FastQCFastQC Report
Fri 27 May 2016
SRR940094_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940094_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366739
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17910.4883582056994211No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4580.12488445461213561No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4240.1156135562348155No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3940.10743335178423892No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5050.081.361461
GTATCAA11950.064.281031
CGATGCA300.004230124459.5146649
CAGTGCG453.164224E-452.9019289
TCAACGC15550.045.5698474
TATCAAC16900.044.3961032
ATCAACG16100.044.3829733
CAACGCA16600.043.763575
GTAGTTT558.5104693E-443.283398
TTGCGCA355.4552682E-542.49308896-97
GTACATG30950.041.7503471
AACGCAG16750.041.582786
GTACAAG2200.037.8936421
TAGATAG801.1903142E-437.2169725
TACATGG34000.036.954262
TAAGGGT650.00192965936.64443
GATTGGC851.7057435E-435.008639
ACGCAGA20450.034.0360267
CTAAGGG700.002775717834.0269472
AAGGGTA700.002775717834.0269474
Produced by FastQC (version 0.11.5)