##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940088_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 68730 Sequences flagged as poor quality 0 Sequence length 125 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.91983122362869 31.0 26.0 34.0 15.0 34.0 2 27.84181580096028 31.0 26.0 34.0 12.0 34.0 3 27.929986905281538 31.0 26.0 34.0 12.0 34.0 4 29.98703622872108 35.0 27.0 37.0 10.0 37.0 5 30.787225374654444 37.0 31.0 37.0 10.0 37.0 6 29.992856103593773 37.0 28.0 37.0 8.0 37.0 7 29.917779717736067 37.0 30.0 37.0 8.0 37.0 8 29.248348610504873 37.0 27.0 37.0 8.0 37.0 9 29.735879528590136 39.0 18.0 39.0 8.0 39.0 10-11 30.338753091808528 39.0 26.0 39.0 7.5 39.0 12-13 30.229746835443038 39.0 24.5 39.0 7.0 39.0 14-15 31.4484068092536 39.5 26.0 41.0 8.0 41.0 16-17 31.817401425869342 39.0 27.0 41.0 8.0 41.0 18-19 32.19491488432999 40.0 28.5 41.0 8.0 41.0 20-21 32.64368543576313 40.0 29.0 41.0 9.5 41.0 22-23 33.096318929143024 40.0 30.0 41.0 14.0 41.0 24-25 33.64445656918376 39.0 30.0 41.0 19.0 41.0 26-27 33.85776953295504 39.0 30.0 41.0 23.0 41.0 28-29 33.79675541975847 39.0 30.0 41.0 23.0 41.0 30-31 33.598297686599736 39.0 30.0 41.0 21.5 41.0 32-33 33.39244871235269 39.0 30.0 41.0 13.0 41.0 34-35 33.07944129201222 39.0 30.0 41.0 2.0 41.0 36-37 32.65330277899025 39.0 30.0 41.0 2.0 41.0 38-39 32.266884911974394 39.0 30.0 41.0 2.0 41.0 40-41 32.0037392696057 38.0 30.0 41.0 2.0 41.0 42-43 31.719103739269606 38.5 29.5 41.0 2.0 41.0 44-45 31.68666521169795 39.0 30.0 41.0 2.0 41.0 46-47 31.4839953441001 39.0 30.0 41.0 2.0 41.0 48-49 31.275098210388478 39.0 30.0 41.0 2.0 41.0 50-51 31.099548959697366 39.0 30.0 41.0 2.0 41.0 52-53 30.874247053688343 38.0 30.0 41.0 2.0 41.0 54-55 30.676684126291285 38.0 30.0 41.0 2.0 41.0 56-57 30.43153644696639 38.0 30.0 41.0 2.0 41.0 58-59 30.16011930743489 38.0 29.5 41.0 2.0 41.0 60-61 29.842681507347592 37.5 29.0 41.0 2.0 41.0 62-63 29.440848246762695 37.0 28.0 41.0 2.0 41.0 64-65 29.08565400843882 36.0 27.0 40.0 2.0 41.0 66-67 28.664949803579223 36.0 26.0 40.0 2.0 41.0 68-69 28.24133566128328 35.0 23.5 40.0 2.0 41.0 70-71 27.98514476938746 35.0 20.0 39.0 2.0 41.0 72-73 27.591837625491053 35.0 5.0 39.0 2.0 41.0 74-75 27.123992434162666 35.0 2.0 38.5 2.0 41.0 76-77 26.639909791939473 35.0 2.0 37.0 2.0 40.5 78-79 26.190680925360105 35.0 2.0 37.0 2.0 39.0 80-81 25.739815218972794 35.0 2.0 36.5 2.0 39.0 82-83 25.28348610504874 35.0 2.0 36.0 2.0 39.0 84-85 24.87536737960134 34.0 2.0 36.0 2.0 37.0 86-87 24.508751636839808 34.0 2.0 35.0 2.0 37.0 88-89 24.181769241961298 34.0 2.0 35.0 2.0 36.5 90-91 23.920784228139098 34.0 2.0 35.0 2.0 36.0 92-93 23.672617488723994 34.0 2.0 35.0 2.0 36.0 94-95 23.43753819292885 34.0 2.0 35.0 2.0 36.0 96-97 23.20986468790921 33.5 2.0 35.0 2.0 35.5 98-99 23.048188563945875 33.0 2.0 35.0 2.0 35.0 100-101 22.929164847955768 33.0 2.0 35.0 2.0 35.0 102-103 22.28844027353412 31.5 2.0 34.5 2.0 35.0 104-105 22.653142732431252 33.0 2.0 35.0 2.0 35.0 106-107 22.63741452058781 33.0 2.0 35.0 2.0 35.0 108-109 22.548937872835733 33.0 2.0 35.0 2.0 35.0 110-111 22.402931761967118 33.0 2.0 35.0 2.0 35.0 112-113 22.254030263349335 32.5 2.0 35.0 2.0 35.0 114-115 22.02773897861196 32.0 2.0 35.0 2.0 35.0 116-117 21.814702458897134 32.0 2.0 35.0 2.0 35.0 118-119 21.534526407682236 31.0 2.0 35.0 2.0 35.0 120-121 21.16764149570784 31.0 2.0 35.0 2.0 35.0 122-123 