##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940094_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 366739 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.60086601097783 34.0 31.0 34.0 31.0 34.0 2 32.70034275056648 34.0 31.0 34.0 31.0 34.0 3 32.75679706821472 34.0 31.0 34.0 31.0 34.0 4 36.21595194402559 37.0 35.0 37.0 35.0 37.0 5 36.211054728294506 37.0 37.0 37.0 35.0 37.0 6 36.292886766883264 37.0 37.0 37.0 35.0 37.0 7 36.236985976402835 37.0 37.0 37.0 35.0 37.0 8 36.24669860582049 37.0 37.0 37.0 35.0 37.0 9 38.03407327827147 39.0 39.0 39.0 35.0 39.0 10-11 38.01330646590627 39.0 39.0 39.0 35.0 39.0 12-13 38.017369300783386 39.0 39.0 39.0 35.0 39.0 14-15 39.39151685531127 41.0 39.0 41.0 36.0 41.0 16-17 39.39623956001407 41.0 39.0 41.0 36.0 41.0 18-19 39.49958553630784 41.0 39.5 41.0 36.0 41.0 20-21 39.4950986941667 41.0 39.5 41.0 36.0 41.0 22-23 39.42127643910247 41.0 39.0 41.0 36.0 41.0 24-25 39.364021824785475 41.0 39.0 41.0 35.5 41.0 26-27 39.18162507941615 41.0 39.0 41.0 35.0 41.0 28-29 39.02966142133779 41.0 39.0 41.0 35.0 41.0 30-31 38.712795748475074 40.0 38.0 41.0 35.0 41.0 32-33 38.33476259683317 40.0 37.0 41.0 35.0 41.0 34-35 38.08473873790352 40.0 37.0 41.0 34.0 41.0 36-37 37.85599568085205 40.0 37.0 41.0 34.0 41.0 38-39 37.602303000226314 40.0 36.0 41.0 33.0 41.0 40-41 37.375989463896666 40.0 36.0 41.0 33.0 41.0 42-43 37.06380695808191 40.0 36.0 41.0 32.5 41.0 44-45 36.86332105393754 40.0 36.0 41.0 31.5 41.0 46-47 36.19994601065063 40.0 36.0 41.0 28.5 41.0 48-49 35.529920461145394 40.0 35.0 41.0 23.5 41.0 50-51 35.204657808414154 40.0 35.0 41.0 20.5 41.0 52-53 35.05595259844195 40.0 35.0 41.0 20.0 41.0 54-55 34.87020333261529 40.0 35.0 41.0 20.0 41.0 56-57 34.692838776350484 39.0 35.0 41.0 18.5 41.0 58-59 34.45785013320099 39.0 35.0 41.0 18.0 41.0 60-61 34.17866111867023 39.0 35.0 41.0 13.5 41.0 62-63 33.90807767922146 38.0 35.0 41.0 9.5 41.0 64-65 33.61571853552526 37.5 34.5 41.0 7.5 41.0 66-67 33.27433269982194 37.0 34.0 40.0 3.5 41.0 68-69 32.90301958613619 36.5 34.0 39.5 2.0 41.0 70-71 32.59507442622683 36.0 34.0 39.0 2.0 41.0 72-73 32.39470986178181 36.0 34.0 39.0 2.0 41.0 74-75 32.09684544049038 35.0 34.0 38.5 2.0 40.5 76-77 31.785216461843437 35.0 34.0 37.0 2.0 39.5 78-79 31.471625597495766 35.0 34.0 37.0 2.0 39.0 80-81 31.19408216742697 35.0 34.0 36.0 2.0 39.0 82-83 30.929193513643217 35.0 33.5 36.0 2.0 37.0 84-85 30.705447198143638 35.0 33.0 36.0 2.0 37.0 86-87 30.51465483627321 35.0 33.0 35.0 2.0 36.5 88-89 30.359023719866173 35.0 33.0 35.0 2.0 36.0 90-91 30.23918099793041 35.0 33.0 35.0 2.0 36.0 92-93 30.118599330859276 35.0 33.0 35.0 2.0 36.0 94-95 30.012676590163576 35.0 33.0 35.0 2.0 36.0 96-97 29.928687704334692 35.0 33.0 35.0 2.0 35.0 98-99 29.871442633589552 35.0 33.0 35.0 2.0 35.0 100-101 29.835805572900618 35.0 33.0 35.0 2.0 35.0 102-103 29.086674719623492 34.0 30.5 35.0 2.0 35.0 104-105 29.660858267050955 35.0 32.5 35.0 2.0 35.0 106-107 29.687513463253158 35.0 33.0 35.0 2.0 35.0 108-109 29.65989845639542 35.0 33.0 35.0 2.0 35.0 110-111 29.587380943941056 35.0 33.0 35.0 2.0 35.0 112-113 29.48814006691407 35.0 33.0 35.0 2.0 35.0 114-115 29.367124849007062 35.0 33.0 35.0 2.0 35.0 116-117 29.22406534347315 35.0 32.0 35.0 2.0 35.0 118-119 29.097968855234924 35.0 32.0 35.0 2.0 35.0 120-121 28.92082107438805 35.0 32.0 35.0 2.0 35.0 122-123 28.705440381306598 35.0 32.0 35.0 2.0 35.0 124-125 26.992035207599955 33.0 27.