##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940096_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 344090 Sequences flagged as poor quality 0 Sequence length 125 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.69320526606411 34.0 31.0 34.0 31.0 34.0 2 32.8003719956988 34.0 31.0 34.0 31.0 34.0 3 32.84675811560929 34.0 31.0 34.0 31.0 34.0 4 36.28199017698858 37.0 37.0 37.0 35.0 37.0 5 36.27633177366387 37.0 37.0 37.0 35.0 37.0 6 36.34622046557587 37.0 37.0 37.0 35.0 37.0 7 36.30301084018716 37.0 37.0 37.0 35.0 37.0 8 36.31360690517016 37.0 37.0 37.0 35.0 37.0 9 38.1282571420268 39.0 39.0 39.0 37.0 39.0 10-11 38.104062890522826 39.0 39.0 39.0 36.0 39.0 12-13 38.12189834055044 39.0 39.0 39.0 37.0 39.0 14-15 39.52164985904851 41.0 39.0 41.0 36.5 41.0 16-17 39.528889244093115 41.0 39.0 41.0 36.5 41.0 18-19 39.6528844197739 41.0 40.0 41.0 37.0 41.0 20-21 39.649982562701624 41.0 40.0 41.0 37.0 41.0 22-23 39.578153971344705 41.0 40.0 41.0 36.0 41.0 24-25 39.5333648173443 41.0 40.0 41.0 36.0 41.0 26-27 39.38001249673051 41.0 39.0 41.0 35.5 41.0 28-29 39.23930367055131 41.0 39.0 41.0 35.0 41.0 30-31 38.96903426429132 41.0 38.5 41.0 35.0 41.0 32-33 38.613682466796476 40.5 38.0 41.0 35.0 41.0 34-35 38.39800052311895 40.0 38.0 41.0 35.0 41.0 36-37 38.198090615827255 40.0 38.0 41.0 35.0 41.0 38-39 37.953826033886486 40.0 38.0 41.0 34.0 41.0 40-41 37.79129733499956 40.0 37.0 41.0 34.0 41.0 42-43 37.51016449184806 40.0 37.0 41.0 33.0 41.0 44-45 37.33636548577407 40.0 38.0 41.0 33.0 41.0 46-47 36.68733470894243 41.0 38.0 41.0 30.5 41.0 48-49 36.0499070010753 40.0 37.5 41.0 25.5 41.0 50-51 35.726875236130084 40.0 37.0 41.0 23.0 41.0 52-53 35.64103868174024 40.0 37.0 41.0 23.0 41.0 54-55 35.52718184195995 40.0 36.5 41.0 23.0 41.0 56-57 35.38868319335057 40.0 36.0 41.0 22.0 41.0 58-59 35.21405736871168 40.0 36.0 41.0 21.5 41.0 60-61 35.02359847714261 40.0 35.0 41.0 19.0 41.0 62-63 34.75761138074341 39.0 35.0 41.0 16.5 41.0 64-65 34.453975704030924 39.0 35.0 41.0 11.5 41.0 66-67 34.10745589816618 39.0 35.0 41.0 6.0 41.0 68-69 33.677972332819905 37.5 35.0 40.5 2.0 41.0 70-71 33.32303321805341 37.0 35.0 40.0 2.0 41.0 72-73 33.03843616495685 37.0 35.0 39.0 2.0 41.0 74-75 32.685275654625244 36.0 35.0 39.0 2.0 41.0 76-77 32.338001685605505 36.0 34.5 39.0 2.0 40.5 78-79 31.997384405242812 35.5 34.0 37.0 2.0 39.5 80-81 31.661409805574124 35.0 34.0 37.0 2.0 39.0 82-83 31.3508369903223 35.0 34.0 36.5 2.0 39.0 84-85 31.051158127234153 35.0 34.0 36.0 2.0 37.0 86-87 30.83775175099538 35.0 34.0 36.0 2.0 37.0 88-89 30.648301316516026 35.0 34.0 35.5 2.0 37.0 90-91 30.510665814176523 35.0 34.0 35.0 2.0 36.0 92-93 30.36726873783022 35.0 34.0 35.0 2.0 36.0 94-95 30.252540033130867 35.0 33.0 35.0 2.0 36.0 96-97 30.166909820105204 35.0 33.0 35.0 2.0 36.0 98-99 30.108879944200645 35.0 33.0 35.0 2.0 35.5 100-101 30.065331744601703 35.0 33.0 35.0 2.0 35.0 102-103 29.342576070214186 34.5 31.5 35.0 2.0 35.0 104-105 29.883229678281843 35.0 33.0 35.0 2.0 35.0 106-107 29.91669040076724 35.0 33.0 35.0 2.0 35.0 108-109 29.884589787555583 35.0 33.0 35.0 2.0 35.0 110-111 29.79099363538609 35.0 33.0 35.0 2.0 35.0 112-113 29.687350693132608 35.0 33.0 35.0 2.0 35.0 114-115 29.548815716818275 35.0 33.0 35.0 2.0 35.0 116-117 29.385284373274434 35.0 33.0 35.0 2.0 35.0 118-119 29.244552297364063 35.0 33.0 35.0 2.0 35.0 120-121 29.04959022348804 35.0 33.0 35.0 2.0 35.0 122-123 28.828771542329044 35.0 32.5 35.0 2.0 35.0 124-125 27.13500101717574 33.0 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22135685012459305 1101 2 0.3069482537798933 1101 3 0.17868352208643046 1101 4 0.05331914712419206 1101 5 0.172114813248875 1101 6 0.19919799338687483 1101 7 0.1813130996009491 1101 8 0.15964493938234625 1101 9 0.27171855242193743 1101 10-11 0.1576066062431778 1101 12-13 0.15962187794144 1101 14-15 0.2268910738937322 1101 16-17 0.25937877617737115 1101 18-19 0.3049719656280345 1101 20-21 0.2247274280358269 1101 22-23 0.22032214726144872 1101 24-25 0.27936215689344124 1101 26-27 0.28762633774899626 1101 28-29 0.007433599206123631 1101 30-31 0.2122151897056952 1101 32-33 0.26681265619496486 