FastQCFastQC Report
Fri 27 May 2016
SRR940096_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940096_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344090
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29590.8599494318346943No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24960.7253916126594786No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT20880.6068179836670639No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14290.41529832311313897No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT10590.3077683164288413RNA PCR Primer, Index 31 (95% over 22bp)
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA8810.2560376645645035No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC8290.24092533930076435No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG7910.22988171699264728No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT7600.22087244616234128RNA PCR Primer, Index 31 (95% over 24bp)
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT7260.21099131041297337No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT7030.20430701270016566No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG7020.20401639106047836No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA6490.18861344415705195No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC6360.18483536284111715No Hit
ACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTT5430.15780755035019908No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG5210.15141387427707867No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT5080.14763579296114387No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT5040.14647330640239473No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC5020.14589206312302014No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG4900.14240460344677264No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG4900.14240460344677264No Hit
CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC4870.14153273852771078No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC4820.1400796303292743No Hit
GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG4670.13572030573396496No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC4600.13368595425615393No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4600.13368595425615393No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT4590.1333953326164666No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA4560.13252346769740475No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG4480.13019849457990643No Hit
ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC4460.12961725130053184No Hit
TATATAATCCCAACAAACGACCTAAAACCTGGTGAACTACGACTGCTAGA4460.12961725130053184No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4440.12903600802115725No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4420.12845476474178266No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT4370.12700165654334622No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA4310.12525792670522248No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA4290.1246766834258479No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA4140.12031735883053851No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC4140.12031735883053851No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG4090.11886425063210207No Hit
CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC4080.11857362899241478No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA4060.11799238571304019No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4000.11624865587491645No Hit
TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA3980.11566741259554188No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA3890.11305181783835626No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA3870.11247057455898166No Hit
GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG3760.10927373652242146No Hit
CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG3730.10840187160335958No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG3730.10840187160335958No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT3660.10636752012554854No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT3620.10520503356679937No Hit
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG3590.10433316864773752RNA PCR Primer, Index 31 (96% over 29bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3570.10375192536836292No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC3550.10317068208898834No Hit
CTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCT3530.10258943880961376No Hit
GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT3510.10200819553023918No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT3490.10142695225086461No Hit
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT3460.10055508733180273RNA PCR Primer, Index 31 (96% over 27bp)
GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT3450.10026446569211545No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTGGCG152.6947417E-4119.059193
GGCGAGC152.699425E-4119.007276
GCGAGCA152.7025506E-4118.972687
GGTATCA11850.092.474481
TTGGCGA208.45977E-489.294394
AGATAGT750.079.338186
GATAGTC850.069.9839257
GTATCAA26600.066.048481
TAGTCAA1000.065.4349759
CGGAGAT300.004218403259.5555841
TAGATAG1150.056.933075
GTACAGG453.153228E-452.9382931
ATACTCG709.118485E-751.0031136
TACTCGC709.136802E-750.988297
GTTCGAC607.2759576E-1249.5719516-17
ATCAACG37550.046.6090623
TCAACGC37700.046.423614
CAACGCA38100.045.9295435
TCTAGAT1301.8189894E-1145.7919963
CGACCGT651.8189894E-1145.75872418-19
Produced by FastQC (version 0.11.5)