##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940088_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 68730 Sequences flagged as poor quality 0 Sequence length 125 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.44426014840681 33.0 31.0 34.0 31.0 34.0 2 32.498792375963916 33.0 31.0 34.0 31.0 34.0 3 32.4962607303943 34.0 31.0 34.0 30.0 34.0 4 36.04878510112032 37.0 35.0 37.0 35.0 37.0 5 36.11276007565837 37.0 35.0 37.0 35.0 37.0 6 36.240419030990836 37.0 37.0 37.0 35.0 37.0 7 36.17367961588826 37.0 37.0 37.0 35.0 37.0 8 36.213894951258546 37.0 37.0 37.0 35.0 37.0 9 37.93599592608759 39.0 39.0 39.0 35.0 39.0 10-11 37.92423977884475 39.0 38.5 39.0 35.0 39.0 12-13 37.9245234977448 39.0 38.5 39.0 35.0 39.0 14-15 39.027528008147826 40.5 38.0 41.0 35.5 41.0 16-17 39.049396187981955 40.0 38.5 41.0 35.5 41.0 18-19 39.40021097046414 41.0 39.0 41.0 36.0 41.0 20-21 39.48420631456423 41.0 39.0 41.0 36.0 41.0 22-23 39.36077404335806 41.0 39.0 41.0 35.0 41.0 24-25 39.25136039575149 41.0 39.0 41.0 35.0 41.0 26-27 38.9597119161938 41.0 38.0 41.0 35.0 41.0 28-29 38.67084970173141 40.5 38.0 41.0 35.0 41.0 30-31 37.9657645860614 39.5 36.5 41.0 34.5 41.0 32-33 37.078182744071 39.0 35.0 41.0 33.5 41.0 34-35 36.50835152044231 39.0 35.0 41.0 33.0 41.0 36-37 36.044172850283715 39.0 35.0 41.0 32.0 41.0 38-39 35.54993452640768 38.0 35.0 41.0 30.5 41.0 40-41 35.125156409137205 38.5 35.0 41.0 27.5 41.0 42-43 34.457202095154955 38.0 35.0 41.0 20.5 41.0 44-45 33.58075803870217 39.0 35.0 41.0 4.5 41.0 46-47 31.572166448421356 39.0 32.0 41.0 2.0 41.0 48-49 29.542303215480867 39.0 17.5 41.0 2.0 41.0 50-51 28.62690237159901 38.0 6.0 41.0 2.0 41.0 52-53 28.414273243125272 38.0 2.0 41.0 2.0 41.0 54-55 28.190368107085696 38.0 2.0 41.0 2.0 41.0 56-57 27.969933071438966 38.0 2.0 41.0 2.0 41.0 58-59 27.762498181289104 37.0 2.0 41.0 2.0 41.0 60-61 27.497846646297106 37.0 2.0 41.0 2.0 41.0 62-63 27.22512730976284 36.5 2.0 41.0 2.0 41.0 64-65 26.90583442455987 36.0 2.0 40.0 2.0 41.0 66-67 26.460432125709296 35.5 2.0 40.0 2.0 41.0 68-69 25.96728502837189 35.0 2.0 39.5 2.0 41.0 70-71 25.507376691401134 35.0 2.0 39.0 2.0 41.0 72-73 25.191939473301325 35.0 2.0 39.0 2.0 41.0 74-75 24.959428197293757 35.0 2.0 38.0 2.0 41.0 76-77 24.690935544885786 35.0 2.0 37.0 2.0 40.0 78-79 24.405026916921287 35.0 2.0 37.0 2.0 39.0 80-81 24.143903681070856 35.0 2.0 36.0 2.0 39.0 82-83 23.892797904845047 35.0 2.0 36.0 2.0 38.5 84-85 23.669067365051653 34.5 2.0 35.5 2.0 37.0 86-87 23.47267568747272 34.0 2.0 35.0 2.0 37.0 88-89 23.273861486978028 34.0 2.0 35.0 2.0 36.5 90-91 23.11129783209661 34.0 2.0 35.0 2.0 36.0 92-93 22.972064600611088 34.0 2.0 35.0 2.0 36.0 94-95 22.843969154663178 34.0 2.0 35.0 2.0 36.0 96-97 22.760221155245162 34.0 2.0 35.0 2.0 35.0 98-99 22.673032154808674 34.0 2.0 35.0 2.0 35.0 100-101 22.609668267132257 34.0 2.0 35.0 2.0 35.0 102-103 21.99216499345264 32.5 2.0 35.0 2.0 35.0 104-105 22.390077113342063 33.5 2.0 35.0 2.0 35.0 106-107 22.358235122944855 34.0 2.0 35.0 2.0 35.0 108-109 22.28846209806489 34.0 2.0 35.0 2.0 35.0 110-111 22.146762694602067 34.0 2.0 35.0 2.0 35.0 112-113 21.943918230758037 34.0 2.0 35.0 2.0 35.0 114-115 21.69098646879092 33.0 2.0 35.0 2.0 35.0 116-117 21.39857413065619 33.0 2.0 35.0 2.0 35.0 118-119 21.19476938745817 33.0 2.0 35.0 2.0 35.0 120-121 20.96102866288375 33.0 2.0 35.0 2.0 35.0 122-123 20.654604975993017 33.0 2.0 35.0 2.0 35.0 124-125 19.324065182598574 28.