FastQCFastQC Report
Fri 27 May 2016
SRR940092_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940092_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856159
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG21030.11329848358895979No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA19020.10246966989358132No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37750.058.9684681
GGTATCA21250.058.5399741
ATCAACG45450.047.9292533
TCAACGC45650.047.719274
CAACGCA47450.046.034495
AACGCAG49350.044.2525986
TATCAAC51600.042.6781432
GTACATG105600.038.158381
ACGCAGA59700.036.2757727
TACATGG110500.035.7650832
CGCAGAG60500.035.601338
ACATGGG110550.034.9951673
CATGGGA64950.030.2404484
GCAGAGT70950.029.351399
GTCGTAA1055.895251E-428.3430041
GAGTACA79150.027.2973751
CATGGGG52450.027.121014
TAGACGC1401.0540093E-425.5080154
ATGGGAT30200.025.0293885
AGTACAT82550.024.9466652
Produced by FastQC (version 0.11.5)