##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940091_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1470867 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.135443245378404 34.0 31.0 34.0 30.0 34.0 2 32.14438695000975 34.0 31.0 34.0 30.0 34.0 3 32.310185081315986 34.0 31.0 34.0 30.0 34.0 4 35.63919171481854 37.0 35.0 37.0 35.0 37.0 5 35.6313099688823 37.0 37.0 37.0 35.0 37.0 6 35.65386401353759 37.0 37.0 37.0 35.0 37.0 7 35.560727788440424 37.0 37.0 37.0 35.0 37.0 8 35.504700968884336 37.0 37.0 37.0 35.0 37.0 9 37.21873561647654 39.0 38.0 39.0 35.0 39.0 10-11 37.22641476081794 39.0 38.5 39.0 35.0 39.0 12-13 37.19141737492241 39.0 38.0 39.0 35.0 39.0 14-15 38.714619676694085 41.0 39.5 41.0 35.5 41.0 16-17 38.729989863121546 41.0 39.5 41.0 35.0 41.0 18-19 38.73616411273079 41.0 39.0 41.0 35.0 41.0 20-21 38.78370070169498 41.0 39.0 41.0 35.0 41.0 22-23 38.780542360390164 41.0 39.0 41.0 35.0 41.0 24-25 38.779334229403474 41.0 39.0 41.0 35.0 41.0 26-27 38.76141180677791 41.0 39.0 41.0 35.0 41.0 28-29 38.692346758748414 41.0 39.0 41.0 35.0 41.0 30-31 38.61224944199577 41.0 39.0 41.0 34.0 41.0 32-33 38.538145189197934 41.0 39.0 41.0 34.0 41.0 34-35 38.45024295194603 41.0 39.0 41.0 34.0 41.0 36-37 38.30986044285445 40.0 38.0 41.0 33.5 41.0 38-39 38.16609523498725 40.0 38.0 41.0 33.0 41.0 40-41 38.04597288537985 40.0 38.0 41.0 33.0 41.0 42-43 37.99788526086995 40.0 38.0 41.0 33.0 41.0 44-45 38.04277987064772 40.0 38.0 41.0 33.0 41.0 46-47 37.93601936816857 40.0 38.0 41.0 33.0 41.0 48-49 37.81003041063536 40.0 38.0 41.0 33.0 41.0 50-51 37.6555055623656 40.0 37.0 41.0 33.0 41.0 52-53 37.45927096059671 40.0 37.0 41.0 32.5 41.0 54-55 37.24643220631097 40.0 36.0 41.0 32.0 41.0 56-57 37.029315702915355 40.0 36.0 41.0 32.0 41.0 58-59 36.76750039262558 39.0 35.0 41.0 31.0 41.0 60-61 36.499525789891266 39.0 35.0 41.0 31.0 41.0 62-63 36.20302141526052 38.5 35.0 41.0 31.0 41.0 64-65 35.87913965028789 37.5 35.0 40.0 31.0 41.0 66-67 35.53418833925841 37.0 35.0 40.0 30.0 41.0 68-69 35.19114780602189 36.5 35.0 39.5 30.0 41.0 70-71 35.07777181757426 36.0 35.0 39.0 30.5 41.0 72-73 34.84920118542329 36.0 35.0 39.0 31.0 41.0 74-75 34.52371152524327 35.0 35.0 37.5 30.5 40.0 76-77 34.17495327585703 35.0 35.0 37.0 30.0 39.0 78-79 33.86343258771867 35.0 35.0 37.0 30.0 39.0 80-81 33.57980361242723 35.0 34.0 36.0 30.0 38.0 82-83 33.321773824553816 35.0 34.0 36.0 30.0 37.0 84-85 33.114076935576094 35.0 34.0 35.5 30.0 37.0 86-87 32.932697177922954 35.0 34.0 35.0 29.0 36.0 88-89 32.778391248155 35.0 34.0 35.0 29.0 36.0 90-91 32.66749271008187 35.0 34.0 35.0 29.0 36.0 92-93 32.55100733105033 35.0 34.0 35.0 29.0 36.0 94-95 32.4728897990097 35.0 34.0 35.0 29.0 35.0 96-97 32.36515130191921 35.0 34.0 35.0 29.0 35.0 98-99 32.29664680763115 35.0 34.0 35.0 28.0 35.0 100-101 32.23703468770459 35.0 34.0 35.0 28.0 35.0 102-103 31.45090990551831 34.5 32.5 35.0 26.0 35.0 104-105 32.06227823453786 35.0 33.5 35.0 27.0 35.0 106-107 32.1395027558576 35.0 34.0 35.0 27.0 35.0 108-109 32.092030074779025 35.0 34.0 35.0 27.0 35.0 110-111 32.0213183788881 35.0 34.0 35.0 27.0 35.0 112-113 31.93898428613872 35.0 34.0 35.0 27.0 35.0 114-115 31.82762377563709 35.0 34.0 35.0 26.0 35.0 116-117 31.75492379664511 35.0 34.0 35.0 25.0 35.0 118-119 31.62696899175792 35.0 34.0 35.0 25.0 35.0 120-121 31.497446743995205 35.0 34.0 35.0 25.0 35.0 122-123 31.28419394819518 35.0 33.0 35.0 24.5 35.0 124-125 29.14321349245037 33.5 29.0 34.5 