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19373884345690584 1101 2 0.04712754616846837 1101 3 0.35645288472535697 1101 4 0.24353967326653247 1101 5 -0.07812203821358565 1101 6 0.11222828029606546 1101 7 0.11169661325343583 1101 8 0.11173311437261191 1101 9 0.06056759262732214 1101 10-11 -0.03602922168332867 1101 12-13 -0.04651267201482767 1101 14-15 -0.1744610399742399 1101 16-17 0.6166617038674573 1101 18-19 -0.2849437765498948 1101 20-21 -0.03457780215673978 1101 22-23 0.11799226592125223 1101 24-25 0.3147883463020662 1101 26-27 0.3253319534339383 1101 28-29 0.2540719249424299 1101 30-31 -0.049983656220192074 1101 32-33 0.10990715676936702 1101 34-35 -0.09047112598590701 1101 36-37 0.07403943685199721 1101 38-39 -0.2805509465154046 1101 40-41 -0.5117381763673521 1101 42-43 -0.5343623793131371 1101 44-45 -0.7737410934918358 1101 46-47 -0.935229334567655 1101 48-49 0.29190587581432936 1101 50-51 0.2748810436258111 1101 52-53 0.5588146892415864 1101 54-55 0.6141842976374399 1101 56-57 0.47164607309145623 1101 58-59 0.6423680733709034 1101 60-61 0.9524470732220749 1101 62-63 0.7203803525777488 1101 64-65 0.6403072173527775 1101 66-67 0.6066079701561549 1101 68-69 1.067676973296006 1101 70-71 0.914847373520324 1101 72-73 0.6955337814518927 1101 74-75 1.2371301865962288 1101 76-77 1.6099273324087129 1101 78-79 1.3029460192776838 1101 80-81 1.0877393056243925 1101 82-83 0.8673918503828517 1101 84-85 0.5911736678183743 1101 86-87 0.6924839671464511 1101 88-89 0.743560233411813 1101 90-91 0.8238364144861308 1101 92-93 0.946790336949114 1101 94-95 1.002617407048863 1101 96-97 0.6110063665758148 1101 98-99 0.8661971618560145 1101 100-101 0.8190266472874583 1101 102-103 0.6531664399873307 1101 104-105 0.8628906716990699 1101 106-107 1.0302840320832445 1101 108-109 1.226538996202386 1101 110-111 0.8129656740237223 1101 112-113 0.4863411081247442 1101 114-115 0.4122354212062831 1101 116-117 0.16455255829222182 1101 118-119 0.29380456154106227 1101 120-121 0.3590986805457419 1101 122-123 0.3930282428023695 1101 124-125 0.3440595212939215 1102 1 0.2712212091649917 1102 2 0.22748565794275066 1102 3 0.13301284131787128 1102 4 0.27197157777006 1102 5 0.15497617122753837 1102 6 0.19372583862487858 1102 7 0.034648322423265654 1102 8 0.13028981540353612 1102 9 0.019330479225260433 1102 10-11 -0.14818331826499076 1102 12-13 0.23015580329716556 1102 14-15 0.2903788949145323 1102 16-17 0.08329219762763529 1102 18-19 0.24462757450816497 1102 20-21 0.13856381477217639 1102 22-23 -0.25536638844663173 1102 24-25 -0.26692624946569765 1102 26-27 -0.8203544274667678 1102 28-29 -0.7572140218833994 1102 30-31 -0.6091806176960475 1102 32-33 -0.5915578459436048 1102 34-35 -0.6871613050417906 1102 36-37 -0.05943858810730518 1102 38-39 -0.574365379505096 1102 40-41 -0.6980648177129822 1102 42-43 0.22771032822891613 1102 44-45 0.20053996999162393 1102 46-47 0.4382378385197008 1102 48-49 0.46423360234715716 1102 50-51 1.0703232574076864 1102 52-53 1.3235461054108733 1102 54-55 1.391558144626046 1102 56-57 0.8255256173128345 1102 58-59 0.4691721970822442 1102 60-61 0.1475637308455191 1102 62-63 0.010667927184368153 1102 64-65 0.50634085815582 1102 66-67 0.520444106889741 1102 68-69 1.2904116450268823 1102 70-71 0.7923297392284105 1102 72-73 0.7789304173715905 1102 74-75 0.42155775035097776 1102 76-77 0.764295210867747 1102 78-79 0.5293703274708434 1102 80-81 0.13630143259129923 1102 82-83 0.8329371569482369 1102 84-85 0.6500450731357894 1102 86-87 0.7180401255837765 1102 88-89 0.5136633261953207 1102 90-91 0.4285027194237294 1102 92-93 0.5560686874645739 1102 94-95 0.45720232294685204 1102 96-97 0.38246594335280903 1102 98-99 0.1411293376563414 1102 100-101 0.22358443350558233 1102 102-103 0.7362917791083312 1102 104-105 0.10629815949071286 1102 106-107 -0.25023685777025406 1102 108-109 -0.1526796690173633 1102 110-111 -0.04693665912874323 1102 112-113 0.25286308316544037 1102 114-115 0.1992131748687882 1102 116-117 