##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940087_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 660067 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.283318208606097 34.0 31.0 34.0 28.0 34.0 2 31.42380091717962 34.0 31.0 34.0 28.0 34.0 3 31.514099326280515 34.0 31.0 34.0 28.0 34.0 4 34.551572794882944 37.0 35.0 37.0 32.0 37.0 5 34.79298919655126 37.0 37.0 37.0 32.0 37.0 6 34.69012539636128 37.0 37.0 37.0 32.0 37.0 7 34.65028550132032 37.0 37.0 37.0 31.0 37.0 8 34.589641657589304 37.0 37.0 37.0 31.0 37.0 9 36.13123516249108 39.0 39.0 39.0 31.0 39.0 10-11 36.19579906282241 39.0 39.0 39.0 31.0 39.0 12-13 36.130045131782076 39.0 39.0 39.0 31.0 39.0 14-15 37.6198105646851 41.0 39.0 41.0 31.0 41.0 16-17 37.69225396815778 41.0 39.0 41.0 31.0 41.0 18-19 37.73180146863879 41.0 39.5 41.0 31.0 41.0 20-21 37.770456635462764 41.0 39.5 41.0 31.0 41.0 22-23 37.82988620852126 41.0 39.0 41.0 31.0 41.0 24-25 37.938947106884605 41.0 39.0 41.0 31.0 41.0 26-27 37.998941016593776 41.0 39.0 41.0 31.0 41.0 28-29 37.953819839501136 41.0 39.0 41.0 31.0 41.0 30-31 37.89998363802462 41.0 39.0 41.0 31.0 41.0 32-33 37.84863809279967 41.0 39.0 41.0 30.5 41.0 34-35 37.75337655116829 41.0 39.0 41.0 30.0 41.0 36-37 37.61944620773346 41.0 38.0 41.0 30.0 41.0 38-39 37.5135319596344 41.0 38.0 41.0 30.0 41.0 40-41 37.41273385883554 41.0 38.0 41.0 30.0 41.0 42-43 37.25188882340732 40.5 38.0 41.0 30.0 41.0 44-45 37.112721890353555 40.0 38.0 41.0 30.0 41.0 46-47 36.959051884126914 40.0 37.5 41.0 30.0 41.0 48-49 36.807597562065666 40.0 37.0 41.0 30.0 41.0 50-51 36.649043203189976 40.0 37.0 41.0 30.0 41.0 52-53 36.77660904120339 40.0 37.0 41.0 30.0 41.0 54-55 36.58225452870693 40.0 36.0 41.0 30.0 41.0 56-57 36.46548835194003 40.0 36.0 41.0 30.0 41.0 58-59 36.27465848163899 40.0 35.0 41.0 30.0 41.0 60-61 36.02748887612924 39.0 35.0 41.0 30.0 41.0 62-63 35.74108916216081 39.0 35.0 41.0 29.5 41.0 64-65 35.42660593545807 38.5 35.0 41.0 29.0 41.0 66-67 35.099060398414096 37.5 35.0 40.5 29.0 41.0 68-69 34.74577883760285 37.0 35.0 40.0 28.0 41.0 70-71 34.37523387777301 36.5 35.0 39.5 28.0 41.0 72-73 34.002981515512815 36.0 34.5 39.0 28.0 41.0 74-75 33.62471158230907 35.5 34.0 39.0 27.0 40.5 76-77 33.24951330698247 35.0 34.0 37.0 27.0 39.5 78-79 32.905529287178425 35.0 34.0 37.0 26.0 39.0 80-81 32.580061569507336 35.0 34.0 36.5 26.0 39.0 82-83 32.270172573390276 35.0 34.0 36.0 25.5 37.5 84-85 32.01434551341001 35.0 34.0 36.0 25.0 37.0 86-87 31.79366943052751 35.0 33.0 35.0 25.0 37.0 88-89 31.613962673486178 35.0 33.0 35.0 25.0 36.0 90-91 31.461962952245756 35.0 33.0 35.0 24.0 36.0 92-93 31.31178804575899 35.0 33.0 35.0 24.0 36.0 94-95 31.19537940845399 35.0 33.0 35.0 23.0 36.0 96-97 31.090577926180224 35.0 33.0 35.0 23.0 35.0 98-99 31.01674829979381 35.0 33.0 35.0 22.0 35.0 100-101 30.921162548650365 35.0 33.0 35.0 20.0 35.0 102-103 30.240304393341887 34.0 31.0 35.0 21.5 35.0 104-105 31.11155912960351 35.0 33.0 35.0 23.5 35.0 106-107 31.22718451308731 35.0 34.0 35.0 24.0 35.0 108-109 31.213071551827312 35.0 34.0 35.0 23.5 35.0 110-111 31.157943814794557 35.0 34.0 35.0 23.0 35.0 112-113 31.104453032798187 35.0 34.0 35.0 22.0 35.0 114-115 31.040558003960207 35.0 33.5 35.0 20.0 35.0 116-117 30.96444148851556 35.0 33.0 35.0 19.5 35.0 118-119 30.892862391242105 35.0 33.0 35.0 18.5 35.0 120-121 30.799911221133613 35.0 33.0 35.0 16.5 35.0 122-123 30.673766450981493 35.0 33.0 35.0 2.0 35.0 124-125 29.46036993214325 34.5 30.