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05896977404030679 1101 2 0.15332618563452627 1101 3 0.13444394084940825 1101 4 0.012374045371508657 1101 5 0.016010444252458456 1101 6 0.05821162911340849 1101 7 0.01460000391651306 1101 8 0.04800787867170442 1101 9 0.01935301611811724 1101 10-11 7.259824528418335E-4 1101 12-13 -0.010295546985929604 1101 14-15 0.11082936659365572 1101 16-17 0.13798505082919377 1101 18-19 0.15953187399857427 1101 20-21 0.058727646521163024 1101 22-23 0.008729888401013852 1101 24-25 0.0871737187945385 1101 26-27 0.1752182407858882 1101 28-29 0.1385092493378508 1101 30-31 0.09322375909986391 1101 32-33 0.12547148435768918 1101 34-35 0.1471038807850107 1101 36-37 0.12691682091275425 1101 38-39 0.11597317187639788 1101 40-41 0.10843114210454985 1101 42-43 0.15532654041135174 1101 44-45 0.058766473389113116 1101 46-47 0.09180074003030114 1101 48-49 0.02301624572251626 1101 50-51 0.021083010758751186 1101 52-53 0.07143637438626627 1101 54-55 0.10146680890851911 1101 56-57 -0.04170863145780146 1101 58-59 0.0847484858426597 1101 60-61 0.059431444325248606 1101 62-63 0.026947139810417298 1101 64-65 0.10360065472914926 1101 66-67 0.09782826139359457 1101 68-69 0.036850458143355524 1101 70-71 0.05728419459795475 1101 72-73 0.08910091426452027 1101 74-75 -0.0036534809139396884 1101 76-77 0.003079116738710752 1101 78-79 0.022430563535770887 1101 80-81 0.04726706235985745 1101 82-83 0.008274202426555632 1101 84-85 0.0674858754972334 1101 86-87 0.06611764444453883 1101 88-89 0.011909000737013287 1101 90-91 0.021512156251198178 1101 92-93 0.027034661884520972 1101 94-95 -0.016471365223992507 1101 96-97 -0.015763640934444823 1101 98-99 0.0011192423695547404 1101 100-101 0.07395239559984645 1101 102-103 -0.04307527890058793 1101 104-105 -0.009498584069767446 1101 106-107 0.0135269701225198 1101 108-109 -0.0010568614682924249 1101 110-111 -0.015646457581450335 1101 112-113 -0.06440119161144509 1101 114-115 -0.10415745365159523 1101 116-117 -0.1406594282811433 1101 118-119 -0.18007226911333518 1101 120-121 -0.12946562649682747 1101 122-123 -0.0952919375172101 1101 124-125 -0.2178600568851543 1102 1 0.07713617988276411 1102 2 0.1533196650294144 1102 3 0.09458348179881426 1102 4 0.10213017474031716 1102 5 0.13970632323002974 1102 6 0.15125414345873622 1102 7 0.18223172013977518 1102 8 0.20855169713805566 1102 9 0.12640179024435838 1102 10-11 0.24043972788842183 1102 12-13 0.1659564091956014 1102 14-15 0.2759408689410705 1102 16-17 0.20273791989544065 1102 18-19 0.25022697050353315 1102 20-21 0.23615005132032252 1102 22-23 0.23652722799413084 1102 24-25 0.16211177304597868 1102 26-27 0.09738503786466168 1102 28-29 0.16806389200869631 1102 30-31 0.17555617954847946 1102 32-33 -0.031244738907830083 1102 34-35 0.046282284540886565 1102 36-37 -0.035899428435186564 1102 38-39 -0.05970497105526107 1102 40-41 0.05247456933459915 1102 42-43 -0.016034961936064462 1102 44-45 -0.05987593662653978 1102 46-47 -0.003970647554467632 1102 48-49 -0.036047395383377534 1102 50-51 -0.021405252152050025 1102 52-53 0.014038747886523595 1102 54-55 0.009571921062928368 1102 56-57 0.06205375769192045 1102 58-59 -0.09186862422515674 1102 60-61 -0.017629066890194167 1102 62-63 0.04335950287770629 1102 64-65 -0.11408974694012386 1102 66-67 0.01593598179005795 1102 68-69 -0.12124487310338594 1102 70-71 -0.007618648385872007 1102 72-73 -0.12700172005994403 1102 74-75 -0.0410100276014731 1102 76-77 -0.06951151967235347 1102 78-79 -0.10046328101299906 1102 80-81 