FastQCFastQC Report
Fri 27 May 2016
SRR940092_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940092_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856159
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43640.23510916898821704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33590.18096510051132472No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG19900.107210643053747No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19180.10333166501361145No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32250.071.498921
GTATCAA63100.062.5096241
ATCAACG82750.047.209733
TCAACGC84100.046.5227174
CAACGCA85800.045.564085
TATCAAC87650.044.843492
AACGCAG88600.044.122946
GTACATG97700.037.505831
ACGCAGA104200.037.3398557
CGCAGAG106650.036.482078
TACATGG103200.034.9703752
ACATGGG101450.034.633443
GCAGAGT120900.032.1328669
CATGGGA60050.031.0403794
TGGTATC14200.028.0991942
CATGGGG47100.026.9311164
AGAGTAC114950.026.4986410-11
GAGTACT67050.026.17494812-13
CAGAGTA117800.025.55452510-11
AGTACTT71350.025.34791212-13
Produced by FastQC (version 0.11.5)