FastQCFastQC Report
Fri 27 May 2016
SRR940093_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940093_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650069
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20000.059.8097761
GTATCAA35150.059.427561
TCAACGC43450.046.568824
ATCAACG43650.046.355453
CAACGCA45800.044.1807145
AACGCAG47350.042.729286
TATCAAC50150.041.2966162
GTACATG110150.037.495671
TACATGG110900.036.6517942
ACATGGG114050.034.595883
ACGCAGA61800.032.2520377
CGCAGAG61600.031.9723388
CATGGGG49650.029.8460064
CATGGGA72950.028.715974
GCAGAGT67000.027.708119
ATGGGAT28750.025.6686575
ATGGGGA23100.023.7025535
ATGGGAG31400.023.6918875
GAGTACA81000.023.584471
ATACCGA1552.1051751E-423.0348916
Produced by FastQC (version 0.11.5)