1101 34-35 0.3322088224091857 1101 36-37 0.31108398578358987 1101 38-39 0.3219477598769416 1101 40-41 0.2734899203637511 1101 42-43 0.17639044301630236 1101 44-45 0.10359638731944898 1101 46-47 -0.04564482537813319 1101 48-49 -0.1077970331581426 1101 50-51 0.24587482605561917 1101 52-53 0.1276324218506133 1101 54-55 0.060447565522608215 1101 56-57 0.19496572262552547 1101 58-59 0.2506313001207161 1101 60-61 0.1859603064979396 1101 62-63 0.20534257173795112 1101 64-65 0.13055785956641586 1101 66-67 -0.012744649672150388 1101 68-69 0.05125481672064325 1101 70-71 0.06702557217453631 1101 72-73 0.23238397923928744 1101 74-75 0.028607126996149645 1101 76-77 0.04406769780094777 1101 78-79 0.1362462403195721 1101 80-81 0.014495674403026015 1101 82-83 0.07355160479194822 1101 84-85 0.06718671146963118 1101 86-87 0.03297186621467674 1101 88-89 0.1855242503070933 1101 90-91 0.048798662097510714 1101 92-93 0.20287145855276734 1101 94-95 0.3177372154058098 1101 96-97 0.21483994314902688 1101 98-99 0.32059337287585166 1101 100-101 0.23935260652581292 1101 102-103 0.1430250768577288 1101 104-105 0.16015916457275736 1101 106-107 0.21526593030776908 1101 108-109 0.16014435267830507 1101 110-111 0.07513672249531211 1101 112-113 0.06740291341540328 1101 114-115 0.1647776854892271 1101 116-117 0.361084516237721 1101 118-119 0.37805739517135706 1101 120-121 0.25878427191253905 1101 122-123 0.29778241455704446 1101 124-125 0.27198021196999633 1102 1 0.2637463754928433 1102 2 0.24925163908439885 1102 3 0.18845180531496197 1102 4 0.1584444809169412 1102 5 0.06133869127454261 1102 6 0.010275605584304515 1102 7 0.21359839549206328 1102 8 0.16867241806267685 1102 9 0.1884986368391992 1102 10-11 0.24932854611741107 1102 12-13 0.20001295383083573 1102 14-15 0.3740958288252543 1102 16-17 0.374647102475933 1102 18-19 0.24654746739390276 1102 20-21 0.3331863822954517 1102 22-23 0.3930760450993347 1102 24-25 0.27940953392850076 1102 26-27 0.23342700964149543 1102 28-29 0.22224538316683606 1102 30-31 0.36363650075746534 1102 32-33 0.3210076772992778 1102 34-35 0.45984255485814884 1102 36-37 0.4044899639040196 1102 38-39 0.44099763497384714 1102 40-41 0.1755055978553557 1102 42-43 0.13684784675478312 1102 44-45 0.49686884834107303 1102 46-47 0.6165095376160963 1102 48-49 0.508664333023269 1102 50-51 0.5097261483055959 1102 52-53 0.5934563515603131 1102 54-55 0.6474059598718185 1102 56-57 0.5764800649461392 1102 58-59 0.3071176469206165 1102 60-61 0.5381267291010872 1102 62-63 0.4563461034805627 1102 64-65 0.6181709184000823 1102 66-67 0.5959641107723286 1102 68-69 0.6771958969170413 1102 70-71 0.7718451087409832 1102 72-73 0.7407180304064838 1102 74-75 0.8269230887499575 1102 76-77 0.8087588856724253 1102 78-79 0.8778131280790689 1102 80-81 0.6621483576660161 1102 82-83 0.7326996795615237 1102 84-85 0.6398803112628961 1102 86-87 0.7413963644488071 1102 88-89 0.7705617212348201 1102 90-91 0.852584517899043 1102 92-93 0.8517473307209116 1102 94-95 0.9351332012788411 1102 96-97 0.8816114135599129 1102 98-99 0.8312962757959959 1102 100-101 0.6756994064224457 1102 102-103 0.5642026115291081 1102 104-105 0.7181185064626696 1102 106-107 0.6324508730246272 1102 108-109 0.6847028848516139 1102 110-111 0.7147568448142039 1102 112-113 0.7377750384907742 1102 114-115 0.5686367603449405 1102 116-117 0.39214231622049667 1102 118-119 0.3227167112192504 1102 120-121 0.5158692921554575 1102 122-123 0.49046912314520696 1102 124-125 0.28910054963904486 1103 1 -0.08429515353027739 1103 2 0.08817350722809891 1103 3 -0.07679661540649363 1103 4 0.06479719611692758 1103 5 -0.0824646585346187 1103 6 0.011977003959906085 1103 7 -0.0795057665174852 1103 8 0.040757065736606535 1103 9 -0.1434230739975888 1103 10-11 -0.02602477347225829 1103 12-13 0.17377581781003926 1103 14-15 0.1257206435756686 1103 16-17 0.14898413666120547 1103 18-19 0.1567210845420064 1103 20-21 0.20231738583071746 1103 22-23 0.06681738287037575 1103 24-25 0.1654344712127198 1103 26-27 0.22059200802703316 1103 28-29 0.154564086957933 1103 30-31 0.09009014075647315 1103 32-33 0.10177980582729873 1103 34-35 -0.08233018468301623 