20.762018041612105 30.5 2.0 35.0 2.0 35.0 124-125 19.065655463407538 27.0 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8822912031430867 1101 2 1.015064929508128 1101 3 0.4519433815673537 1101 4 0.48284204468549063 1101 5 1.617468437441694 1101 6 0.19636463141953087 1101 7 1.3019736472791656 1101 8 0.8579140822361921 1101 9 0.6128831045944132 1101 10-11 1.0132578354769528 1101 12-13 0.8941188940970584 1101 14-15 0.43981373433329196 1101 16-17 1.6396285817293403 1101 18-19 1.5450227661366043 1101 20-21 0.9171701354024719 1101 22-23 1.135834898716439 1101 24-25 1.0618602153645824 1101 26-27 0.8344851475605708 1101 28-29 0.7267263161520532 1101 30-31 0.5837259342644501 1101 32-33 0.0272312536942394 1101 34-35 0.03602973164068857 1101 36-37 -0.2152044419686021 1101 38-39 0.013222463513379523 1101 40-41 0.38773747762624566 1101 42-43 0.6893879500329376 1101 44-45 1.0223698033997124 1101 46-47 1.0827579622517511 1101 48-49 0.9079855235197023 1101 50-51 0.6408177158144461 1101 52-53 0.4938585420122692 1101 54-55 0.32978118812201274 1101 56-57 0.7430750791714225 1101 58-59 0.23367776962954778 1101 60-61 0.4397423604873332 1101 62-63 0.10945018790428307 1101 64-65 0.043711305500867326 1101 66-67 -0.27456324677156374 1101 68-69 0.003608091805119784 1101 70-71 0.0634014173098656 1101 72-73 0.26686164984687366 1101 74-75 0.20214407966572523 1101 76-77 0.18034198323250905 1101 78-79 -0.24705956954913688 1101 80-81 0.21736822652302124 1101 82-83 0.14213239313240322 1101 84-85 0.15753083866096063 1101 86-87 0.1123851247039056 1101 88-89 0.23842675302463334 1101 90-91 0.534742674241798 1101 92-93 0.5726828273372071 1101 94-95 0.8421552020965599 1101 96-97 1.1379250024607117 1101 98-99 0.3074336090148577 1101 100-101 0.24906668864340986 1101 102-103 0.12133797596931117 1101 104-105 0.22380734035104055 1101 106-107 0.17757236001137144 1101 108-109 -0.19085068031220231 1101 110-111 0.4719215282028699 1101 112-113 -0.15475027482862203 1101 114-115 -0.8344242684852397 1101 116-117 -0.31585157875877456 1101 118-119 -0.45262005010748396 1101 120-121 -0.1251473960671241 1101 122-123 -0.9276594640143649 1101 124-125 -0.45963426863917434 1102 1 0.6345429665722762 1102 2 0.7593948264153454 1102 3 -0.06786128473758168 1102 4 -0.18367993035520414 1102 5 -0.6093356862696453 1102 6 0.6700488419458459 1102 7 -0.22130361804910237 1102 8 0.7315982927625093 1102 9 0.7413692684576816 1102 10-11 0.3531384648855287 1102 12-13 0.9866853618127394 1102 14-15 0.8432863333566232 1102 16-17 -0.014142443772556135 1102 18-19 -0.012546414547067286 1102 20-21 0.4396877696943875 1102 22-23 -0.3478330340019582 1102 24-25 -0.06402149923118827 1102 26-27 0.19259692184162702 1102 28-29 0.7655760177255715 1102 30-31 1.2211106331250008 1102 32-33 0.9857228434934768 1102 34-35 1.4242011912174632 1102 36-37 1.4125763502180533 1102 38-39 1.5595599567309577 1102 40-41 1.7134564141427902 1102 42-43 1.4197189321273456 1102 44-45 1.2688700746965083 1102 46-47 0.7735881304557672 1102 48-49 0.6799334345343517 1102 50-51 0.6776598210776044 1102 52-53 0.600640961979714 1102 54-55 0.7655923655501162 1102 56-57 1.2557175099907418 1102 58-59 0.6673185726680728 1102 60-61 0.5225421434498969 1102 62-63 0.1721197484034711 1102 64-65 0.07192617256543343 1102 66-67 0.5320021357569225 1102 68-69 0.6812532355924219 1102 70-71 0.821567450950667 1102 72-73 0.6161834078501229 