##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940095_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 224890 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.885926452932544 31.0 28.0 34.0 16.0 34.0 2 28.731317533016142 31.0 27.0 34.0 15.0 34.0 3 29.003632887189294 33.0 27.0 34.0 18.0 34.0 4 31.28506825559162 37.0 30.0 37.0 12.0 37.0 5 32.104433278491705 37.0 31.0 37.0 17.0 37.0 6 31.291889368135532 37.0 31.0 37.0 10.0 37.0 7 31.260469562897416 37.0 31.0 37.0 8.0 37.0 8 30.761181021832897 37.0 31.0 37.0 8.0 37.0 9 31.487963004135356 39.0 29.0 39.0 8.0 39.0 10-11 32.012001422917876 39.0 31.0 39.0 8.0 39.0 12-13 31.846747298679354 39.0 30.5 39.0 8.0 39.0 14-15 33.00765040686558 40.0 31.0 41.0 8.0 41.0 16-17 33.37658855440438 40.0 31.0 41.0 9.0 41.0 18-19 33.561561207701544 40.0 31.0 41.0 12.0 41.0 20-21 33.836517853172666 40.0 31.0 41.0 13.0 41.0 22-23 34.18585753034817 40.0 31.0 41.0 17.5 41.0 24-25 34.50036462270443 40.0 31.0 41.0 22.0 41.0 26-27 34.65833518609098 40.0 31.0 41.0 24.5 41.0 28-29 34.6319222731113 40.0 31.0 41.0 25.0 41.0 30-31 34.478816310196095 40.0 31.0 41.0 25.0 41.0 32-33 34.326017163946815 40.0 31.0 41.0 25.0 41.0 34-35 34.121526079416604 40.0 30.5 41.0 23.5 41.0 36-37 33.8164080216995 39.5 30.0 41.0 19.5 41.0 38-39 33.53095513362088 39.0 30.0 41.0 12.0 41.0 40-41 33.264049090666546 39.0 30.0 41.0 2.0 41.0 42-43 33.03681132998355 39.0 30.0 41.0 2.0 41.0 44-45 32.946822891191246 39.0 30.0 41.0 2.0 41.0 46-47 32.739092445195425 39.0 30.0 41.0 2.0 41.0 48-49 32.51431588776735 39.0 30.0 41.0 2.0 41.0 50-51 32.325250122282 38.5 30.0 41.0 2.0 41.0 52-53 32.07519676286184 38.0 30.0 41.0 2.0 41.0 54-55 31.820854640046242 38.0 30.0 41.0 2.0 41.0 56-57 31.545228778513938 37.0 30.0 41.0 2.0 41.0 58-59 31.22000088932367 37.0 30.0 41.0 2.0 41.0 60-61 30.891740406420915 36.0 30.0 40.0 2.0 41.0 62-63 30.52814709413491 36.0 30.0 40.0 2.0 41.0 64-65 30.174658722041887 35.0 29.5 40.0 2.0 41.0 66-67 29.819742985459555 35.0 29.0 39.5 2.0 41.0 68-69 29.440361954733426 35.0 28.5 39.0 2.0 41.0 70-71 29.303299390813287 35.0 29.0 39.0 2.0 41.0 72-73 29.034332340255233 35.0 28.5 38.5 2.0 41.0 74-75 28.650148961714613 35.0 28.0 37.0 2.0 40.0 76-77 28.27974120681222 35.0 28.0 37.0 2.0 39.0 78-79 27.925843745831294 35.0 27.5 36.0 2.0 39.0 80-81 27.574972208635334 35.0 27.0 36.0 2.0 38.5 82-83 27.25790164080217 35.0 26.0 35.5 2.0 37.0 84-85 26.950735915336388 35.0 25.0 35.0 2.0 37.0 86-87 26.692885410645204 35.0 25.0 35.0 2.0 36.5 88-89 26.454021966294633 35.0 24.5 35.0 2.0 36.0 90-91 26.266445817955443 35.0 23.5 35.0 2.0 36.0 92-93 26.080735026012718 35.0 21.5 35.0 2.0 36.0 94-95 25.91998310285028 35.0 20.0 35.0 2.0 35.0 96-97 25.746444928631774 34.0 18.0 35.0 2.0 35.0 98-99 25.629994664057982 34.0 16.0 35.0 2.0 35.0 100-101 25.532137934101115 34.0 12.5 35.0 2.0 35.0 102-103 24.827626839788344 32.5 8.0 35.0 2.0 35.0 104-105 25.263399884387923 34.0 7.0 35.0 2.0 35.0 106-107 25.29089999555338 34.0 5.0 35.0 2.0 35.0 108-109 25.204993552403394 34.0 2.0 35.0 2.0 35.0 110-111 25.06838009693628 34.0 2.0 35.0 2.0 35.0 112-113 24.935408421895147 34.0 2.0 35.0 2.0 35.0 114-115 24.75890435323936 34.0 2.0 35.0 2.0 35.0 116-117 24.625012228200454 34.0 2.0 35.0 2.0 35.0 118-119 24.41119658499711 34.0 2.0 35.0 2.0 35.0 120-121 24.142785361732404 34.0 2.0 35.0 2.0 35.0 122-123 23.850755925118946 34.0 2.0 35.0 2.0 35.0 124-125 22.010073813864558 30.