0.1251060037615659 1102 118-119 0.37806934721659147 1102 120-121 -0.22842166671415853 1102 122-123 -0.6786483714135834 1102 124-125 -0.6504602833723823 1103 1 -0.17417352767711236 1103 2 -0.040716096443219385 1103 3 -0.09264505341896978 1103 4 -0.08614245731765635 1103 5 0.0684630133328028 1103 6 -0.14432240698915422 1103 7 -0.041009572313576825 1103 8 -0.12387685126312675 1103 9 -0.10566952077473957 1103 10-11 -0.44308463405446474 1103 12-13 -0.3965985826677496 1103 14-15 -0.20748294719073357 1103 16-17 -0.0795367497407895 1103 18-19 -0.48645795180762974 1103 20-21 -0.6702629396137851 1103 22-23 -0.15267998493786195 1103 24-25 -0.17876835472885944 1103 26-27 -0.2806066204492197 1103 28-29 0.034946065835896434 1103 30-31 -0.05583631945042811 1103 32-33 0.1847031189686703 1103 34-35 -0.445604287497936 1103 36-37 -0.24200437758099014 1103 38-39 -0.714990379505096 1103 40-41 -0.33309771244982755 1103 42-43 -0.08698265422722073 1103 44-45 -0.4635499422890845 1103 46-47 -0.17492005621713957 1103 48-49 -0.3415778011616162 1103 50-51 -0.3940407776800292 1103 52-53 0.025739087867012955 1103 54-55 -0.1235734343213224 1103 56-57 0.44882605590932556 1103 58-59 -0.3808278029177572 1103 60-61 -0.24267749722465837 1103 62-63 -0.43286277457001887 1103 64-65 -0.13170738745821353 1103 66-67 -0.003733524689206291 1103 68-69 0.06069673274617671 1103 70-71 -0.9843149976136942 1103 72-73 -1.2918480914003432 1103 74-75 -1.2992207584209545 1103 76-77 -1.4308802277287427 1103 78-79 -0.9261669532309114 1103 80-81 -1.1720867253034406 1103 82-83 -1.1105935448061501 1103 84-85 -1.0020930847589469 1103 86-87 -0.6672668919600824 1103 88-89 -0.909253340471345 1103 90-91 -0.7571880700499527 1103 92-93 -0.7387229792020875 1103 94-95 -0.4234994314391116 1103 96-97 -0.3510318636647334 1103 98-99 -0.8441228553261162 1103 100-101 -0.5328848647400299 1103 102-103 -0.44632883492676 1103 104-105 -0.7406316650706906 1103 106-107 -1.3637236998755178 1103 108-109 -1.4689077391928045 1103 110-111 -0.9197985012340091 1103 112-113 -0.7880360396415789 1103 114-115 -0.9379578777627913 1103 116-117 -0.5138194348349252 1103 118-119 -0.5212727580465675 1103 120-121 -1.5201431579422326 1103 122-123 -0.9106659152732313 1103 124-125 -0.9683330903899297 1104 1 -0.475920839505072 1104 2 -0.3370870641851553 1104 3 0.1902850541079104 1104 4 -0.03170697344668838 1104 5 -0.1502869866671972 1104 6 0.06367490483879834 1104 7 -0.12098269059314504 1104 8 0.08512852508095392 1104 9 0.33115843621450836 1104 10-11 0.20258068852618294 1104 12-13 0.38431539582688146 1104 14-15 0.21069581625013 1104 16-17 0.4570895943452271 1104 18-19 0.06041704819237026 1104 20-21 0.5987222754399752 1104 22-23 0.6186573806535378 1104 24-25 0.6854789570990931 1104 26-27 0.5599511752497008 1104 28-29 0.4245630013197754 1104 30-31 0.0710117988291401 1104 32-33 0.22519371036652558 1104 34-35 0.889409153362287 1104 36-37 1.118546697687826 1104 38-39 1.167570104365872 1104 40-41 1.1774869649695319 1104 42-43 1.0548184210415954 1104 44-45 1.4909191437324267 1104 46-47 2.1718541373312448 1104 48-49 1.170854994537308 1104 50-51 0.8139565341479233 1104 52-53 0.5781584427057247 1104 54-55 0.6916147377216859 1104 56-57 0.4365612709630895 1104 58-59 0.6810001540714907 1104 60-61 0.44784669632372953 1104 62-63 0.5768818490858969 1104 64-65 0.7913436878106026 1104 66-67 1.1886387871387498 1104 68-69 1.5872424316709122 1104 70-71 1.073144679805658 1104 72-73 0.9465591666641728 1104 74-75 0.8827348867403373 1104 76-77 0.9199262238841577 1104 78-79 1.0934903048336082 1104 80-81 1.192328597277207 1104 82-83 1.108828498204602 1104 84-85 0.8942442808324529 1104 86-87 0.803835258577557 1104 88-89 0.9082869821092956 1104 90-91 