FastQCFastQC Report
Fri 27 May 2016
SRR940089_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940089_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819667
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32870.18063744630198822No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26200.14398238798637333No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG19550.10743723989059537No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29750.070.679621
GTATCAA60700.062.5111121
ATCAACG79100.047.5777323
TCAACGC79250.047.487684
TATCAAC80100.047.1337282
CAACGCA81500.046.288425
AACGCAG83100.045.397196
ACGCAGA98050.038.225237
GTACATG104000.038.2031331
CGCAGAG101100.036.8366748
TACATGG112750.035.2805182
ACATGGG113100.033.9594153
GCAGAGT110850.033.059959
CATGGGA67400.030.6572134
CATGGGG49800.027.8605524
GAGTACT52050.027.77427512-13
GTGGTAT15100.027.613891
AGAGTAC104650.026.7755810-11
ATGGGAG29750.026.6014565
CAGAGTA108200.026.14450510-11
Produced by FastQC (version 0.11.5)