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3584747527236374 1101 2 0.4296189998792528 1101 3 0.32976670622958437 1101 4 0.15600324010140554 1101 5 0.18866273467383365 1101 6 0.2350397671119282 1101 7 0.2824747257478606 1101 8 0.23127418471946726 1101 9 0.2790877583282665 1101 10-11 0.35540443729641424 1101 12-13 0.2782682363249265 1101 14-15 0.36530828973305773 1101 16-17 0.4611434514868691 1101 18-19 0.41767169557929407 1101 20-21 0.42098808135980903 1101 22-23 0.458564245587759 1101 24-25 0.4425660264324023 1101 26-27 0.4353107807865868 1101 28-29 0.4642666290638502 1101 30-31 0.3352718651343096 1101 32-33 0.6225983518125204 1101 34-35 0.7155276005604847 1101 36-37 0.7368989494543356 1101 38-39 0.731537782934069 1101 40-41 0.7103562127535952 1101 42-43 0.7610251115295839 1101 44-45 0.7625076380563627 1101 46-47 0.9654073761109032 1101 48-49 0.7791808018055164 1101 50-51 0.9849494996334656 1101 52-53 0.7147618757997449 1101 54-55 0.8946580177933754 1101 56-57 0.9976502514281762 1101 58-59 0.8294429764314444 1101 60-61 0.8521379440710675 1101 62-63 0.8715224791177576 1101 64-65 0.874765878156218 1101 66-67 0.9383153737849455 1101 68-69 1.1598603359659165 1101 70-71 1.0699707979800053 1101 72-73 0.8951086260118615 1101 74-75 1.005695668179989 1101 76-77 1.011246117610515 1101 78-79 1.059354742775728 1101 80-81 1.1115431971399765 1101 82-83 1.0935212160459358 1101 84-85 1.1751590733477002 1101 86-87 1.0428771328042785 1101 88-89 1.05878136109963 1101 90-91 1.0754222439602366 1101 92-93 1.0307811861584248 1101 94-95 1.1351433929210728 1101 96-97 1.0408450128818778 1101 98-99 1.0269553234995854 1101 100-101 1.0538229589698886 1101 102-103 1.0543103482579248 1101 104-105 1.0865342449423387 1101 106-107 1.103793457266601 1101 108-109 1.1649151459270222 1101 110-111 1.0947748350476871 1101 112-113 1.1883212813955808 1101 114-115 1.2678889960992272 1101 116-117 1.3268314432831474 1101 118-119 1.2184715096331402 1101 120-121 1.2948509149068066 1101 122-123 1.3666621644144286 1101 124-125 1.538061274838256 1102 1 0.22335487847332303 1102 2 0.2825803745797586 1102 3 0.3041589414913872 1102 4 0.04456062865879318 1102 5 0.01861832549899134 1102 6 0.0343743925335076 1102 7 0.06963116507821354 1102 8 0.055201726038312415 1102 9 0.13864946571606396 1102 10-11 0.20648109272089954 1102 12-13 0.1810792913098922 1102 14-15 0.2897707250650612 1102 16-17 0.3532871479783921 1102 18-19 0.3235959710687766 1102 20-21 0.3063673906521629 1102 22-23 0.2844757019339994 1102 24-25 0.2993464375606365 1102 26-27 0.3994477014452471 1102 28-29 0.30942587639700747 1102 30-31 0.34289467710495103 1102 32-33 0.36933633768094154 1102 34-35 0.44637900532493546 1102 36-37 0.551784416513712 1102 38-39 0.6496936576116923 1102 40-41 0.7999681284524698 1102 42-43 0.6180115446029717 1102 44-45 0.5393858714563322 1102 46-47 0.38694353677749405 1102 48-49 0.20658858790896062 1102 50-51 0.3460487411674862 1102 52-53 0.43539993339432215 1102 54-55 0.44090231403767177 1102 56-57 0.2924066114019226 1102 58-59 0.30699421485226 1102 60-61 0.45421800267286727 1102 62-63 0.21475503974162535 1102 64-65 0.2766002365123157 1102 66-67 0.07480491244839271 1102 68-69 0.29153888068793776 1102 70-71 0.25729815922041155 1102 72-73 0.09276379105832433 1102 74-75 0.12948815066811648 1102 76-77 0.3383479229731883 1102 78-79 0.2554265910214859 1102 80-81 0.18009509177883487 1102 82-83 0.24213934292493278 1102 84-85 0.18211803682840255 1102 86-87 0.17545225450983537 1102 88-89 0.2404316731847267 1102 90-91 0.32278211088531705 1102 92-93 0.1886474092420407 1102 94-95 0.2275633114279465 1102 96-97 0.2838211906406656 1102 98-99 0.3204717365812151 1102 100-101 0.23315361656141675 