1.3441224138210153 1104 92-93 0.9603697627333929 1104 94-95 1.2431672352275562 1104 96-97 1.2623753943997826 1104 98-99 1.0838005317706596 1104 100-101 1.3498436298836225 1104 102-103 1.495035412385068 1104 104-105 1.4730780123486653 1104 106-107 1.9249187732427622 1104 108-109 0.4786729059684909 1104 110-111 0.5292942407014749 1104 112-113 0.4878376162724045 1104 114-115 0.5039104018071008 1104 116-117 0.788263898498407 1104 118-119 0.12187240324375281 1104 120-121 -0.29282460955513656 1104 122-123 -0.06523580774635107 1104 124-125 -0.2845293269490661 1105 1 0.27803235467582965 1105 2 0.24482311924305833 1105 3 0.2247569073653466 1105 4 -0.11677971221961769 1105 5 0.08500713097986079 1105 6 0.2901138675206454 1105 7 0.16364729043151982 1105 8 0.12244667814863419 1105 9 0.3624677341272218 1105 10-11 0.4530550718278832 1105 12-13 0.473499456547934 1105 14-15 0.8093675430053437 1105 16-17 0.5301446228082298 1105 18-19 0.7957111658394282 1105 20-21 0.6431561780332729 1105 22-23 0.6417440346699834 1105 24-25 0.6030943903691792 1105 26-27 0.8238664187664639 1105 28-29 0.7509387128947225 1105 30-31 0.9089308374123064 1105 32-33 1.6010634130863224 1105 34-35 1.5317241438746194 1105 36-37 1.5465985635954809 1105 38-39 1.71975226755373 1105 40-41 1.8632870914717472 1105 42-43 1.3505173457727722 1105 44-45 1.8008495675148382 1105 46-47 2.349589747704428 1105 48-49 3.273005532171723 1105 50-51 2.932552359574867 1105 52-53 2.925248891788584 1105 54-55 3.4242206833257356 1105 56-57 3.5496226245367772 1105 58-59 3.065250628454791 1105 60-61 3.096170541991029 1105 62-63 2.4513284019005717 1105 64-65 2.5037092792084508 1105 66-67 2.4886071615853034 1105 68-69 2.218172222942254 1105 70-71 2.7442389239549314 1105 72-73 2.724389653697699 1105 74-75 2.3885856141280684 1105 76-77 2.169609968349686 1105 78-79 2.0477913801024243 1105 80-81 1.95260690214754 1105 82-83 2.003989788527182 1105 84-85 2.0006642681822306 1105 86-87 2.179546833530118 1105 88-89 2.246996659528655 1105 90-91 1.9600300672049507 1105 92-93 2.0648922168763413 1105 94-95 2.3902260587569657 1105 96-97 2.4911862735901664 1105 98-99 2.7284874387915323 1105 100-101 2.661355331338399 1105 102-103 2.557654008210495 1105 104-105 2.6245644133606802 1105 106-107 2.438359633457818 1105 108-109 2.2884452019836665 1105 110-111 2.4132284595503037 1105 112-113 2.194194352515286 1105 114-115 2.2023607496881894 1105 116-117 2.1215972318317426 1105 118-119 2.4382860654828455 1105 120-121 2.4329818420577674 1105 122-123 2.6015889866875526 1105 124-125 2.7112012233355607 1106 1 -0.16058657115537045 1106 2 -0.3351001544142349 1106 3 -0.5301450534189698 1106 4 -0.25914970369446877 1106 5 -0.20463481275415063 1106 6 0.012833390112291454 1106 7 -0.3263356592701001 1106 8 -0.10666670633559505 1106 9 -0.1219738686008256 1106 10-11 -0.5239270253588089 1106 12-13 -0.19370003194310925 1106 14-15 -0.7869666428429056 1106 16-17 -0.211330228001664 1106 18-19 0.03324313514889354 1106 20-21 -0.02510895410654257 1106 22-23 -0.4681238255175728 1106 24-25 0.125579471358094 1106 26-27 -0.591521475521688 1106 28-29 -0.07397603561337718 1106 30-31 -0.37943233394318554 1106 32-33 -0.4835486926255328 1106 34-35 -0.4487745773529994 1106 36-37 -0.5966239427983808 1106 38-39 -0.574365379505096 1106 40-41 -0.5199183646237415 1106 42-43 -0.3632507701692518 1106 44-45 -1.2966568263470535 1106 46-47 -1.7890504909997489 1106 48-49 -1.0621393953645146 1106 50-51 -0.8455806327524975 1106 52-53 -0.4778841005387804 1106 54-55 -0.8599864777995805 1106 56-57 -0.9354583643805299 1106 58-59 -1.0656104116134095 1106 60-61 -0.9623332943261076 1106 62-63 -1.0682794412366832 1106 64-65 -0.5941168077480725 1106 66-67 