FastQCFastQC Report
Fri 27 May 2016
SRR940095_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940095_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224890
Sequences flagged as poor quality0
Sequence length125
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50222.2330917337364933No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40761.812441638134199No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23491.0445106496509404No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT10510.46733958824314115No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7970.35439548223575973No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7860.3495042020543377No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7670.3410556271955178No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7220.32104584463515495No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7090.3152652407843835No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6510.2894748543732491No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT5980.26590777713548847RNA PCR Primer, Index 23 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5790.2574592022766686No Hit
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG5380.23922806705500466RNA PCR Primer, Index 23 (96% over 30bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5320.2365600960469563No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5120.22766685935346168No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5070.22544355018008802No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT4730.21032504780114722No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC4650.2067677531237494No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG4630.2058784294543999No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4550.20232113477700206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG4310.1916492507448086No Hit
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT3500.15563164213615543RNA PCR Primer, Index 23 (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG3320.1476277291120103No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC3130.13917915425319044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC3000.13339855040241896No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC2920.12984125572502112No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.12850727022099695No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT2810.1249499755435991No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2740.12183734270087597No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT2670.11872470985815288No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2660.11828004802347815No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2650.1178353861888034No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA2580.11472275334608031No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT2350.10449553114856153No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC2340.10405086931388678No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA2280.10138289830583841No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18600.093.868651
GTATCAA41050.064.887381
GGTGCGA300.00422946559.5094536
CCTCGTC150.004235943359.4962222-23
GTGCGAG350.007790103650.996767
GCGAGCG350.007790103650.996769
ATCAACG55600.047.789153
AGATAGT505.3192285E-447.6075636
CGTGGGC255.330788E-447.59697748-49
GCGAATC255.330788E-447.59697722-23
CATTCCG505.325067E-447.5969779
CGATCCG255.330788E-447.59697770-71
TCAACGC56050.047.4054684
TATCAAC56700.046.967092
CAACGCA57900.045.849955
AACGCAG60150.044.2239846
GTGGTAT3800.043.9076651
GTCCAAG558.4513205E-443.3374371
GGAACGA558.516415E-443.2699788
TAGTCAA558.516415E-443.2699789
Produced by FastQC (version 0.11.5)