1103 36-37 -0.41444357806795296 1103 38-39 -0.005855398979598192 1103 40-41 0.14135965016404128 1103 42-43 -0.17983372267137554 1103 44-45 -0.026238708185921666 1103 46-47 -0.0337599539514315 1103 48-49 -0.36707563038218183 1103 50-51 -0.07484701182455211 1103 52-53 -0.02789557710513435 1103 54-55 0.16143525207353804 1103 56-57 0.08205618079026067 1103 58-59 -0.0610012108656619 1103 60-61 0.14714248462960455 1103 62-63 0.13946997024989116 1103 64-65 -0.10600177146521617 1103 66-67 0.023412509967585038 1103 68-69 -0.14850805148629576 1103 70-71 -0.08098600159738822 1103 72-73 -0.20228512401094179 1103 74-75 -0.1637502675998661 1103 76-77 -0.1467646565479157 1103 78-79 -0.2369774467534853 1103 80-81 -0.10046103272832951 1103 82-83 -0.12035911182467984 1103 84-85 -0.04824425924785203 1103 86-87 0.027541765705606736 1103 88-89 -0.04724997411965859 1103 90-91 -0.012820795762646497 1103 92-93 -0.2902821767428847 1103 94-95 -0.16629663570152786 1103 96-97 0.08863144121281508 1103 98-99 0.22737229642804024 1103 100-101 0.1892547080791367 1103 102-103 0.18994671458996848 1103 104-105 0.21396850190904715 1103 106-107 0.30871326094345264 1103 108-109 0.27632587815365284 1103 110-111 0.2177725837840896 1103 112-113 0.36551238563399835 1103 114-115 0.2689546858808214 1103 116-117 0.38545470289742667 1103 118-119 0.38019375682997136 1103 120-121 0.2766779324442368 1103 122-123 0.398119620317825 1103 124-125 0.44709877786652896 1104 1 0.03686137930091604 1104 2 -0.030970431705675594 1104 3 0.09014339147763195 1104 4 0.04388160762556481 1104 5 -0.13013924194387982 1104 6 0.06962141870813099 1104 7 -0.12477495601312683 1104 8 0.1837443706020636 1104 9 0.19301621410300385 1104 10-11 0.20660597426508787 1104 12-13 0.16303455963514324 1104 14-15 0.10875330266980399 1104 16-17 0.16230694133431456 1104 18-19 0.15632018555640315 1104 20-21 0.08167918704151589 1104 22-23 0.19325241901203327 1104 24-25 0.2132993804030221 1104 26-27 0.2629475932788097 1104 28-29 0.24345635041741076 1104 30-31 0.2649853603397787 1104 32-33 0.3654539032983948 1104 34-35 0.45661370970643134 1104 36-37 0.4526260123266752 1104 38-39 0.44817485067112983 1104 40-41 0.35458324246191353 1104 42-43 0.22612711881154723 1104 44-45 0.2102532232158083 1104 46-47 0.2485093772761786 1104 48-49 0.45702285308579604 1104 50-51 0.5146445753709799 1104 52-53 0.5111799255371494 1104 54-55 0.2941530637320042 1104 56-57 0.2529181136093044 1104 58-59 0.3635973839631461 1104 60-61 0.21202940276926796 1104 62-63 0.2696042106678007 1104 64-65 0.46404216544381427 1104 66-67 0.05685720413025308 1104 68-69 -0.11549260270215456 1104 70-71 -0.019292102146089007 1104 72-73 -0.004860596282100005 1104 74-75 -0.05155929390126346 1104 76-77 0.1272781465053967 1104 78-79 0.04393530709858595 1104 80-81 0.1869308966486578 1104 82-83 0.25392868505316457 1104 84-85 0.23252271315490702 1104 86-87 0.27914839876155284 1104 88-89 0.3228425566058064 1104 90-91 0.13080835242824662 1104 92-93 0.2521341507794368 1104 94-95 0.1995257134045474 1104 96-97 0.2548546893044943 1104 98-99 0.4485268045168844 1104 100-101 0.355931498457565 1104 102-103 0.4674194311867801 1104 104-105 0.4477513280444221 1104 106-107 0.4236923048601149 1104 108-109 0.5190236448628411 1104 110-111 0.32575816761854526 1104 112-113 0.22328030966566814 1104 114-115 0.38744463303504517 1104 116-117 0.5798542655530774 1104 118-119 0.5895804623716963 1104 120-121 0.525615955080859 1104 122-123 0.521260400653528 1104 124-125 0.45172531413978945 1105 1 0.019642783693861077 1105 2 -0.15602450023877878 1105 3 -0.06664543369747378 1105 4 -0.11050637626300386 1105 5 -0.2706856813573566 1105 6 0.038668423609017566 1105 7 -0.10037367820999066 1105 8 0.03149928637419919 1105 9 -0.14555002729067468 1105 10-11 0.09832207974336882 1105 12-13 0.1525780029574264 1105 14-15 -0.08591325817466355 1105 16-17 0.14588796476731858 1105 18-19 0.1762570890663966 1105 20-21 0.19590169145628522 1105 22-23 0.05347782030967352 1105 24-25 0.16965764147963114 1105 26-27 0.08468215150417535 1105 28-29 0.03105531071423684 1105 30-31 -0.3783190703036823 1105 32-33 -0.07538024023619982 