FastQCFastQC Report
Fri 27 May 2016
SRR940093_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940093_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650069
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46710.28307907123883913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35670.21617277822927405No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21520.13041878854763042No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33200.075.3574451
GTATCAA66650.063.0985681
ATCAACG85400.049.093543
TCAACGC87400.047.9019784
CAACGCA88600.047.3445435
TATCAAC91600.045.837022
AACGCAG91850.045.7340936
ACGCAGA111700.037.3903057
CGCAGAG112750.036.88388
TGGTATC14000.034.031782
GCAGAGT122400.033.5870369
GTACATG102650.033.4835741
TACATGG103850.032.6882062
ACATGGG103700.031.815633
GTGGTAT16900.031.370271
GAGTACT64600.029.01035912-13
CATGGGG47150.026.777114
AGTACTT69400.026.57524512-13
CATGGGA65000.026.4785394
GTACTTT71800.025.9769514-15
Produced by FastQC (version 0.11.5)