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6535064551833685 1101 2 0.8119885614238349 1101 3 0.43201720803484633 1101 4 0.3027129893027585 1101 5 0.5727499164707766 1101 6 0.637773515065831 1101 7 0.683494004738872 1101 8 0.30372029402014533 1101 9 0.6601334303497666 1101 10-11 0.9601660698450836 1101 12-13 0.8520492024313135 1101 14-15 1.10411871580515 1101 16-17 0.6267416321782306 1101 18-19 0.6519966868865197 1101 20-21 1.0455112977343006 1101 22-23 0.7865297954691144 1101 24-25 0.6313711791066865 1101 26-27 0.9833893516717893 1101 28-29 0.9297412941785126 1101 30-31 0.9759345397221466 1101 32-33 0.7145340756904943 1101 34-35 0.8261678810744968 1101 36-37 0.8334082742406324 1101 38-39 1.100247251564106 1101 40-41 1.106871993728248 1101 42-43 1.2307084234570596 1101 44-45 1.1042209398307037 1101 46-47 1.2314785952229244 1101 48-49 1.4424330578236848 1101 50-51 1.2674313535406085 1101 52-53 1.1715306440649655 1101 54-55 1.1411722780305062 1101 56-57 1.103187531863437 1101 58-59 0.9580415195973337 1101 60-61 0.6685491769004948 1101 62-63 0.9841191493920078 1101 64-65 0.8433015515034761 1101 66-67 0.8898010244873049 1101 68-69 1.1258621976521788 1101 70-71 0.8608412955781368 1101 72-73 0.8886128934674566 1101 74-75 1.0764662259466071 1101 76-77 0.9530274075631162 1101 78-79 1.0835318063981951 1101 80-81 1.1289051604894382 1101 82-83 1.065938753202058 1101 84-85 0.9621690522280382 1101 86-87 1.0355292234229054 1101 88-89 1.2175919879632104 1101 90-91 1.1390804608584304 1101 92-93 1.1125016841256326 1101 94-95 1.2205026950140265 1101 96-97 1.1808012941156818 1101 98-99 1.1879511098212134 1101 100-101 1.3356277044687666 1101 102-103 1.1828076730273516 1101 104-105 1.2021188811598371 1101 106-107 1.187140449934443 1101 108-109 1.0799127481022985 1101 110-111 1.1125454870228388 1101 112-113 0.9351524273207161 1101 114-115 1.0492772594643043 1101 116-117 0.813511665545466 1101 118-119 1.022993603700364 1101 120-121 0.5531806337774512 1101 122-123 0.7190445811931383 1101 124-125 0.6176568350899885 1102 1 -0.14197317130219744 1102 2 -0.07122451158125642 1102 3 -0.3986491756663426 1102 4 -0.5481146847210105 1102 5 -0.09739015144093344 1102 6 0.017633447154114634 1102 7 -0.11003315994703655 1102 8 -0.21620754978291146 1102 9 -0.10453125555855536 1102 10-11 0.04065206305390845 1102 12-13 -0.4719427330525612 1102 14-15 -0.2754865473527346 1102 16-17 -0.452715074103601 1102 18-19 -0.4616917681219661 1102 20-21 -0.903650417036495 1102 22-23 -0.5654544999468456 1102 24-25 -0.7090150687710732 1102 26-27 -0.9373979997713349 1102 28-29 -0.7932744104055303 1102 30-31 -0.7613001801420296 1102 32-33 -1.0136386747339614 1102 34-35 -0.8776309304704881 1102 36-37 -0.7017466493586895 1102 38-39 -0.7248906941065272 1102 40-41 -0.5937752898031547 1102 42-43 -0.6181667887670486 1102 44-45 -0.7520184489638595 1102 46-47 -0.9538142740470263 1102 48-49 -0.795459556777331 1102 50-51 -1.0203233153897884 1102 52-53 -0.6778220724036288 1102 54-55 -0.6386664316469144 1102 56-57 -0.4342806345202632 1102 58-59 -0.7332042359204927 1102 60-61 -1.2207759504339712 1102 62-63 -1.194308269962832 1102 64-65 -0.9385851208224985 1102 66-67 -0.8730173116756852 1102 68-69 -1.165914118137291 1102 70-71 -1.200374748564478 1102 72-73 -1.3218603662948496 1102 74-75 -1.3830838589430385 1102 76-77 -1.4805252240158282 1102 78-79 -1.4610670900364404 1102 80-81 -1.265651291123465 1102 82-83 -1.3574165099558329 1102 84-85 -1.4737923229841847 1102 86-87 -1.4583056662205571 1102 88-89 -1.0864402701013063 1102 90-91 -1.1615668226729774 1102 92-93 -1.067315803140918 1102 94-95 -0.9621373728976863 1102 96-97 -1.1559028654768468 1102 98-99 -1.1518302993468694 1102 100-101 -1.1791218710830158 1102 102-103 -1.171691478076216 1102 104-105 -1.0934880797909265 1102 