FastQCFastQC Report
Fri 27 May 2016
SRR940090_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940090_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260067
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38770.17154358698215585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29810.1318987445947399No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37400.067.7000351
GTATCAA71500.064.9087141
TCAACGC93350.049.185664
ATCAACG93800.048.9507753
CAACGCA99050.046.442525
AACGCAG100450.045.793216
TATCAAC102600.045.0444872
ACGCAGA116350.039.424257
CGCAGAG120250.038.0961538
GTACATG132600.034.9997941
TACATGG134100.033.9750372
ACATGGG136050.033.0050853
GCAGAGT142050.031.9145839
GTGGTAT16850.030.0531921
CATGGGA75400.029.3812354
TATTCCG4650.028.1553235
GAGTACT66650.026.86840212-13
CATGGGG63500.026.4468424
CAGAGTA131750.025.9878410-11
AGAGTAC128700.025.95653310-11
Produced by FastQC (version 0.11.5)