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.1431920333269474 1101 2 0.06578167927013823 1101 3 0.06494572115468245 1101 4 -0.0021469375023031034 1101 5 0.08029942885459462 1101 6 -0.020541771548188592 1101 7 0.015396296864771841 1101 8 -0.003373076158069921 1101 9 0.06321061425282437 1101 10-11 0.03888497895303544 1101 12-13 -0.07231776033622594 1101 14-15 0.03166727321801943 1101 16-17 -0.0490149308828407 1101 18-19 -0.0976453941177482 1101 20-21 -0.21290432841619378 1101 22-23 -0.21616522321831155 1101 24-25 -0.1291547708666485 1101 26-27 -0.04166353663855915 1101 28-29 -0.1414419159766851 1101 30-31 -0.07107298894137415 1101 32-33 0.01724410259286202 1101 34-35 0.014985314082061052 1101 36-37 0.08134312533114496 1101 38-39 0.133917923053815 1101 40-41 0.05570502268626143 1101 42-43 0.15202697872782522 1101 44-45 0.09508570658558568 1101 46-47 0.1378060505638743 1101 48-49 0.06733716831007541 1101 50-51 0.12021870716777272 1101 52-53 0.1615706075700345 1101 54-55 0.0644734242945404 1101 56-57 0.037513714193771364 1101 58-59 0.17887605757972835 1101 60-61 0.06590804700290676 1101 62-63 0.050684829811004306 1101 64-65 0.05127055548671677 1101 66-67 -0.004104532871629374 1101 68-69 0.1086527302804896 1101 70-71 -0.031047191133225738 1101 72-73 -0.0017863631158618887 1101 74-75 0.026232534374571514 1101 76-77 0.00525478531910295 1101 78-79 0.11627256609924785 1101 80-81 0.07664978083715823 1101 82-83 0.0036936402711233995 1101 84-85 -0.17462514262133766 1101 86-87 -0.22578945086091196 1101 88-89 -0.068789971153425 1101 90-91 -0.12673770614987845 1101 92-93 -0.21401582023131027 1101 94-95 -0.19954977744021463 1101 96-97 -0.20410355433705618 1101 98-99 -0.2829396215342257 1101 100-101 -0.1869338685294082 1101 102-103 -0.1540666883468127 1101 104-105 -0.11729912438103796 1101 106-107 -0.1292697461005261 1101 108-109 -0.10451537792670251 1101 110-111 -0.011499072096928131 1101 112-113 0.02164758846261705 1101 114-115 -0.029706500195000984 1101 116-117 0.002445241848207047 1101 118-119 -0.044890122778223684 1101 120-121 -0.11920030638432522 1101 122-123 -0.1276707014074283 1101 124-125 -0.22599724862028125 1102 1 0.26904706815862767 1102 2 0.4825938933552649 1102 3 0.43313772476680157 1102 4 0.23468629642658811 1102 5 0.31780840957522116 1102 6 0.5187102698020212 1102 7 0.3497246915008958 1102 8 0.3434207875119242 1102 9 0.4135464283751702 1102 10-11 0.3823062102155532 1102 12-13 0.398437504921084 1102 14-15 0.5271292445376758 1102 16-17 0.43133959974770875 1102 18-19 0.39647194786351747 1102 20-21 0.4214632686861748 1102 22-23 0.4406133713198841 1102 24-25 0.5139609252211912 1102 26-27 0.522694862375424 1102 28-29 0.5907899856785122 1102 30-31 0.6703806446086205 1102 32-33 0.62383438800817 1102 34-35 0.6396224624611051 1102 36-37 0.5640513293243146 1102 38-39 0.6287872812163968 1102 40-41 0.6437610837383829 1102 42-43 0.634067062868219 1102 44-45 0.5935793861412719 1102 46-47 0.5965290569201542 1102 48-49 0.628769110845667 1102 50-51 0.6793907876899397 1102 52-53 0.5583880384814108 1102 54-55 0.8010290376453426 1102 56-57 0.7052651858116477 1102 58-59 0.7530099042568992 1102 60-61 0.7525117863385375 1102 62-63 0.8393980279237994 1102 64-65 0.6816240946152021 1102 66-67 0.6870555397978464 1102 68-69 0.7669223913850871 1102 70-71 0.5817796422684864 1102 72-73 0.5832410404758193 1102 74-75 0.6329883243652858 1102 76-77 0.7191721184531161 1102 78-79 0.5902593653932939 1102 80-81 0.5818284723900149 1102 82-83 0.4601155991436201 1102 84-85 0.4128901684586239 1102 86-87 0.5195638365785875 1102 88-89 0.5840096493300884 1102 90-91 