106-107 -1.2417856960757412 1102 108-109 -1.1430074556328442 1102 110-111 -1.334133205676654 1102 112-113 -1.1667045336300461 1102 114-115 -1.1934965096358674 1102 116-117 -1.2438312886141212 1102 118-119 -1.3615246475729776 1102 120-121 -1.8035680928778959 1102 122-123 -1.4295016836625507 1102 124-125 -1.2719654059965997 1103 1 -0.037338100615773584 1103 2 0.0501240661738116 1103 3 0.3751010372445762 1103 4 -0.06117676954471918 1103 5 0.011950250534873419 1103 6 1.0679123604567877 1103 7 0.6671875009284527 1103 8 0.7761808848960179 1103 9 0.8804904613550875 1103 10-11 0.9922414430573134 1103 12-13 0.9173169756853063 1103 14-15 1.0591348970349301 1103 16-17 0.9535189575775505 1103 18-19 0.602341190488147 1103 20-21 0.5967274998430767 1103 22-23 0.4254169444408191 1103 24-25 0.3824621071785046 1103 26-27 0.6228611128159613 1103 28-29 0.39748844941392747 1103 30-31 0.6115228057206892 1103 32-33 0.5058692881343845 1103 34-35 0.5378966627844903 1103 36-37 0.1331994841790376 1103 38-39 -0.3346728862373354 1103 40-41 -0.04253295459620432 1103 42-43 0.4434602764688549 1103 44-45 0.15449236691078028 1103 46-47 0.06787070295859365 1103 48-49 0.21410546621704896 1103 50-51 0.29845755669332874 1103 52-53 0.4748190875350602 1103 54-55 0.3754921444048698 1103 56-57 0.42228660274766483 1103 58-59 0.20607889301836124 1103 60-61 0.07963070941954697 1103 62-63 -0.06293691073953056 1103 64-65 0.1360774153723554 1103 66-67 0.4470303803699629 1103 68-69 0.5030466636716007 1103 70-71 0.576531868706855 1103 72-73 0.03398297385163218 1103 74-75 0.25354107718368724 1103 76-77 0.34040604833981547 1103 78-79 0.41359757526759466 1103 80-81 0.3738106829865693 1103 82-83 0.2287219247230965 1103 84-85 0.3405227425923769 1103 86-87 0.31458758024512434 1103 88-89 0.2877506684100197 1103 90-91 0.26993441221034686 1103 92-93 0.1747106829772882 1103 94-95 0.24530173457974058 1103 96-97 0.2016437620142142 1103 98-99 0.2894648377677669 1103 100-101 -0.20832260349657616 1103 102-103 -0.04684051571886627 1103 104-105 0.20542820363608527 1103 106-107 0.2503046633178876 1103 108-109 0.14233575676708554 1103 110-111 -0.1489379663647803 1103 112-113 0.09833229995877701 1103 114-115 0.08929709452015544 1103 116-117 0.27309512937258873 1103 118-119 0.2803325743653602 1103 120-121 0.24819055130537748 1103 122-123 0.3764566283796924 1103 124-125 0.8124005452893819 1104 1 0.4019132215090977 1104 2 0.33787369124287636 1104 3 0.5739920310088813 1104 4 0.936721643905436 1104 5 0.3385052272496907 1104 6 0.7858458990154773 1104 7 0.5179353894753014 1104 8 1.1426660060579081 1104 9 1.1460303618997258 1104 10-11 0.7500952594595596 1104 12-13 0.8169292181669512 1104 14-15 1.0971065206832051 1104 16-17 1.2150480837911388 1104 18-19 0.7790816593491456 1104 20-21 1.0764121631945684 1104 22-23 0.8277178363817725 1104 24-25 0.5915305024741073 1104 26-27 0.3698468654404792 1104 28-29 0.3105044727780495 1104 30-31 0.39318473641766616 1104 32-33 0.5105509915048074 1104 34-35 0.32575482049227844 1104 36-37 0.5820707447362992 1104 38-39 0.3305088565995149 1104 40-41 0.6342815137911941 1104 42-43 0.7236175501289708 1104 44-45 0.7608987525765727 1104 46-47 0.5225977927052199 1104 48-49 0.6999271569582248 1104 50-51 0.7748467744690046 1104 52-53 0.6993040508312944 1104 54-55 0.5838945439628382 1104 56-57 0.7241356042473832 1104 58-59 0.768761425183488 1104 60-61 0.7891727016841301 1104 62-63 1.045783193451804 1104 64-65 1.1606205916293604 1104 66-67 1.0001373738185428 1104 68-69 1.1962890269204713 1104 70-71 1.5462956622185793 1104 72-73 1.560721469391929 1104 74-75 1.5386908522251268 1104 76-77 