1102 74-75 0.16595308671944053 1102 76-77 0.329121147638368 1102 78-79 0.58251178150892 1102 80-81 0.48871928458054015 1102 82-83 0.4710526318735866 1102 84-85 0.45682546698434834 1102 86-87 0.39773509757422687 1102 88-89 0.17700515779945647 1102 90-91 0.1843899884034883 1102 92-93 -0.11282992361233468 1102 94-95 -0.15806183534239793 1102 96-97 -0.17097353253657133 1102 98-99 0.0039067506317849165 1102 100-101 -0.2944493178677092 1102 102-103 -0.7115974553925994 1102 104-105 -0.526870901645708 1102 106-107 -0.16312215979329636 1102 108-109 -0.1666510058683599 1102 110-111 -0.5635098337070588 1102 112-113 -0.2807947675036111 1102 114-115 -0.23285074705279385 1102 116-117 0.1728082150556567 1102 118-119 0.3660451696429199 1102 120-121 -0.23388315298519302 1102 122-123 -0.26759435278267674 1102 124-125 -0.44710035653933744 1103 1 -1.2767508930768443 1103 2 -1.4760218402513239 1103 3 -0.992751635614777 1103 4 -1.3052808075481863 1103 5 -0.6301690196029774 1103 6 -1.5536353685804691 1103 7 -0.9361062496280503 1103 8 -0.8087525844304757 1103 9 -1.396459678910741 1103 10-11 -1.0269053947635989 1103 12-13 -1.8934133223977874 1103 14-15 -2.844926385941619 1103 16-17 -2.1031227069304492 1103 18-19 -2.3037744847225063 1103 20-21 -2.251375826796835 1103 22-23 -1.9993132971598513 1103 24-25 -2.698341674669784 1103 26-27 -2.8112407974566125 1103 28-29 -2.540509508590219 1103 30-31 -2.5722555949451724 1103 32-33 -2.5792442617696807 1103 34-35 -2.282322931589551 1103 36-37 -2.6493754041679125 1103 38-39 -2.1930167976550017 1103 40-41 -2.167245340243177 1103 42-43 -1.9596670327849353 1103 44-45 -1.7593097498648902 1103 46-47 -1.8202166941056355 1103 48-49 -1.8220402496761743 1103 50-51 -1.6873072841855539 1103 52-53 -1.698098073108003 1103 54-55 -1.8399997397130399 1103 56-57 -1.576190384746102 1103 58-59 -1.364260374700347 1103 60-61 -1.5088723302343148 1103 62-63 -1.747562269140392 1103 64-65 -1.223578213399481 1103 66-67 -0.6645439168746563 1103 68-69 -1.0439112380917877 1103 70-71 -0.9807351806282796 1103 72-73 -1.180088521974433 1103 74-75 -0.8704504220524889 1103 76-77 -0.871043326045843 1103 78-79 -1.110032078140204 1103 80-81 -1.286390364542271 1103 82-83 -1.221162280407114 1103 84-85 -1.392297340033199 1103 86-87 -1.6604885866362977 1103 88-89 -1.6513391404461544 1103 90-91 -1.56829641510528 1103 92-93 -1.2925448358930325 1103 94-95 -1.5225355195529247 1103 96-97 -1.4810612518348165 1103 98-99 -1.4104572844559335 1103 100-101 -1.3141313354115738 1103 102-103 -1.1788123676733022 1103 104-105 -0.9418928314702697 1103 106-107 -0.9519379492669806 1103 108-109 -0.7726268830613421 1103 110-111 -0.8607137810754821 1103 112-113 -1.4593035394334315 1103 114-115 -1.398749869859813 1103 116-117 -1.4438584516110105 1103 118-119 -1.4433846549184786 1103 120-121 -1.1476989424588773 1103 122-123 -1.3172105808528514 1103 124-125 -1.450828426714775 1104 1 0.440655020901648 1104 2 0.589502353297064 1104 3 0.0028800848153309744 1104 4 -0.2552136032471104 1104 5 0.5304492599669146 1104 6 0.9598054916345831 1104 7 0.8673211697267895 1104 8 1.051462469332968 1104 9 1.2194677404440988 1104 10-11 1.245272024591241 1104 12-13 1.0402224302903846 1104 14-15 1.2157254957788055 1104 16-17 1.4383759489835342 1104 18-19 1.4489136873205055 1104 20-21 0.8502706785795056 1104 22-23 1.3735025630552045 1104 24-25 0.6057577876958078 