0.556488824614906 1102 92-93 0.5531253437311001 1102 94-95 0.405987227704923 1102 96-97 0.5454491256930218 1102 98-99 0.5945160621369574 1102 100-101 0.6175068404098241 1102 102-103 0.61804800251047 1102 104-105 0.7019897093227137 1102 106-107 0.7308735488286295 1102 108-109 0.6186366528983314 1102 110-111 0.5754838786437269 1102 112-113 0.6366511502431109 1102 114-115 0.612725822162151 1102 116-117 0.5132381231684597 1102 118-119 0.6533462238570031 1102 120-121 0.6606740321693927 1102 122-123 0.6376117775307826 1102 124-125 0.6682300738207623 1103 1 -0.414949384110308 1103 2 -0.26653042384440795 1103 3 -0.2600067639839594 1103 4 -0.598716956047312 1103 5 -0.20980626386368328 1103 6 -0.32137038156372455 1103 7 -0.3889509480414972 1103 8 -0.32330442736242304 1103 9 -0.14941781415376454 1103 10-11 -0.39642874204830036 1103 12-13 -0.18830626150930385 1103 14-15 -0.25176175563505865 1103 16-17 -0.29799203617147185 1103 18-19 -0.2337583788499984 1103 20-21 -0.3981961549381694 1103 22-23 -0.3759396102112973 1103 24-25 -0.36106647388839264 1103 26-27 -0.2321524211570889 1103 28-29 -0.16573172820655202 1103 30-31 -0.16031027746759463 1103 32-33 0.017364714286195237 1103 34-35 6.040981625474728E-4 1103 36-37 0.07040356792228408 1103 38-39 0.042914479858850996 1103 40-41 0.12802056745932333 1103 42-43 -0.015272963347825907 1103 44-45 -0.035327799465740384 1103 46-47 -0.03971746103406559 1103 48-49 -0.19022257288949618 1103 50-51 -0.2594507706988338 1103 52-53 -0.1499809197427524 1103 54-55 -0.0829417211539436 1103 56-57 -0.019594770262678196 1103 58-59 -0.08046520283551217 1103 60-61 -0.13658823093560102 1103 62-63 -0.12372966571713562 1103 64-65 -0.19841716009707966 1103 66-67 -0.17779535823972736 1103 68-69 -0.06493054325914471 1103 70-71 -0.11620596067885458 1103 72-73 -0.23223920240731388 1103 74-75 -0.18192942024163017 1103 76-77 -0.09845360172612772 1103 78-79 -0.08265913695323235 1103 80-81 -0.2222421280387863 1103 82-83 -0.12101116837112613 1103 84-85 -0.27611796200033467 1103 86-87 -0.20472926639416045 1103 88-89 -0.09039021944450454 1103 90-91 0.002201571624226517 1103 92-93 -0.01859645260533327 1103 94-95 -0.047284044528275615 1103 96-97 -0.06543851836097048 1103 98-99 -0.057062344316314295 1103 100-101 -0.042410330811748054 1103 102-103 0.0776998197065133 1103 104-105 0.10084671282544377 1103 106-107 0.03347076667586535 1103 108-109 0.07209010199260035 1103 110-111 0.08266716308204991 1103 112-113 0.06454540347039739 1103 114-115 -0.032575829332788686 1103 116-117 0.01308134945534789 1103 118-119 -0.029532489901779257 1103 120-121 -0.02295371155203796 1103 122-123 0.021880116063293542 1103 124-125 -0.001964485260426585 1104 1 -0.34780812462270916 1104 2 -0.13399640473580376 1104 3 -0.1458178011055189 1104 4 -0.013110005192650931 1104 5 -0.28089867889396913 1104 6 0.028136575535583574 1104 7 0.01948414457790193 1104 8 -0.00609930221411048 1104 9 0.09094414802641637 1104 10-11 0.12228318692172735 1104 12-13 0.07527725490558623 1104 14-15 0.010445810600728578 1104 16-17 -0.06357667662199162 1104 18-19 0.059580148890404416 1104 20-21 -0.0029677713354629986 1104 22-23 0.004090874997416449 1104 24-25 0.09021396164702367 1104 26-27 -0.024563477580272775 1104 28-29 -0.05372768054290589 1104 30-31 -0.08565486818805113 1104 32-33 0.15336450991608075 1104 34-35 0.07328747776299593 1104 36-37 0.055285146794957996 1104 38-39 0.17794990401198874 1104 40-41 0.20828196251243014 1104 42-43 0.22807720213519644 1104 44-45 0.07900947366923816 1104 46-47 0.21797693184478106 1104 