FastQCFastQC Report
Fri 27 May 2016
SRR940090_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940090_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260067
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44100.059.392751
GGTATCA26750.056.523531
TCAACGC55800.045.9793054
ATCAACG56900.045.195043
CAACGCA61200.042.017715
AACGCAG61250.041.77616
TATCAAC67350.038.7129142
GTACATG136500.037.0249371
TACATGG138800.035.6823272
ACGCAGA73350.034.8784337
ACATGGG140200.034.2220763
CGCAGAG76550.033.1131948
CATGGGA76800.030.9265164
CATGGGG67850.028.6013874
CCGTTGC4400.027.046689
GCAGAGT92600.026.602729
GAGTACA101250.025.3984971
AGTACAT99400.024.733352
TCCGTTG4850.024.5371978
ATGGGAG38200.023.9970265
Produced by FastQC (version 0.11.5)