FastQCFastQC Report
Fri 27 May 2016
SRR940094_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940094_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366739
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36590.9977122694886554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30420.829472731288464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17490.47690591946861394No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT11470.31275648349371077No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6680.1821458857661716No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6470.17641974265076799No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT5570.1518791292990383RNA PCR Primer, Index 39 (95% over 23bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5420.14778902707375No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5390.14697100662869234No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG5370.14642565966532056RNA PCR Primer, Index 39 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4560.12433910764876384No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4130.11261414793627075No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT4070.11097810704615543RNA PCR Primer, Index 39 (96% over 25bp)
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4050.11043276008278367No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3860.10525196393075184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG3790.10334324955895065No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTACG152.7014327E-4118.986639
GGTATCA14250.099.041311
GTATCAA32850.065.079161
TTACGAG150.00423782559.49331710-11
TATCAAC45300.047.180292
GTGGTAT3300.046.918311
ATCAACG45750.046.716223
TCAACGC46350.046.111484
CAACGCA47200.045.2563865
AACGCAG48350.044.179976
GTTATAC853.4543064E-642.0354041
CGGGTTG300.001306294539.678444112-113
ACGCAGA55300.038.6222467
CGCAGAG56400.037.8689778
GCAGAGT58250.036.257739
TGGTATC4450.034.7838552
GTGGTAC700.00278619434.0008166
GTATAGT1451.0862641E-732.855261
GGGATCC750.00390919231.7297737
GGATATG953.2658048E-431.342191
Produced by FastQC (version 0.11.5)