1104 26-27 1.0553586649089794 1104 28-29 0.607171943022685 1104 30-31 0.40203875989353577 1104 32-33 -0.15972141230731296 1104 34-35 -0.16555545847127462 1104 36-37 -0.13156626438296115 1104 38-39 0.08638508406542655 1104 40-41 0.5263030468535987 1104 42-43 0.3696340424838809 1104 44-45 0.12274670174801017 1104 46-47 0.35972282202339656 1104 48-49 -0.5095402496761743 1104 50-51 -0.22477368203501769 1104 52-53 -0.11056447095746691 1104 54-55 0.22552445383534447 1104 56-57 0.29040907761948986 1104 58-59 0.43143855003083686 1104 60-61 0.39922578804525344 1104 62-63 0.24286111795638377 1104 64-65 0.3809244747725664 1104 66-67 0.2603552766737316 1104 68-69 0.5278293533060605 1104 70-71 0.22524600216741675 1104 72-73 0.08268029522986353 1104 74-75 0.34325925536686697 1104 76-77 0.199353179330501 1104 78-79 -0.3586879921186963 1104 80-81 -0.6207317623917312 1104 82-83 -0.25039615137485427 1104 84-85 -0.19337260885039953 1104 86-87 -0.19711493072232145 1104 88-89 -0.241896936145082 1104 90-91 -0.31006388822356 1104 92-93 -0.5213754810543243 1104 94-95 -0.01850326148840864 1104 96-97 -0.1867064131251439 1104 98-99 -0.12450298338066546 1104 100-101 -0.38284773326103405 1104 102-103 -0.2524010773507186 1104 104-105 0.19553996422865438 1104 106-107 0.3148631260018355 1104 108-109 0.27256800941177417 1104 110-111 -0.40154039397870633 1104 112-113 -0.3907551523366628 1104 114-115 -0.48712352577378937 1104 116-117 -0.8121380215034826 1104 118-119 -0.4897556226604145 1104 120-121 0.12044621883144302 1104 122-123 0.775699365383705 1104 124-125 0.16274684210242896 1105 1 0.5620971461388393 1105 2 0.8456693362192667 1105 3 0.7309003251695394 1105 4 1.2798907610792654 1105 5 0.2730172549068257 1105 6 0.6571489451450212 1105 7 -0.146277818255502 1105 8 0.6814434940008987 1105 9 0.019594242657888117 1105 10-11 1.0110848013148335 1105 12-13 1.3599567756414253 1105 14-15 1.1131005748426972 1105 16-17 0.6741444499322995 1105 18-19 0.4266176721402317 1105 20-21 0.24464133006591027 1105 22-23 0.70319895774211 1105 24-25 0.37457499199688726 1105 26-27 0.9957444966610325 1105 28-29 0.8960713737627231 1105 30-31 0.7540311697607152 1105 32-33 0.6263317186224739 1105 34-35 0.31357167625358784 1105 36-37 0.26912949779287487 1105 38-39 0.41513271214891745 1105 40-41 0.5435389734823133 1105 42-43 0.6163133593719365 1105 44-45 0.8332147599390254 1105 46-47 1.1849916392277002 1105 48-49 1.039479358166961 1105 50-51 1.2927785001281755 1105 52-53 1.5197328092449318 1105 54-55 1.849338495581076 1105 56-57 1.8092262819205622 1105 58-59 2.091621978240827 1105 60-61 1.6538114932950982 1105 62-63 1.2748029269380403 1105 64-65 1.134877668953461 1105 66-67 1.506718338027305 1105 68-69 0.9380127815160577 1105 70-71 1.222083446822701 1105 72-73 1.4534899093981153 1105 74-75 1.671216244614179 1105 76-77 1.583919909248273 1105 78-79 1.3728110591146994 1105 80-81 1.024271400163613 1105 82-83 1.185087719592886 1105 84-85 1.122408542319743 1105 86-87 0.8033594525793895 1105 88-89 1.253991741906784 1105 90-91 1.1372795652868746 1105 92-93 1.448263987636377 1105 94-95 1.1171703628000174 1105 96-97 1.374330905027925 1105 98-99 1.5631946763283793 1105 100-101 1.5072534685099974 1105 102-103 1.4896925342874852 1105 104-105 0.9496512861767918 1105 106-107 1.311542442889884 1105 108-109 1.4096647836053222 1105 110-111 1.3951072973558922 1105 112-113 