37.17794762998053 41.0 38.0 41.0 29.0 41.0 24-25 37.32709174925165 41.0 38.0 41.0 30.0 41.0 26-27 37.35870121189224 41.0 38.0 41.0 30.0 41.0 28-29 37.27592345026011 41.0 38.0 41.0 30.0 41.0 30-31 37.17679240896277 41.0 38.0 41.0 30.0 41.0 32-33 37.05161730942486 40.5 38.0 41.0 30.0 41.0 34-35 36.92376413147723 40.0 38.0 41.0 30.0 41.0 36-37 36.75158534104449 40.0 38.0 41.0 30.0 41.0 38-39 36.556995262867275 40.0 38.0 41.0 29.0 41.0 40-41 36.41081548432096 40.0 37.5 41.0 29.0 41.0 42-43 36.28147287046993 40.0 37.5 41.0 28.0 41.0 44-45 36.304686273939964 40.0 38.0 41.0 28.0 41.0 46-47 36.19381847772385 40.0 37.5 41.0 28.0 41.0 48-49 36.056257083902466 40.0 37.0 41.0 27.5 41.0 50-51 35.90681071812607 40.0 37.0 41.0 27.0 41.0 52-53 35.76846900520213 40.0 37.0 41.0 27.0 41.0 54-55 35.621151442936444 40.0 36.5 41.0 26.0 41.0 56-57 35.43852480455695 40.0 36.0 41.0 25.5 41.0 58-59 35.20535179749484 40.0 35.5 41.0 24.0 41.0 60-61 35.01418088290854 40.0 35.0 41.0 22.5 41.0 62-63 34.72703071870731 39.0 35.0 41.0 19.0 41.0 64-65 34.43210497253625 39.0 35.0 41.0 12.5 41.0 66-67 34.12310877967974 39.0 35.0 41.0 7.5 41.0 68-69 33.756829608532655 37.5 35.0 40.0 2.0 41.0 70-71 33.60733238396931 37.0 35.0 40.0 2.0 41.0 72-73 33.309995931297046 37.0 35.0 39.5 2.0 41.0 74-75 32.90902234880409 36.5 35.0 39.0 2.0 41.0 76-77 32.517241128774444 36.0 34.0 39.0 2.0 40.5 78-79 32.13246389026126 35.5 34.0 37.0 2.0 39.5 80-81 31.763153535412243 35.0 34.0 37.0 2.0 39.0 82-83 31.412066610479815 35.0 34.0 36.5 2.0 39.0 84-85 31.058776773518556 35.0 34.0 36.0 2.0 37.5 86-87 30.803371211020373 35.0 34.0 36.0 2.0 37.0 88-89 30.572406928419888 35.0 33.5 36.0 2.0 37.0 90-91 30.388380946845302 35.0 33.0 35.0 2.0 36.0 92-93 30.194742654538057 35.0 33.0 35.0 2.0 36.0 94-95 30.046595367491065 35.0 33.0 35.0 2.0 36.0 96-97 29.887131273794644 35.0 33.0 35.0 2.0 36.0 98-99 29.782433374989104 35.0 33.0 35.0 2.0 36.0 100-101 29.702958528292015 35.0 32.5 35.0 2.0 35.5 102-103 28.99038042372635 34.5 30.5 35.0 2.0 35.0 104-105 29.469243511871895 35.0 32.0 35.0 2.0 35.0 106-107 29.500587055712167 35.0 32.0 35.0 2.0 35.0 108-109 29.43859891307507 35.0 32.0 35.0 2.0 35.0 110-111 29.331503385742103 35.0 32.0 35.0 2.0 35.0 112-113 29.214846406463426 35.0 32.0 35.0 2.0 35.0 114-115 29.057714551425498 35.0 31.0 35.0 2.0 35.0 116-117 28.923973379057806 35.0 31.0 35.0 2.0 35.0 118-119 28.72446017030428 35.0 31.0 35.0 2.0 35.0 120-121 28.4644962073876 35.0 30.5 35.0 2.0 35.0 122-123 28.171135458746257 35.0 30.0 35.0 2.0 35.0 124-125 26.1174750791944 33.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.117979369425953 1101 2 0.06730654968886896 1101 3 0.15496492872730983 1101 4 0.11927370508141166 1101 5 0.12489829687024212 1101 6 -0.09277155537019155 1101 7 -0.19797097223249693 1101 8 -0.032690952681576846 1101 9 -0.040092804974342755 1101 10-11 0.0091437627038502 1101 12-13 0.13708394122846101 1101 14-15 0.23101375821578785 1101 16-17 0.13545652914746142 1101 18-19 0.42712534745950137 1101 20-21 0.2428237098126047 1101 22-23 0.050424456480556046 1101 24-25 -0.05067196357175874 1101 26-27 0.002258380991008835 1101 28-29 -0.2398114973454284 1101 30-31 -0.2570414669837646 1101 32-33 -0.4148684020075848 1101 34-35 -0.3615039366306476 1101 36-37 -0.07861693616348475 1101 38-39 -0.12646535967896 1101 40-41 -0.019863705357138883 1101 42-43 0.04878062347864898 1101 44-45 0.06569529144810815 1101 46-47 0.02866530925474109 1101 48-49 -0.1946452808717325 1101 50-51 -0.12379978337851583 1101 52-53 -0.08746005396847778 1101 54-55 -0.20053246679047732 1101 56-57 -0.24449472997817168 1101 58-59 -0.23000780690467337 1101 60-61 -0.4284848304363891 1101 62-63 -0.6982095151151313 1101 64-65 -0.522389962059485 1101 66-67 -0.407070413290171 1101 68-69 -0.49378063661124116 1101 70-71 -0.42986416658968807 1101 72-73 -0.5344762682633188 