1105 34-35 -0.13209638374780752 1105 36-37 0.17895589982443738 1105 38-39 0.03921633958955795 1105 40-41 0.04964862074323406 1105 42-43 0.13756385366739465 1105 44-45 0.08564559211202294 1105 46-47 -0.34745482836282804 1105 48-49 -0.368616544521295 1105 50-51 -0.49456989317005906 1105 52-53 -0.414878131170326 1105 54-55 -0.32826603188497216 1105 56-57 -0.3568400165123222 1105 58-59 -0.5736235926435285 1105 60-61 -0.3683035403446837 1105 62-63 -0.34234642327334797 1105 64-65 -0.4697640608702045 1105 66-67 -0.571415182226616 1105 68-69 -0.5447094522428202 1105 70-71 -0.2511320196047748 1105 72-73 -0.2079221649640175 1105 74-75 -0.2523569287749723 1105 76-77 -0.3550667412899955 1105 78-79 -0.1940654468846219 1105 80-81 -0.2087614782000493 1105 82-83 -0.22793008198586406 1105 84-85 -0.2889916026053285 1105 86-87 -0.1923212732557502 1105 88-89 -0.14845647710517795 1105 90-91 -0.23522470378318516 1105 92-93 -0.17339810184462578 1105 94-95 -0.02659186829350446 1105 96-97 -0.05773286203580952 1105 98-99 -0.11295457408189336 1105 100-101 -0.03397703147358655 1105 102-103 0.016128730974475758 1105 104-105 0.013768510925036281 1105 106-107 -0.023245134374786147 1105 108-109 -0.009292393153788936 1105 110-111 -0.1549319254388557 1105 112-113 -0.04672107925077995 1105 114-115 -0.13868882199382426 1105 116-117 -0.3397810446713301 1105 118-119 -0.14743614510067715 1105 120-121 -0.03630590844818471 1105 122-123 0.03788176218847994 1105 124-125 0.44488986105540107 1106 1 -0.049530327354410986 1106 2 -0.07338068500094153 1106 3 0.06430767907203006 1106 4 -0.1581066893636489 1106 5 -0.18471414728717406 1106 6 -0.08004753591739444 1106 7 -0.02633603236616011 1106 8 -0.028772586615133378 1106 9 -0.043218575020084415 1106 10-11 -0.21620882255201934 1106 12-13 -0.11968021490979197 1106 14-15 -0.11967812943046141 1106 16-17 -0.09948212100750453 1106 18-19 -0.09685764756432746 1106 20-21 0.21049734493092842 1106 22-23 -0.19494130833616197 1106 24-25 -0.21391113205926615 1106 26-27 -0.2027208754085521 1106 28-29 -0.23705145496435165 1106 30-31 -0.04590167928443378 1106 32-33 0.08439739273936908 1106 34-35 0.03321173760737395 1106 36-37 -0.19358468236242743 1106 38-39 -0.37804353803890933 1106 40-41 -0.47213728235130503 1106 42-43 -0.30560059383702054 1106 44-45 -0.4843164177973662 1106 46-47 -0.44787038339927676 1106 48-49 -0.4263803338586598 1106 50-51 -0.09120693002495983 1106 52-53 -0.05652543395586207 1106 54-55 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-0.24817743825941108 2216 24-25 -0.20207608571502078 2216 26-27 -0.17916697532090353 2216 28-29 -0.1278420294205702 2216 30-31 -0.3065814391973163 2216 32-33 -0.3154545446892598 2216 34-35 -0.28904014139296663 2216 36-37 -0.16647196890001936 2216 38-39 -0.09800941061214274 2216 40-41 -0.19817006858347241 2216 42-43 -0.4373115464835564 2216 44-45 -0.3261666687733893 2216 46-47 -0.33648483643091254 2216 48-49 -0.21523347969033324 2216 50-51 -0.21496034940721387 2216 52-53 -0.038487362620237775 2216 54-55 -0.23001596031325278 2216 56-57 -0.44029263603708557 2216 58-59 -0.268780139503491 2216 60-61 -0.12832195379495914 2216 62-63 -0.44634988474173554 2216 64-65 -0.3643958569298533 2216 66-67 -0.5727750448089282 2216 68-69 -0.4148995076783635 2216 70-71 -0.472246326139917 2216 72-73 -0.13990463640039508 2216 74-75 -0.048859023874257446 2216 76-77 -0.19899323063231833 2216 78-79 -0.2919348870636824 2216 80-81 -0.364880534494457 2216 82-83 -0.291072600789704 2216 84-85 -0.18247825122724493 2216 86-87 -0.4174875148298085 2216 88-89 -0.3438830445257324 2216 90-91 -0.4328537280655169 2216 92-93 -0.4373122938650269 2216 94-95 -0.32538588489813947 2216 96-97 -0.5618628753091102 2216 98-99 -0.5091593212385455 2216 100-101 -0.5094246800174282 2216 102-103 -0.611168070420522 2216 104-105 -0.5821798793620339 2216 106-107 -0.5391275485537967 2216 108-109 -0.5115778081396023 2216 110-111 -0.4100525563109869 2216 112-113 -0.3675998497788484 2216 114-115 -0.6661251882327974 2216 116-117 -0.5437441657186177 2216 118-119 -0.584901985873131 2216 120-121 -0.48959235146408275 2216 122-123 -0.3256391107261827 2216 124-125 -0.40728201516456863 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 10.0 5 31.0 6 94.0 7 196.0 8 406.0 9 826.0 10 1574.0 11 2321.0 12 2721.0 13 4195.0 14 7911.0 15 8343.0 16 2276.0 17 1523.0 18 1509.0 19 1689.0 20 1956.0 21 2009.0 22 1920.0 23 943.0 24 1054.0 25 1263.0 26 1611.0 27 1963.0 28 2388.0 29 2986.0 30 3861.0 31 4669.0 32 6145.0 33 8349.0 34 11537.0 35 17924.0 36 33881.0 37 115340.0 38 88551.0 39 111.