48-49 0.16277073966621458 1104 50-51 0.11239497528047337 1104 52-53 0.09665123108239015 1104 54-55 0.22441177102037813 1104 56-57 0.04881457739926276 1104 58-59 -0.019506848923242615 1104 60-61 0.07301815790124522 1104 62-63 0.06761114415620995 1104 64-65 0.04050285738791359 1104 66-67 0.17168037564768213 1104 68-69 0.09593357332264674 1104 70-71 0.16304876963775428 1104 72-73 0.2506199638761615 1104 74-75 0.31027754991465883 1104 76-77 0.1787482024078244 1104 78-79 0.27337134257853535 1104 80-81 0.12904313290211178 1104 82-83 0.33222923332968435 1104 84-85 0.34596621577895803 1104 86-87 0.20810404139291094 1104 88-89 0.07165541186045488 1104 90-91 0.04788467873119728 1104 92-93 0.07551669463765975 1104 94-95 -0.020704575777035217 1104 96-97 0.10939230859998972 1104 98-99 0.12322331968786315 1104 100-101 0.1271466118113409 1104 102-103 0.10601572481065702 1104 104-105 0.12819469836058062 1104 106-107 0.14965091893159865 1104 108-109 0.1915799147101005 1104 110-111 0.13937845055700038 1104 112-113 0.11924066492221996 1104 114-115 0.13896307762771798 1104 116-117 0.22498761169626746 1104 118-119 0.2767956036089032 1104 120-121 0.32985297135070013 1104 122-123 0.2993863465133053 1104 124-125 0.2270655798541661 1105 1 0.14296712798583755 1105 2 0.2985661839108289 1105 3 0.2596092970022532 1105 4 0.24673045835366025 1105 5 0.10783261597909188 1105 6 0.06392507703639438 1105 7 0.20054042763141666 1105 8 0.09557082785592996 1105 9 0.29410848926509914 1105 10-11 0.24155482769533165 1105 12-13 0.20649351421004525 1105 14-15 0.40183650449084496 1105 16-17 0.3072768598976694 1105 18-19 0.4037338690254231 1105 20-21 0.248751561052309 1105 22-23 0.2772791851864156 1105 24-25 0.25051456306977116 1105 26-27 0.2921977454958906 1105 28-29 0.39436387425033104 1105 30-31 0.4229390450179622 1105 32-33 0.547343135769566 1105 34-35 0.5630869302154196 1105 36-37 0.5126619211020085 1105 38-39 0.5281535851150068 1105 40-41 0.5241017445928904 1105 42-43 0.45404915406535906 1105 44-45 0.3091787406371722 1105 46-47 0.2966058089324406 1105 48-49 0.4333023995709908 1105 50-51 0.3553004958354933 1105 52-53 0.3784980496990116 1105 54-55 0.302086837814592 1105 56-57 0.31751008377083423 1105 58-59 0.20470041652738757 1105 60-61 0.203916938563367 1105 62-63 0.2185234485838663 1105 64-65 0.13716204750127048 1105 66-67 0.1265296901137063 1105 68-69 0.1203162079280915 1105 70-71 0.14772733282012496 1105 72-73 0.17915153582149657 1105 74-75 0.10550519996797902 1105 76-77 0.09167521805361645 1105 78-79 0.16404461719893249 1105 80-81 0.1982012510096638 1105 82-83 0.15926247100711066 1105 84-85 0.1905061328771822 1105 86-87 0.26484063582287476 1105 88-89 0.27903356053391803 1105 90-91 0.25980785714594745 1105 92-93 0.313486865388942 1105 94-95 0.37992598353512363 1105 96-97 0.3015548466699407 1105 98-99 0.17442671492266015 1105 100-101 0.21777645645295962 1105 102-103 0.2545091443442544 1105 104-105 0.26406043925402756 1105 106-107 0.3109465616403142 1105 108-109 0.28079003884287346 1105 110-111 0.39159392922133307 1105 112-113 0.30844281772977666 1105 114-115 0.38037878498662536 1105 116-117 0.4013573052675312 1105 118-119 0.2881148854199864 1105 120-121 0.2646090487989987 1105 122-123 0.25615427649757905 1105 124-125 0.1446681900508615 1106 1 0.15701865023408246 1106 2 0.19669264761106575 1106 3 0.25843833681490125 1106 4 0.16359228505154988 1106 5 0.2998700867050914 1106 6 0.1739953346476355 1106 7 0.17477930350963788 1106 8 0.18456380209480727 1106 9 0.41588834874988123 1106 10-11 0.3118124389365491 