1102 102-103 0.24365977673778616 1102 104-105 0.3564191778707517 1102 106-107 0.24710079622611403 1102 108-109 0.36831221888931154 1102 110-111 0.5262153099664211 1102 112-113 0.5070906436432026 1102 114-115 0.45388484513854976 1102 116-117 0.277634165824999 1102 118-119 0.43738127202116317 1102 120-121 0.46376601860452027 1102 122-123 0.6148690104473609 1102 124-125 0.7522592390362206 1103 1 0.17730052680232689 1103 2 0.2030291526668364 1103 3 0.04834159017411821 1103 4 0.06171662441401082 1103 5 -0.004620320476924178 1103 6 0.05246085690655633 1103 7 0.13286458197429596 1103 8 0.13393782668271825 1103 9 0.2505133250845759 1103 10-11 0.12884463680665448 1103 12-13 0.1260778302434744 1103 14-15 0.34506055450477646 1103 16-17 0.4523397617493288 1103 18-19 0.48634723604471475 1103 20-21 0.3652176082741576 1103 22-23 0.44813052114625407 1103 24-25 0.5132734765172842 1103 26-27 0.40246595708573096 1103 28-29 0.46047323032881593 1103 30-31 0.4856331769742255 1103 32-33 0.674057120197368 1103 34-35 0.8318121730707375 1103 36-37 0.5733659055710874 1103 38-39 0.5954073501110315 1103 40-41 0.5701769840529707 1103 42-43 0.5137067815264302 1103 44-45 0.4908477068633559 1103 46-47 0.5139140539950091 1103 48-49 0.827668700487969 1103 50-51 0.6705631288268634 1103 52-53 0.8134278013139138 1103 54-55 0.633244019025291 1103 56-57 0.7600670595648253 1103 58-59 0.5918560287277685 1103 60-61 0.7556090917056437 1103 62-63 0.8207284944716378 1103 64-65 0.654485842701078 1103 66-67 0.9383566880285983 1103 68-69 0.7607872773597819 1103 70-71 0.7232053276736821 1103 72-73 0.45510441947069324 1103 74-75 0.5787430067915267 1103 76-77 0.6111251795518307 1103 78-79 0.5773264387629844 1103 80-81 0.49947267748191493 1103 82-83 0.4247149723369681 1103 84-85 0.4235515736857245 1103 86-87 0.44143939712529345 1103 88-89 0.4173514375685379 1103 90-91 0.39816160366457254 1103 92-93 0.25508748244915935 1103 94-95 0.4391411093701514 1103 96-97 0.5868540569453948 1103 98-99 0.6034918377792877 1103 100-101 0.5270663524467452 1103 102-103 0.3816844149316019 1103 104-105 0.370945254061521 1103 106-107 0.3816880834102534 1103 108-109 0.38665244743085836 1103 110-111 0.4321995304490365 1103 112-113 0.5432481927427268 1103 114-115 0.4871587105051276 1103 116-117 0.5970040617047943 1103 118-119 0.751395205980959 1103 120-121 0.7967776609969768 1103 122-123 0.7130257598052623 1103 124-125 0.8278694265141127 1104 1 0.09784986848251265 1104 2 0.04180182054915349 1104 3 0.18385352895109008 1104 4 -0.03997476214966156 1104 5 -0.06210305483734402 1104 6 0.15214928569746178 1104 7 0.01937082057753514 1104 8 0.1485902883824366 1104 9 0.14278434896212389 1104 10-11 0.21372625410531754 1104 12-13 0.1682225026629638 1104 14-15 0.22221606730503396 1104 16-17 0.3248899074842484 1104 18-19 0.15974666459153042 1104 20-21 0.15160826400951777 1104 22-23 0.12771112045418676 1104 24-25 0.15001662410439565 1104 26-27 0.15817689940916324 1104 28-29 0.20817593109123322 1104 30-31 0.0553562088348869 1104 32-33 0.12684403133495437 1104 34-35 0.13412239461653286 1104 36-37 0.24028623236191038 1104 38-39 0.2819552272629551 1104 40-41 0.16204023731584982 1104 42-43 -0.013458781958000543 1104 44-45 0.12468333510290108 1104 46-47 0.23737306874079422 1104 48-49 0.35475888384116416 1104 50-51 0.0727125394260284 1104 52-53 0.1413564988888325 1104 54-55 0.2631096270198938 1104 56-57 0.133640221366484 1104 58-59 0.006967232368985776 1104 60-61 -0.08108164874261803 1104 62-63 -0.2306017853681226 1104 64-65 0.0746618732551596 1104 66-67 0.11889210233214698 1104 68-69 -0.006668607315795327 1104 70-71 -0.15526036359801765 1104 72-73 -0.14184160835534243 1104 74-75 -0.10736335816317322 1104 76-77 -0.24802884000392922 1104 78-79 -0.16928863439163777 1104 80-81 -0.2932068349163224 1104 82-83 -0.2217952865399404 1104 84-85 -0.216985707173464 1104 86-87 -0.34027051141347897 1104 88-89 -0.363414424564791 1104 90-91 -0.35784009059361566 1104 92-93 -0.4733896953187262 1104 94-95 -0.49867387189250323 1104 96-97 -0.4414278503872957 1104 98-99 -0.5118613001878884 1104 100-101 -0.6020490437655823 1104 102-103 -0.5759878466159165 1104 104-105 -0.42845385860665175 1104 106-107 -0.369503640569242 1104 108-109 -0.4058188722239713 1104 110-111 -0.34178929893342413 1104 112-113 -0.24543258317009276 1104 114-115 -0.23898886225464722 1104 116-117 -0.5167337877359586 1104 118-119 -0.471633065157782 1104 120-121 -0.4310569908903972 1104 122-123 -0.46301556286477563 1104 124-125 -0.43660355868266265 1105 1 -0.17254197829309703 1105 2 -0.166442776182528 1105 3 -0.13043921397633795 1105 4 -0.01673807263991023 1105 5 -0.29113134512024885 1105 6 -7.131286409673976E-4 1105 7 -0.1930979898248495 1105 8 0.06090095434623777 1105 9 -0.11957376033325318 1105 10-11 -0.04118500929303792 1105 12-13 0.06068779874448893 1105 14-15 -0.06595297152820478 1105 16-17 -0.13615384581042633 1105 18-19 0.0011980791056700468 1105 20-21 -0.06607755570582441 1105 22-23 -0.05451168748817281 1105 24-25 0.11808726195363306 1105 26-27 0.10563253110833415 1105 28-29 -0.06161496681774992 1105 30-31 -0.07996623010378556 1105 32-33 0.0675275667417381 1105 34-35 -0.08870663367374476 1105 36-37 -0.04743824488286208 1105 38-39 -2.2540882911670224E-4 1105 40-41 -0.19399385159646698 1105 42-43 -0.1969028163983424 1105 44-45 -0.26252453697581757 1105 46-47 -0.3236531129495859 1105 48-49 -0.3038951312704157 1105 50-51 -0.04021137552741294 1105 52-53 -0.10666669562882447 1105 54-55 -0.040785997650637285 1105 56-57 -0.19790959413167286 1105 58-59 0.037937184925660006 1105 60-61 -0.24271405078092556 1105 62-63 -0.2114504938552102 1105 64-65 -0.3292495658592287 1105 66-67 -0.1300887446191794 1105 68-69 -0.06353734743176886 1105 70-71 -0.19757102956181427 1105 72-73 -0.08006511916187975 1105 74-75 -0.33064924816493146 1105 76-77 -0.13059429422989766 1105 78-79 -0.1731034296824454 1105 80-81 0.06474783034461495 1105 82-83 0.004885437410155902 1105 84-85 -0.018277220088592117 1105 86-87 -0.009040499113353917 1105 88-89 -0.07318775086948293 1105 90-91 -0.04372833519034458 1105 92-93 -0.11400645862921621 1105 94-95 -0.14094412316644522 1105 96-97 -0.18274572070885498 1105 98-99 -0.053701047156785364 1105 100-101 -0.034143578996648216 1105 102-103 -0.0818286478810748 1105 104-105 -0.09385185544376284 1105 106-107 -0.002808859335715397 1105 108-109 0.18452904554092342 1105 110-111 0.17046338073623346 1105 112-113 -0.13978682671252685 1105 114-115 -0.263269305059076 1105 116-117 -0.29844526199356025 1105 118-119 -0.10174728058565208 1105 120-121 -0.1182121481562568 1105 122-123 -0.3086788909266289 1105 124-125 -0.07323859646161424 1106 1 0.16796056958952477 1106 2 0.07685717110265955 1106 3 0.13219301234592962 1106 4 0.046741105009012074 1106 5 -0.0012174459812541727 1106 6 0.15030426724727874 1106 7 0.09870661393546953 1106 8 0.08770467952634675 1106 9 0.2866957880765213 1106 10-11 0.13439046074737604 1106 12-13 0.06490146945262865 1106 14-15 0.058009425238616075 1106 16-17 0.10441020268719825 1106 18-19 0.05458061293101224 1106 20-21 0.1377706256331308 1106 22-23 -0.06970585371555416 1106 24-25 0.16754429938114868 1106 26-27 