1.5304664319533607 1104 78-79 1.4377214208749827 1104 80-81 1.3707816357058036 1104 82-83 1.424475338567916 1104 84-85 1.1789275101351997 1104 86-87 1.3644237946266813 1104 88-89 1.4730109494108916 1104 90-91 1.6853924199457637 1104 92-93 1.743579575549706 1104 94-95 1.6780262984758636 1104 96-97 1.716088469568085 1104 98-99 1.6631969792153924 1104 100-101 1.6967606706371399 1104 102-103 1.2904394590226325 1104 104-105 1.4234406750229347 1104 106-107 1.3950082705481357 1104 108-109 1.5777884365366042 1104 110-111 1.6136764862360558 1104 112-113 1.6421449528911332 1104 114-115 1.7517457881818501 1104 116-117 1.94856280637946 1104 118-119 1.6622638633384454 1104 120-121 1.7432868493557372 1104 122-123 1.711343951767489 1104 124-125 1.3351430664039157 1105 1 -0.33515313207369246 1105 2 -0.07001905228083061 1105 3 -0.16722142149831498 1105 4 -0.3236745081927346 1105 5 -0.6308365391493389 1105 6 -0.28755207098906865 1105 7 -0.3305913488976735 1105 8 -0.30150911892315335 1105 9 -0.004723311786079876 1105 10-11 0.2367639460221973 1105 12-13 0.1548041613573794 1105 14-15 0.02430505120887716 1105 16-17 0.17739280428803283 1105 18-19 0.1057935211882608 1105 20-21 0.05045018773630261 1105 22-23 0.02412747128557413 1105 24-25 0.11725577137276133 1105 26-27 0.14282633824763025 1105 28-29 -0.042017872321181926 1105 30-31 0.04344606045948041 1105 32-33 -0.2723322837565121 1105 34-35 -0.1585846735237766 1105 36-37 -0.2337526153606504 1105 38-39 -0.17402644108265974 1105 40-41 -0.5158188497900795 1105 42-43 -0.09967626898934867 1105 44-45 -0.2262338788429048 1105 46-47 -0.18728517957170965 1105 48-49 -0.3350864773396083 1105 50-51 -0.39419958214362794 1105 52-53 -0.0880766490626641 1105 54-55 -0.39875959934877514 1105 56-57 -0.36022661359839603 1105 58-59 -0.4727739482447788 1105 60-61 -0.4193089677598927 1105 62-63 -0.2907756612671797 1105 64-65 -0.4287882100673528 1105 66-67 -0.5564923035030773 1105 68-69 -0.5157527091801164 1105 70-71 -0.23164518007804347 1105 72-73 -0.14720157257141153 1105 74-75 0.04822539244630164 1105 76-77 0.1350149852028686 1105 78-79 0.11650115121887339 1105 80-81 0.2791856653982734 1105 82-83 0.08426173456852126 1105 84-85 0.14249363647969204 1105 86-87 0.17761497778074897 1105 88-89 0.24197587896701833 1105 90-91 0.2797436546071772 1105 92-93 0.3371260079084415 1105 94-95 0.22219574249950114 1105 96-97 0.4485532476320131 1105 98-99 0.5981594848963496 1105 100-101 0.5684467788025671 1105 102-103 0.4158371260427778 1105 104-105 0.2773643229550693 1105 106-107 0.4566655951959149 1105 108-109 0.2958614557761088 1105 110-111 0.31465929187156405 1105 112-113 0.5249941424078735 1105 114-115 0.47196810298263614 1105 116-117 0.5786188584927743 1105 118-119 0.5487299290860612 1105 120-121 0.6833148754123926 1105 122-123 0.7107095781877284 1105 124-125 0.7508565946572112 1106 1 -0.1377209248323652 1106 2 -0.4243214307083427 1106 3 -0.2888128470398996 1106 4 -0.4230344536555428 1106 5 0.012285715054574098 1106 6 0.07243126486913454 1106 7 -0.32657680564664915 1106 8 -0.30245594516160423 1106 9 -0.5978720771246664 1106 10-11 -0.3611852283453203 1106 12-13 -0.5077659037842679 1106 14-15 -0.39984469882899276 1106 16-17 -0.5501958186478859 1106 18-19 -0.3276256577240275 1106 20-21 -0.28944149534202523 1106 22-23 -0.1478919197157822 1106 24-25 -0.05023779020881847 1106 26-27 -0.10728728090099082 1106 28-29 -0.13004430770976683 1106 30-31 -0.08699819041159884 1106 32-33 -0.25011974020250705 1106 34-35 -0.14070859414186998 1106 36-37 -0.215185352824669 1106 38-39 -0.10089358242488089 1106 40-41 0.12056200159607044 1106 42-43 -0.13004098645640028 1106 44-45 -0.0912353937648902 1106 46-47 -0.15118269509965643 1106 48-49 -0.09580455035884938 1106 50-51 -0.08186054260416853 1106 52-53 -0.14215936380285754 1106 54-55 -0.4069591145737448 1106 56-57 -0.20726683477700547 1106 58-59 -0.32879955042626463 1106 60-61 -0.6132663227061137 1106 62-63 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-0.627830963372265 2216 80-81 -0.4547027095631826 2216 82-83 -0.5402078189019583 2216 84-85 -0.5058588590050874 2216 86-87 -0.7135975972893611 2216 88-89 -0.6588397972880671 2216 90-91 -0.723868521067903 2216 92-93 -0.7519229326668615 2216 94-95 -0.7627315000595658 2216 96-97 -0.7536111210448411 2216 98-99 -0.762533142447527 2216 100-101 -0.8555947465340523 2216 102-103 -0.6301336828790021 2216 104-105 -0.698712355018003 2216 106-107 -0.8187811545801615 2216 108-109 -0.9369293170239139 2216 110-111 -0.8928499221131325 2216 112-113 -0.9519672249327726 2216 114-115 -0.8274900706661015 2216 116-117 -0.8904500518342324 2216 118-119 -1.2335190173627026 2216 120-121 -1.0618578776234493 2216 122-123 -1.3633583777026175 2216 124-125 -2.0735160467829594 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9098.0 3 1695.0 4 1579.0 5 1393.0 6 2841.0 7 3231.0 8 3133.0 9 2859.0 10 2994.0 11 3189.0 12 2966.0 13 2687.0 14 2491.0 15 2368.0 16 2136.0 17 1903.0 18 1505.0 19 1370.0 20 1202.0 21 1070.0 22 944.0 23 1413.0 24 2744.0 25 2979.0 26 4398.0 27 2897.0 28 2529.0 29 3427.0 30 3259.0 31 3283.0 32 4432.0 33 6010.0 34 8822.0 35 14660.0 36 27840.0 37 53110.0 38 30299.0 39 134.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.6079502243728 20.750814991394225 12.503947093382669 19.137287690850304 2 15.137810507217376 25.33285306438272 38.03685619502477 21.492480233375137 3 18.264813785436353 35.83413007226237 27.001222901612003 18.899833240689272 4 11.049038140776158 21.136884243300344 35.265651061937064 32.54842655398643 5 11.767400692958773 46.65774152370871 27.443035497458112 14.131822285874405 6 25.21637801439259 38.751011839635645 19.938355616043552 16.094254529928214 7 26.760945456162588 40.504747292820134 18.053498765926225 14.680808485091054 8 26.854136929774242 36.57715584132617 18.945346370876297 17.62336085802329 9 30.428575875763247 20.96539640044294 16.596030436580822 32.00999728721299 10-11 23.16399128462804 34.735648539285876 22.681310863088623 19.419049312997466 12-13 25.035907560820515 33.166579954909885 23.045761573796153 18.751750910473447 14-15 23.628233180346534 32.224401394670366 22.755230013875547 21.392135411107553 16-17 21.16582217409961 35.39865311031911 23.130351262959202 20.30517345262208 18-19 21.34976364016383 32.734836727161145 26.705770915510808 19.209628717164215 20-21 25.61842719612246 30.28992596215844 25.235564844253727 18.85608199746537 22-23 24.551613125557218 31.754261537332777 24.49425162135182 19.199873715758184 24-25 22.187907167029085 31.9056639852008 26.097822365115153 19.808606482654962 26-27 21.98963937925208 31.946284850371292 25.637867401840904 20.42620836853573 28-29 21.7470284019406 31.90538022669969 25.926831762859138 20.42075960850057 30-31 21.99779001594123 32.12214052741692 25.880603054402513 19.99946640223933 32-33 20.938913901395143 32.43866377633261 25.79511978211328 20.827302540158968 34-35 21.286231682670824 32.30742208951508 26.453658352212976 19.95268787560113 36-37 21.34287340578808 32.340267525213896 25.47959764492431 20.83726142407371 38-39 21.21873728536586 32.89096596757949 25.38257847777899 20.507718269275664 40-41 20.660633282931602 33.4454771858045 25.775593702748377 20.11829582851552 42-43 19.94561459289877 33.37943271084351 