1106 12-13 0.19888227299224326 1106 14-15 0.2115554740458805 1106 16-17 0.3359653844878352 1106 18-19 0.45408515708162867 1106 20-21 0.31491081163778745 1106 22-23 0.4025719252332536 1106 24-25 0.45484711576298764 1106 26-27 0.5568347478378115 1106 28-29 0.5588837805735238 1106 30-31 0.5294964220671403 1106 32-33 0.5063595292121903 1106 34-35 0.4500892721357914 1106 36-37 0.5202731623198034 1106 38-39 0.43623321040774243 1106 40-41 0.4315958897919572 1106 42-43 0.3919882641356125 1106 44-45 0.44032628162077714 1106 46-47 0.26674632415492283 1106 48-49 0.3132789803672438 1106 50-51 0.37403585883315316 1106 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52-53 -0.9430550919622647 2216 54-55 -0.756584536267404 2216 56-57 -0.7839214291225929 2216 58-59 -0.7641301020544731 2216 60-61 -0.8458311884629097 2216 62-63 -0.7747497588504828 2216 64-65 -0.8351307198706479 2216 66-67 -0.729488016540266 2216 68-69 -0.7725373183738995 2216 70-71 -0.9083742779131683 2216 72-73 -0.8736397214377334 2216 74-75 -0.7802269274183402 2216 76-77 -0.7768107633749679 2216 78-79 -0.7938349724104583 2216 80-81 -0.7898227275546148 2216 82-83 -0.8607141740834514 2216 84-85 -0.8855235003719848 2216 86-87 -0.9341072681021316 2216 88-89 -0.881135228112992 2216 90-91 -1.0192571910185286 2216 92-93 -1.0311929408922929 2216 94-95 -1.0374200597124492 2216 96-97 -1.1266685889288581 2216 98-99 -1.156372350880961 2216 100-101 -1.1545205704172403 2216 102-103 -1.225626323774609 2216 104-105 -1.1270378522853228 2216 106-107 -1.2480430692942548 2216 108-109 -1.132649723010985 2216 110-111 -1.138874342338557 2216 112-113 -1.1559562152837444 2216 114-115 -1.1267357826582263 2216 116-117 -1.2451806870460835 2216 118-119 -1.2821154007379292 2216 120-121 -1.3376410110750818 2216 122-123 -1.3072415241193234 2216 124-125 -1.3334215133755407 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12475.0 3 2425.0 4 1920.0 5 1356.0 6 1459.0 7 1577.0 8 1583.0 9 1699.0 10 1991.0 11 2353.0 12 2795.0 13 2661.0 14 2513.0 15 2527.0 16 2115.0 17 2078.0 18 1769.0 19 1572.0 20 1337.0 21 1380.0 22 1332.0 23 2038.0 24 4074.0 25 6545.0 26 8106.0 27 5591.0 28 5052.0 29 6256.0 30 6646.0 31 7925.0 32 10405.0 33 14049.0 34 21172.0 35 37944.0 36 93844.0 37 237390.0 38 141059.0 39 1054.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.75664094413953 19.152748157209054 13.550784938755529 23.539825959895897 2 14.901833980790338 20.28627770668148 41.030405431048486 23.781482881479693 3 19.71310196003552 25.489402715014414 27.45368139345281 27.34381393149726 4 12.244870103280405 17.152281723463307 36.74037338671762 33.86247478653866 5 13.60916976305788 39.46898658600896 32.76199106542124 14.15985258551192 6 31.557274561125652 36.88651178439952 17.501625460547153 14.054588193927673 7 28.538171589140298 33.98242186611943 19.383386102088977 18.0960204426513 8 25.42721950923595 37.848888349661806 18.66419970275107 18.05969243835118 9 25.5386696963124 19.30109834770039 19.144711594613987 36.015520361373234 10-11 24.793960884587023 29.01220206270258 25.721942114281514 20.471894938428882 12-13 24.99914755484632 25.885311650159544 26.39155041209093 22.72399038290321 14-15 22.292594085329082 27.10307491371029 24.35619846481592 26.24813253614471 16-17 22.460940903866014 30.15046248061158 25.02093229605124 22.367664319471164 18-19 22.40055721157745 28.90451688498113 26.737742974791328 21.957182928650088 20-21 23.592081077430038 27.91043779322948 26.62094905035768 21.876532078982798 22-23 23.074484824356862 27.934027185349485 26.285844281408178 