0.07146103225048961 1106 28-29 0.20079585729049398 1106 30-31 0.25461820145481795 1106 32-33 0.2772130350249924 1106 34-35 0.33615191491173846 1106 36-37 0.3841976714763007 1106 38-39 0.2570474781854699 1106 40-41 0.05133913030477544 1106 42-43 0.2798991516213363 1106 44-45 0.35069809525050744 1106 46-47 0.32316642667437634 1106 48-49 0.7034673709260417 1106 50-51 0.4509413217138487 1106 52-53 0.5149727350511952 1106 54-55 0.6717812137357626 1106 56-57 0.7775516604808743 1106 58-59 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-1.9163223201527515 2216 92-93 -1.6882545789299677 2216 94-95 -1.6014955016125576 2216 96-97 -1.6748885778517142 2216 98-99 -1.612498039637991 2216 100-101 -1.6808854336332395 2216 102-103 -1.9549865426179132 2216 104-105 -1.9424232840151916 2216 106-107 -1.9863050999372192 2216 108-109 -2.07362884919592 2216 110-111 -2.025576719514394 2216 112-113 -2.1039973530283156 2216 114-115 -2.343419680998924 2216 116-117 -2.197680850965991 2216 118-119 -2.234967831964095 2216 120-121 -2.125668288507132 2216 122-123 -2.2397440538338955 2216 124-125 -2.512436591449081 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 1.0 4 16.0 5 45.0 6 103.0 7 253.0 8 511.0 9 959.0 10 1917.0 11 2581.0 12 3004.0 13 4558.0 14 8197.0 15 8602.0 16 2719.0 17 1962.0 18 1924.0 19 2032.0 20 1526.0 21 1816.0 22 1808.0 23 1280.0 24 1435.0 25 1752.0 26 2135.0 27 2530.0 28 3045.0 29 3893.0 30 4732.0 31 6026.0 32 7728.0 33 9995.0 34 14657.0 35 25689.0 36 50682.0 37 116994.0 38 69443.0 39 186.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.10004636827319 20.264571911736628 15.045413632272316 20.589968087717864 2 15.87830928768341 22.714617991836022 39.56884163683115 21.83823108364941 3 16.53455276239403 30.316391667961902 31.99501539283776 21.15404017680631 4 11.328606826691118 18.995535470631257 38.587615024396634 31.08824267828099 5 11.286658940623083 39.44099802304179 33.85343240848047 15.41891062785466 6 27.359036039426815 39.483273058523835 17.753249657715447 15.404441244333894 7 24.494258859843104 36.35500996621575 22.752966005797038 16.3977651681441 8 26.139843321817423 32.9463187716605 21.483943622030928 19.42989428449115 9 27.85495952162164 14.376709321888317 21.185093486103195 36.58323767038684 10-11 23.41692593370217 27.809832060402627 27.6935368204636 21.079705185431603 12-13 26.094443187116724 26.11612072891075 28.542914715915135 19.246521368057394 14-15 23.10785054221122 25.884211932736907 28.92547561072043 22.082461914331443 16-17 19.60781373129119 29.594616334777594 29.112529619156945 21.685040314774266 18-19 19.37113314918784 25.3308211016554 35.13084237018697 20.167203378969788 20-21 20.97867966046698 24.54034067824802 35.32144113388541 19.159538527399594 22-23 20.508045231077144 24.82392109920134 35.374067115850785 19.293966553870735 24-25 20.100235318497997 25.2551556019709 35.50841611290925 19.136192966621856 26-27 19.572911525635398 25.522374222539735 35.35307125776097 19.551642994063897 28-29 19.24780088237379 24.537408176954663 36.1405144817281 20.07427645894344 30-31 19.686979332837037 24.73487730320656 35.73286757908687 19.845275784869532 32-33 19.49997136928442 25.120589847275582 35.55716735880286 19.82227142463714 34-35 19.956699451108282 24.914994042084427 35.551441215687454 19.576865291119844 36-37 20.3883442198963 24.430074454001716 34.20603076050516 20.975550565596826 38-39 20.164749317634612 24.755480055298182 34.54064061907787 20.539130007989336 40-41 19.894802570765584 24.494395196583945 35.14024960530514 20.47055262734533 42-43 20.340487376581166 24.57292515930948 