FastQCFastQC Report
Fri 27 May 2016
SRR940087_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940087_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660067
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17250.2613371066876544No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8350.056.446591
GTATCAA14400.051.789851
ATCAACG16200.043.0335583
TCAACGC16350.041.913074
CAACGCA16500.041.5194325
AACGCAG18050.037.624026
TATCAAC19750.036.541252
GTACATG36400.035.8954851
TACATGG36600.035.5255282
ACGCAGA21050.031.6959297
ACATGGG37750.030.778973
CGCAGAG21700.030.746518
GTACTAG1951.378794E-930.5958481
GAGTACA25500.026.672381
GCAGAGT25300.025.190399
AGTACAT25850.024.6881392
CATGGGA22300.024.5839884
CATGGGG16950.023.9060924
GGACCGT1250.001621437623.8285456
ATTCGCG650.002057473622.87474680-81
Produced by FastQC (version 0.11.5)