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.86848493129181 23.232003162873447 15.772796381300674 21.126715524534074 2 14.266075710237594 23.666351812831504 41.849887379205114 20.217685097725788 3 14.115985328907978 33.64934695040078 32.659455123547566 19.575212597143672 4 9.704911357058712 20.911008013496417 42.03231576957198 27.35176485987289 5 9.184572057998645 40.6754883354598 36.70399358283175 13.43594602370981 6 23.71651574831761 44.03075536692781 18.448278368046974 13.804450516707606 7 21.999186259408877 36.453544130895985 26.14112586823215 15.406143741462989 8 23.47845040541719 35.00043593245953 23.418001104362233 18.10311255776105 9 26.42302885872882 15.135574994914123 22.714696736318988 35.72669941003807 10-11 20.809381266529105 29.653869627132433 29.967886308814556 19.568862797523902 12-13 24.092243308436746 27.885001017175735 30.09038332994275 17.932372344444765 14-15 21.80490569327792 28.427446307652065 29.338545148071727 20.429102850998284 16-17 17.345171321456597 31.754628149612017 30.611758551541747 20.28844197738964 18-19 17.53102386003662 27.907233572611815 36.18108634368915 18.380656223662413 20-21 18.684355837135634 27.24287250428667 36.325379987793895 17.747391670783806 22-23 18.842454009125518 27.310296724694123 36.15551163939667 17.69173762678369 24-25 18.534911803213415 27.81406527681105 36.28572300440148 17.365299915574045 26-27 17.451538841582146 28.203958266732542 36.25185271295301 18.09265017873231 28-29 18.05872617073805 25.86674649109168 37.88153338457984 18.192993953590427 30-31 18.21377553467135 26.896690029398385 36.91742729966446 17.97210713626581 32-33 17.26248946496556 27.83486878432968 36.69955534889128 18.20308640181348 34-35 18.018686971431894 26.794879246708707 36.90952948356534 18.27690429829405 36-37 18.04128879634164 26.69037180841557 35.327539281318614 19.94080011392418 38-39 17.48205411374931 27.115144293644107 36.54029469034265 18.86250690226394 40-41 17.519834926908658 26.725856607282978 36.87538144090209 18.878927024906275 42-43 18.201342671975354 26.24604028015926 35.27550931442355 20.27710773344183 44-45 17.743796464755086 26.77125535489046 34.91490301617656 20.570045164177888 46-47 19.03148227532641 26.38030123589546 33.61504871651421 20.97316777226392 48-49 18.93341858234764 27.29155162893429 32.02069226074574 21.754337527972332 50-51 19.20571374597662 27.39183190077961 31.294093712990346 22.108360640253427 52-53 18.637711063965824 27.94559562905054 31.910401348484406 21.50629195849923 54-55 19.31836015833435 28.049418452578163 30.882464063798746 21.74975732528874 56-57 19.76099241621729 28.25732839857072 29.48535192152042 22.49632726369157 58-59 18.1884365445647 27.84581671917323 31.883493565524695 22.082253170737378 60-61 19.697172251445842 29.342468540207506 29.648783748437907 21.311575459908745 62-63 19.882090860079895 29.424099442423753 30.758242668460834 19.935567029035525 64-65 20.118982919818766 28.304653237736243 31.082762008003883 20.493601834441108 66-67 19.626115260542303 29.330988985439856 30.12947194048069 20.913423813537154 68-69 19.970298123143632 28.74339554646222 30.69668629354289 20.58962003685125 70-71 20.33128634660823 27.77178762101005 30.924805933452106 20.97212009892962 72-73 19.255439110444602 28.109578530699075 30.266913119547084 22.368069239309236 74-75 19.103286930744865 28.500828271673107 30.04141358365544 22.354471213926587 76-77 18.23195107030084 28.283667867434108 31.851531087476804 21.632849974788243 78-79 18.22317934589122 27.592625691504896 31.51707492156769 22.667120041036192 80-81 18.474817634921095 29.15545351506873 29.86936557296056 22.50036327704961 82-83 19.212845569804195 27.188433174701203 30.390235041958803 23.2084862135358 