1101 74-75 -0.4337161055386005 1101 76-77 -0.33810384981259745 1101 78-79 -0.38210105630601277 1101 80-81 -0.4445858415283297 1101 82-83 -0.32755875912719645 1101 84-85 -0.26910793391889953 1101 86-87 -0.15326822965369402 1101 88-89 -0.3179428091010479 1101 90-91 -0.1991124724592126 1101 92-93 -0.1721176349039375 1101 94-95 -0.15633422424938104 1101 96-97 -0.06109739783720869 1101 98-99 -0.11581861652302194 1101 100-101 -0.034078535803256926 1101 102-103 -0.04861218430065861 1101 104-105 -0.1107134755238981 1101 106-107 -0.06280060636343876 1101 108-109 -0.12420160503422295 1101 110-111 -0.289287917439637 1101 112-113 -0.13344731564295742 1101 114-115 -0.25657507556878656 1101 116-117 -0.41845053286176537 1101 118-119 -0.26519344199039807 1101 120-121 -0.28439610765246925 1101 122-123 -0.31780080328091387 1101 124-125 0.047597843078275304 1102 1 0.3929384528956774 1102 2 0.5796848768488303 1102 3 0.5425693735545991 1102 4 0.6148116497794902 1102 5 0.39320736741895956 1102 6 0.9819291079083001 1102 7 0.5610694274573973 1102 8 0.5133422973830264 1102 9 0.6533260941556378 1102 10-11 0.741820995885 1102 12-13 0.5904477107176547 1102 14-15 0.6927847979263575 1102 16-17 0.5289874103603154 1102 18-19 0.6363767903055688 1102 20-21 0.6344810446390312 1102 22-23 0.7143619187942747 1102 24-25 0.6756136768796068 1102 26-27 0.5621041902769122 1102 28-29 0.6759305993037188 1102 30-31 0.7978857446623735 1102 32-33 0.6751135085790239 1102 34-35 0.8342924282586637 1102 36-37 0.9304751475646498 1102 38-39 0.8844141303718658 1102 40-41 1.0048154229054163 1102 42-43 0.90317376672958 1102 44-45 0.7520808543734177 1102 46-47 0.9057735873148687 1102 48-49 0.7633872800723651 1102 50-51 0.8055653643516933 1102 52-53 0.8142412122904545 1102 54-55 0.7851252125962134 1102 56-57 0.7755974398900349 1102 58-59 0.6338521293516806 1102 60-61 0.7040631926491443 1102 62-63 0.6724942492056485 1102 64-65 0.926873540395512 1102 66-67 0.894376739780725 1102 68-69 0.821535066722376 1102 70-71 0.9260751908054345 1102 72-73 0.9638095444681838 1102 74-75 0.8372934560084815 1102 76-77 0.8525456462680268 1102 78-79 0.9034187989786844 1102 80-81 0.9271085335255087 1102 82-83 0.7694091106290237 1102 84-85 0.8197843048613542 1102 86-87 0.7934239918985497 1102 88-89 0.5924629105991812 1102 90-91 0.5784049709036978 1102 92-93 0.7462818827117026 1102 94-95 0.7628448609431402 1102 96-97 0.6866500394958948 1102 98-99 0.7290388216714803 1102 100-101 0.6535345230304088 1102 102-103 0.5476278019169314 1102 104-105 0.6102616299976802 1102 106-107 0.5356704075051155 1102 108-109 0.6793473655920153 1102 110-111 0.5620903235802608 1102 112-113 0.7814161523560088 1102 114-115 0.724431040375741 1102 116-117 0.6733575470762183 1102 118-119 0.7413058258190617 1102 120-121 0.682599326924155 1102 122-123 0.3770350998976575 1102 124-125 0.5590157085274932 1103 1 -0.1539390050247249 1103 2 -0.14237451892706332 1103 3 0.05314619314013669 1103 4 -0.21643529722464194 1103 5 -0.16529010325158566 1103 6 -0.15231991720285265 1103 7 -0.34458368597744027 1103 8 -0.30194213834054295 1103 9 0.11135826755752731 1103 10-11 -0.029676234685524605 1103 12-13 -0.18327458463848245 1103 14-15 -0.0768268196026014 1103 16-17 -0.40112485709454404 1103 18-19 -0.0030012678648674296 1103 20-21 0.030025119550238344 1103 22-23 -0.3347820004658857 1103 24-25 -0.0727904859578743 1103 26-27 -0.023456277669744452 1103 28-29 0.029330912261670505 1103 30-31 0.2425364819350051 1103 32-33 0.2603676420249599 1103 34-35 0.20595897073566505 1103 36-37 0.012030498897210862 1103 38-39 0.07267530951131107 1103 40-41 -0.13944121917169383 1103 42-43 -0.07265478530175074 1103 44-45 0.00604250460565936 1103 46-47 -0.10451138656390668 1103 48-49 -0.10448733804442156 1103 50-51 0.0010316137410910642 1103 52-53 0.10228671538278178 1103 54-55 0.0397864645935897 1103 56-57 -0.0028378531561656928 1103 58-59 0.07322066631465418 1103 60-61 -0.0661300464656307 1103 62-63 0.02285605508892985 1103 64-65 0.1613001567239607 1103 66-67 0.05870864079328442 1103 68-69 0.21553711999146685 1103 70-71 0.3185890379527976 1103 72-73 0.059330023343513005 1103 74-75 0.3773094350783808 1103 76-77 0.12687026148705627 1103 78-79 0.34198631261885026 1103 80-81 0.1953871820108084 1103 82-83 0.13180903445936565 1103 84-85 0.19262029999311991 1103 86-87 0.010855252920382696 1103 88-89 0.07333770258149741 1103 90-91 0.029737112016270828 1103 92-93 0.07235219061927367 1103 94-95 0.09076145516583622 1103 96-97 -0.11372542725025525 1103 98-99 -0.24462469057775849 1103 100-101 -0.04456454720298453 1103 102-103 0.05387661176600034 1103 104-105 0.007476055868870901 1103 106-107 -0.055238495237823315 1103 108-109 -0.05892292078934602 1103 110-111 0.06607474191728002 1103 112-113 0.0910251205055026 1103 114-115 0.1165267823261793 1103 116-117 0.23609195506409364 1103 118-119 0.28563325600377354 1103 120-121 0.2370523542541605 1103 122-123 0.06309812201169507 1103 124-125 0.3210869437178765 1104 1 -0.18383230110280024 1104 2 0.016895763929440477 1104 3 0.010136616108813001 1104 4 -0.11299930945408221 1104 5 0.018220395037509718 1104 6 0.13722633803617157 1104 7 0.23493402039441946 1104 8 -0.15778048291119973 1104 9 0.32708646614425874 1104 10-11 -0.020601339465940782 1104 12-13 0.26048819654761246 1104 14-15 0.06476138829794564 1104 16-17 0.014759246653255786 1104 18-19 0.002204344720929896 1104 20-21 0.09977546881828658 1104 22-23 0.157270885804337 1104 24-25 -0.08788494018857307 1104 26-27 -0.12849788612506075 1104 28-29 0.005459199937064341 1104 30-31 0.14173670870614785 1104 32-33 0.12834836647776626 1104 34-35 0.21995803935418223 1104 36-37 0.36228257931998087 1104 38-39 0.1427678402367718 1104 40-41 0.24875858240355342 1104 42-43 0.10554278905691206 1104 44-45 0.07601760027473858 1104 46-47 0.09382225048077686 1104 48-49 0.1177625556566042 1104 50-51 0.11866914436532028 1104 52-53 0.060222693029622576 1104 54-55 0.004007242499596941 1104 56-57 0.283796822581337 1104 58-59 0.2259854519956832 1104 60-61 0.3566847503514765 1104 62-63 0.27740666352400467 1104 64-65 0.2237128397100534 1104 66-67 0.3920237514454499 1104 68-69 0.3792233659166726 1104 70-71 0.38574569226853583 1104 72-73 0.45061958174721184 1104 74-75 0.5969980297047144 1104 76-77 0.4156774857677661 1104 78-79 0.503595172891373 1104 80-81 0.5033180978017775 1104 82-83 0.594023292695006 1104 84-85 0.47919625820243894 1104 86-87 0.669240723369267 1104 88-89 0.5688488487881429 1104 90-91 0.6381215700939791 1104 92-93 0.6536354723216782 1104 94-95 0.7242851142802174 1104 96-97 0.8524795981602615 1104 98-99 1.0355085374689708 1104 100-101 0.9563364631434474 1104 102-103 0.91570211947014 1104 104-105 0.9399185025675258 1104 106-107 1.0404224819028371 1104 108-109 0.8643595915109614 1104 110-111 0.6120181706159791 1104 112-113 0.6907679782268552 1104 114-115 0.8546952003949784 1104 116-117 0.7775364062576813 1104 118-119 0.9536079062295322 1104 120-121 0.9917467193961969 1104 122-123 1.0681903490258087 1104 124-125 1.1175578177991525 1105 1 -0.2454922546733087 1105 2 -0.181663731298638 1105 3 -0.13613215906529774 1105 4 -0.08562971878760095 1105 5 -0.1857400010020953 1105 6 0.053367531313810446 1105 7 0.09586776907990924 1105 8 0.2742122153566271 1105 9 0.4669979723249327 1105 10-11 0.10206577694509633 1105 12-13 -0.05814907800148461 1105 14-15 0.029137665605027507 1105 16-17 -0.11665407503963365 1105 18-19 0.05452482727018548 1105 20-21 -0.03169340953247257 1105 22-23 0.1693346172275838 1105 24-25 0.32184541160411584 1105 26-27 0.16298408772362194 1105 28-29 0.33462247698621184 1105 30-31 0.136694942004425 1105 32-33 0.15970414734643157 1105 34-35 0.18550497480925543 1105 36-37 0.07199705778185006 1105 38-39 