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-3.328772690711716 2216 106-107 -2.894973699875518 2216 108-109 -2.851920559705622 2216 110-111 -2.928211962772469 2216 112-113 -3.173452706308243 2216 114-115 -2.815361723916638 2216 116-117 -2.149236101501593 2216 118-119 -2.141465065738874 2216 120-121 -2.0942617476858203 2216 122-123 -2.399447966555286 2216 124-125 -2.7431728339796706 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 6.0 5 20.0 6 49.0 7 130.0 8 217.0 9 468.0 10 924.0 11 1288.0 12 1526.0 13 2619.0 14 4863.0 15 5465.0 16 1481.0 17 799.0 18 598.0 19 684.0 20 884.0 21 882.0 22 750.0 23 271.0 24 297.0 25 371.0 26 431.0 27 455.0 28 552.0 29 593.0 30 747.0 31 954.0 32 1211.0 33 1600.0 34 2256.0 35 3194.0 36 5024.0 37 14978.0 38 11900.0 39 242.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.96053493211485 19.68887789403222 17.194662320464502 15.155924853388436 2 16.406943413796615 23.036070248956015 42.87688972310736 17.68009661414 3 16.154752724468565 33.9434590929593 36.760319515779365 13.141468666792766 4 8.884778936331333 22.895671928700416 38.26236383759539 29.957185297372867 5 7.327329075062929 40.63495758704477 34.45416054358422 17.58355279430808 6 19.73222731572437 43.019719129738775 17.885468966019065 19.36258458851779 7 17.161356030845337 43.407536737960136 20.686745234977447 18.744361996217084 8 25.53615597264659 32.054415830059654 20.190600902080604 22.218827295213153 9 28.52175178233668 12.520005819874871 20.884620980648915 38.07362141713953 10-11 22.0944274698094 29.32707696784519 25.913720355012366 22.664775207333044 12-13 27.875018187108978 29.529317619671176 28.059799214316893 14.535864978902953 14-15 23.367525098210386 26.08540666375673 33.902953586497894 16.64411465153499 16-17 14.053542848828751 30.397933944420195 33.69343809108104 21.855085115670015 18-19 13.76618652698967 21.469518405354286 47.65968281681944 17.104612250836606 20-21 17.07478539211407 19.285610359377273 49.54168485377564 14.097919394733013 22-23 15.927542557835006 21.057034773752363 48.882584024443474 14.132838643969153 24-25 14.848791275944102 21.961457598265667 49.872325566168826 13.317425559621416 26-27 14.723555943547215 21.562636403317327 50.31572821184345 13.398079441292014 28-29 13.542947351573925 21.033180803003077 50.683476527546404 14.740395317876603 30-31 14.348281581934058 22.263044553735124 49.77301166952827 13.61566219480255 32-33 15.705659828313692 20.683835297541105 50.13676705950822 13.473737814636985 34-35 14.868325331005384 21.232358504292158 49.26669576604103 14.632620398661428 36-37 14.559977884314595 22.083675859710898 48.22092405735528 15.135422198619224 38-39 14.83777098792376 21.113778553761094 48.42936126873273 15.619089189582425 40-41 15.336825258256948 20.840244434744655 48.2453077258839 15.577622581114506 42-43 16.387312672777536 20.668558126000292 47.246471700858436 15.697657500363743 44-45 15.767954836456758 21.901553951810033 45.377429868467004 16.95306134326621 46-47 18.474006605654093 22.383564434227182 40.605857789288365 18.536571170830364 48-49 20.304088462098065 24.134293612687326 35.656190891895825 19.905427033318784 50-51 19.449435831775293 25.73058148247841 34.17018892906248 20.649793756683813 52-53 18.30205150589262 27.324312527280664 34.62534555507057 19.748290411756148 54-55 17.971307599412185 27.10355163031617 33.39929287491452 21.525847895357128 56-57 18.40826422231922 27.009311799796304 33.035064746107956 21.547359231776518 58-59 16.669940417730636 27.713394006853054 34.23616112675237 21.380504448663945 60-61 17.983544184083982 28.613622971213232 32.42130380695335 20.98152903774944 62-63 16.972580516088666 29.02798693409575 32.55854558152731 21.440886968288265 64-65 16.896797567256908 28.935383899081902 