1.2539317546842135 1105 114-115 1.1349511105323487 1105 116-117 1.3306513523105608 1105 118-119 1.4548996588070082 1105 120-121 1.6721539987175937 1105 122-123 1.8614046152255774 1105 124-125 2.0381421615205184 1106 1 0.24815852721301113 1106 2 -0.32973560836726534 1106 3 0.20788242235623855 1106 4 0.2187228156402199 1106 5 0.06457735720861635 1106 6 1.2724515879412692 1106 7 1.701122011241516 1106 8 0.6187836474535828 1106 9 -0.1315140267368271 1106 10-11 0.37436272117843217 1106 12-13 0.4265595036891696 1106 14-15 -0.1762216757966968 1106 16-17 0.3948392495912927 1106 18-19 0.703471892089091 1106 20-21 0.26062598479737176 1106 22-23 0.6807138767531953 1106 24-25 -0.04571486307557393 1106 26-27 0.3622193474709263 1106 28-29 0.06614810010543692 1106 30-31 0.5905614340403318 1106 32-33 0.8032285643172727 1106 34-35 0.8478854017437811 1106 36-37 1.1065122769915163 1106 38-39 0.8393654487218143 1106 40-41 1.530218427872768 1106 42-43 1.1127967353310098 1106 44-45 1.2864330037582903 1106 46-47 1.1759336682132009 1106 48-49 1.6861119242368723 1106 50-51 1.3908177158144461 1106 52-53 1.2097370718195322 1106 54-55 1.3080980863739171 1106 56-57 1.7784291804712886 1106 58-59 1.456391799212696 1106 60-61 1.2458831045482945 1106 62-63 1.333733020714682 1106 64-65 0.95893990254255 1106 66-67 0.9135810831253437 1106 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120-121 -0.681352788612724 2216 122-123 -0.583636862904136 2216 124-125 -0.9732643241506764 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3597.0 3 428.0 4 340.0 5 321.0 6 1029.0 7 1712.0 8 1466.0 9 1183.0 10 1185.0 11 1136.0 12 1182.0 13 1180.0 14 1191.0 15 1148.0 16 992.0 17 896.0 18 818.0 19 578.0 20 456.0 21 319.0 22 334.0 23 365.0 24 475.0 25 862.0 26 1302.0 27 844.0 28 594.0 29 842.0 30 933.0 31 918.0 32 1196.0 33 1597.0 34 2314.0 35 3475.0 36 5818.0 37 14246.0 38 11242.0 39 216.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.510172599470266 21.743749454259685 14.446837616788427 17.29924032948162 2 15.257566938300348 24.780267753201397 39.91705471478463 20.04511059371362 3 19.694125522038387 34.69827272594985 28.270834242807872 17.33676750920388 4 11.155587254916231 22.65323648855184 36.58316472831545 29.608011528216476 5 10.564005530892947 47.22654828615094 29.725638599810782 12.483807583145332 6 23.49636916629073 39.8562218956008 21.226188570513845 15.421220367594627 7 24.63548790780245 41.427781496464014 19.571607345537092 14.365123250196444 8 24.57442164993453 39.64498763276589 18.776371308016877 17.0042194092827 9 27.51520619306772 23.260382410290738 18.196501848025378 31.02790954861616 10-11 21.67612396333479 36.14869780299724 23.466462971046123 18.70871526262185 12-13 23.135066711286356 34.95394957005048 24.36162318671885 17.549360531944302 14-15 21.941708016239104 34.03154693479621 24.26552973531423 19.761215313650453 16-17 19.020355308530608 36.28817529718169 25.25644196772833 19.435027426559383 18-19 18.983906641056983 34.85754612653513 28.219399336476343 17.939147895931555 20-21 23.802525899196834 31.993510650680946 26.89296938656734 17.310994063554883 22-23 22.82554925069111 33.001600465589995 25.70856976575004 18.464280517968863 24-25 20.318797561365983 34.3496733452646 26.96538478327295 18.36614431009647 26-27 19.861777971773606 33.54284882875018 27.40142586934381 19.193947330132403 28-29 19.10717273792115 34.98046576598206 