FastQCFastQC Report
Fri 27 May 2016
SRR940088_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940088_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68730
Sequences flagged as poor quality0
Sequence length125
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.20224065182598575No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA1120.16295649643532664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.1076676851447694No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGA658.913048E-1173.230772
ACTCTAG150.00422862859.50000462-63
TATTGAA300.00421963959.5000047
GAGCGGG150.00422862859.50000470-71
GTAACAA901.5934347E-952.8888851
TATATGG350.007765271251.02
TGGGGAC350.007765271251.06
GGTATCA4900.046.1428571
TCAACAC1056.2354957E-945.3333323
ACAACAC1352.5465852E-1144.0740744
GTACAAA558.472401E-443.2727281
CGCAGAA558.472401E-443.2727282
GAGAACA3500.040.81
ACATGAG600.001299947539.6666683
TCTATGA300.001304497139.66666858-59
ACATGGA1053.2044045E-739.6666643
GTATCAA7200.038.8402751
TAACAAC1252.9060175E-838.0799982
CAACACA2350.037.978725
AGAACAA2200.037.8636362
Produced by FastQC (version 0.11.5)