26.06097124421627 20.613981452041454 44-45 20.08181777758015 33.61976966516964 25.786829116456932 20.511583440793277 46-47 20.351762704703884 33.78189378189378 25.226633461927577 20.639710051474758 48-49 19.709272335959874 33.81387737678092 25.068257412710555 21.408592874548656 50-51 20.223462444968206 33.83243651887757 25.135633921821498 20.808467114332725 52-53 20.205130713577404 34.36017271356851 24.86270516406455 20.57199140878954 54-55 20.23265327902385 34.11025239679123 24.72319062272105 20.933903701463866 56-57 20.367282138854794 34.00550545208745 24.14594339790455 21.48126901115321 58-59 20.066888888394733 33.53005663763256 24.761119054660764 21.64193541931194 60-61 19.957400733258776 33.84774097155676 24.35295137769497 21.84190691748949 62-63 20.363802921184927 33.564276963372905 24.172594046901715 21.899326068540454 64-65 20.03557334222667 33.66105274859652 24.106720026680005 22.196653882496804 66-67 20.139091149614142 33.98372971494763 23.653944065614738 22.22323506982349 68-69 20.814444427152 33.2727850788943 23.726096594105105 22.186673899848593 70-71 20.221054176283033 33.44695099987993 24.345753456333906 21.986241367503123 72-73 20.71639627461487 33.393511035419614 23.96688151292079 21.923211177044728 74-75 20.52090466830904 33.587322903974595 24.105070304787485 21.786702122928876 76-77 20.306989651384633 33.856693690768964 24.37127825634503 21.46503840150137 78-79 20.860735740743213 33.16169310193028 24.495640071324075 21.48193108600243 80-81 20.829414602801922 33.29686968470061 24.50379422051169 21.36992149198578 82-83 21.137949281194558 32.72043292853744 24.31754614539747 21.824071644870536 84-85 21.213037484992665 32.187736226599675 24.865712125928233 21.733514162479434 86-87 21.14746262019899 32.444222111055524 24.50425212606303 21.904063142682453 88-89 21.40055673642177 31.823133910228474 24.92040270008271 21.855906653267045 90-91 21.458000280153776 32.161430829811025 24.426740900005115 21.953827990030085 92-93 21.505142031241313 32.125854688976595 24.408916560120073 21.960086719662016 94-95 21.276179465516478 32.10191649250745 24.581795544488415 22.040108497487658 96-97 20.967139490417537 32.700431321979636 24.327004313219796 22.00542487438303 98-99 20.12348588605681 32.76711549065296 24.017715800145854 23.091682823144378 100-101 19.714082440304146 34.17759793676909 24.281648806082973 21.82667081684379 102-103 19.601404245195976 35.18905951589558 23.81236118182485 21.39717505708359 104-105 18.5887401609819 36.17089874149508 23.995197225063368 21.245163872459642 106-107 18.16488060829739 37.37738449908844 23.23313619991996 21.224598692694205 108-109 17.989906176352882 38.35786384454622 22.59349015074036 21.058739828360533 110-111 17.96338621891786 39.03320764113692 22.551670164704205 20.451735975241007 112-113 18.23152212098169 39.46312873259604 22.168118571485746 20.13723057493652 114-115 17.887832805291534 39.980879328553165 21.838697126340946 20.29259073981435 116-117 17.912757348036816 39.90484236737961 22.036551202810262 20.145849081773314 118-119 17.993494264680706 39.903103231300456 21.97720102188515 20.126201482133684 120-121 17.86816431436372 39.61962950116948 22.03005078128474 20.482155403182055 122-123 18.37613583559926 39.34132095984917 21.806931848752388 20.47561135579918 124-125 18.29161382195225 39.76837327281223 21.49366975947311 20.44634314576241 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1741.0 1 1567.0 2 1633.5 3 1459.5 4 799.5 5 447.0 6 309.0 7 313.0 8 385.5 9 394.0 10 291.0 11 188.5 12 172.0 13 176.5 14 183.0 15 189.5 16 199.5 17 251.0 18 317.5 19 391.5 20 500.0 21 