84-85 18.302188380946845 26.551483623470602 30.75735999302508 24.38896800255747 86-87 18.11995396446196 27.741788294650693 30.590382525095762 23.547875215791585 88-89 18.599771273789923 26.868209236528184 31.067820082277496 23.464199407404394 90-91 18.468448831072205 26.57247236075751 30.987775956474735 23.971302851695555 92-93 19.020389420345243 27.00468356508964 30.370659927835607 23.60426708672951 94-95 18.17278224136503 26.05959942513453 31.334963765746675 24.43265456775377 96-97 18.65456490022727 26.32163263408141 30.636447125975785 24.387355339715533 98-99 18.11166993386645 26.623149212457115 30.877726319582997 24.387454534093436 100-101 18.07057161789837 26.20903521315058 31.196160150664387 24.524233018286658 102-103 19.13570649070214 25.78276557066651 30.394568775983803 24.686959162647547 104-105 17.806029553443373 27.040281787970244 30.5465018447289 24.60718681385748 106-107 18.355517451829463 26.203754831584757 30.563079426894124 24.87764828969165 108-109 18.581619648413035 26.056439342747158 29.80394892296156 25.55799208587825 110-111 18.21678560478252 26.98754528294484 29.4590168460547 25.33665226621794 112-113 17.60267731936741 26.92257949200103 30.582677348430792 24.892065840200768 114-115 17.731116858961318 26.986398907262632 29.916446278589902 25.366037955186144 116-117 17.61879159522218 27.09625388706443 29.77970879711703 25.505245720596353 118-119 17.41548298034657 26.342863370508958 29.91782613434083 26.32382751480365 120-121 17.212066610479816 26.750850068296085 29.64805719433869 26.389026126885405 122-123 17.228661978891935 26.97868123277141 29.71694617487173 26.07571061346493 124-125 17.1196801744503 27.184008722514992 29.216209340359807 26.480101762674906 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 446.0 1 315.5 2 266.0 3 309.5 4 164.0 5 100.0 6 250.5 7 547.5 8 908.5 9 995.0 10 677.5 11 379.0 12 323.5 13 347.0 14 393.5 15 454.5 16 500.5 17 540.0 18 567.0 19 594.5 20 625.5 21 686.0 22 787.5 23 875.0 24 952.0 25 1109.0 26 1295.5 27 1575.0 28 1882.0 29 2523.5 30 3721.5 31 4816.5 32 7220.0 33 10379.0 34 12170.0 35 14520.5 36 20218.5 37 24387.0 38 23828.0 39 22776.0 40 21180.5 41 18144.0 42 14435.0 43 13909.5 44 15104.5 45 14802.0 46 12595.0 47 10374.5 48 9291.0 49 7611.5 50 6320.5 51 4773.0 52 3675.0 53 3224.5 54 2903.5 55 2750.5 56 2722.0 57 2455.5 58 2073.5 59 1916.0 60 1785.0 61 1698.0 62 1459.0 63 839.0 64 378.5 65 462.5 66 501.0 67 318.5 68 178.0 69 115.5 70 113.5 71 116.0 72 114.0 73 161.5 74 150.0 75 85.0 76 51.5 77 39.5 78 32.0 79 14.5 80 3.5 81 1.5 82 3.0 83 1.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02877154232904182 2 0.004359324595309366 3 0.003487459676247493 4 0.08573338370775088 5 0.003778081315934785 6 0.024702839373419747 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 4.359324595309366E-4 26-27 0.0 28-29 4.359324595309366E-4 30-31 0.0068296085326513415 32-33 0.0 34-35 0.0 36-37 4.359324595309366E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.003487459676247493 46-47 0.002179662297654683 48-49 0.0 50-51 0.002179662297654683 52-53 0.0 54-55 0.002324973117498329 56-57 1.4531081984364556E-4 58-59 0.0017437298381237465 60-61 0.0 62-63 0.003342148856403848 64-65 0.002034351477811038 66-67 0.0 68-69 0.0011624865587491645 70-71 0.002034351477811038 72-73 0.002324973117498329 74-75 0.0 76-77 0.0013077973785928102 78-79 0.001017175738905519 80-81 0.0 82-83 7.265540992182277E-4 84-85 0.0 86-87 0.002324973117498329 88-89 0.0030515272167165567 90-91 0.004068702955622076 92-93 0.004214013775465721 94-95 0.0027609055770292656 96-97 0.002324973117498329 98-99 0.00392339213577843 100-101 0.004068702955622076 102-103 0.001598419018280101 104-105 4.359324595309366E-4 106-107 0.0 108-109 0.006248365253276759 110-111 0.001017175738905519 112-113 0.004214013775465721 114-115 0.0 116-117 0.0 118-119 7.265540992182277E-4 120-121 0.0 122-123 0.001017175738905519 124-125 0.04431980005231189 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 344090.