SRR940088_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR940088_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	68730
Sequences flagged as poor quality	0
Sequence length	125
%GC	38

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1199	1.7445074930888986	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1060	1.542266841262913	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT	980	1.4258693438091081	No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	553	0.8045977011494253	RNA PCR Primer, Index 46 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	516	0.7507638585770405	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	412	0.5994471118870944	No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT	397	0.577622581114506	RNA PCR Primer, Index 46 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	379	0.5514331441874	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	375	0.5456132693147098	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	348	0.5063291139240507	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	346	0.5034191764877055	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	246	0.3579223046704496	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	215	0.31281827440710025	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	149	0.21679033900771136	No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT	147	0.21388040157136623	RNA PCR Primer, Index 46 (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	141	0.20515058926233085	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	140	0.2036956205441583	No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG	131	0.19060090208060526	RNA PCR Primer, Index 46 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG	128	0.18623599592608758	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	123	0.1789611523352248	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	118	0.171686308744362	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	117	0.17023134002618945	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	109	0.15859159028080896	No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA	103	0.1498617779717736	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	96	0.1396769969445657	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG	95	0.13822202822639315	No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG	93	0.13531209079004802	TruSeq Adapter, Index 4 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	89	0.12949221591735777	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC	80	0.11639749745380473	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	80	0.11639749745380473	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	79	0.11494252873563218	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT	74	0.1076676851447694	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	73	0.10621271642659681	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	70	0.10184781027207915	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGA	69	0.10039284155390658	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGATGT	15	2.6907783E-4	119.00001	1
GGTATCA	830	0.0	74.554214	1
GTATAAG	25	0.0020486002	71.4	8
TTTACAG	30	0.004219639	59.500004	3
GACTTTC	30	0.004219639	59.500004	6
AGATGTG	30	0.004219639	59.500004	2
GCATACG	30	0.004219639	59.500004	7
GTATCAA	1550	0.0	55.66129	1
TATCAAC	2075	0.0	41.578312	2
ATCAACG	2135	0.0	40.40984	3
TCAACGC	2140	0.0	40.315422	4
CAACGCA	2150	0.0	40.127907	5
AACGCAG	2185	0.0	39.485126	6
ATGTGTA	65	0.0019262546	36.615383	4
ACGCAGA	2375	0.0	36.326317	7
CGCAGAG	2365	0.0	36.22833	8
GCAGAGT	2430	0.0	35.014404	9
CATGGGT	105	1.4635492E-5	34.0	4
AGAGTAA	35	0.00278049	34.0	10-11
GATTGGC	35	0.00278049	34.0	16-17

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content