0.0023694358601247245 1102 82-83 -0.09845832235173901 1102 84-85 -0.2147161328491407 1102 86-87 -0.1594952924359987 1102 88-89 -0.08289733729115767 1102 90-91 -0.13981413482861882 1102 92-93 -0.18968090932047943 1102 94-95 -0.06696367090995636 1102 96-97 -0.08185975465379869 1102 98-99 -0.12131489951881491 1102 100-101 -0.12891341034693937 1102 102-103 -0.1380087687284437 1102 104-105 -0.12291472908802703 1102 106-107 -0.18116525731618438 1102 108-109 -0.2596934029393445 1102 110-111 -0.22611854939156828 1102 112-113 -0.14216592817900064 1102 114-115 -0.15561154859160098 1102 116-117 -0.16795468128062296 1102 118-119 -0.19457409488276411 1102 120-121 -0.1637379269663093 1102 122-123 -0.2585939719460022 1102 124-125 -0.27129315547670174 1103 1 -0.18009697578212425 1103 2 -0.18450492154978093 1103 3 -0.13843039000657598 1103 4 -0.1823802120302176 1103 5 -0.3169391598914686 1103 6 -0.13257973654673094 1103 7 -0.4717589864339544 1103 8 -0.1690364537434519 1103 9 -0.15434680890830066 1103 10-11 -0.10461374347962504 1103 12-13 -0.208269708065572 1103 14-15 -0.07960187398636265 1103 16-17 -0.3693809471265581 1103 18-19 -0.1973369218020622 1103 20-21 -0.27170238137067315 1103 22-23 -0.37184198069795826 1103 24-25 -0.2525262275006952 1103 26-27 -0.3164722465415153 1103 28-29 -0.3609088768856594 1103 30-31 -0.277704207222051 1103 32-33 -0.277530785101618 1103 34-35 -0.23429889627092848 1103 36-37 -0.30992139782154027 1103 38-39 -0.31880022867450464 1103 40-41 -0.2926438193492942 1103 42-43 -0.3166499680861321 1103 44-45 -0.39112849915216685 1103 46-47 -0.35968935057483264 1103 48-49 -0.32038694711223314 1103 50-51 -0.3375030381299098 1103 52-53 -0.39257342240184556 1103 54-55 -0.37755736689662456 1103 56-57 -0.4579629841421706 1103 58-59 -0.4606963541691229 1103 60-61 -0.5220126940598036 1103 62-63 -0.4988965446827649 1103 64-65 -0.4765350797267871 1103 66-67 -0.4963402243053423 1103 68-69 -0.5535318418063966 1103 70-71 -0.5965015580483026 1103 72-73 -0.6873859280686929 1103 74-75 -0.5154035632034919 1103 76-77 -0.676952239912886 1103 78-79 -0.5036783964974845 1103 80-81 -0.5784893518944259 1103 82-83 -0.5753605705408944 1103 84-85 -0.574182956684048 1103 86-87 -0.6437579683960948 1103 88-89 -0.531844139259178 1103 90-91 -0.4795435321225838 1103 92-93 -0.44478289784036207 1103 94-95 -0.5346510769506807 1103 96-97 -0.6699329062186479 1103 98-99 -0.59508915809473 1103 100-101 -0.6695320040328809 1103 102-103 -0.7144335329760878 1103 104-105 -0.5598244940190114 1103 106-107 -0.6047746600768775 1103 108-109 -0.5776695202005158 1103 110-111 -0.5188221847612589 1103 112-113 -0.4883545983990345 1103 114-115 -0.431253888348337 1103 116-117 -0.5431562666298113 1103 118-119 -0.5221434539096883 1103 120-121 -0.6281751963390008 1103 122-123 -0.6593422294285745 1103 124-125 -0.5397971530166785 1104 1 0.128662706409294 1104 2 0.09564949485924501 1104 3 0.12935575407108502 1104 4 0.06715770226784201 1104 5 0.08607769460139991 1104 6 0.09150689621149155 1104 7 0.023723211631264007 1104 8 0.04184749293385437 1104 9 0.09803592437849318 1104 10-11 0.19682736552605462 1104 12-13 0.17804349628269023 1104 14-15 0.040524202274404786 1104 16-17 0.0828492812568058 1104 18-19 0.23102026379682172 1104 20-21 0.19012277404304712 1104 22-23 0.21914734811424808 1104 24-25 0.15546137264557558 1104 26-27 0.09555195478157685 1104 28-29 0.17027235046715816 1104 30-31 0.2317436169859164 1104 32-33 0.17501151734843035 1104 34-35 0.1534894917480898 1104 36-37 