0.1638535760648665 1105 40-41 0.32754387576255084 1105 42-43 0.21616006320917336 1105 44-45 0.3248519025426333 1105 46-47 0.14430936332032474 1105 48-49 0.10084356916878079 1105 50-51 0.25229282737490166 1105 52-53 0.23838098982123057 1105 54-55 0.21192009791339927 1105 56-57 0.15767130421788522 1105 58-59 0.10609418431180018 1105 60-61 0.07405215843497359 1105 62-63 0.0014984113761400408 1105 64-65 0.046844251047581054 1105 66-67 -0.15844550389691392 1105 68-69 -0.13370943036896676 1105 70-71 0.009363224362424205 1105 72-73 -0.1929229488585591 1105 74-75 0.004492226018129486 1105 76-77 -0.04553999870613268 1105 78-79 -0.06744195086108107 1105 80-81 -0.010142486733634826 1105 82-83 -0.04865414138106772 1105 84-85 0.239991115243253 1105 86-87 0.19457852769078343 1105 88-89 0.14326897617162615 1105 90-91 0.21527587871121412 1105 92-93 0.25840937656580465 1105 94-95 0.22105885979103945 1105 96-97 0.30264924438339946 1105 98-99 0.4627491573225413 1105 100-101 0.34565966400061043 1105 102-103 0.14972065158387693 1105 104-105 0.13583707238042209 1105 106-107 0.1247574885497933 1105 108-109 0.2565300710839722 1105 110-111 0.22151240661272098 1105 112-113 0.054164077355807194 1105 114-115 -0.07664073881016265 1105 116-117 -0.01838017938885983 1105 118-119 0.0195100074616974 1105 120-121 0.12723353461078446 1105 122-123 0.16564267942918676 1105 124-125 0.23806814538501797 1106 1 -0.2009737698509042 1106 2 -0.3243786089066134 1106 3 -0.46832619949789844 1106 4 -0.11009582892197756 1106 5 -0.48635349793460847 1106 6 -0.5981276881639985 1106 7 -0.5429476941573981 1106 8 -0.17515277228737602 1106 9 -0.561443368434297 1106 10-11 -0.3691445373440132 1106 12-13 -0.41844840876936473 1106 14-15 -0.4704873512999441 1106 16-17 -0.3592025667059886 1106 18-19 -0.4232466666378798 1106 20-21 -0.32273561664607797 1106 22-23 -0.18958772643725297 1106 24-25 -0.3437516311521449 1106 26-27 -0.45392662531800454 1106 28-29 -0.7191353454070395 1106 30-31 -0.4650299802326856 1106 32-33 -0.3930065911038838 1106 34-35 -0.3390308043154562 1106 36-37 -0.36833759926230414 1106 38-39 -0.38028992566250963 1106 40-41 -0.15682363225963059 1106 42-43 -0.16672431495410223 1106 44-45 -0.14119675919802432 1106 46-47 -0.18631097756595238 1106 48-49 -0.20571433190945498 1106 50-51 -0.07156552327322174 1106 52-53 -0.21264170997509524 1106 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2216 78-79 -0.7629970220424056 2216 80-81 -0.6667121195056716 2216 82-83 -0.6241151282899473 2216 84-85 -0.6434136813629472 2216 86-87 -0.7185935957769303 2216 88-89 -0.7026761608556775 2216 90-91 -0.7076576506852419 2216 92-93 -0.6904721527265423 2216 94-95 -0.5455474046859656 2216 96-97 -0.6669864555879705 2216 98-99 -0.6094943556774837 2216 100-101 -0.7153958886631635 2216 102-103 -0.5471096974258316 2216 104-105 -0.48601051890604907 2216 106-107 -0.6909713717682457 2216 108-109 -0.6515848600770511 2216 110-111 -0.6417091664034409 2216 112-113 -0.6049967768200766 2216 114-115 -0.5631169379617127 2216 116-117 -0.5909636580344646 2216 118-119 -0.9617739891028627 2216 120-121 -0.9047573953009902 2216 122-123 -0.9210648264917438 2216 124-125 -1.2249601840010271 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8518.0 3 1067.0 4 951.0 5 911.0 6 2538.0 7 2921.0 8 2995.0 9 2738.0 10 2687.0 11 2558.0 12 2159.0 13 1873.0 14 1625.0 15 1566.0 16 1332.0 17 1146.0 18 1004.0 19 910.0 20 836.0 21 770.0 22 851.0 23 1208.0 24 1894.0 25 2080.0 26 2671.0 27 2204.0 28 2237.0 29 3031.0 30 3672.0 31 4162.0 32 5540.0 33 7956.0 34 11671.0 35 18567.0 36 38168.0 37 117334.0 38 79674.0 39 65.