22.705643708885475 24-25 22.645891861651563 27.982992232947883 26.74228032644967 22.628835578950877 26-27 22.58451141718563 28.305529039198753 26.42476173159386 22.68519781202176 28-29 22.34613933630189 28.313907419219458 26.602543221585716 22.737410022892934 30-31 22.604461878431277 28.17745394601777 26.57112316642008 22.646961009130873 32-33 22.518497307313247 28.18847973989002 26.391814898902094 22.901208053894642 34-35 22.817825419973108 28.2427226757069 26.359879547735837 22.579572356584155 36-37 22.738098484641885 28.09169719502698 26.296205392990142 22.87399892734099 38-39 22.61499464401243 28.116889315145706 26.457896786255947 22.810219254585924 40-41 22.9258108355845 27.895668220638793 26.482245477092675 22.69627546668404 42-43 22.47578225201003 28.09175433692196 26.375849145797396 23.056614265270607 44-45 22.59242171833677 28.22359544518257 26.487092133462724 22.69689070301793 46-47 22.75498097614859 28.211212480737498 26.206927379042096 22.826879164071816 48-49 22.56248669427943 28.164034059614174 26.388094438985366 22.88538480712103 50-51 22.702121212121213 28.272348484848486 26.409015151515153 22.616515151515152 52-53 22.774653531955064 28.1845021413479 26.391270058327393 22.649574268369644 54-55 22.753919419136555 28.032540232096885 26.53583501393218 22.677705334834382 56-57 22.601809320553993 28.267432760354044 26.458112206360422 22.672645712731534 58-59 22.69043870641233 28.073013184072444 26.477166144162627 22.759381965352603 60-61 22.610013795046964 28.19757598070966 26.55148825148348 22.640921972759894 62-63 22.378824520571193 28.356380464033748 26.64330133100589 22.62149368438917 64-65 22.398629423892793 28.201831122413022 26.600981862295292 22.798557591398893 66-67 22.497348083042883 28.475753902106383 26.220639490831942 22.80625852401879 68-69 22.598877334214333 28.140827268897244 26.519720909650257 22.740574487238163 70-71 22.480817503559187 28.088160306344108 26.58417075623019 22.84685143386651 72-73 22.588003733491725 28.23437964034838 26.460971071500005 22.716645554659895 74-75 22.446672873676242 28.45706822584323 26.299134932762026 22.7971239677185 76-77 22.569139837243164 28.36896195926382 26.39972848608264 22.662169717410375 78-79 22.637925482545036 28.21783190953346 26.47638891519709 22.667853692724414 80-81 22.636045840322573 28.502310047988992 26.325455380237383 22.53618873145105 82-83 22.683308608825968 28.474828734170888 26.390393657595627 22.45146899940752 84-85 22.77424775388709 28.343789561247533 26.328860173931222 22.55310251093415 86-87 22.62591991223092 28.367505741282763 26.38845848736236 22.61811585912396 88-89 22.590932682727242 28.339552733568468 26.375459825200124 22.694054758504162 90-91 22.663568219754342 28.407691765652547 26.400495226174247 22.52824478841886 92-93 22.589904072014185 28.329519450800916 26.53143801051722 22.549138466667674 94-95 22.412003418637635 28.348022160530494 26.563238734861617 22.67673568597025 96-97 22.623950974241474 28.03031680653677 26.73541971638041 22.61031250284135 98-99 22.465185849747144 28.212153771779207 26.376539907647217 22.946120470826433 100-101 22.313510466537313 28.360607422077734 26.51368730173282 22.81219480965214 102-103 22.412855103519135 28.281288359363398 26.658488387247342 22.64736814987013 104-105 22.160648868987412 28.400140926706747 26.525967261059147 22.913242943246694 106-107 22.12916503124105 28.574307930816573 26.444567027697925 22.851960010244458 108-109 22.26452462454408 28.522906182616058 26.281005135385367 22.931564057454494 110-111 22.09833993867675 