0.2230657867800261 1104 38-39 0.048453187102900586 1104 40-41 0.19644979455981826 1104 42-43 0.1544605335594298 1104 44-45 0.21178322253261683 1104 46-47 0.050990563656739596 1104 48-49 0.12336201402602853 1104 50-51 0.26395510820830737 1104 52-53 0.14759105018882224 1104 54-55 0.10553037952138311 1104 56-57 0.12512933326749476 1104 58-59 0.11201025465372538 1104 60-61 0.12008364582251829 1104 62-63 0.035664307682516494 1104 64-65 0.07020704985667692 1104 66-67 0.032214760568827217 1104 68-69 0.06141904081052729 1104 70-71 -0.0028826623998909895 1104 72-73 0.045927459119234015 1104 74-75 0.10577425668281393 1104 76-77 -0.17254580395663055 1104 78-79 0.009659341609619787 1104 80-81 -0.004280964540278376 1104 82-83 0.0365954814520677 1104 84-85 0.12011244572943269 1104 86-87 0.16860781066710473 1104 88-89 0.17301482487100373 1104 90-91 0.20399217147769377 1104 92-93 0.16090092251135246 1104 94-95 0.13569523799895933 1104 96-97 0.13117202712798814 1104 98-99 0.22459976514585378 1104 100-101 0.17707382689029316 1104 102-103 0.1271188588991805 1104 104-105 0.13399843407513146 1104 106-107 0.019785693634766233 1104 108-109 0.08251755802161398 1104 110-111 0.13509266181963753 1104 112-113 0.17910534309227444 1104 114-115 0.08506662958657785 1104 116-117 0.06728055395461041 1104 118-119 0.08863411332544047 1104 120-121 0.06937018114179949 1104 122-123 0.13006969171766158 1104 124-125 0.10883071839716862 1105 1 -0.14571295826487685 1105 2 -0.17111435175534595 1105 3 -0.05251499048623032 1105 4 -0.05982959710751601 1105 5 -0.1390350708098751 1105 6 -0.014957098312208927 1105 7 0.020175837934637286 1105 8 0.019600619194442004 1105 9 0.027814549679504808 1105 10-11 0.10688182296857462 1105 12-13 0.004494777957859242 1105 14-15 -0.03153595150216404 1105 16-17 0.05964435991755579 1105 18-19 0.03681988974570061 1105 20-21 0.015366029510182955 1105 22-23 0.005570270358063567 1105 24-25 0.0680822363858411 1105 26-27 0.12226788373632047 1105 28-29 0.15837025162475982 1105 30-31 0.020276077533303294 1105 32-33 0.1925592210901641 1105 34-35 0.16038223931248297 1105 36-37 0.08677916094116966 1105 38-39 0.22225769038501397 1105 40-41 0.16167006910845316 1105 42-43 0.23733455412001803 1105 44-45 0.24934877315786963 1105 46-47 0.1403585272784369 1105 48-49 0.07525091106418103 1105 50-51 0.04277385232555986 1105 52-53 0.12211798553665432 1105 54-55 0.029268262587628158 1105 56-57 0.06701694679689751 1105 58-59 0.1127308531355169 1105 60-61 0.04893081049058168 1105 62-63 0.11673316487078012 1105 64-65 0.07118526687966664 1105 66-67 -0.08842039290214387 1105 68-69 0.022914695216634584 1105 70-71 0.00809225417204118 1105 72-73 0.05639600093105912 1105 74-75 -0.0788743675180541 1105 76-77 -0.05175968540932274 1105 78-79 0.04600953169115485 1105 80-81 0.05215491502992364 1105 82-83 0.0120896323193449 1105 84-85 0.09396615018015808 1105 86-87 0.039738337394645384 1105 88-89 -0.11179546031241472 1105 90-91 0.13326931157572375 1105 92-93 0.12021186167303455 1105 94-95 0.0832929374623248 1105 96-97 0.14795298719252514 1105 98-99 0.10537708249033528 1105 100-101 0.06530771885553577 1105 102-103 -9.459766283370641E-4 1105 104-105 0.01230469148587332 1105 106-107 0.08180147468039678 1105 108-109 0.03220034800290961 1105 110-111 0.08854927311207561 1105 112-113 0.04169381351656298 1105 114-115 -0.053831936550796655 1105 116-117 0.008306688637464532 1105 118-119 0.07285642926417424 1105 120-121 0.07606306805856633 1105 122-123 0.15643693412967963 1105 124-125 