26.965245796683906 18.94711569941288 30-31 19.794265926567196 35.272335751023945 26.68795786379939 18.245440458609476 32-33 19.172262274569142 35.04463149011705 27.22339024727373 18.55971598804007 34-35 19.029535864978904 35.79659537319947 26.852902662592754 18.320966099228865 36-37 18.453983266642414 35.88432157148054 26.58857766460531 19.073117497271735 38-39 18.21720089045381 36.30636267077944 26.755809047127123 18.720627391639628 40-41 18.35074648584151 36.168912429789586 27.282384098250922 18.197956986117983 42-43 17.60139682077771 36.40900658397294 27.371867156523955 18.6177294387254 44-45 17.236286919831223 36.47897570202241 27.159173577768076 19.12556380037829 46-47 17.2791362614497 36.78237018821526 26.182074805928018 19.756418744407018 48-49 16.544323596813506 36.99465279546033 26.49157906223855 19.96944454548761 50-51 16.661695053366024 36.294717236462056 26.40726971123415 20.636317998937773 52-53 16.81907796620034 36.941007005827274 25.81680089046029 20.423114137512094 54-55 17.05975643469278 36.275807883135215 25.676934045308386 20.987501636863623 56-57 17.337465805249984 35.86083464291951 25.536202782143064 21.265496769687445 58-59 17.223867348646348 35.59075398528845 25.162430971384502 22.022947694680703 60-61 17.776338954444267 35.29005223413697 24.496937246286137 22.43667156513262 62-63 17.687757819150097 34.92590086649061 24.57821332693105 22.808127987428247 64-65 18.081159335942615 34.74952349081174 24.695543365973606 22.47377380727204 66-67 18.24993816292977 34.60111452225407 24.541314437864656 22.607632876951506 68-69 18.227120616906735 35.27717154081188 24.23541393860032 22.260293903681074 70-71 18.654783557657332 35.54892688250273 24.105492906511458 21.690796653328484 72-73 18.93641786701586 34.803579223046704 24.713371162520005 21.54663174741743 74-75 19.07748272098945 35.01636958894143 24.8526736995271 21.053473990542017 76-77 18.597578720679824 34.91793259996508 25.616960596007214 20.86752808334788 78-79 18.43604274729192 34.78346270524302 25.68474963443645 21.095744913028614 80-81 19.397034644317372 34.50536179376373 25.88284080492383 20.214762756995068 82-83 19.492499527128288 34.372681109866285 25.527070086862896 20.60774927614253 84-85 19.800669285610358 33.5421213443911 25.372471991852173 21.28473737814637 86-87 20.1465174817035 32.892228899009154 25.871902690276304 21.089350929011044 88-89 20.300314282388545 32.53622977534629 25.931934582702826 21.231521359562333 90-91 21.05519704917317 32.27357715000764 25.229714884360472 21.441510916458718 92-93 21.36153308887725 31.95099237529829 25.256096851172806 21.43137768465165 94-95 21.012658227848103 32.19118288956788 25.18114360541248 21.61501527717154 96-97 20.341338144464896 32.6497355575117 24.79139234244393 22.217533955579484 98-99 19.521599324884694 33.86707212384874 24.382720540092244 22.228608011174323 100-101 18.715990106212715 35.586352393423546 24.164120471409863 21.533537028953877 102-103 18.198021242543287 37.71642659682817 23.54284882875018 20.542703331878364 104-105 17.209292173824473 39.25455987311658 23.209335826379238 20.326812126679712 106-107 16.329113924050635 41.497890295358644 22.345409573694166 19.82758620689655 108-109 15.867161356030845 42.63785828604685 22.098792375963917 19.396187981958388 110-111 15.7529463116543 43.71089771569912 21.55245162229012 18.98370435035647 112-113 16.005092761004004 