34.124540886025216 20.96204657808414 44-45 20.154447945397852 25.04529954966317 33.48894472841403 21.31130777652495 46-47 20.97433681044579 24.79061656825225 32.11273097620466 22.1223156450973 48-49 21.239219172217844 25.65380284071233 30.596827716714063 22.51015027035576 50-51 21.418978890378032 26.05619688679773 29.68640288387731 22.838421338946933 52-53 21.233629365843285 26.4445559376014 29.780715986028213 22.541098710527105 54-55 20.954783118485544 26.519056805709884 29.521654066656218 23.004506009148358 56-57 21.37351086194814 26.711094265949352 28.835629698504935 23.079765173597572 58-59 20.869957639192812 25.89149252652834 29.959875167186805 23.278674667092048 60-61 21.605146984640303 26.56316890213476 29.34566544599838 22.486018667226556 62-63 21.621201544468928 26.645633821251717 29.85823280469449 21.87493182958487 64-65 21.70338547454043 26.111687529057598 29.865690831228008 22.319236165173965 66-67 21.554595440620496 26.707858596793084 29.410465495168904 22.32708046741752 68-69 21.863879913288887 26.25533423316564 29.693238987279642 22.18754686626583 70-71 21.688542403754255 26.038057919916668 29.796948985699306 22.47645069062977 72-73 21.468762483622168 25.98297659998282 29.306943382129386 23.241317534265626 74-75 20.88828840128811 26.50154469527375 29.225552777315748 23.38461412612239 76-77 20.547289660503715 26.539090268300363 29.96336586822347 22.95025420297245 78-79 20.58047205619301 26.12683238078618 29.72533375507177 23.56736180794904 80-81 20.482742448287333 26.89767627025288 29.1000659871625 23.519515294297292 82-83 20.608581603215377 26.07160970502102 29.25153651925898 24.06827217250462 84-85 20.36148323467098 25.59722309326251 29.329850384060595 24.71144328800591 86-87 20.42292694898154 25.86548687044965 29.07561421209064 24.63597196847817 88-89 20.255257998456617 25.51707400873129 29.60826445830277 24.61940353450932 90-91 20.15469420421653 25.7173319953589 29.508744326447584 24.61922947397699 92-93 20.24563632843497 25.823309818635963 29.083963012753568 24.8470908401755 94-95 19.887600006544396 25.285226572427373 29.45264311775004 25.3745303032782 96-97 20.22400828953378 25.15587186671302 29.401531109611362 25.218588734141832 98-99 19.797449853295667 25.483333515123093 29.260833760539263 25.458382871041984 100-101 19.612433328606798 25.49001428867486 29.617232578178687 25.28031980453965 102-103 20.138984515919685 25.004669628870168 29.28573084122853 25.57061501398162 104-105 19.500782583561925 25.317805275758452 29.610562423991233 25.570849716688386 106-107 19.659349019329824 25.149356899593446 29.29890194388925 25.892392137187482 108-109 19.864391157747196 25.116950014520757 28.719051365291715 26.299607462440335 110-111 19.579832276483398 25.33985758089299 28.982001965999928 26.098308176623686 112-113 19.172288545546763 25.45688559726441 29.161326563445876 26.209499293742944 114-115 19.01938435781305 25.759600151606456 28.640804495840367 26.580210994740128 116-117 18.521644168801476 25.624389040147133 29.135337577047405 26.718629214003986 118-119 18.564605826269887 25.489487655271986 28.85703729254463 27.0888692259135 120-121 18.35356576084289 25.804661638553956 28.804078115712034 27.037694484891123 122-123 18.356704137211725 25.854676092247075 28.676351291472667 27.11226847906853 124-125 18.321762258746503 25.894018959285276 28.205824183318555 27.578394598649663 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 763.0 1 515.5 2 373.0 3 425.5 4 239.0 5 159.0 6 379.5 7 798.5 8 1240.0 9 1364.5 10 924.5 11 528.5 12 507.0 13 552.5 14 636.0 15 704.0 16 754.0 