0.1926682873093668 1106 1 2.2429501275667008E-4 1106 2 -0.010268016528776513 1106 3 0.01750004424148699 1106 4 0.07990315821455596 1106 5 0.07225630905556812 1106 6 0.12989760569629993 1106 7 0.034271175173380186 1106 8 0.1361587560204427 1106 9 -0.024568535101252564 1106 10-11 -0.06945060762944877 1106 12-13 0.06486706005357235 1106 14-15 0.02308085843529284 1106 16-17 0.04421833829222521 1106 18-19 0.024825708142564906 1106 20-21 -0.0014382263990881938 1106 22-23 0.09736631449170119 1106 24-25 0.05171407514546189 1106 26-27 0.026960783691528434 1106 28-29 -0.022818072238301568 1106 30-31 0.1158826299295228 1106 32-33 0.18190040758940995 1106 34-35 0.25318232956564657 1106 36-37 0.21045033476791275 1106 38-39 0.04070984042438397 1106 40-41 0.2328146939031086 1106 42-43 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2216 80-81 0.6178543286476454 2216 82-83 0.6168072721901723 2216 84-85 0.6118067716342921 2216 86-87 0.5447932065893397 2216 88-89 0.5873146510129388 2216 90-91 0.527364227744485 2216 92-93 0.5454433596853647 2216 94-95 0.5989598183687974 2216 96-97 0.6759009665937654 2216 98-99 0.6917089796234919 2216 100-101 0.6939301569045213 2216 102-103 0.8176198862423121 2216 104-105 0.7584571032706506 2216 106-107 0.6500725594479491 2216 108-109 0.7127846672360931 2216 110-111 0.7265617624992693 2216 112-113 0.7681167228931258 2216 114-115 0.8297442018957284 2216 116-117 0.9081174434757138 2216 118-119 0.8287202520957884 2216 120-121 0.9459111958407078 2216 122-123 0.8493007648961068 2216 124-125 0.6539626924765152 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10725.0 3 2086.0 4 1669.0 5 1718.0 6 2144.0 7 2390.0 8 2288.0 9 2380.0 10 2854.0 11 3291.0 12 3506.0 13 3485.0 14 3384.0 15 3412.0 16 3220.0 17 3444.0 18 2830.0 19 2806.0 20 2962.0 21 2930.0 22 3494.0 23 4191.0 24 5904.0 25 7842.0 26 10438.0 27 10465.0 28 12051.0 29 15728.0 30 18107.0 31 21646.0 32 28967.0 33 40925.0 34 63173.0 35 111029.0 36 262104.0 37 552641.0 38 237815.0 39 823.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.15049822705283 18.012531319800235 13.106006262260191 24.730964190886745 2 15.835915901868288 20.33937602243197 38.077916285344024 25.746791790355722 3 18.366307653500733 24.15285380451743 29.786964973269487 27.693873568712345 4 13.107768929314828 16.254386925279636 36.15533642557306 34.48250771983247 5 14.287258847937267 37.82953453597416 33.42234480282498 14.460861813263593 6 33.24776583837332 35.391214586957204 17.22123470155191 14.139784873117572 7 29.64982587162775 32.43893948274151 20.45787398844402 17.45336065718672 8 27.70214915522039 33.72557631367291 19.949458037049148 18.622816494057545 9 27.487047336714852 15.879217264778275 18.999143288412636 37.63459211009424 10-11 25.462907880736847 25.955305211559182 27.277418389859026 21.304368517844942 12-13 26.125597834419285 23.145374769815263 27.339810630667934 23.38921676509752 14-15 23.039020531055648 24.7282794391281 24.69047365681529 27.54222637300096 16-17 23.84406007824735 28.21837191253892 25.056625478422827 22.8809425307909 18-19 23.87976436704239 26.976696433549414 26.12645689785295 23.01708230155525 20-21 24.25921229670534 26.317297748962666 26.055065394915673 23.368424559416333 22-23 24.208143181296688 26.216318656570547 26.137283396646527 23.438254765486235 24-25 23.971321388444586 26.438987409763172 26.042395799514885 23.54729540227736 26-27 23.782806420448406 26.846993907658295 25.629783331282386 23.74041634061092 28-29 23.570092072717188 