32.899503848448255 21.26831468521294 66-67 16.90673650516514 28.591590280808965 33.32023861486978 21.18143459915612 68-69 17.23713980168632 28.235328352454186 32.97710590066856 21.55042594519093 70-71 17.541485700982854 27.552616454600347 33.0015932255178 21.904304618899 72-73 17.59604821871567 26.595226143448496 33.732003462901126 22.07672217493471 74-75 17.736068674523498 26.52480721664484 33.55958096900917 22.179543139822496 76-77 17.43005136114304 26.52373816001979 33.68156091315166 22.364649565685518 78-79 16.81810576244553 26.28565608654217 33.98831651619756 22.907921634814745 80-81 16.7337169628762 26.597749147018384 33.60129202162099 23.06724186848442 82-83 16.677700259713806 26.794898842563963 32.757404025927734 23.7699968717945 84-85 16.745234977447986 26.454968718172562 32.06387312672778 24.73592317765168 86-87 17.005565457786183 26.343166854606963 32.17198355825543 24.479284129351424 88-89 17.17387034113479 25.869933722799793 32.413261260212586 24.542934675852838 90-91 17.197878547576263 26.049631510407195 31.49512924417801 25.257360697838532 92-93 16.971742862963072 26.564909932194514 31.37313389401391 25.090213310828506 94-95 17.19155808725638 26.59668114392137 31.11955011385379 25.09221065496846 96-97 17.298528202777717 26.456700933423548 30.485045579879376 25.75972528391936 98-99 17.53617600197886 26.22205408394142 30.61119073428736 25.630579179792367 100-101 17.9278433769125 26.386857862074486 30.558526311194534 25.12677244981848 102-103 17.5152956924711 26.584313639901936 30.01156725375936 25.888823413867613 104-105 17.460733174738284 26.694166175604007 29.706017154455576 26.139083495202136 106-107 17.49308889858868 27.610213880401574 29.172850283718898 25.72384693729085 108-109 17.42901003281217 27.755021862654512 29.050047654038952 25.765920450494367 110-111 17.68916824897968 27.52133394443353 28.922499399812306 25.86699840677448 112-113 17.543923465861553 28.052089774835398 28.936742934051146 25.467243825251902 114-115 17.2588389349629 28.16382947766623 28.750909355448854 25.826422231922013 116-117 17.124254328531936 28.19511130510694 28.91241088316601 25.76822348319511 118-119 17.020951549541685 28.837479994180125 28.51229448566856 25.629273970609635 120-121 17.455386697124233 29.079943837798904 28.35027171738482 25.114397747692042 122-123 17.481230357350714 29.138778954720056 28.02351297869864 25.35647770923059 124-125 16.86389240644513 29.873513529249085 27.69820822962607 25.564385834679708 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 55.0 1 37.0 2 23.5 3 27.0 4 16.5 5 9.5 6 24.5 7 57.0 8 92.5 9 122.0 10 129.5 11 141.0 12 155.5 13 170.0 14 219.5 15 258.0 16 303.5 17 373.5 18 438.0 19 487.5 20 549.5 21 631.5 22 738.5 23 850.0 24 973.0 25 1106.5 26 1197.5 27 1366.5 28 1611.0 29 1823.0 30 1986.0 31 2144.5 32 2309.0 33 2400.0 34 2554.5 35 2668.0 36 2635.5 37 2752.0 38 2711.5 39 2395.5 40 2210.5 41 2122.0 42 2041.5 43 2013.0 44 2050.0 45 2038.0 46 1912.5 47 1773.5 48 1811.5 49 1913.5 50 1943.5 51 1817.0 52 1504.5 53 1192.0 54 897.0 55 650.5 56 509.0 57 390.5 58 277.5 59 220.5 60 157.5 61 106.5 62 93.0 63 91.0 64 62.5 65 44.0 66 35.5 67 20.5 68 32.5 69 56.5 70 60.0 71 42.5 72 27.5 73 20.5 74 17.5 75 16.0 76 13.5 77 7.0 78 4.5 79 6.5 80 4.0 81 0.5 82 0.5 83 1.0 84 0.5 85 1.0 86 1.