28.82639551296281 26.407786703599008 22.667477844761425 112-113 22.10072343636821 28.95851833442945 26.358905662321604 22.581852566880737 114-115 21.98380128198815 28.941552946595444 26.284014694150905 22.790631077265505 116-117 21.862829099630833 28.976676568159316 26.499788258056988 22.660706074152863 118-119 22.030823786142935 28.9558707643814 26.173319391404497 22.839986058071165 120-121 21.919140872719723 29.02221538643377 26.378356344769223 22.680287396077283 122-123 21.982332128705746 29.05251115606367 26.401951648218443 22.563205067012145 124-125 22.1607361758676 28.94987658174622 26.29255808385774 22.596829158528436 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1395.0 1 1299.5 2 1192.5 3 1029.5 4 712.5 5 475.5 6 384.5 7 356.5 8 337.0 9 346.5 10 312.5 11 255.5 12 259.0 13 261.0 14 261.0 15 300.5 16 343.0 17 371.0 18 441.5 19 526.0 20 649.5 21 866.5 22 1129.5 23 1458.0 24 1936.0 25 2495.5 26 3311.0 27 4252.5 28 5172.0 29 6257.5 30 7228.5 31 8423.0 32 10162.5 33 11569.0 34 12614.0 35 13583.0 36 15068.0 37 16498.5 38 17220.0 39 18136.0 40 19160.0 41 20308.0 42 21375.0 43 22193.0 44 22907.5 45 23557.5 46 24022.5 47 24212.5 48 24267.0 49 24529.0 50 24504.5 51 24058.0 52 23594.0 53 23126.5 54 22702.5 55 21574.0 56 19763.5 57 17949.5 58 16571.5 59 14740.0 60 12357.0 61 10304.5 62 8390.5 63 6295.5 64 4591.5 65 3554.0 66 2695.0 67 2097.5 68 1575.0 69 1130.5 70 857.5 71 659.0 72 524.5 73 450.5 74 361.0 75 285.0 76 208.0 77 115.0 78 74.5 79 57.5 80 36.0 81 23.0 82 18.5 83 17.5 84 11.5 85 6.0 86 6.5 87 5.0 88 3.5 89 3.5 90 6.5 91 5.5 92 3.5 93 3.5 94 2.5 95 2.0 96 2.5 97 2.0 98 1.0 99 2.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05060092384560961 2 0.20240369538243844 3 0.02742145873070461 4 0.10559534107901168 5 0.02408846374686206 6 0.038480942086182156 7 0.04090493843806765 8 0.10408034335908324 9 0.1218058166822459 10-11 0.04567718125584221 12-13 0.030375704284565052 14-15 0.012271481531420296 16-17 0.030527204056557893 18-19 0.053555169399470054 20-21 0.18785971727112552 22-23 0.10673158936895799 24-25 0.07362888918852177 26-27 0.014695477883305786 28-29 0.0057569913357280395 30-31 0.008105237801617109 32-33 0.0058327412217244615 34-35 0.008256737573609951 36-37 0.0037874942998210783 38-39 0.008786986775584902 40-41 0.0057569913357280395 42-43 0.009923235065531227 44-45 0.009468735749552697 46-47 0.016058975831241374 48-49 0.016134725717237796 50-51 0.010150484723520491 52-53 0.012953230505388089 54-55 0.012725980847398826 56-57 0.014468228225316522 58-59 0.01575597628725569 60-61 0.00719623916966005 62-63 0.017422473779176963 64-65 0.030072704740579364 66-67 0.025300461922804805 68-69 0.031663452346504216 70-71 0.02355821454488711 72-73 0.0143924783393201 74-75 0.00916573620556701 76-77 0.009695985407541961 78-79 0.023406714772894268 80-81 0.018785971727112553 82-83 0.019391970815083922 84-85 0.014165228681330836 86-87 0.023255215000901424 88-89 0.02567921135278691 90-91 0.025224712036808386 92-93 0.0298454550825901 94-95 0.02348246465889069 96-97 0.025603461466790493 98-99 0.031208953030525687 100-101 0.019770720245066032 102-103 0.028633456906647353 104-105 0.02264921591293005 106-107 0.029996954854582945 108-109 0.020603968991026667 110-111 0.018407222297130443 112-113 0.024391463290847747 114-115 0.011589732557452501 116-117 0.009923235065531227 118-119 0.02833045736266167 120-121 0.021437217736987306 122-123 0.01696797446319843 124-125 0.017573973551169804 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 660067.