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.740768624806336 24.109855036233675 14.533002729516273 21.61637360944372 2 14.143208010580011 24.930459678239767 40.25607115348283 20.670261157697396 3 15.115200944046087 34.523370081761136 29.470921462225075 20.8905075119677 4 10.7163312119688 21.04889798552743 41.18257593157364 27.052194870930137 5 10.667988475839675 42.972268172211194 34.75447200797737 11.605271343971765 6 24.986119791288168 43.60304056509164 18.977951542810633 12.432888100809558 7 24.913822669433642 36.170645646954874 25.188485662301126 13.72704602131036 8 23.461867091366692 37.60484297530269 22.4153264009579 16.517963532372722 9 26.455961470828456 19.19046885490814 21.33230247841163 33.02126719585178 10-11 20.821066642641874 32.15112950428523 28.960966494133782 18.06683735893911 12-13 22.703104088165926 30.088046768397327 28.27697749205487 18.93187165138188 14-15 21.73714637825646 31.491336939429786 25.39900071792517 21.372515964388587 16-17 19.304007161042072 34.095889375850085 27.24305692795945 19.357046535148395 18-19 19.38818366116129 32.72810504058188 29.01855067830232 18.865160619954512 20-21 21.4237760042781 31.98983951313365 27.936398142281693 18.649986340306558 22-23 21.79691359818652 31.600889302217443 27.658461449039496 18.94373565055654 24-25 20.74839422210597 32.14389513761734 28.081931002412304 19.02577963786439 26-27 19.454095518753114 32.716342695723064 27.921950539031272 19.907611246492554 28-29 20.243670991846148 30.83176267654449 29.302279620019732 19.62228671158963 30-31 20.537166123920503 32.117930676710735 28.271044673749252 19.073858525619507 32-33 19.382298405612616 32.828947750826835 28.218709493667205 19.57004434989334 34-35 19.865150579431106 32.05231227522069 28.643295673338905 19.4392414720093 36-37 19.871652345265655 31.867914684849254 27.12434115015891 21.136091819726186 38-39 19.050034003127124 32.71609423225589 28.65097679068605 19.582894973930937 40-41 19.07669860019532 32.23431032879133 29.18546249360554 19.503528577407803 42-43 19.11956613353717 31.751189441652695 28.273979928444586 20.855264496365557 44-45 18.963490391252225 32.0768699821993 28.253714534820357 20.705925091728123 46-47 19.347605255547435 31.777867233629458 28.37521853305644 20.49930897776667 48-49 18.930618828606928 32.218177590804395 28.002266931141968 20.84893664944671 50-51 19.414487403994972 31.90353356633702 27.889378955552484 20.79260007411552 52-53 19.034872805373183 32.04918056609093 28.43126142542846 20.484685203107425 54-55 19.7522109491024 31.793927627926983 28.022017943345062 20.431843479625556 56-57 19.909376008184644 32.05642055577677 27.071810358107186 20.962393077931402 58-59 18.477491552519712 31.33161355956836 28.934491152853976 21.25640373505795 60-61 19.51964868788354 32.483001846946145 26.98706556440843 21.010283900761884 62-63 19.51898623806548 32.17982067341927 28.180866987342508 20.120326101172743 64-65 19.65880523707805 31.147536219248153 28.27629800775971 20.91736053591409 66-67 19.26553245409578 32.19151607446975 27.153822009613876 21.38912946182059 68-69 19.700483597219314 31.63870870242496 27.834739950245286 20.826067750110436 70-71 20.281220209723546 30.77321025831803 27.97655723220721 20.969012299751213 72-73 19.448860317303474 31.36575924112216 27.356017518825183 21.829362922749187 74-75 19.75199741912233 31.802051332726865 27.142084872511035 21.30386637563977 76-77 19.174656046132764 31.505839985699772 28.79097690600653 20.528527062160933 78-79 19.469317174535362 30.763038202769664 28.515919031634624 21.25172559106035 80-81 19.933154108842547 32.396134563685244 26.870449756593768 20.80026157087844 82-83 20.64870693100096 30.406339140530886 27.693488082142615 21.25146584632554 84-85 19.93841718506377 29.73514158380305 28.336086977370712 21.990354253762465 86-87 20.05146991509353 30.780229856328894 28.132479085805954 21.035821142771617 88-89 20.51119594391347 29.589000088643335 28.905283869383318 20.994520098059873 90-91 20.55009314992051 29.648994823712687 28.44749163685191 21.353420389514895 92-93 21.354464136492783 29.91621796408926 27.73265319468969 20.99666470472827 