594.5 22 715.5 23 888.5 24 1044.5 25 1269.5 26 1624.0 27 2089.5 28 2555.0 29 2922.0 30 3397.5 31 3965.5 32 4652.5 33 5328.0 34 6224.5 35 6685.5 36 7063.5 37 8177.5 38 8288.5 39 7695.5 40 7479.0 41 7244.5 42 7079.5 43 7048.0 44 7010.0 45 7025.5 46 6884.5 47 6491.0 48 6286.5 49 6234.0 50 6017.0 51 5642.0 52 5140.0 53 4876.5 54 4589.0 55 4370.5 56 4293.0 57 3883.5 58 3931.0 59 4100.0 60 4201.0 61 4337.5 62 3833.5 63 2447.0 64 1352.5 65 1183.5 66 952.5 67 684.5 68 589.5 69 554.5 70 514.0 71 439.0 72 350.0 73 385.5 74 324.5 75 245.5 76 205.0 77 123.5 78 83.0 79 49.5 80 36.0 81 31.5 82 20.5 83 11.0 84 7.5 85 6.0 86 3.0 87 4.0 88 5.0 89 3.0 90 6.5 91 9.0 92 3.5 93 1.5 94 3.5 95 5.0 96 3.5 97 2.0 98 2.5 99 5.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018231135221663922 2 0.007114589354795677 3 0.006669927520120948 4 0.02890301925385744 5 0.025345724576459602 6 0.023122415403085954 7 0.011116545866868248 8 4.4466183467472984E-4 9 0.012895193205567167 10-11 0.0 12-13 0.003112632842723109 14-15 0.015118502378940815 16-17 6.669927520120948E-4 18-19 0.009337898528169327 20-21 0.0022233091733736493 22-23 2.2233091733736492E-4 24-25 0.0057806038507714885 26-27 0.0 28-29 0.004891280181422029 30-31 6.669927520120948E-4 32-33 0.0011116545866868247 34-35 2.2233091733736492E-4 36-37 0.006225265685446218 38-39 6.669927520120948E-4 40-41 0.013339855040241896 42-43 0.006002934768108853 44-45 0.0 46-47 0.010004891280181423 48-49 0.002667971008048379 50-51 0.008893236693494597 52-53 0.004001956512072569 54-55 0.002667971008048379 56-57 0.009782560362844058 58-59 0.01756414246965183 60-61 0.0015563164213615545 62-63 0.0068922584374583136 64-65 0.0011116545866868247 66-67 6.669927520120948E-4 68-69 2.2233091733736492E-4 70-71 0.0057806038507714885 72-73 2.2233091733736492E-4 74-75 0.005335942016096758 76-77 0.012895193205567167 78-79 4.4466183467472984E-4 80-81 0.003779625594735204 82-83 0.0013339855040241896 84-85 0.0 86-87 0.0011116545866868247 88-89 0.0035572946773978387 90-91 0.006002934768108853 92-93 0.01222820045355507 94-95 0.0 96-97 0.0 98-99 0.002667971008048379 100-101 0.0 102-103 2.2233091733736492E-4 104-105 0.008893236693494597 106-107 0.0 108-109 0.0 110-111 8.893236693494597E-4 112-113 0.0057806038507714885 114-115 0.0011116545866868247 116-117 0.0 118-119 0.005113611098759394 120-121 0.0017786473386989193 122-123 2.2233091733736492E-4 124-125 0.07781582106807772 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 224890.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.690818922112314 #Duplication Level Percentage of deduplicated Percentage of total 1 73.24286637110036 34.93012277440617 2 11.915425217120445 11.365127728193238 3 5.083523291146315 7.273121662931981 4 2.711349715469112 5.172259532598327 5 1.6761751853407967 3.9969083622912995 6 1.1299498840429987 3.233294118657387 7 0.8310062146269803 2.774195683344772 8 0.6277365966811551 2.3949817890483214 9 0.45336508909155854 1.945921713252555 >10 2.179141432269461 17.7204784692994 >50 0.09964066874052861 3.1039705329073577 >100 0.04483830093323788 3.7041411977974357 >500 0.003985626749621145 1.2635421994795872 >1k 9.964066874052861E-4 1.1219342357921418 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2361 1.0498465916670372 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 736 0.3272711103206012 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 690 0.3068166659255636 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 650 0.2890301925385744 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 583 0.2592378496153675 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 