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.72691501254316 #Duplication Level Percentage of deduplicated Percentage of total 1 79.35388759711887 52.95040113607854 2 12.531316176277235 16.723521389795167 3 3.8919824545975112 7.790999474347117 4 1.6113794877899625 4.300895285388646 5 0.8124290100917895 2.710544075505971 6 0.4623498797724843 1.8510708680182861 7 0.30451938671420903 1.422374746586555 8 0.19802295681684098 1.0570768808039883 9 0.1405474910388683 0.8440470440799063 >10 0.6547008809944722 7.416354608358909 >50 0.02500077998347316 1.1128550867923663 >100 0.013636621774747063 1.5582546149160104 >500 0.0 0.0 >1k 2.2727702952678983E-4 0.2616047893285196 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1725 0.2613371066876544 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.5149977199284314E-4 0.0 16-17 0.0 0.0 0.0 2.272496579892647E-4 0.0 18-19 0.0 0.0 0.0 3.7874942998210786E-4 0.0 20-21 0.0 0.0 0.0 4.5449931597852945E-4 0.0 22-23 0.0 0.0 0.0 4.5449931597852945E-4 0.0 24-25 0.0 0.0 0.0 6.817489739677941E-4 0.0 26-27 0.0 0.0 0.0 0.001060498403949902 0.0 28-29 0.0 0.0 0.0 0.0016664974919212748 0.0 30-31 0.0 0.0 0.0 0.003408744869838971 0.0 32-33 0.0 0.0 0.0 0.008483987231599217 0.0 34-35 0.0 0.0 0.0 0.014695477883305785 0.0 36-37 0.0 0.0 0.0 0.024770212720829854 0.0 38-39 0.0 0.0 0.0 0.043783434105931676 0.0 40-41 0.0 0.0 0.0 0.068099147510783 0.0 42-43 0.0 0.0 0.0 0.0905968636517202 0.0 44-45 0.0 0.0 0.0 0.11673057432048564 0.0 46-47 0.0 0.0 0.0 0.15013627404490756 0.0 48-49 0.0 0.0 0.0 0.1881627168151112 0.0 50-51 0.0 0.0 0.0 0.22861315593720033 0.0 52-53 0.0 0.0 0.0 0.2699725936912465 0.0 54-55 0.0 0.0 0.0 0.31761927198299567 0.0 56-57 0.0 0.0 0.0 0.3726136892163977 0.0 58-59 0.0 0.0 0.0 0.4329863483555457 0.0 60-61 0.0 0.0 0.0 0.49904024894442534 0.0 62-63 0.0 0.0 0.0 0.5657001486212763 0.0 64-65 0.0 0.0 0.0 0.6297088022882525 0.0 66-67 0.0 0.0 0.0 0.7078826846365596 0.0 68-69 0.0 0.0 0.0 0.8009792945261618 0.0 70-71 0.0 0.0 0.0 0.9073321344651376 0.0 72-73 0.0 0.0 0.0 1.0205024641437914 0.0 74-75 0.0 0.0 0.0 1.1280673022587102 0.0 76-77 0.0 0.0 0.0 1.2406316328493925 0.0 78-79 0.0 0.0 0.0 1.3835716677246401 0.0 80-81 0.0 0.0 0.0 1.548857918968832 0.0 82-83 0.0 0.0 0.0 1.7208101601807089 0.0 84-85 0.0 0.0 0.0 1.8864751608548829 0.0 86-87 0.0 0.0 0.0 2.0597909000146952 0.0 88-89 0.0 0.0 0.0 2.2596190992732557 0.0 90-91 0.0 0.0 0.0 2.490353252018356 0.0 92-93 0.0 0.0 0.0 2.7341163851548407 0.0 94-95 0.0 0.0 0.0 2.9850000075749885 0.0 96-97 0.0 0.0 0.0 3.2351261311351727 0.0 98-99 0.0 0.0 0.0 3.503962476536473 0.0 100-101 0.0 0.0 0.0 3.818627502965608 0.0 102-103 0.0 0.0 0.0 4.163440984021319 0.0 104-105 0.0 0.0 0.0 4.511663209946869 0.0 106-107 0.0 0.0 0.0 4.856855440432563 0.0 108-109 0.0 0.0 0.0 5.219318644925439 0.0 110-111 0.0 0.0 0.0 5.623595786488341 0.0 112-113 7.574988599642157E-5 0.0 0.0 6.079155600870821 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 835 0.0 56.44659 1 GTATCAA 1440 0.0 51.78985 1 ATCAACG 1620 0.0 43.033558 3 TCAACGC 1635 0.0 41.91307 4 CAACGCA 1650 0.0 41.519432 5 AACGCAG 1805 0.0 37.62402 6 TATCAAC 1975 0.0 36.54125 2 GTACATG 3640 0.0 35.895485 1 TACATGG 3660 0.0 35.525528 2 ACGCAGA 2105 0.0 31.695929 7 ACATGGG 3775 0.0 30.77897 3 CGCAGAG 2170 0.0 30.74651 8 GTACTAG 195 1.378794E-9 30.595848 1 GAGTACA 2550 0.0 26.67238 1 GCAGAGT 2530 0.0 25.19039 9 AGTACAT 2585 0.0 24.688139 2 CATGGGA 2230 0.0 24.583988 4 CATGGGG 1695 0.0 23.906092 4 GGACCGT 125 0.0016214376 23.828545 6 ATTCGCG 65 0.0020574736 22.874746 80-81 >>END_MODULE