FastQCFastQC Report
Fri 27 May 2016
SRR940095_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940095_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224890
Sequences flagged as poor quality0
Sequence length125
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23611.0498465916670372No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7360.3272711103206012No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6900.3068166659255636No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6500.2890301925385744No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5830.2592378496153675No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT4630.2058784294543999No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.18542398505936233No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3700.16452487882965006No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT3390.15074036195473342No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT2810.1249499755435991No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT2770.12317132820490018No Hit
GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG2600.11561207701542976No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT2560.11383342967673084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2470.10983147316465827No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATAGT250.002051473471.428186
GGTATCA6100.071.264751
GTATCAA13400.065.7716451
GTACAAG1750.057.8485681
CGTCACT551.3008123E-554.1363641
ACAGTAC453.1592694E-452.9097638
CGCAGAA602.1768574E-549.6252
ATCAACG16750.044.44033
TCAACGC16950.043.9159284
CAACGCA17950.041.469365
TATCAAC20950.041.215992
TATAGGG600.001300882439.72
GTACATG37950.038.9154131
TACATGG38400.037.5289042
AACGCAG20150.036.925246
GTACATA1209.183732E-734.73751
GAACAAA4150.034.4385571
GAGAACA8400.034.028571
GTCACTA1051.4699142E-534.028572
TACAAGG1953.8198777E-1133.5923042
Produced by FastQC (version 0.11.5)