17 836.0 18 943.0 19 975.0 20 1032.0 21 1105.0 22 1155.0 23 1258.5 24 1348.0 25 1538.0 26 1845.0 27 2227.0 28 2601.5 29 3181.0 30 4203.0 31 5043.0 32 6154.5 33 7591.5 34 8864.0 35 10121.5 36 12457.0 37 14586.5 38 14827.0 39 14293.0 40 13577.0 41 13037.0 42 12619.0 43 13028.0 44 13626.0 45 13775.5 46 12805.5 47 11725.0 48 11849.5 49 11393.5 50 10511.0 51 9443.0 52 8730.5 53 8667.0 54 8450.5 55 8171.5 56 7538.5 57 6651.5 58 6351.5 59 6152.0 60 5454.5 61 4791.0 62 4175.0 63 3261.0 64 2407.5 65 1849.5 66 1432.5 67 1210.5 68 990.5 69 761.0 70 619.5 71 554.5 72 538.5 73 553.5 74 463.0 75 313.5 76 233.5 77 162.5 78 97.0 79 43.0 80 19.5 81 13.0 82 10.0 83 6.0 84 4.5 85 2.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029721409503761533 2 0.0016360408901153138 3 0.002726734816858856 4 0.08043867709733625 5 0.0038174287436023983 6 0.023177245943300274 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 5.453469633717712E-4 26-27 0.0 28-29 2.726734816858856E-4 30-31 0.0059988165970894835 32-33 0.0 34-35 0.0 36-37 6.81683704214714E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.003681092002759456 46-47 0.002045051112644142 48-49 0.0 50-51 0.002863071557701799 52-53 0.0 54-55 0.0017723776309582565 56-57 0.0 58-59 0.0023177245943300276 60-61 0.0 62-63 0.0029994082985447417 64-65 0.002863071557701799 66-67 1.363367408429428E-4 68-69 0.0010906939267435424 70-71 0.0023177245943300276 72-73 0.0023177245943300276 74-75 0.0 76-77 0.0012270306675864853 78-79 8.180204450576569E-4 80-81 2.726734816858856E-4 82-83 2.726734816858856E-4 84-85 0.0 86-87 0.0024540613351729705 88-89 0.0032720817802306275 90-91 0.004226438966131227 92-93 0.004908122670345941 94-95 0.003544755261916513 96-97 0.003135745039387684 98-99 0.004090102225288284 100-101 0.004090102225288284 102-103 0.002045051112644142 104-105 0.001363367408429428 106-107 0.0 108-109 0.006135153337932426 110-111 0.0012270306675864853 112-113 0.004635449188660055 114-115 0.0 116-117 1.363367408429428E-4 118-119 9.543571859005996E-4 120-121 2.726734816858856E-4 122-123 0.0017723776309582565 124-125 0.04471845099648524 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 366739.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.586884393799814 #Duplication Level Percentage of deduplicated Percentage of total 1 65.18708973374042 21.89431246855394 2 13.433065060278567 9.023496064679357 3 6.288577769219379 6.336412036085724 4 3.6797916364317356 4.943709451444166 5 2.4657779519931458 4.140889950718713 6 1.6239597677319366 3.272624938739673 7 1.1751839733575242 2.7629537778223843 8 0.9038546590774776 2.4286129554586067 9 0.7227436141629379 2.1847235593722885 >10 4.0716958990563645 25.14485758554269 >50 0.3199536492219146 7.392817360514475 >100 0.12018563473310498 6.9051450697710735 >500 0.00487239059728804 0.9518883079946696 >1k 0.003248260398192027 2.6175564733022476 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3659 0.9977122694886554 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3042 0.829472731288464 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1749 0.47690591946861394 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 1147 0.31275648349371077 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 668 0.1821458857661716 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 647 0.17641974265076799 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 557 0.1518791292990383 RNA PCR Primer, Index 39 (95% over 23bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 542 0.14778902707375 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 