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.358423668226337 #Duplication Level Percentage of deduplicated Percentage of total 1 70.5563988354443 16.48086256502659 2 10.8592330853261 5.073091342381353 3 4.683106477219001 3.2816995553488915 4 2.670017667404882 2.4946961550757067 5 1.7679846716599896 2.0648667499782034 6 1.2441834424067484 1.7437298381237465 7 0.9281608480354343 1.5176262024470342 8 0.756463532983303 1.413583655438984 9 0.5723243835071044 1.2031735883053851 >10 4.638315873292358 22.225871138364965 >50 0.7353124144623883 12.052951262751025 >100 0.5648592828526638 24.84291900374902 >500 0.01741856819369448 2.689703275305879 >1k 0.0062209172120337415 2.915225667703217 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2959 0.8599494318346943 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2496 0.7253916126594786 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT 2088 0.6068179836670639 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1429 0.41529832311313897 No Hit ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT 1059 0.3077683164288413 RNA PCR Primer, Index 31 (95% over 22bp) ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 881 0.2560376645645035 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 829 0.24092533930076435 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 791 0.22988171699264728 No Hit ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT 760 0.22087244616234128 RNA PCR Primer, Index 31 (95% over 24bp) GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 726 0.21099131041297337 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 703 0.20430701270016566 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 702 0.20401639106047836 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 649 0.18861344415705195 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 636 0.18483536284111715 No Hit ACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTT 543 0.15780755035019908 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 521 0.15141387427707867 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 508 0.14763579296114387 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 504 0.14647330640239473 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 502 0.14589206312302014 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 490 0.14240460344677264 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 490 0.14240460344677264 No Hit CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC 487 0.14153273852771078 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 482 0.1400796303292743 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 467 0.13572030573396496 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 460 0.13368595425615393 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 460 0.13368595425615393 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 459 0.1333953326164666 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 456 0.13252346769740475 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 448 0.13019849457990643 No Hit ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC 446 0.12961725130053184 No Hit TATATAATCCCAACAAACGACCTAAAACCTGGTGAACTACGACTGCTAGA 446 0.12961725130053184 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 444 0.12903600802115725 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 442 0.12845476474178266 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 437 0.12700165654334622 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 431 0.12525792670522248 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 429 0.1246766834258479 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 414 0.12031735883053851 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 414 0.12031735883053851 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 409 0.11886425063210207 No Hit CCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATGACGAGAC 408 0.11857362899241478 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 406 0.11799238571304019 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 400 0.11624865587491645 No Hit TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA 398 0.11566741259554188 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 