26.979169074663073 25.73902259595837 23.71171625666137 30-31 23.677080776717247 26.440095808090113 25.893169764293877 23.989653650898763 32-33 23.47574738994814 26.676987215573018 25.751367234772772 24.095898159706074 34-35 24.20828953827201 26.713752306464357 25.341737452629946 23.736220702633688 36-37 24.35299072978981 26.249391057097682 25.335220305598398 24.062397907514107 38-39 23.833068578116613 26.272465242166888 25.556079438122914 24.33838674159358 40-41 24.014048858232794 26.21010937126566 25.898154277024695 23.877687493476856 42-43 24.05777581208991 26.359772326204617 25.55948730901826 24.022964552687213 44-45 23.656248799592348 26.480287479149023 25.79289793789936 24.070565783359264 46-47 23.883707442313014 26.547096391677925 25.443977483428398 24.125218682580662 48-49 24.110485840997367 26.43920341630004 25.215677395975472 24.234633346727122 50-51 23.873432998189713 26.518047611358053 25.476886988891817 24.131632401560417 52-53 24.322266271694517 26.26543270731109 25.4685308409385 23.9437701800559 54-55 23.58749211755927 26.268796752859352 25.60879637892146 24.534914750659915 56-57 23.70876106062316 26.560929806263168 25.759982267647025 23.970326865466653 58-59 24.25527039413382 25.912510505430188 25.39325666975823 24.438962430677762 60-61 23.67530853915358 26.210726165445998 25.89478479170951 24.21918050369091 62-63 23.603617507988044 26.33581986297854 25.922063052444894 24.138499576588522 64-65 23.587483427949824 26.443077132270453 25.605466227011593 24.363973212768126 66-67 23.584071849366524 26.580288335695908 25.470151714478995 24.365488100458577 68-69 23.634911801988828 26.01833681030875 26.047129362182087 24.299622025520332 70-71 23.485518339063173 26.194436597286458 25.870567106458797 24.449477957191572 72-73 23.608783015939977 26.23214354308682 25.621786959547176 24.537286481426023 74-75 23.433031217502663 26.674070833193102 25.618790129273755 24.27410782003048 76-77 23.757200220439024 26.303638093794017 25.700796524651672 24.23836516111529 78-79 23.808104157459972 26.32861950572798 25.786993915083116 24.07628242172893 80-81 23.943164979227554 26.46274846284569 25.71540948118344 23.878677076743323 82-83 23.648696653666235 26.417064707888 25.91072432000984 24.02351431843592 84-85 23.65604775958669 26.25301947762782 25.688998393464534 24.40193436932095 86-87 23.986902797942818 26.1285237738625 25.63935034777545 24.24522308041923 88-89 23.688015254122625 26.31512925992417 25.606542764881112 24.390312721072085 90-91 23.704827550570844 26.32811352671457 25.783412926772225 24.183645995942367 92-93 23.746828505930946 25.853519895207683 25.979206761865033 24.42044483699634 94-95 23.49236878657282 25.85743646434382 26.069726222697227 24.580468526386138 96-97 23.66278289099083 25.963099881872033 25.80414552685924 24.569971700277897 98-99 23.51800772483226 26.040850098685038 25.779706983963596 24.66143519251911 100-101 23.178710243686208 26.040049848150787 26.163480450645775 24.617759457517234 102-103 23.40758885745618 25.749506923467585 26.11551554287369 24.727388676202537 104-105 23.117333538214258 25.996091851019838 26.061805594745074 24.824769016020827 106-107 23.29921740034959 26.15212660288116 25.889050471592608 24.659605525176648 108-109 23.39201980872506 26.005750349963662 25.774798129266618 24.827431712044664 110-111 23.109747889332922 26.301923633896312 26.084670077818313 24.503658398952453 112-113 23.131006489643426 26.35872872338617 26.188855762660584 24.32140902430982 114-115 23.060767996518997 