FastQCFastQC Report
Fri 27 May 2016
SRR940091_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940091_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470867
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG16590.1127906194101846No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG14850.10096086185902602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22600.058.2064631
GGTATCA15200.049.3417241
TCAACGC28350.045.1396334
ATCAACG29100.043.976243
TATCAAC29700.043.6890562
CAACGCA31350.040.820055
AACGCAG31350.040.4265756
GTACATG61100.035.460721
ACGCAGA36950.033.9753077
CGCAGAG37350.033.1346868
TACATGG64900.032.8330272
ACATGGG64950.032.5328253
CATGGGG45450.027.8944844
GCAGAGT44850.026.532479
CGTTTAA1807.2676266E-726.454821
GTAAGGT7650.025.6758654
GTGTAAG6600.025.252331
GAGTACA51300.025.0624641
TAAGGTA5950.025.008964
AGGTAAG6800.023.6334692
Produced by FastQC (version 0.11.5)