FastQCFastQC Report
Fri 27 May 2016
SRR940091_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940091_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1470867
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37430.2544757615746359No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29530.20076594280788135No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG19030.12937947482675186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18370.12489232541079513No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25450.071.877091
GTATCAA43550.058.422431
TATCAAC60650.041.54762
TCAACGC61250.041.3337174
ATCAACG61250.041.1392063
AACGCAG64450.039.33646
CAACGCA64800.039.1239365
ACGCAGA75650.033.5810247
CGCAGAG76400.033.251378
GTACATG60850.033.1954841
GTGGTAT8200.031.9726541
TACATGG62200.031.7968522
ACATGGG62450.030.813
GCAGAGT83000.029.9621689
CATGGGG44950.027.6973484
GAGTACT54050.026.58253912-13
CAGAGTA79950.026.30546410-11
AGTACTT55300.025.98166812-13
AGAGTAC82650.025.55409610-11
GTAAGGT7850.025.0418194
Produced by FastQC (version 0.11.5)