26.451823447825735 25.813378749558076 24.6740298060972 116-117 22.98403147812933 26.29175058851524 26.116751225896373 24.607466707459057 118-119 22.949565205567 26.651946886409526 25.572813619706146 24.82567428831732 120-121 22.916939327115372 26.76941475538066 25.89525827474179 24.41838764276218 122-123 22.966488472445164 26.60502275188715 25.75596569914207 24.672523076525614 124-125 23.13319174313456 26.695059549871083 25.55430929872028 24.61743940827408 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 772.0 1 742.0 2 896.0 3 880.0 4 546.0 5 355.0 6 266.5 7 242.5 8 260.5 9 262.0 10 219.0 11 173.5 12 166.0 13 180.5 14 181.0 15 171.5 16 200.0 17 249.0 18 297.0 19 403.0 20 506.0 21 615.5 22 838.5 23 1234.0 24 1811.5 25 2716.0 26 3655.0 27 4722.0 28 6391.5 29 8293.0 30 10332.0 31 12892.0 32 17223.5 33 19720.5 34 20116.0 35 21721.0 36 23650.0 37 25591.0 38 28012.0 39 30250.0 40 33534.0 41 39455.0 42 44828.5 43 48628.5 44 52815.5 45 58660.0 46 61049.5 47 60638.5 48 60677.0 49 59708.5 50 58647.0 51 56531.5 52 54707.5 53 56621.0 54 59576.5 55 57996.0 56 53497.5 57 48465.0 58 45226.0 59 41516.0 60 34381.5 61 28105.5 62 24386.5 63 19685.0 64 15402.0 65 12772.0 66 9447.0 67 7886.0 68 5994.5 69 3362.0 70 2324.5 71 1761.5 72 1397.5 73 1108.5 74 868.5 75 649.0 76 396.0 77 232.5 78 148.5 79 97.0 80 60.0 81 40.5 82 26.5 83 26.5 84 24.5 85 16.5 86 12.5 87 9.0 88 7.0 89 9.0 90 8.0 91 6.5 92 5.5 93 7.0 94 7.5 95 5.5 96 4.5 97 5.5 98 5.5 99 4.0 100 12.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010334041079173031 2 0.007410595247564872 3 0.005167020539586516 4 0.020668082158346063 5 0.018356520338004726 6 0.019920223922353278 7 0.007682543697016793 8 6.798711236298047E-4 9 0.00863436327009852 10-11 1.3597422472596093E-4 12-13 0.0025495167136117677 14-15 0.013087519129873739 16-17 0.0012577615787151388 18-19 0.00713864679811295 20-21 0.0023115618203413363 22-23 3.059420056334121E-4 24-25 0.005744910994671851 26-27 2.7194844945192185E-4 28-29 0.004963059202497575 30-31 6.798711236298047E-4 32-33 0.0010198066854447071 34-35 6.798711236298046E-5 36-37 0.005472962545219928 38-39 7.818517921742754E-4 40-41 0.009688163511724718 42-43 0.005336988320493967 44-45 8.49838904537256E-4 46-47 0.0087023503824615 48-49 0.002447536045067297 50-51 0.007104653241931459 52-53 0.0031274071686971014 54-55 0.0023455553765228264 56-57 0.007410595247564872 58-59 0.013529435360233114 60-61 0.0016656842528930216 62-63 0.0051330269834050255 64-65 0.0011557809101706681 66-67 8.158453483557656E-4 68-69 3.399355618149023E-5 70-71 0.003977246073234358 72-73 3.739291179963926E-4 74-75 0.004997052758679064 76-77 0.01125186709607327 78-79 0.0011557809101706681 80-81 0.0032973749496045535 82-83 0.0012577615787151388 84-85 0.0 86-87 0.0012577615787151388 88-89 0.0035013362866934943 90-91 0.004011239629415847 92-93 0.010096086185902601 94-95 0.0 96-97 3.059420056334121E-4 98-99 0.0017676649214374922 100-101 0.0 102-103 0.0 104-105 0.006390788562120164 106-107 0.0 108-109 0.0 110-111 3.3993556181490236E-4 112-113 0.00421520096650479 114-115 0.0018356520338004727 116-117 3.059420056334121E-4 118-119 0.003875265404689887 120-121 0.0013937358034410996 122-123 0.0 124-125 0.07699540475107539 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1470867.