389 0.11305181783835626 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 387 0.11247057455898166 No Hit GCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGTACTGAAG 376 0.10927373652242146 No Hit CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG 373 0.10840187160335958 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 373 0.10840187160335958 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 366 0.10636752012554854 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 362 0.10520503356679937 No Hit TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG 359 0.10433316864773752 RNA PCR Primer, Index 31 (96% over 29bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 357 0.10375192536836292 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 355 0.10317068208898834 No Hit CTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAGGTCAGCAGCCTCCT 353 0.10258943880961376 No Hit GTCCTAGGGAGGGGACTGCTCATGAGTGGAGGACGTCTTCAGATGAAATT 351 0.10200819553023918 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 349 0.10142695225086461 No Hit CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT 346 0.10055508733180273 RNA PCR Primer, Index 31 (96% over 27bp) GCCTGGGATGGCATCAGTTTTAAGTCCTAGGGAGGGGACTGCTCATGAGT 345 0.10026446569211545 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.812432793745822E-4 0.0 18-19 0.0 0.0 0.0 0.0011624865587491645 0.0 20-21 0.0 0.0 0.0 0.0014531081984364555 0.0 22-23 0.0 0.0 0.0 0.004359324595309366 0.0 24-25 0.0 0.0 0.0 0.011043622308117063 0.0 26-27 0.0 0.0 0.0 0.024702839373419744 0.0 28-29 0.0 0.0 0.0 0.045772908250748354 0.0 30-31 0.0 0.0 0.0 0.1046237902874248 0.0 32-33 0.0 0.0 0.0 0.19893051236595077 0.0 34-35 0.0 0.0 0.0 0.3218634659536749 0.0 36-37 0.0 0.0 0.0 0.521084599959313 0.0 38-39 0.0 0.0 0.0 0.8162108750617572 0.0 40-41 0.0 0.0 0.0 1.1563835043157313 0.0 42-43 0.0 0.0 0.0 1.4552878607341104 0.0 44-45 0.0 0.0 0.0 1.776424772588567 0.0 46-47 0.0 0.0 0.0 2.1411549303961173 0.0 48-49 0.0 0.0 0.0 2.5333488331541165 0.0 50-51 0.0 0.0 0.0 2.9659391438286495 0.0 52-53 0.0 0.0 0.0 3.367142317416955 0.0 54-55 0.0 0.0 0.0 3.7501816385248046 0.0 56-57 0.0 0.0 0.0 4.175942340666686 0.0 58-59 0.0 0.0 0.0 4.682641169461478 0.0 60-61 0.0 0.0 0.0 5.218402162224999 0.0 62-63 0.0 0.0 0.0 5.741811735301811 0.0 64-65 0.0 0.0 0.0 6.24720276671801 0.0 66-67 0.0 0.0 0.0 6.807375977215264 0.0 68-69 0.0 0.0 0.0 7.39036298642797 0.0 70-71 0.0 0.0 0.0 8.06445987968264 0.0 72-73 0.0 0.0 0.0 8.73129123194513 0.0 74-75 0.0 0.0 0.0 9.395071056990904 0.0 76-77 0.0 0.0 0.0 10.077450666976663 0.0 78-79 0.0 0.0 0.0 10.856026039698916 0.0 80-81 0.0 0.0 0.0 11.65697927867709 0.0 82-83 0.0 0.0 0.0 12.519544305268969 0.0 84-85 0.0 0.0 0.0 13.386178034816473 0.0 86-87 0.0 0.0 0.0 14.240896277136795 0.0 88-89 0.0 0.0 0.0 15.111889331279606 0.0 90-91 0.0 0.0 0.0 16.126158853788255 0.0 92-93 0.0 0.0 0.0 17.22223836786887 0.0 94-95 0.0 0.0 0.0 18.30959923275887 0.0 96-97 0.0 0.0 0.0 19.403935017001366 0.0 98-99 0.0 0.0 0.0 20.500014531081984 0.0 100-101 0.0 0.0 0.0 21.699410038071434 0.0 102-103 0.0 0.0 0.0 22.983376442209888 0.0 104-105 0.0 0.0 0.0 24.26138510273475 2.906216396872911E-4 106-107 0.0 0.0 0.0 25.562207561975065 2.906216396872911E-4 108-109 0.0 0.0 0.0 26.804469760818392 2.906216396872911E-4 110-111 0.0 0.0 0.0 28.119387369583542 2.906216396872911E-4 112-113 0.0 0.0 0.0 29.452904763288675 2.906216396872911E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTGGCG 15 2.6947417E-4 119.05919 3 GGCGAGC 15 2.699425E-4 119.00727 6 GCGAGCA 15 2.7025506E-4 118.97268 7 GGTATCA 1185 0.0 92.47448 1 TTGGCGA 20 8.45977E-4 89.29439 4 AGATAGT 75 0.0 79.33818 6 GATAGTC 85 0.0 69.983925 7 GTATCAA 2660 0.0 66.04848 1 TAGTCAA 100 0.0 65.434975 9 CGGAGAT 30 0.0042184032 59.555584 1 TAGATAG 115 0.0 56.93307 5 GTACAGG 45 3.153228E-4 52.938293 1 ATACTCG 70 9.118485E-7 51.003113 6 TACTCGC 70 9.136802E-7 50.98829 7 GTTCGAC 60 7.2759576E-12 49.57195 16-17 ATCAACG 3755 0.0 46.609062 3 TCAACGC 3770 0.0 46.42361 4 CAACGCA 3810 0.0 45.929543 5 TCTAGAT 130 1.8189894E-11 45.791996 3 CGACCGT 65 1.8189894E-11 45.758724 18-19 >>END_MODULE