94-95 20.32564154726961 29.09267924089628 28.913511000029064 21.66816821180505 96-97 20.705368806662218 29.558007437018567 28.037327497642327 21.699296258676885 98-99 19.90548805740467 30.108534701605862 28.337888913592195 21.648088327397275 100-101 19.61172948937778 30.110145601441484 28.558952599610564 21.71917230957017 102-103 20.53582067430229 30.34302171821825 27.73134276306421 21.389814844415252 104-105 18.686802630928817 32.322756720465264 27.86584355681115 21.12459709179477 106-107 19.130605364875468 31.786596529977622 27.838937487285303 21.243860617861607 108-109 19.28260048243192 31.93190734982127 26.917230957017058 21.86826121072975 110-111 18.85750322954705 33.53439594755419 26.486499839430845 21.121600983467918 112-113 18.515881239110122 33.43713988016956 27.686848151318266 20.360130729402055 114-115 18.756947865397777 33.62730523816374 26.981912662879765 20.63383423355872 116-117 18.700539685953345 33.817268206772084 26.674930032636908 20.807262074637666 118-119 18.623900672529746 32.8375581996896 26.914704394984803 21.62383673279585 120-121 18.47334234273508 33.30732231861313 26.645305665751195 21.57402967290059 122-123 18.91676281427189 33.26014490437067 26.642380314395403 21.180711966962036 124-125 19.119221014914263 33.549233346518065 26.075670676376834 21.255874962190838 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1036.0 1 808.5 2 644.5 3 603.0 4 402.5 5 272.0 6 212.5 7 198.0 8 216.0 9 222.5 10 177.0 11 124.0 12 116.5 13 136.0 14 150.5 15 146.5 16 151.5 17 177.5 18 209.0 19 250.0 20 312.5 21 376.5 22 439.0 23 539.5 24 662.0 25 792.5 26 1027.5 27 1372.0 28 1690.0 29 2370.5 30 3625.0 31 4907.5 32 7391.5 33 10528.5 34 12495.0 35 14983.5 36 20658.0 37 24994.0 38 24501.0 39 23359.5 40 21723.0 41 18522.5 42 14583.5 43 13786.0 44 14848.5 45 14409.5 46 11977.0 47 9500.0 48 8339.5 49 6867.0 50 5856.5 51 4598.5 52 3718.0 53 3450.0 54 3200.0 55 3090.0 56 3083.5 57 2866.0 58 2498.5 59 2299.0 60 2276.0 61 2317.5 62 1991.5 63 1148.5 64 580.0 65 644.5 66 676.0 67 471.5 68 303.0 69 206.0 70 195.5 71 197.5 72 181.0 73 224.0 74 209.0 75 129.0 76 75.0 77 56.0 78 49.0 79 28.0 80 16.5 81 16.0 82 12.5 83 5.0 84 5.0 85 6.0 86 5.0 87 4.5 88 3.5 89 3.0 90 3.0 91 3.0 92 4.5 93 4.0 94 3.0 95 4.0 96 4.5 97 5.0 98 5.5 99 7.5 100 18.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02121537969717225 2 0.0130779737859281 3 0.011334243947804353 4 0.03749019151966056 5 0.034002731843413056 6 0.021796622976546835 7 0.009299892469993316 8 0.0011624865587491645 9 0.011334243947804353 10-11 2.906216396872911E-4 12-13 0.003342148856403848 14-15 0.012496730506553517 16-17 0.0017437298381237465 18-19 0.009445203289836961 20-21 0.003487459676247493 22-23 0.0 24-25 0.005812432793745822 26-27 1.4531081984364556E-4 28-29 0.005667121973902177 30-31 0.0018890406579673924 32-33 0.002324973117498329 34-35 7.265540992182277E-4 36-37 0.007120230172338632 38-39 0.0011624865587491645 40-41 0.012206108866866227 42-43 0.006103054433433113 44-45 7.265540992182277E-4 46-47 0.010026446569211544 48-49 0.003632770496091139 50-51 0.009445203289836961 52-53 0.003196838036560202 54-55 0.002034351477811038 56-57 0.009009270830306025 58-59 0.015257636083582785 60-61 0.002470283937341974 62-63 0.007265540992182278 64-65 0.0014531081984364555 66-67 0.0011624865587491645 68-69 5.812432793745822E-4 70-71 0.005231189514371241 72-73 8.718649190618732E-4 74-75 0.006103054433433113 76-77 0.011479554767647998 78-79 0.0014531081984364555 80-81 0.004359324595309366 82-83 0.001598419018280101 84-85 1.4531081984364556E-4 86-87 0.001598419018280101 88-89 0.004214013775465721 90-91 0.006103054433433113 92-93 0.012351419686709874 94-95 0.0 96-97 1.4531081984364556E-4 98-99 0.0018890406579673924 100-101 0.0 102-103 2.906216396872911E-4 104-105 0.008428027550931442 106-107 0.0 108-109 0.0 110-111 4.359324595309366E-4 112-113 0.005957743613589468 114-115 0.0027609055770292656 116-117 2.906216396872911E-4 118-119 0.004649946234996658 120-121 0.0014531081984364555 122-123 2.906216396872911E-4 124-125 0.07498038303932111 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 344090.