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.973292177166286 #Duplication Level Percentage of deduplicated Percentage of total 1 61.062655268944496 20.74499428565434 2 15.142505997201924 10.288815610748674 3 7.459202056866737 7.602409526593602 4 4.420150158471419 6.006682112027894 5 2.766428443480359 4.69923408987908 6 1.8952281658786096 3.863228413307339 7 1.3471255237775737 3.203640231302958 8 1.0128316075833084 2.7527379304557407 9 0.7616419589584079 2.328793632547496 >10 3.7724277920504976 22.24352264427091 >50 0.21401877954512227 5.050618029866623 >100 0.13495572749255516 8.132485194335665 >500 0.007820597596428122 1.8166482383406388 >1k 0.0030079221524723547 1.2661900606689833 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 1659 0.1127906194101846 No Hit CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG 1485 0.10096086185902602 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.399355618149024E-5 0.0 12-13 0.0 0.0 0.0 6.798711236298048E-5 0.0 14-15 0.0 0.0 0.0 6.798711236298048E-5 0.0 16-17 0.0 0.0 0.0 6.798711236298048E-5 0.0 18-19 0.0 0.0 0.0 6.798711236298048E-5 0.0 20-21 0.0 0.0 0.0 6.798711236298048E-5 0.0 22-23 0.0 0.0 0.0 3.739291179963926E-4 0.0 24-25 0.0 0.0 0.0 0.0013937358034410998 0.0 26-27 0.0 0.0 0.0 0.0026854909383377287 0.0 28-29 0.0 0.0 0.0 0.0056429303261273794 0.0 30-31 0.0 0.0 0.0 0.014787196938948255 0.0 32-33 0.0 0.0 0.0 0.029778355214985446 0.0 34-35 0.0 0.0 0.0 0.04966458558115724 0.0 36-37 0.0 0.0 0.0 0.08144856061085061 0.0 38-39 0.0 0.0 0.0 0.13427454691688645 0.0 40-41 0.0 0.0 0.0 0.1963127869481061 0.0 42-43 0.0 0.0 0.0 0.25542758114771763 0.0 44-45 0.0 0.0 0.0 0.32344868706687957 0.0 46-47 0.0 0.0 0.0 0.40649494481826026 0.0 48-49 0.0 0.0 0.0 0.4964758880306649 0.0 50-51 0.0 0.0 0.0 0.597232788552602 0.0 52-53 0.0 0.0 0.0 0.6953721852485643 0.0 54-55 0.0 0.0 0.0 0.8009901643044544 0.0 56-57 0.0 0.0 0.0 0.9243527796870825 0.0 58-59 0.0 0.0 0.0 1.0596811268455952 0.0 60-61 0.0 0.0 0.0 1.207315141341807 0.0 62-63 0.0 0.0 0.0 1.3532154844727633 0.0 64-65 0.0 0.0 0.0 1.5112175336043299 0.0 66-67 0.0 0.0 0.0 1.6859444123771898 0.0 68-69 0.0 0.0 0.0 1.8900757172470386 0.0 70-71 0.0 0.0 0.0 2.117390627432664 0.0 72-73 0.0 0.0 0.0 2.3516742166354945 0.0 74-75 0.0 0.0 0.0 2.5924165815128086 0.0 76-77 0.0 0.0 0.0 2.8585521328576955 0.0 78-79 0.0 0.0 0.0 3.1554518525468316 0.0 80-81 0.0 0.0 0.0 3.4791045009508 0.0 82-83 0.0 0.0 0.0 3.8290341682830604 0.0 84-85 0.0 0.0 0.0 4.187972128003416 0.0 86-87 0.0 0.0 0.0 4.564518749825783 0.0 88-89 0.0 0.0 0.0 4.96683248723372 0.0 90-91 0.0 0.0 0.0 5.429892709537979 0.0 92-93 0.0 0.0 0.0 5.9097457485958955 0.0 94-95 0.0 0.0 0.0 6.402312377665689 0.0 96-97 0.0 0.0 0.0 6.907184674073182 0.0 98-99 0.0 0.0 0.0 7.447036339791429 0.0 100-101 0.0 0.0 0.0 8.056438821457004 0.0 102-103 0.0 0.0 0.0 8.690146695792347 0.0 104-105 0.0 0.0 0.0 9.343400864932043 0.0 106-107 0.0 0.0 0.0 10.019906626499882 0.0 108-109 0.0 0.0 0.0 10.726870614406334 0.0 110-111 0.0 0.0 0.0 11.473029172590042 0.0 112-113 0.0 0.0 0.0 12.254031125859782 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 2260 0.0 58.206463 1 GGTATCA 1520 0.0 49.341724 1 TCAACGC 2835 0.0 45.139633 4 ATCAACG 2910 0.0 43.97624 3 TATCAAC 2970 0.0 43.689056 2 CAACGCA 3135 0.0 40.82005 5 AACGCAG 3135 0.0 40.426575 6 GTACATG 6110 0.0 35.46072 1 ACGCAGA 3695 0.0 33.975307 7 CGCAGAG 3735 0.0 33.134686 8 TACATGG 6490 0.0 32.833027 2 ACATGGG 6495 0.0 32.532825 3 CATGGGG 4545 0.0 27.894484 4 GCAGAGT 4485 0.0 26.53247 9 CGTTTAA 180 7.2676266E-7 26.45482 1 GTAAGGT 765 0.0 25.675865 4 GTGTAAG 660 0.0 25.25233 1 GAGTACA 5130 0.0 25.062464 1 TAAGGTA 595 0.0 25.00896 4 AGGTAAG 680 0.0 23.633469 2 >>END_MODULE