44.59876318661332 21.03164787195344 18.364496180429246 114-115 15.900129494100016 44.74457652519315 20.975861717761063 18.379432262945773 116-117 15.675832969591152 44.81158155099666 21.05266986759785 18.459915611814345 118-119 15.798625004546944 44.81903168309629 20.827907315121312 18.55443599723546 120-121 15.992521351976604 44.341544326266934 20.844912627857237 18.82102169389923 122-123 16.652844463844026 43.903681070856976 20.490324458024155 18.953150007274843 124-125 16.951238159034812 44.26864519699143 19.9066556964053 18.87346094756846 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 97.0 1 70.5 2 54.0 3 48.0 4 24.0 5 16.0 6 11.0 7 14.5 8 21.5 9 24.0 10 25.0 11 26.0 12 25.0 13 23.5 14 36.5 15 60.5 16 81.0 17 98.0 18 127.5 19 177.5 20 219.5 21 282.0 22 399.5 23 532.5 24 641.0 25 753.5 26 921.5 27 1149.5 28 1379.0 29 1678.0 30 1889.5 31 1986.5 32 2200.5 33 2381.5 34 2661.0 35 2927.0 36 2980.0 37 3161.5 38 3133.5 39 2747.0 40 2459.5 41 2304.0 42 2176.5 43 2079.5 44 2079.5 45 2057.0 46 1910.0 47 1782.0 48 1863.0 49 1952.0 50 1949.0 51 1821.5 52 1657.5 53 1527.0 54 1242.0 55 905.0 56 695.5 57 541.5 58 422.5 59 357.5 60 264.0 61 211.5 62 174.0 63 156.0 64 146.0 65 118.0 66 89.5 67 81.5 68 80.0 69 90.5 70 98.5 71 71.0 72 50.5 73 39.0 74 27.5 75 25.5 76 27.5 77 20.5 78 19.5 79 22.5 80 14.0 81 7.5 82 7.5 83 5.5 84 2.0 85 2.5 86 2.0 87 2.0 88 3.5 89 3.0 90 3.5 91 4.0 92 2.0 93 1.5 94 1.0 95 1.0 96 3.0 97 3.0 98 4.0 99 6.0 100 11.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023279499490760948 2 0.014549687181725592 3 0.013094718463553033 4 0.04219409282700422 5 0.03637421795431398 6 0.018914593336243273 7 0.011639749745380474 8 0.0 9 0.011639749745380474 10-11 0.0 12-13 0.0014549687181725592 14-15 0.010184781027207915 16-17 0.0014549687181725592 18-19 0.008729812309035356 20-21 0.0029099374363451185 22-23 0.0 24-25 0.004364906154517678 26-27 0.0 28-29 0.006547359231776516 30-31 7.274843590862796E-4 32-33 7.274843590862796E-4 34-35 0.0 36-37 0.007274843590862796 38-39 0.0014549687181725592 40-41 0.011639749745380474 42-43 0.003637421795431398 44-45 0.0 46-47 0.008002327949949076 48-49 0.003637421795431398 50-51 0.009457296668121637 52-53 0.002182453077258839 54-55 0.0014549687181725592 56-57 0.008729812309035356 58-59 0.012367234104466754 60-61 0.0014549687181725592 62-63 0.006547359231776516 64-65 0.0014549687181725592 66-67 0.0014549687181725592 68-69 0.0 70-71 0.007274843590862796 72-73 0.0 74-75 0.007274843590862796 76-77 0.008729812309035356 78-79 7.274843590862796E-4 80-81 0.004364906154517678 82-83 0.0014549687181725592 84-85 0.0 86-87 0.0014549687181725592 88-89 0.0029099374363451185 90-91 0.0050923905136039576 92-93 0.008729812309035356 94-95 0.0 96-97 7.274843590862796E-4 98-99 0.0014549687181725592 100-101 0.0 102-103 0.0 104-105 0.008002327949949076 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.007274843590862796 114-115 0.0014549687181725592 116-117 0.0 118-119 0.003637421795431398 120-121 0.0014549687181725592 122-123 0.0 124-125 0.08657063873126727 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 68730.