463 0.2058784294543999 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 417 0.18542398505936233 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 370 0.16452487882965006 No Hit CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT 339 0.15074036195473342 No Hit CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT 281 0.1249499755435991 No Hit GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT 277 0.12317132820490018 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 260 0.11561207701542976 No Hit CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT 256 0.11383342967673084 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 247 0.10983147316465827 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.446618346747299E-4 0.0 10-11 0.0 0.0 0.0 4.446618346747299E-4 0.0 12-13 0.0 0.0 0.0 4.446618346747299E-4 0.0 14-15 0.0 0.0 0.0 6.669927520120949E-4 0.0 16-17 0.0 0.0 0.0 8.893236693494598E-4 0.0 18-19 0.0 0.0 0.0 0.0013339855040241896 0.0 20-21 0.0 0.0 0.0 0.0017786473386989196 0.0 22-23 0.0 0.0 0.0 0.007781582106807773 0.0 24-25 0.0 0.0 0.0 0.018231135221663926 0.0 26-27 0.0 0.0 0.0 0.03446129218729156 0.0 28-29 0.0 0.0 0.0 0.060251678598425895 0.0 30-31 0.0 0.0 0.0 0.1387344924185157 0.0 32-33 0.0 0.0 0.0 0.23300280136955842 0.0 34-35 0.0 0.0 0.0 0.35661879140913333 0.0 36-37 0.0 0.0 0.0 0.5211436702387834 0.0 38-39 0.0 0.0 0.0 0.8021699497532127 0.0 40-41 0.0 0.0 0.0 1.1705722797812266 0.0 42-43 0.0 0.0 0.0 1.4453732936102095 0.0 44-45 0.0 0.0 0.0 1.696162568366757 0.0 46-47 0.0 0.0 0.0 1.981190804393259 0.0 48-49 0.0 0.0 0.0 2.2922317577482323 0.0 50-51 0.0 0.0 0.0 2.67886522299791 0.0 52-53 0.0 0.0 0.0 3.0270354395482233 0.0 54-55 0.0 0.0 0.0 3.327626839788341 0.0 56-57 0.0 0.0 0.0 3.677353372760016 0.0 58-59 0.0 0.0 0.0 4.070879096447152 0.0 60-61 0.0 0.0 0.0 4.54688959046645 0.0 62-63 0.0 0.0 0.0 4.99577571257059 0.0 64-65 0.0 0.0 0.0 5.398417003868558 0.0 66-67 0.0 0.0 0.0 5.832406954511095 0.0 68-69 0.0 0.0 0.0 6.326426252834719 0.0 70-71 0.0 0.0 0.0 6.919605140290809 0.0 72-73 0.0 0.0 0.0 7.543688025256793 0.0 74-75 0.0 0.0 0.0 8.09551336208813 0.0 76-77 0.0 0.0 0.0 8.63733380763929 0.0 78-79 0.0 0.0 0.0 9.297434301213926 0.0 80-81 0.0 0.0 0.0 10.031348659344568 0.0 82-83 0.0 0.0 0.0 10.848637111476723 0.0 84-85 0.0 0.0 0.0 11.560540708790963 0.0 86-87 0.0 0.0 0.0 12.25287918537952 0.0 88-89 0.0 0.0 0.0 13.011472275334608 0.0 90-91 0.0 0.0 0.0 13.837431633242918 0.0 92-93 0.0 0.0 0.0 14.7536573435902 0.0 94-95 0.0 0.0 0.0 15.63453243808084 0.0 96-97 0.0 0.0 0.0 16.494508426341767 0.0 98-99 0.0 0.0 0.0 17.357597047445417 0.0 100-101 0.0 0.0 0.0 18.30761705722798 0.0 102-103 0.0 0.0 0.0 19.349459735870873 0.0 104-105 0.0 0.0 0.0 20.36840233002801 0.0 106-107 0.0 0.0 0.0 21.348437013651118 0.0 108-109 0.0 0.0 0.0 22.328249366356886 0.0 110-111 0.0 0.0 0.0 23.392102805816176 0.0 112-113 0.0 0.0 0.0 24.49819911956957 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATAGT 25 0.0020514734 71.42818 6 GGTATCA 610 0.0 71.26475 1 GTATCAA 1340 0.0 65.771645 1 GTACAAG 175 0.0 57.848568 1 CGTCACT 55 1.3008123E-5 54.136364 1 ACAGTAC 45 3.1592694E-4 52.909763 8 CGCAGAA 60 2.1768574E-5 49.625 2 ATCAACG 1675 0.0 44.4403 3 TCAACGC 1695 0.0 43.915928 4 CAACGCA 1795 0.0 41.46936 5 TATCAAC 2095 0.0 41.21599 2 TATAGGG 60 0.0013008824 39.7 2 GTACATG 3795 0.0 38.915413 1 TACATGG 3840 0.0 37.528904 2 AACGCAG 2015 0.0 36.92524 6 GTACATA 120 9.183732E-7 34.7375 1 GAACAAA 415 0.0 34.438557 1 GAGAACA 840 0.0 34.02857 1 GTCACTA 105 1.4699142E-5 34.02857 2 TACAAGG 195 3.8198777E-11 33.592304 2 >>END_MODULE