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.97061815222762 #Duplication Level Percentage of deduplicated Percentage of total 1 75.85795255053418 21.97651777151327 2 9.256156894216783 5.36313173878927 3 3.8180267843707676 3.31831788194949 4 2.225008777649596 2.5783951873056465 5 1.4064302553042083 2.0372576942079106 6 0.9600240758388926 1.6687494550844257 7 0.7303004464061794 1.4810078758464356 8 0.610924411897477 1.4159086285564824 9 0.4805136178963736 1.2528698886919118 >10 3.6444801123539152 21.36359673341277 >50 0.5738074936048553 11.623993722572584 >100 0.420324020665095 22.600482431921883 >500 0.015047399307819632 2.8661106105960648 >1k 0.001003159953854642 0.45366037955186145 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1561 0.45366037955186145 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 911 0.2647563137551222 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 868 0.2522595832485687 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 843 0.2449940422563864 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 805 0.23395041994826934 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 716 0.20808509401610045 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 709 0.2060507425382894 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 706 0.2051788776192275 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 679 0.19733209334767068 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 576 0.1673980644598797 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 523 0.15199511755645326 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 513 0.14908890115958034 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 506 0.14705454968176931 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 504 0.14647330640239473 No Hit ACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTT 502 0.14589206312302014 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 501 0.14560144148333284 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 494 0.1435670900055218 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 492 0.14298584672614723 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 481 0.13978900868958702 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 476 0.13833590049115058 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 472 0.1371734139324014 No Hit ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC 468 0.13601092737365222 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 465 0.13513906245459037 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 448 0.13019849457990643 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 447 0.12990787294021913 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 443 0.12874538638146998 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 433 0.12583916998459704 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 420 0.12206108866866228 No Hit TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA 420 0.12206108866866228 No Hit CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC 411 0.11944549391147664 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 407 0.1182830073527275 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 405 0.1177017640733529 No Hit TATATAATCCCAACAAACGACCTAAAACCTGGTGAACTACGACTGCTAGA 404 0.11741114243366561 No Hit CTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCT 401 0.11653927751460373 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 395 0.11479554767648 No Hit CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC 390 0.11334243947804354 