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016004655899898152 2 0.004364906154517678 3 0.0014549687181725592 4 0.09020806052669868 5 0.0014549687181725592 6 0.021824530772588387 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 7.274843590862796E-4 26-27 0.0 28-29 7.274843590862796E-4 30-31 0.005819874872690237 32-33 0.0 34-35 0.0 36-37 7.274843590862796E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0029099374363451185 46-47 0.0014549687181725592 48-49 0.0 50-51 7.274843590862796E-4 52-53 0.0 54-55 0.0014549687181725592 56-57 0.0 58-59 0.002182453077258839 60-61 7.274843590862796E-4 62-63 0.002182453077258839 64-65 0.0014549687181725592 66-67 0.0 68-69 7.274843590862796E-4 70-71 0.002182453077258839 72-73 0.002182453077258839 74-75 0.0 76-77 0.0014549687181725592 78-79 7.274843590862796E-4 80-81 7.274843590862796E-4 82-83 7.274843590862796E-4 84-85 0.0 86-87 0.003637421795431398 88-89 0.0050923905136039576 90-91 0.0050923905136039576 92-93 0.005819874872690237 94-95 0.002182453077258839 96-97 0.006547359231776516 98-99 0.0050923905136039576 100-101 0.006547359231776516 102-103 0.002182453077258839 104-105 0.002182453077258839 106-107 0.0 108-109 0.008002327949949076 110-111 0.002182453077258839 112-113 0.003637421795431398 114-115 0.0 116-117 0.0 118-119 0.0 120-121 7.274843590862796E-4 122-123 0.0029099374363451185 124-125 0.0392841553906591 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 68730.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.30510694020079 #Duplication Level Percentage of deduplicated Percentage of total 1 77.87703476830582 39.95489596973665 2 10.461686801656175 10.734759202677143 3 4.350292099143553 6.695766041030119 4 2.359480460552436 4.842135894078278 5 1.3016845329249618 3.3391532082060236 6 0.9301797969485566 2.863378437363597 7 0.5501673189268901 1.9758475192783356 8 0.3658329079462311 1.501527717154081 9 0.3034427996143157 1.4011348756001745 >10 1.3498950711814417 11.617925214607885 >50 0.08224150643752481 2.805179688636694 >100 0.05388236628665418 6.000290993743635 >500 0.008507742045261188 2.981230903535574 >1k 0.005671828030174125 3.2867743343518114 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1199 1.7445074930888986 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1060 1.542266841262913 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 980 1.4258693438091081 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 553 0.8045977011494253 RNA PCR Primer, Index 46 (95% over 21bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 516 0.7507638585770405 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 412 0.5994471118870944 No Hit ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT 397 0.577622581114506 RNA PCR Primer, Index 46 (95% over 23bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 379 0.5514331441874 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 375 0.5456132693147098 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 348 0.5063291139240507 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 346 0.5034191764877055 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 246 0.3579223046704496 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 215 0.31281827440710025 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 149 0.21679033900771136 No Hit CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT 147 0.21388040157136623 RNA PCR Primer, Index 46 (96% over 26bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG 141 0.20515058926233085 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 140 0.2036956205441583 No Hit TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG 131 0.19060090208060526 RNA PCR Primer, Index 46 (96% over 28bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG 128 0.18623599592608758 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 123 0.1789611523352248 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 118 0.171686308744362 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 117 0.17023134002618945 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 109 0.15859159028080896 