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.83602502546195 #Duplication Level Percentage of deduplicated Percentage of total 1 82.98492950899367 57.123526844172844 2 8.670288093678003 11.936563363887677 3 3.438946545200905 7.101702313400262 4 1.6782566422185115 4.620980648916048 5 0.9765170890490584 3.360977738978612 6 0.5960558855234512 2.4618070711479705 7 0.426962017289848 2.057325767495999 8 0.2916869227029655 1.6062854648625053 9 0.1754348882923633 1.0868616324749019 >10 0.748240366933694 7.917939764295068 >50 0.008454693411680158 0.3608322421067947 >100 0.004227346705840079 0.3651971482613124 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 139 0.20224065182598575 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 112 0.16295649643532664 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 74 0.1076676851447694 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0014549687181725592 0.0 18-19 0.0 0.0 0.0 0.0014549687181725592 0.0 20-21 0.0 0.0 0.0 0.006547359231776517 0.0 22-23 0.0 0.0 0.0 0.012367234104466754 0.0 24-25 0.0 0.0 0.0 0.01964207769532955 0.0 26-27 0.0 0.0 0.0 0.03637421795431398 0.0 28-29 0.0 0.0 0.0 0.085843154372181 0.0 30-31 0.0 0.0 0.0 0.2255201513167467 0.0 32-33 0.0 0.0 0.0 0.4350356467335952 0.0 34-35 0.0 0.0 0.0 0.7100247344682089 0.0 36-37 0.0 0.0 0.0 1.0839516950385568 0.0 38-39 0.0 0.0 0.0 1.6564818856394588 0.0 40-41 0.0 0.0 0.0 2.4283427906300012 0.0 42-43 0.0 0.0 0.0 2.9797759348174013 0.0 44-45 0.0 0.0 0.0 3.4679179397642947 0.0 46-47 0.0 0.0 0.0 4.004801396769969 0.0 48-49 0.0 0.0 0.0 4.599883602502546 0.0 50-51 0.0 0.0 0.0 5.309180852611669 0.0 52-53 0.0 0.0 0.0 5.896988214753383 0.0 54-55 0.0 0.0 0.0 6.403317328677433 0.0 56-57 0.0 0.0 0.0 6.980939909791939 0.0 58-59 0.0 0.0 0.0 7.679324894514768 0.0 60-61 0.0 0.0 0.0 8.45118579950531 0.0 62-63 0.0 0.0 0.0 9.195402298850574 0.0 64-65 0.0 0.0 0.0 9.879237596391679 0.0 66-67 0.0 0.0 0.0 10.581260002909938 0.0 68-69 0.0 0.0 0.0 11.343663611232358 0.0 70-71 0.0 0.0 0.0 12.286483340608177 0.0 72-73 0.0 0.0 0.0 13.240942819729376 0.0 74-75 0.0 0.0 0.0 14.0986468790921 0.0 76-77 0.0 0.0 0.0 14.896697221009749 0.0 78-79 0.0 0.0 0.0 15.86206896551724 0.0 80-81 0.0 0.0 0.0 16.84999272515641 0.0 82-83 0.0 0.0 0.0 17.873563218390807 0.0 84-85 0.0 0.0 0.0 18.887676414957077 0.0 86-87 0.0 0.0 0.0 19.85086570638731 0.0 88-89 0.0 0.0 0.0 20.773315873708718 0.0 90-91 0.0 0.0 0.0 21.805616179252148 0.0 92-93 0.0 0.0 0.0 22.93103448275862 0.0 94-95 0.0 0.0 0.0 24.014986177797176 0.0 96-97 0.0 0.0 0.0 24.99199767205005 0.0 98-99 0.0 0.0 0.0 25.99374363451186 0.0 100-101 0.0 0.0 0.0 27.071875454677723 0.0 102-103 0.0 0.0 0.0 28.15800960279354 0.0 104-105 0.0 0.0 0.0 29.303797468354432 0.0 106-107 0.0 0.0 0.0 30.327367961588827 0.0 108-109 0.0 0.0 0.0 31.33566128328241 0.0 110-111 0.0 0.0 0.0 32.408700712934674 0.0 112-113 0.0 0.0 0.0 33.48756001745963 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATGA 65 8.913048E-11 73.23077 2 ACTCTAG 15 0.004228628 59.500004 62-63 TATTGAA 30 0.004219639 59.500004 7 GAGCGGG 15 0.004228628 59.500004 70-71 GTAACAA 90 1.5934347E-9 52.888885 1 TATATGG 35 0.0077652712 51.0 2 TGGGGAC 35 0.0077652712 51.0 6 GGTATCA 490 0.0 46.142857 1 TCAACAC 105 6.2354957E-9 45.333332 3 ACAACAC 135 2.5465852E-11 44.074074 4 GTACAAA 55 8.472401E-4 43.272728 1 CGCAGAA 55 8.472401E-4 43.272728 2 GAGAACA 350 0.0 40.8 1 ACATGAG 60 0.0012999475 39.666668 3 TCTATGA 30 0.0013044971 39.666668 58-59 ACATGGA 105 3.2044045E-7 39.666664 3 GTATCAA 720 0.0 38.840275 1 TAACAAC 125 2.9060175E-8 38.079998 2 CAACACA 235 0.0 37.97872 5 AGAACAA 220 0.0 37.863636 2 >>END_MODULE