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 389 0.11305181783835626 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 378 0.10985497980179604 No Hit GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT 378 0.10985497980179604 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 367 0.10665814176523584 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 366 0.10636752012554854 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 359 0.10433316864773752 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 355 0.10317068208898834 No Hit CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG 355 0.10317068208898834 No Hit GCTATGGTTAGATCCACAAATTTCAGAGCATTGGCCATAGAATAACCCTG 355 0.10317068208898834 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 352 0.10229881716992646 No Hit CCCTAGGACTTAAAACTGATGCCATCCCAGGCCGACTAAATCAAGCAACA 350 0.1017175738905519 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.812432793745822E-4 0.0 18-19 0.0 0.0 0.0 0.0011624865587491645 0.0 20-21 0.0 0.0 0.0 0.0014531081984364555 0.0 22-23 0.0 0.0 0.0 0.004359324595309366 0.0 24-25 0.0 0.0 0.0 0.011043622308117063 0.0 26-27 0.0 0.0 0.0 0.024702839373419744 0.0 28-29 0.0 0.0 0.0 0.046354151530122935 0.0 30-31 0.0 0.0 0.0 0.10389723618820657 0.0 32-33 0.0 0.0 0.0 0.19791333662704524 0.0 34-35 0.0 0.0 0.0 0.3220087767735186 0.0 36-37 0.0 0.0 0.0 0.5222470865180622 0.0 38-39 0.0 0.0 0.0 0.8170827399808189 0.0 40-41 0.0 0.0 0.0 1.1575459908744805 0.0 42-43 0.0 0.0 0.0 1.4551425499142665 0.0 44-45 0.0 0.0 0.0 1.7756982184893486 0.0 46-47 0.0 0.0 0.0 2.1401377546572116 0.0 48-49 0.0 0.0 0.0 2.5314597924961495 0.0 50-51 0.0 0.0 0.0 2.963032927431777 0.0 52-53 0.0 0.0 0.0 3.361329884623209 0.0 54-55 0.0 0.0 0.0 3.7420442326135603 0.0 56-57 0.0 0.0 0.0 4.160830015402947 0.0 58-59 0.0 0.0 0.0 4.663896073701648 0.0 60-61 0.0 0.0 0.0 5.19529774186986 0.0 62-63 0.0 0.0 0.0 5.711005841494957 0.0 64-65 0.0 0.0 0.0 6.209421953558662 0.0 66-67 0.0 0.0 0.0 6.760295271585923 0.0 68-69 0.0 0.0 0.0 7.335435496527071 0.0 70-71 0.0 0.0 0.0 7.998343456653783 0.0 72-73 0.0 0.0 0.0 8.65558429480659 0.0 74-75 0.0 0.0 0.0 9.305995524426748 0.0 76-77 0.0 0.0 0.0 9.979511174402045 0.0 78-79 0.0 0.0 0.0 10.746025749077276 0.0 80-81 0.0 0.0 0.0 11.536080676567178 0.0 82-83 0.0 0.0 0.0 12.386584905112034 0.0 84-85 0.0 0.0 0.0 13.24173907989189 0.0 86-87 0.0 0.0 0.0 14.088319916300968 0.0 88-89 0.0 0.0 0.0 14.950449010433317 0.0 90-91 0.0 0.0 0.0 15.955999883751344 0.0 92-93 0.0 1.4531081984364556E-4 0.0 17.039873288965097 0.0 94-95 0.0 2.906216396872911E-4 0.0 18.117934261385102 0.0 96-97 0.0 2.906216396872911E-4 0.0 19.199046761021826 0.0 98-99 0.0 2.906216396872911E-4 0.0 20.281902990496672 0.0 100-101 0.0 2.906216396872911E-4 0.0 21.463861199104883 0.0 102-103 0.0 2.906216396872911E-4 0.0 22.736638670115376 0.0 104-105 0.0 2.906216396872911E-4 0.0 23.999970937836032 0.0 106-107 0.0 2.906216396872911E-4 0.0 25.282920166235577 0.0 108-109 0.0 2.906216396872911E-4 0.0 26.50745444505798 0.0 110-111 0.0 2.906216396872911E-4 0.0 27.807840971838765 0.0 112-113 0.0 2.906216396872911E-4 0.0 29.124066377982505 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 500 0.0 77.434105 1 AGTTGAC 25 0.0020550608 71.404884 8 GCATCGA 25 0.0020550608 71.404884 8 GGTATAT 45 3.9573206E-6 66.18299 1 GTATCAA 1030 0.0 58.408096 1 AGATAGT 35 0.0077805747 51.018326 6 GTTGACA 35 0.0077895266 51.00349 9 GATGACC 120 7.2759576E-12 49.586723 9 ATCAACG 1385 0.0 40.856647 3 TCAACGC 1395 0.0 40.56377 4 GTACAGG 60 0.0013002674 39.709797 1 CAACGCA 1440 0.0 39.29615 5 GGGATGA 200 0.0 38.672024 7 GTACATG 2495 0.0 37.720325 1 GTAGATA 95 7.438646E-6 37.59245 6 AACGCAG 1520 0.0 36.809273 6 GTACAAG 150 3.6616257E-9 35.738815 1 TGGGTAT 85 4.4176522E-7 35.012577 6 TACAAGG 120 9.1848597E-7 34.74607 2 TATCAAC 1715 0.0 34.7316 2 >>END_MODULE