FastQCFastQC Report
Fri 27 May 2016
SRR940089_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940089_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819667
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20750.067.1091161
GTATCAA41250.058.4271661
TCAACGC50200.047.2991074
ATCAACG51000.046.6738433
CAACGCA51800.045.838135
TATCAAC52700.045.619922
AACGCAG52650.045.0931476
GTACATG113550.040.0396271
TACATGG120400.037.020232
ACGCAGA63700.036.9865727
TATCACG650.001939825536.6129657
CGCAGAG65350.035.691488
ACATGGG121550.035.2991333
CATGGGA72200.030.5788024
CATGGGG56300.030.4416284
GCAGAGT74000.029.9917099
ATGGGAG30100.028.0740855
TACCGTA850.00721378227.998157
GTATAGA4400.025.6971361
ATGGGAT30600.025.4761415
Produced by FastQC (version 0.11.5)