539 0.14697100662869234 No Hit TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG 537 0.14642565966532056 RNA PCR Primer, Index 39 (96% over 30bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 456 0.12433910764876384 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 413 0.11261414793627075 No Hit ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT 407 0.11097810704615543 RNA PCR Primer, Index 39 (96% over 25bp) GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 405 0.11043276008278367 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 386 0.10525196393075184 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 379 0.10334324955895065 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.726734816858856E-4 0.0 12-13 0.0 0.0 0.0 2.726734816858856E-4 0.0 14-15 0.0 0.0 0.0 4.090102225288284E-4 0.0 16-17 0.0 0.0 0.0 0.0012270306675864853 0.0 18-19 0.0 0.0 0.0 0.0019087143718011991 0.0 20-21 0.0 0.0 0.0 0.002045051112644142 0.0 22-23 0.0 0.0 0.0 0.003544755261916513 0.0 24-25 0.0 0.0 0.0 0.006816837042147141 0.0 26-27 0.0 0.0 0.0 0.012815653639236622 0.0 28-29 0.0 0.0 0.0 0.02903972579954682 0.0 30-31 0.0 0.0 0.0 0.0678956969397855 0.0 32-33 0.0 0.0 0.0 0.1248844546121356 0.0 34-35 0.0 0.0 0.0 0.20177837644755534 0.0 36-37 0.0 0.0 0.0 0.31207479978949604 0.0 38-39 0.0 0.0 0.0 0.4928573181472382 0.0 40-41 0.0 0.0 0.0 0.7217667060225392 0.0 42-43 0.0 0.0 0.0 0.9116837860167585 0.0 44-45 0.0 0.0 0.0 1.1190519688388747 0.0 46-47 0.0 0.0 0.0 1.351915122198621 0.0 48-49 0.0 0.0 0.0 1.6098642358734687 0.0 50-51 0.0 0.0 0.0 1.9057149635026545 0.0 52-53 0.0 0.0 0.0 2.1682995263661624 0.0 54-55 0.0 0.0 0.0 2.4322474566380996 0.0 56-57 0.0 0.0 0.0 2.723871745301154 0.0 58-59 0.0 0.0 0.0 3.0525796274734893 0.0 60-61 0.0 0.0 0.0 3.4480925126588664 0.0 62-63 0.0 0.0 0.0 3.8322894483542793 0.0 64-65 0.0 0.0 0.0 4.187037648027616 0.0 66-67 0.0 0.0 0.0 4.587867666105868 0.0 68-69 0.0 0.0 0.0 5.019782461096311 0.0 70-71 0.0 0.0 0.0 5.530772565775661 0.0 72-73 0.0 0.0 0.0 6.059077436542064 0.0 74-75 0.0 0.0 0.0 6.580429133525477 0.0 76-77 0.0 0.0 0.0 7.107234300142609 0.0 78-79 0.0 0.0 0.0 7.691164561172933 0.0 80-81 0.0 0.0 0.0 8.371757571460902 0.0 82-83 0.0 0.0 0.0 9.09107021614827 0.0 84-85 0.0 0.0 0.0 9.805611074906132 0.0 86-87 0.0 0.0 0.0 10.494247952903837 0.0 88-89 0.0 0.0 0.0 11.234011108717644 0.0 90-91 0.0 0.0 0.0 12.071255034234156 0.0 92-93 0.0 0.0 0.0 13.00038992307881 0.0 94-95 0.0 0.0 0.0 13.919299556360246 0.0 96-97 0.0 0.0 0.0 14.80712441272949 0.0 98-99 0.0 0.0 0.0 15.722216617267321 0.0 100-101 0.0 0.0 0.0 16.71883819282923 0.0 102-103 0.0 0.0 0.0 17.787036557333693 0.0 104-105 0.0 0.0 0.0 18.88509266808275 0.0 106-107 0.0 0.0 0.0 19.942111419838085 0.0 108-109 0.0 0.0 0.0 21.018626325533962 0.0 110-111 0.0 0.0 0.0 22.14149572311644 0.0 112-113 0.0 0.0 0.0 23.325989327559927 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTACG 15 2.7014327E-4 118.98663 9 GGTATCA 1425 0.0 99.04131 1 GTATCAA 3285 0.0 65.07916 1 TTACGAG 15 0.004237825 59.493317 10-11 TATCAAC 4530 0.0 47.18029 2 GTGGTAT 330 0.0 46.91831 1 ATCAACG 4575 0.0 46.71622 3 TCAACGC 4635 0.0 46.11148 4 CAACGCA 4720 0.0 45.256386 5 AACGCAG 4835 0.0 44.17997 6 GTTATAC 85 3.4543064E-6 42.035404 1 CGGGTTG 30 0.0013062945 39.678444 112-113 ACGCAGA 5530 0.0 38.622246 7 CGCAGAG 5640 0.0 37.868977 8 GCAGAGT 5825 0.0 36.25773 9 TGGTATC 445 0.0 34.783855 2 GTGGTAC 70 0.002786194 34.000816 6 GTATAGT 145 1.0862641E-7 32.85526 1 GGGATCC 75 0.003909192 31.729773 7 GGATATG 95 3.2658048E-4 31.34219 1 >>END_MODULE