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 103 0.1498617779717736 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT 96 0.1396769969445657 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGG 95 0.13822202822639315 No Hit CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG 93 0.13531209079004802 TruSeq Adapter, Index 4 (95% over 23bp) TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 89 0.12949221591735777 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 80 0.11639749745380473 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 80 0.11639749745380473 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 79 0.11494252873563218 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT 74 0.1076676851447694 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA 73 0.10621271642659681 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT 70 0.10184781027207915 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGA 69 0.10039284155390658 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0014549687181725592 0.0 18-19 0.0 0.0 0.0 0.0014549687181725592 0.0 20-21 0.0 0.0 0.0 0.006547359231776517 0.0 22-23 0.0 0.0 0.0 0.012367234104466754 0.0 24-25 0.0 0.0 0.0 0.01964207769532955 0.0 26-27 0.0 0.0 0.0 0.03637421795431398 0.0 28-29 0.0 0.0 0.0 0.085843154372181 0.0 30-31 0.0 0.0 0.0 0.2226102138804016 0.0 32-33 0.0 0.0 0.0 0.43212570929725014 0.0 34-35 0.0 0.0 0.0 0.7071147970318639 0.0 36-37 0.0 0.0 0.0 1.0781318201658665 0.0 38-39 0.0 0.0 0.0 1.6499345264076823 0.0 40-41 0.0 0.0 0.0 2.4232504001163973 0.0 42-43 0.0 0.0 0.0 2.976865997381056 0.0 44-45 0.0 0.0 0.0 3.462825549250691 0.0 46-47 0.0 0.0 0.0 4.003346428051797 0.0 48-49 0.0 0.0 0.0 4.6020660555798045 0.0 50-51 0.0 0.0 0.0 5.315728211843446 0.0 52-53 0.0 0.0 0.0 5.908627964498764 0.0 54-55 0.0 0.0 0.0 6.433144187399971 0.0 56-57 0.0 0.0 0.0 7.026043940055288 0.0 58-59 0.0 0.0 0.0 7.737523643241671 0.0 60-61 0.0 0.0 0.0 8.526844172850282 0.0 62-63 0.0 0.0 0.0 9.285610359377273 0.0 64-65 0.0 0.0 0.0 9.981812891022843 0.0 66-67 0.0 0.0 0.0 10.698384984722829 0.0 68-69 0.0 0.0 0.0 11.47461079586789 0.0 70-71 0.0 0.0 0.0 12.43634511857995 0.0 72-73 0.0 0.0 0.0 13.398806925651098 0.0 74-75 0.0 0.0 0.0 14.27469809399098 0.0 76-77 0.0 0.0 0.0 15.094572966681216 0.0 78-79 0.0 0.0 0.0 16.09049905427033 0.0 80-81 0.0 0.0 0.0 17.0944274698094 0.0 82-83 0.0 0.0 0.0 18.13618507202095 0.0 84-85 0.0 0.0 0.0 19.160483049614434 0.0 86-87 0.0 0.0 0.0 20.149861777971772 0.0 88-89 0.0 0.0 0.0 21.091954022988507 0.0 90-91 0.0 0.0 0.0 22.147533828022695 0.0 92-93 0.0 0.0 0.0 23.30641641204714 0.0 94-95 0.0 0.0 0.0 24.414375090935543 0.0 96-97 0.0 0.0 0.0 25.419030990833697 0.0 98-99 0.0 0.0 0.0 26.451331296377127 0.0 100-101 0.0 0.0 0.0 27.55928997526553 0.0 102-103 0.0 0.0 0.0 28.68034337261749 0.0 104-105 0.0 0.0 0.0 29.855958096900917 0.0 106-107 0.0 0.0 0.0 30.908627964498763 0.0 108-109 0.0 0.0 0.0 31.95475047286483 0.0 110-111 0.0 0.0 0.0 33.0750763858577 0.0 112-113 0.0 0.0 0.0 34.179397642950676 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGATGT 15 2.6907783E-4 119.00001 1 GGTATCA 830 0.0 74.554214 1 GTATAAG 25 0.0020486002 71.4 8 TTTACAG 30 0.004219639 59.500004 3 GACTTTC 30 0.004219639 59.500004 6 AGATGTG 30 0.004219639 59.500004 2 GCATACG 30 0.004219639 59.500004 7 GTATCAA 1550 0.0 55.66129 1 TATCAAC 2075 0.0 41.578312 2 ATCAACG 2135 0.0 40.40984 3 TCAACGC 2140 0.0 40.315422 4 CAACGCA 2150 0.0 40.127907 5 AACGCAG 2185 0.0 39.485126 6 ATGTGTA 65 0.0019262546 36.615383 4 ACGCAGA 2375 0.0 36.326317 7 CGCAGAG 2365 0.0 36.22833 8 GCAGAGT 2430 0.0 35.014404 9 CATGGGT 105 1.4635492E-5 34.0 4 AGAGTAA 35 0.00278049 34.0 10-11 GATTGGC 35 0.00278049 34.0 16-17 >>END_MODULE